甲基丙烯酸树脂吸附微生物降解与演化石油烃特性
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摘要
为了提高石油污染土壤的生物降解效率,以甲基丙烯酸丁酯制备的树脂为载体吸附石油降解菌进行生物降解,利用GC-MS"指纹图谱"对TPH(total petroleum hydrocarbon,总石油烃)的降解程度及生物演化特性进行分析.结果表明:树脂吸附菌剂后TPH的半衰期为24.23 d,比单纯菌剂降解缩短7.28 d;其中正构烷烃的降解性能表明,高碳数高达92.03%,中、低碳数皆超过75%,高于TPH的56.67%,较好地解释了TPH降解率比单纯菌剂提高的内在原因;在演化特征方面,正构烷烃的生物演化参数———w(∑C_(21))/w(∑C_(22+))(C_(21-)、C_(22+)分别指C_(14)～C_(21)和C_(22)～C_(39))、姥植比[w(pr)/w(ph)]和OEP(奇/偶碳数质量比)分别为0.643 3、0.486 5和1.111 9,均高于单纯菌剂演化程度.研究显示,树脂吸附菌剂后对较难降解的偶数碳正构烷烃和类异戊二烯烃类物质的氧化还原能力增强,从生物演化的角度有力地佐证了降解的优势所在.
In order to improve the biodegradation efficiency of petroleum-contaminated soil,biological degradation was studied with oildegradation bacteria adsorbed on resin(ODB-AOR)that was made of butyl methacrylate.The degradation of TPH(Total Petroleum Hydrocarbon)and the biological transformation characteristics were analyzed through a GC-MS fingerprint.The results showed that the half-life of TPH in ODB-AOR was 24.23 d,which was 7.28 days shorter than using the pure bacterium.The degradation rate of alkanes with a high carbon number reached 92.03%,and rate of alkanes with low/medium numbers was greater than 75%,which was significantly higher than the degradation rate of TPH(56.67%).It's why the degradation rate of TPH was greater than pure bacterium.The study on transformation characteristics of ODB-AOR showed that biological marker ratio of normal alkanes of high carbon/low carbon,pristane/phytane,and odd/even were 0.6433,0.4865 and 1.1119,respectively,which were all higher than the degradation of the pure bacterium.The results indicated that ODB-AOR could significantly enhance the ability of oxidation-reduction for n-alkanes in even-numbered carbon and isoprenoid hydrocarbon,which proves the advantages of the degradation on the basis of biological evolution.
In order to improve the biodegradation efficiency of petroleum-contaminated soil,biological degradation was studied with oildegradation bacteria adsorbed on resin(ODB-AOR)that was made of butyl methacrylate.The degradation of TPH(Total Petroleum Hydrocarbon)and the biological transformation characteristics were analyzed through a GC-MS fingerprint.The results showed that the half-life of TPH in ODB-AOR was 24.23 d,which was 7.28 days shorter than using the pure bacterium.The degradation rate of alkanes with a high carbon number reached 92.03%,and rate of alkanes with low/medium numbers was greater than 75%,which was significantly higher than the degradation rate of TPH(56.67%).It's why the degradation rate of TPH was greater than pure bacterium.The study on transformation characteristics of ODB-AOR showed that biological marker ratio of normal alkanes of high carbon/low carbon,pristane/phytane,and odd/even were 0.6433,0.4865 and 1.1119,respectively,which were all higher than the degradation of the pure bacterium.The results indicated that ODB-AOR could significantly enhance the ability of oxidation-reduction for n-alkanes in even-numbered carbon and isoprenoid hydrocarbon,which proves the advantages of the degradation on the basis of biological evolution.
引文
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[25] ZHU Kun,WILLIAM H A R T,YANG Jiantao. Remediation of petroleum-contaminated loess soil by surfactant-enhanced flushing technique[J]. Journal of Environmental Science,2013,40(11):1877-1893.
[26] SEO J S,KEUM Y S,LI Q X. Bacterial degradation of aromatic compounds[J]. International Journal of Environmental Research and Public Health,2009,49(6):278-309.
[27] TAHA R M,OMAR B A,PILLAY A E,et al. Recycling of petroleum-contaminated sand[J]. The Royal Society of Chemistry,2001,67(3):417-420.
[28] MILLS M A,BONNER J S,THOMAS J,et al. Intrinsic bioremediation of a petroleum-impacted wetland[J]. Marine Pollution Bulletin,2003,46(3):887-899.
[29] BASSAM M,MOHAMMED N B. Biodegradation of total organic carbons(TOC)in Jordanian petroleum sludge[J]. Journal of Hazardous Materials,2005,120(5):127-134.
[30] WHYTE L G,BOURBONNIERE L,GREER C W.Biodegradation of petroleum hydrocarbons by psychrotrophic Pseudomonas strains possessing both alkane and naphthalene catabolic pathways[J].Applied and Environmental Microbiology,1997,63(9):3719-3723.
[2] ALLEBRANDT S R,NAPP A P,MITIDIERI S,et al.Bioremediation of diesel oil-contaminated soil using native hydrocarbon-degrading bacterial strains under microcosm study[J].International Journal of Advanced Research,2015,3(11):732-746.
[3] KISHORE E D,ASHIS K M. Crude petroleum-oil biodegradation efficiency of bacillus subtilis and pseudomonas aeruginosa strains isolated from a petroleum-oil contaminated soil from North-East India[J].Bioresource Technology,2007,98(7):1339-1345.
[4] SUMAIYA A K,ABED R M M. Effect of biostimulation using sewage sludge,soybean meal,and wheat straw on oil degradation and bacterial community composition in a contaminated desert soil[J].Frontiers in Microbiology,2016,2836(7):1-14.
[5] ROCCHETTI L,BEOLCHINI F,CIANI M,et al. Improvement of bioremediation performance for the degradation of petroleum hydrocarbons in contaminated sediments[J]. Applied&Environmental Soil Science,2011,2011(2):1-8.
[6] SCHINK B. Synergstic interactions in the microbial world[J].Antonie Van Leeuwenhoek,2002,81(5):257-261.
[7] BOUCHEZ T,PATUREAU D,DABERT P,et al.Ecological study of a bioaugmentation failure[J].Environmental Microbiology,2000,435(2):179-190.
[8]韩慧龙,汤晶,刘铮,等.真菌-细菌修复石油污染土壤的协同作用机制研究[J].环境科学,2008,29(1):189-195.HAN Huilong,TANG Jing,LIU Zheng,et al.Synergy between fungi and bacteria in fungi-bacteria augmented remediation of petroleumcontaminated soil[J]. Environmental Science,2008,29(1):189-195.
[9]牛秋雅,曾光明,牛一乐,等.臭氧预氧化-堆肥去除污染土壤中菲的试验研究[J].环境科学学报,2009,29(11):2352-2358.NIU Qiuya,ZENG Guangming,NIU Yile,et al. Removal of phenanthrene from contain mated soil by a combination of preozonation and composting[J]. Acta Scientiae Circumstantiae,2009,29(11):2352-2358.
[10]陈丽华,马金珠,雒晓芳,等.添加混合菌剂对石油污染土壤的降解研究[J].中南大学学报,2012,43(11):4181-4189.CHEN Lihua,MA Jinzhu,LUO Xiaofang,et al. Degrading of petroleum contaminated soil by injected mixed oildegradation bacterial stains[J]. Journal of Central South University,2012,43(11):4181-4189.
[11] ANTONIOU E,FODELIANAKIS S,KORKAKAKI E,et al.Biosurfactant production from marine hydrocarbon-degrading consortia and pure bacterial strains using crude oil as carbon source[J].Frontiers in Microbiology,2015,274(6):274-282.
[12] AYOTAMUNO M J,KOGBARA R B,OGAJS O T,et al.Bioremediation of a crude-oil polluted agricultural-soil at Port Harcourt,Nigeria[J].Applied Energy,2006,83(11):1249-1257.
[13]张秀霞,武海杰,韩雨彤,等.改性秸秆载体固定化微生物修复石油污染土壤[J].石油学报,2014,30(5):915-920.ZHANU Xiuxia,WU Haijie,HAN Yutong,et al. Remediation of petroleum contaminated soil by modified straw carrier immobilized microorganism[J].Acta Petrolei Sinica,2014,30(5):915-920.
[14]王冬梅,陈丽华,雒晓芳,等.鼠李糖脂对微生物菌剂降解石油的影响[J].环境工程学报,2013,7(10):4121-4126.WANG Dongmei,CHEN Lihua,LUO Xiaofang,et al. Effects of rhamnolipid on petroleum degradation of compound microbial inoculant[J].Chinese Journal of Environmental Engineering,2013,7(10):4121-4126.
[15]陆凡,胡清华,李廷强.生物表面活性剂皂角苷对柴油污染土壤脱附的强化作用[J].农业环境科学学报,2015,34(1):37-43.LU Fan,HU Qinghua,LI Tingqiang. Enhancing removal efficiency of diesel contaminant from soil by biosurfactant saponin[J].Journal of Agro-Environment Science,2015,34(1):37-43.
[16] LU L,HUGGINS T,JIN S,et al. Microbial metabolism and community structure in response to bioelectrochemical enhanced remediation of petroleum hydrocarbon-contaminated soil[J].Environmental Science&Technology,2014,48(1):4021-4029.
[17]王坚,张旭,李广贺.油田污染土壤残油组成与特征参数分析[J].环境科学,2012,3(4):1353-1357.WANG Jian,ZHANG Xu,LI Guanghe.Compositions and diagnostic ratios of heavily degraded crude oil residues in contaminated soil in oilfields[J].Environmental Science,2012,33(4):1353-1357.
[18] SOLIMAN F M,YANG W,GUO H,et al. Synthesis and characterization of an ionic liquid enhanced high oil absorption resin of P(BMlm-MMA-BA)and its oil absorption performance[J]Science Journal of Chemistry,2016,4(5):61-68.
[19]王震宇,许颖,高冬梅,等.堆制技术对土壤中石油烃的降解研究[J].环境科学与技术,2010,33(9):1-6.WANG Zhenyu,XU Ying,GAO Dongmei,et al. Bioremediation of petroleum contaminated soil by composting[J]. Environmental Science&Technology(China),2010,33(9):1-6.
[20]陈丽华,孙万虹,李海玲,等.石油降解菌对石油烃中不同组分的降解及演化特征研究[J].环境科学学报,2016,35(1):125-133.CHEN Lihua,SUN Wanhong,LI Hailing,et al. Biological degradation and transformation characteristics of different components in petroleum hydrocarbon by oil-degradation bacteria[J].Acta Scientiae Circumstantiae,2016,35(1):125-133.
[21] CUI Wenbo,LI Yunhui,MA Yuqin,et al. Research on the dehydration property of one of super absorbent resin on the swill oil[J]. Chemical Journal of Chinese Universities,2014,35(12):2510-2515.
[22] ZAFIROG L U,DIMITR I P.Article for absorbing oil:US,4737394[P].1988-04-12.
[23]王丽娟,王哲,张翼龙,等.土壤中石油烃降解菌群的构建及其在降解过程的特征分析[J].安徽农业科学,2010,38(20):10834-10836.WANG Lijuan,WANG Zhe,ZHANG Yilong,et al. Construction of the flora degrading oil in the soil and characreristics analysis in the degradation process[J]. Journal of Anhui Agricultural Sciences,2010,38(20):10834-10836.
[24]陈立,万力,张发旺,等.土著微生物原位修复石油污染土壤试验研究[J].生态环境学报,2010,19(7):1686-1690.
[25] ZHU Kun,WILLIAM H A R T,YANG Jiantao. Remediation of petroleum-contaminated loess soil by surfactant-enhanced flushing technique[J]. Journal of Environmental Science,2013,40(11):1877-1893.
[26] SEO J S,KEUM Y S,LI Q X. Bacterial degradation of aromatic compounds[J]. International Journal of Environmental Research and Public Health,2009,49(6):278-309.
[27] TAHA R M,OMAR B A,PILLAY A E,et al. Recycling of petroleum-contaminated sand[J]. The Royal Society of Chemistry,2001,67(3):417-420.
[28] MILLS M A,BONNER J S,THOMAS J,et al. Intrinsic bioremediation of a petroleum-impacted wetland[J]. Marine Pollution Bulletin,2003,46(3):887-899.
[29] BASSAM M,MOHAMMED N B. Biodegradation of total organic carbons(TOC)in Jordanian petroleum sludge[J]. Journal of Hazardous Materials,2005,120(5):127-134.
[30] WHYTE L G,BOURBONNIERE L,GREER C W.Biodegradation of petroleum hydrocarbons by psychrotrophic Pseudomonas strains possessing both alkane and naphthalene catabolic pathways[J].Applied and Environmental Microbiology,1997,63(9):3719-3723.