柴胡龙骨牡蛎汤对心肌梗死合并抑郁大鼠药效学作用的实验研究
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摘要
目的:观察柴胡龙骨牡蛎汤对心肌梗死后伴发抑郁大鼠的药效学作用。方法:将68只大鼠随机分为正常组、模型对照组、西药组和中药组。给予模型对照组、西药组和中药组大鼠先后制造心肌梗死和抑郁症模型,造模成功后,正常组、模型对照组大鼠灌服生理盐水,西药组大鼠灌服百忧解,中药组大鼠灌服中汤剂柴胡龙骨牡蛎汤,连续给药28 d。分别记录各组大鼠敞箱试验评分和游泳不动时间。给药结束后测量大鼠左、右心室质量、体质量、室间隔厚度,计算大鼠左、右心室质量与体质量比值;高效液相色谱法检测大鼠脑组织5-羟色胺(5-HT)含量;酶联免疫法检测大鼠血浆血管紧张素-Ⅱ(AgⅡ)和醛固酮(ALD)含量。结果:模型组大鼠的旷场试验评分和脑组织中5-HT含量均低于正常组,游泳不动时间长于正常组,血浆AgⅡ和ALD含量、左心室质量/体质量、右心室质量/体质量、室间隔厚度均高于正常组(P <0. 05)。西药组和中药组大鼠敞箱试验评分和脑组织中5-HT含量高于模型组,左心室质量/体质量、右心室质量/体质量、室间隔厚度、游泳不动时间、血浆AgⅡ和ALD含量低于模型组(P <0. 05);西药组和中药组之间无显著差异。结论:柴胡龙骨牡蛎汤可有效改善心肌梗死后并发抑郁大鼠的抑郁症状,并且能够逆转心肌梗死大鼠的心肌重塑。
Objective: To observe the pharmacodynamic actions of Chaihu Longgu Muli Tang on rats of depression after myocardial infarction. Methods: 68 rats were divided into 4 groups randomly: normal group,model group,Chinese medicine group and Western medicine group. Rats were made myocardial infarction model and depression model successively except for normal group. The rats of normal and model group were given 0. 9% sodium chloride solution. The rats in Chinese medicine group were given Chaihu Longgu Muli Tang and the rats of Western medicine group were given Baiyoujie for 28 days. Open-field scores and swimming no-moving time were tested. The ratio of left-ventricular to body weight and right-ventricular to body weight were calculated and the thickness of ventricular was measured. High performance liquid chromatography( HPLC) method was used to test the levels of 5-HT in brain,and the levels of plasma angiotensin Ⅱ( AgⅡ) and aldosterone( ALD) were measured with enzyme-linked immunosorbent assay( ELISA) methods. Results: Compared with normal group,open-field scores and 5-HT content in brain of model rats were reduced,swimming no-moving time was prolonged significantly,ratio of left-ventricular to body weight and right-ventricular to body weight were raised and plasma AgⅡ and ALD levels were increased. To compare with the model group,open-field scores and 5-HT content in brain of Chinese and Western medicine groups rats were increased,swimming no-moving time was shortened,ratio of left-ventricular to body weight and right-ventricular to body weight were decreased and plasma AgⅡ and ALD levels were lowered,but there were no significant difference between Chinese and Western medicine groups. Conclusion: Chaihu Longgu Muli Tang can effectively improve symptoms of depression after myocardial infarction rats,and reverse rats' ventricular remodeling.
Objective: To observe the pharmacodynamic actions of Chaihu Longgu Muli Tang on rats of depression after myocardial infarction. Methods: 68 rats were divided into 4 groups randomly: normal group,model group,Chinese medicine group and Western medicine group. Rats were made myocardial infarction model and depression model successively except for normal group. The rats of normal and model group were given 0. 9% sodium chloride solution. The rats in Chinese medicine group were given Chaihu Longgu Muli Tang and the rats of Western medicine group were given Baiyoujie for 28 days. Open-field scores and swimming no-moving time were tested. The ratio of left-ventricular to body weight and right-ventricular to body weight were calculated and the thickness of ventricular was measured. High performance liquid chromatography( HPLC) method was used to test the levels of 5-HT in brain,and the levels of plasma angiotensin Ⅱ( AgⅡ) and aldosterone( ALD) were measured with enzyme-linked immunosorbent assay( ELISA) methods. Results: Compared with normal group,open-field scores and 5-HT content in brain of model rats were reduced,swimming no-moving time was prolonged significantly,ratio of left-ventricular to body weight and right-ventricular to body weight were raised and plasma AgⅡ and ALD levels were increased. To compare with the model group,open-field scores and 5-HT content in brain of Chinese and Western medicine groups rats were increased,swimming no-moving time was shortened,ratio of left-ventricular to body weight and right-ventricular to body weight were decreased and plasma AgⅡ and ALD levels were lowered,but there were no significant difference between Chinese and Western medicine groups. Conclusion: Chaihu Longgu Muli Tang can effectively improve symptoms of depression after myocardial infarction rats,and reverse rats' ventricular remodeling.
引文
[1]刘梅颜,胡大一.抑郁症与缺血性心脏病[J].中国医药导刊,2005,7(4):271-274.
[2]Van Melle JP,De Jonge P,Kuyper AMG,et al. Prediction of depressive disorder following myocardial infarction:Data from the myocardial infarction and depression-infarction and depression-intervention trial(MINDIT)[J]. Cardiology,2006,2(9):36-37.
[3]Post-Myocardial Infarction Depression Clinical Pracice Guideline Panel. AAFP guideline for the detection and management of postmyocardial infarction depression[J].Ann Fam Med,2009,7(1):71-79.
[4]Dickens C,McGowan L,Percival C,et al. New onset depression following myocardial infarction predicts cardiac mortality[J]. Psychosom Med,2008,70(4):450-455.
[5]Watlins L L,Grossman P. Association of depressive symptoms with reduced baroreflex cardiac control in coronary artery disease[J]. Am Heart J,2005,37(3):453-457.
[6]梁开斌,马现涛.加减柴胡龙骨牡蛎汤队冠心病并发抑郁焦虑患者中医症候及负性心理的影响[J].中西医结合心脑血管病杂志,2017,5(31):137-138.
[7]Shi S,Liang J,Liu T,et al. Depression increases sympathetic activity and exacerbates myocardial remodeling after myocardial infarction:evidence from an animal experiment[J]. PLo S One,2014,9(7):e101734.
[8]Reese RL,Freedland KE,Steinmeyer BC,et al. Depression and rehospitalization following acute myocardial infarction[J]. Circ Cardiovasc Qual Outcomes,2011,4(6):626-633.
[9]黄佐,樊民,吴宗贵.冠心病患者抑郁症状与冠状动脉病变的关系[J].中华心血管病杂志,2005,9(4):329.
[10]Carney RM,Biumenthal JA,Freedland KE,et a1. Low heart rate variability and the effect of depression on post-myocardial infarction mortality[J]. Arch Intem Med,2005,165:1486.
[11]吴大兴.综合医院心理联络会诊[M].北京:人民卫生出版社,2011:131-137.
[12]Cooper GI. The safety of fluoxctine-anupdate[J]. Br J Psychiatry,2003,153:77-86
[13]包祖晓,管利民.柴胡加龙骨牡蛎汤在神经精神疾病中的运用[J].河北中医,2003,25(11):838-839.
[2]Van Melle JP,De Jonge P,Kuyper AMG,et al. Prediction of depressive disorder following myocardial infarction:Data from the myocardial infarction and depression-infarction and depression-intervention trial(MINDIT)[J]. Cardiology,2006,2(9):36-37.
[3]Post-Myocardial Infarction Depression Clinical Pracice Guideline Panel. AAFP guideline for the detection and management of postmyocardial infarction depression[J].Ann Fam Med,2009,7(1):71-79.
[4]Dickens C,McGowan L,Percival C,et al. New onset depression following myocardial infarction predicts cardiac mortality[J]. Psychosom Med,2008,70(4):450-455.
[5]Watlins L L,Grossman P. Association of depressive symptoms with reduced baroreflex cardiac control in coronary artery disease[J]. Am Heart J,2005,37(3):453-457.
[6]梁开斌,马现涛.加减柴胡龙骨牡蛎汤队冠心病并发抑郁焦虑患者中医症候及负性心理的影响[J].中西医结合心脑血管病杂志,2017,5(31):137-138.
[7]Shi S,Liang J,Liu T,et al. Depression increases sympathetic activity and exacerbates myocardial remodeling after myocardial infarction:evidence from an animal experiment[J]. PLo S One,2014,9(7):e101734.
[8]Reese RL,Freedland KE,Steinmeyer BC,et al. Depression and rehospitalization following acute myocardial infarction[J]. Circ Cardiovasc Qual Outcomes,2011,4(6):626-633.
[9]黄佐,樊民,吴宗贵.冠心病患者抑郁症状与冠状动脉病变的关系[J].中华心血管病杂志,2005,9(4):329.
[10]Carney RM,Biumenthal JA,Freedland KE,et a1. Low heart rate variability and the effect of depression on post-myocardial infarction mortality[J]. Arch Intem Med,2005,165:1486.
[11]吴大兴.综合医院心理联络会诊[M].北京:人民卫生出版社,2011:131-137.
[12]Cooper GI. The safety of fluoxctine-anupdate[J]. Br J Psychiatry,2003,153:77-86
[13]包祖晓,管利民.柴胡加龙骨牡蛎汤在神经精神疾病中的运用[J].河北中医,2003,25(11):838-839.