摘要
目的建立牛筋草中总黄酮的测定方法,为其质量评价提供理论依据。方法采用紫外-可见分光光度(UV-VIS)法,检测波长507 nm。结果牛筋草总黄酮在0.0042~0.0954 mg/ml范围内线性关系良好(Y=12.342X+0.0082,r=0.9998,n=6),平均回收率为99.51%,RSD为3.35%。结论本方法简单、方便、专属性强,可为牛筋草药材的质量控制提供参考。
Objective Ultraviolet-Visible Spectrophotometry was established to determine the total flavonoids content in Eleusine indica(L.)Gaertn. Methods Reference standard method(λ-507nm) was used to determine the content of total flavonoids in Eleusine indica(L.)Gaertn. Results The test of Soluble protein in Eleusine indica(L.)Gaertn. was linear within the range of 0.0042~0.0954 mg·ml-1(Y=12.342X+0.0082, r=0.9998, n=6).The average recovery was 99.51%,RSD=3.35%. Conclusion The methods were simple and accurate.
引文
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