藏药牦牛角有效成分的分离与解热镇痛物质基础的研究
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摘要
目的:寻找藏药材牦牛角中解热镇痛物质。方法:实验以牦牛角为原料,经回流提取得到粗提液,再经过透析和非变性电泳分离,得到不同分子量大小的多肽;采用皮下酵母注射法建立小鼠发热模型,热板法评价镇痛活性。结果:牦牛角回流粗提取液经透析、非变性电泳分离后,得到的小分子多肽在给药30min后能显著降低肛温,达到最大痛阈,并提高值到67%。结论:研究证实牦牛角粗提取液有明显的解热和镇痛作用,为进一步获得新的多肽类解热镇痛候选药物奠定基础。
Objective: Tibetan yak horns were selected to find the antipyretic and analgesic substances. Methods: As raw material,the yak horns were grinded to obtain a crude extract by reflux extraction,then they were treated by dialysis and non- denaturing gel electrophoresis separation,and the different molecular weight peptides were obtained; by using subcutaneous injection of yeast,the mouse fever model was established,and the hot plate method was established to evaluate the analgesic activity. Results: The small molecular peptides could significantly reduce rectal temperature,and get maximum pain threshold,and the value was increased to 67%. Conclusion: Our study confirmed the crude extracts from horns have significantly antipyretic and analgesic effects,and our finding is important for further getting a new class of anti- inflammatory candidate peptide drugs.
Objective: Tibetan yak horns were selected to find the antipyretic and analgesic substances. Methods: As raw material,the yak horns were grinded to obtain a crude extract by reflux extraction,then they were treated by dialysis and non- denaturing gel electrophoresis separation,and the different molecular weight peptides were obtained; by using subcutaneous injection of yeast,the mouse fever model was established,and the hot plate method was established to evaluate the analgesic activity. Results: The small molecular peptides could significantly reduce rectal temperature,and get maximum pain threshold,and the value was increased to 67%. Conclusion: Our study confirmed the crude extracts from horns have significantly antipyretic and analgesic effects,and our finding is important for further getting a new class of anti- inflammatory candidate peptide drugs.
引文
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[2]范伟强,李建华.试论藏药产业现代化发展的策略与出路[J].西北药学杂志,2007,22(1):46-48.
[3]帝玛尔·丹增彭措.晶珠本草[M].毛继祖,罗达尚,王振华,译.上海:上海科学技术出版社,1986:428-436.
[4]国家药典委员会.中华人民共和国药典(一部)[S].北京:化学工业出版社,2005:563-568.
[5]利毛才让,刘接卿,胡文祥.藏药现代研究进展[J].西北药学杂志,2007,22(6):350-352.
[6]宋风霜.牦牛角解热镇静安神[J].四川中医,2007,25(2):55-58.
[7]索有瑞,李天才.藏药牦牛角中的主要化学成分[J].中医药学报,2001,29(1):35-37.
[8]陈赤.水牛角的研究与应用[J].广西中医学院学报,2004,7(4):72-75.
[9]胡春萍,蒋加进,石磊,等.牦牛角等6种角类药超细粉对发热家兔的影响[J].医学动物防治,2004,22(4):235-238.
[10]于志海,刘学忠,李建基,等.牛胆汁中蛋白质种类及钙结合蛋白的电泳分析[J].扬州大学学报,2006,30(2):9-12.