牛病毒性腹泻病毒检测技术研究进展
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摘要
牛病毒性腹泻(BVD)是由牛病毒性腹泻病毒(BVDV)所致牛的一种接触性传染病,呈世界性流行,该病主要表现为腹泻、急慢性黏膜感染、繁殖障碍等,对畜牧业危害严重,是国际贸易中动物检疫的重要疫病之一。快速准确的检测手段,有利于该传染病的早期发现和及时控制。目前,用于检测牛病毒性腹泻病毒的方法很多,论文对近年来国内外应用的等温核酸扩增技术(IAT)、重组酶聚合酶扩增技术(RPA)、指数富集配体系统进化技术(SELEX)、多重PCR技术、多重连接探针扩增技术(MLPA)、RT-PCR抗原酶联免疫吸附试验(ELISA)和间接免疫荧光试验(IFA)等进行简要介绍,为我国动物检疫工作提供参考。
Bovine viral diarrhea is caused by bovine viral diarrhea virus,which manifests many symptoms,such as diarrhea,acute and chronic mucosal infection and reproductive disturbance.It is seriously harms to animal husbandry and also one of the important animal diseases of animal quarantine in international trade.Rapid and accurate detection is conducive to early detection and control of this disease.At present,there are many methods for detecting bovine viral diarrhea virus.In this paper,we introduced the detection technologies including IAT,RPA,SELEX,multiplex PCR,MLPA,RT-PCR ELISA and IFA,in order to provide reference for rapid and highly effective detecting techniques in the work of animal inspection and quarantine.
Bovine viral diarrhea is caused by bovine viral diarrhea virus,which manifests many symptoms,such as diarrhea,acute and chronic mucosal infection and reproductive disturbance.It is seriously harms to animal husbandry and also one of the important animal diseases of animal quarantine in international trade.Rapid and accurate detection is conducive to early detection and control of this disease.At present,there are many methods for detecting bovine viral diarrhea virus.In this paper,we introduced the detection technologies including IAT,RPA,SELEX,multiplex PCR,MLPA,RT-PCR ELISA and IFA,in order to provide reference for rapid and highly effective detecting techniques in the work of animal inspection and quarantine.
引文
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[19]宋爽,赵柏林,曲萍,等.牛病毒性腹泻病毒、牛传染性支气管炎病毒和口蹄疫病毒多重TaqMan荧光定量PCR检测方法的建立[J].中国预防兽医学报,2017,39(2):133-136.
[20] Schouten J P,Mcelgunn C J,Waaijer R,et al.Relative quantification of 40nucleic acid sequences by multiplex ligation-dependent probe amplification[J].Nucl Acid Res,2002,30(12):e57.
[21]史喜菊,马贵平,柏亚铎,等.多重连接探针扩增对5种牛病毒同步检测方法的建立[J].中国兽医学报,2017,37(2):224-230.
[22] Dubey P,Mishra N,Rajukumar K,et al.Development of a RT-PCR ELISA for simultaneous detection of BVDV-1,BVDV-2and BDV in ruminants and its evaluation on clinical samples[J].J Virol Meth,2015,213:50-56.
[23]黄杰,岳丰雄.牛病毒性腹泻病毒间接免疫荧光检测方法的建立及应用[J].甘肃畜牧兽医,2017,47(3):69-71.
[24] Bedekovi8T,Lemo N,Lojki8I,et al.Development of an indirect immunofluorescence assay for diagnosis of bovine viral diarrhoea virus on ear notch tissue samples in cattle infected persistently[J].J Virol Meth,2011,178(1-2):59-62
[2] Deng M,Ji S,Fei W,et al.Prevalence study and genetic typing of bovine viral diarrhea virus(BVDV)in four bovine species in China[J].PLoS One,2015,10(4):e0121718.
[3] Nathalie C,Britta B,Pierre B,et al.Morphology and molecular composition of purified bovine viral diarrhea virus envelope[J].PLoS Pathog,2016,12(3):e1005476.
[4] Couvreur B,Letellier C,Collard A,et al.Genetic and antigenic variability in bovine viral diarrhea virus(BVDV)isolates from Belgium[J].Viru Res,2002,85(1):17.
[5]范晴,谢芝勋,谢志勤,等.牛病毒性腹泻病毒抗原捕获ELISA方法的建立[J].动物医学进展,2015,36(2):21-24.
[6] Aduriz G,Atxaerandio R,Cortabarria N.First detection of bovine viral diarrhoea virus type 2in cattle in Spain[J].Vet Rec Open,2015,2(1):e000110.
[7]黄杰,岳丰雄,徐宏军,等.牛病毒性腹泻病毒实时荧光定量RT-PCR检测方法的建立[J].动物医学进展,2017,38(5):59-63.
[8] Notomi T,Okayama H,Masubuchi H,et al.Loop-mediated isothermal amplification of DNA[J].Nuc Acid Res,2000,28(12):E63.
[9]李家伟,郭利,杨勇,等.牛病毒性腹泻病毒LAMP检测方法的建立与应用[J].中国畜牧兽医,2015,42(12):3111-3118.
[10] Rezatofighi S E,Tajbakhsh A,Mirzadeh K,et al.A reverse transcriptase-loop mediated isothermal amplification assay(RT-LAMP)for rapid detection of bovine viral diarrhea virus1and 2[J].Arch Razi Inst,2017,72(2):73-81.
[11]段进刚,樊新丽,葛婷,等.荧光定量RT-PCR和RT-LAMP检测BVDV方法的建立及应用比较[J].黑龙江畜牧兽医,2017(19):170-174,179.
[12] Xu G,Hu L,Zhong H,et al.Cross priming amplification:mechanism and optimization for isothermal DNA amplification[J].Sci Rep,2012,2(2):246.
[13] Kuta A,Wo6niakowski G Polak M P.Cross-priming amplification for detection of bovine viral diarrhoea virus species 1and 2[J].J Appl Microbiol,2015,119(3):632.
[14] Piepenburg O,Williams C H,Stemple D L,et al.DNA detection using recombination proteins[J].PLoS Biol,2006,4(7):1115-1121.
[15]候佩莉.牛病毒性腹泻病毒RPA-LFD快速检测方法的建立[C]//中国畜牧兽医学会生物技术学分会暨中国免疫学会兽医免疫分会第十二次学术研讨会论文集,云南昆明:中国畜牧兽医学会生物技术学分会,2016.
[16] Tuerk C,Gold L.Systematic evolution of ligands by exponential enrichment:RNA ligands to bacteriophage T4DNA polymerase[J].Science,1990,249(4968):505-510.
[17] Park J W,Jin L S,Choi E J,et al.An ultra-sensitive detection of a whole virus using dual aptamers developed by immobilization-free screening[J].Biosensors Bioelect,2014,51(2):324.
[18]侯佩莉,王洪梅,何洪彬.牛病毒性腹泻病毒、牛冠状病毒和牛肠道病毒多重RT-PCR检测方法的建立及初步应用[J].中国兽医学报,2016,36(10):1672-1675.
[19]宋爽,赵柏林,曲萍,等.牛病毒性腹泻病毒、牛传染性支气管炎病毒和口蹄疫病毒多重TaqMan荧光定量PCR检测方法的建立[J].中国预防兽医学报,2017,39(2):133-136.
[20] Schouten J P,Mcelgunn C J,Waaijer R,et al.Relative quantification of 40nucleic acid sequences by multiplex ligation-dependent probe amplification[J].Nucl Acid Res,2002,30(12):e57.
[21]史喜菊,马贵平,柏亚铎,等.多重连接探针扩增对5种牛病毒同步检测方法的建立[J].中国兽医学报,2017,37(2):224-230.
[22] Dubey P,Mishra N,Rajukumar K,et al.Development of a RT-PCR ELISA for simultaneous detection of BVDV-1,BVDV-2and BDV in ruminants and its evaluation on clinical samples[J].J Virol Meth,2015,213:50-56.
[23]黄杰,岳丰雄.牛病毒性腹泻病毒间接免疫荧光检测方法的建立及应用[J].甘肃畜牧兽医,2017,47(3):69-71.
[24] Bedekovi8T,Lemo N,Lojki8I,et al.Development of an indirect immunofluorescence assay for diagnosis of bovine viral diarrhoea virus on ear notch tissue samples in cattle infected persistently[J].J Virol Meth,2011,178(1-2):59-62