β-隐黄素对大鼠实验性牙周炎炎症因子的影响
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摘要
目的:研究β-隐黄素对大鼠实验性牙周炎炎症因子的影响并探讨其相关作用机制。方法:30只雄性SD大鼠随机分为正常组(N组)、牙周炎组(P组)、β-隐黄素干预组(E组),每组10只。采取结扎双侧上颌第二磨牙颈部联合注射脂多糖(lipopolysaccharide,LPS)诱导牙周炎模型。E组同时于相同位点注射β-隐黄素(每只12μL),每48 h注射1次,共3次。实验第7天采血并立即分离双侧上颌骨,右侧颌骨行组织学分析,血清及左侧分离的牙龈组织以酶联免疫吸附试验(enzyme linked immunosorbent assay,ELISA)法检测细胞白介素-1β(interleukin-1β,IL-1β)、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、前列腺素E2(prostaglandin E2,PGE_2)含量。结果:E组和P组大鼠IL-1β、TNF-α、PGE_2水平明显高于N组,且炎症细胞浸润较多,牙槽骨吸收明显。与P组比较,E组中IL-1β、TNF-α、PGE_2水平明显降低(P<0.05),炎症细胞浸润较少,牙槽骨吸收减轻。结论:β-隐黄素可能通过降低组织中的炎症因子水平,减少炎症细胞浸润,从而减轻牙周组织的炎症反应,减少牙槽骨吸收。
Objective: To study the effect of β-cryptoxanthin on inflammatory factors in rats with experimental periodontitis and to explore its mechanism. Methods: 30 male Sprague-Dawley rats were randomly divided into normal group(group N), periodontitis group(group P), and β-cryptoxanthin intervention group(group E), with 10 rats of each. Experimental periodontitis model was induced by ligation of bilateral maxillary second molars cervix and injection with lipopolysaccharide(LPS). At the same time, β-cryptoxanthin(12μl/each) was injected in group E at the same site. All groups were treated every 48 hours for three times. Blood was collected and the bilateral maxilla were immediately separated on day 7, the right part of the tissues were subjected to histological analysis, serum and the left gingival tissues were detected by ELISA for IL-1β, TNF-α, and PGE_2. Results: The levels of IL-1β, TNF-α and PGE_2 in group E and group P were higher than those in group N, the infiltration of inflammatory cells was increased and the alveolar bone absorption was obvious.Compared with group P,the levels of IL-1β,TNF-α,and PGE2 in group E were significantly lower,the infiltration of inflammatory cells was less,and the absorption of alveolar bone was mild.Conclusion:β-cryptoxanthin can decrease the level of inflammatory factors and the inflammatory cells infiltration in tissues,thereby reducing the inflammation of periodontal tissues and alleviating alveolar bone resorption.
Objective: To study the effect of β-cryptoxanthin on inflammatory factors in rats with experimental periodontitis and to explore its mechanism. Methods: 30 male Sprague-Dawley rats were randomly divided into normal group(group N), periodontitis group(group P), and β-cryptoxanthin intervention group(group E), with 10 rats of each. Experimental periodontitis model was induced by ligation of bilateral maxillary second molars cervix and injection with lipopolysaccharide(LPS). At the same time, β-cryptoxanthin(12μl/each) was injected in group E at the same site. All groups were treated every 48 hours for three times. Blood was collected and the bilateral maxilla were immediately separated on day 7, the right part of the tissues were subjected to histological analysis, serum and the left gingival tissues were detected by ELISA for IL-1β, TNF-α, and PGE_2. Results: The levels of IL-1β, TNF-α and PGE_2 in group E and group P were higher than those in group N, the infiltration of inflammatory cells was increased and the alveolar bone absorption was obvious.Compared with group P,the levels of IL-1β,TNF-α,and PGE2 in group E were significantly lower,the infiltration of inflammatory cells was less,and the absorption of alveolar bone was mild.Conclusion:β-cryptoxanthin can decrease the level of inflammatory factors and the inflammatory cells infiltration in tissues,thereby reducing the inflammation of periodontal tissues and alleviating alveolar bone resorption.
引文
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[5] Moradi J,Abbasipour F,Zaringhalam J,et al.Anethole,a medicinal plant compound,decreases the production of pro-inflammatory TNF-alpha and IL-1β in a rat model of LPS-induced periodontitis [J].Iran J Pharm Res,2014,13(4):1319-1325.
[6] Matsumoto C,Ashida N,Yokoyama S,et al.The protective effects of β-cryptoxanthin on inflammatory bone resorption in a mouse experimental model of periodontitis [J].Biosci Biotechnol Biochem,2013,77(4):860-862.
[7] Cekici A,Kantarci A,Hasturk H,et al.Inflammatory and immune pathways in the pathogenesis of periodontal disease [J].Periodontol 2000,2014,64(1):57-80.
[8] 盛冉,孙志高,黄巧娟,等.β-隐黄素的生理活性及其机理研究进展[J].食品与机械,2016,32(12):218-223.
[9] 韩静,李威,李一梓,等.牙周炎伴COPD患者血清25-(OH)D、TNF-α和IL-6水平检测的研究[J].口腔医学研究,2014,30(8):743-746.
[10] 韩斐斐,郑纪伟,丁志江,等.冠心病与牙周病患者IL-6、TNF-a、PGE2水平相关性研究[J].口腔医学研究,2013,29(10):936-938.
[11] Linden GJ,McClean KM,Woodside JV,et al.Antioxidants and periodontitis in 60-70-year-old men [J].J Clin Periodontol,2009,36(10):843-849.
[2] Ciccone MM,Cortese F,Gesualdo M,et al.Dietary intake of carotenoids and their antioxidant and anti-inflammatory effects in cardiovascular care [J].Mediators Inflamm,2013,2013:782137.
[3] Nishigaki M,Yamamoto T,Ichioka H,et al.β-cryptoxanthin regulates bone resorption related-cytokine production in human periodontal ligament cells [J].Arch Oral Biol,2013,58(7):880-886.
[4] 轩亚茹,卫晓霞,崔晓宇,等.β-隐黄素对大鼠牙槽骨吸收的影响[J].安徽医科大学学报,2018,53(1):76-80.
[5] Moradi J,Abbasipour F,Zaringhalam J,et al.Anethole,a medicinal plant compound,decreases the production of pro-inflammatory TNF-alpha and IL-1β in a rat model of LPS-induced periodontitis [J].Iran J Pharm Res,2014,13(4):1319-1325.
[6] Matsumoto C,Ashida N,Yokoyama S,et al.The protective effects of β-cryptoxanthin on inflammatory bone resorption in a mouse experimental model of periodontitis [J].Biosci Biotechnol Biochem,2013,77(4):860-862.
[7] Cekici A,Kantarci A,Hasturk H,et al.Inflammatory and immune pathways in the pathogenesis of periodontal disease [J].Periodontol 2000,2014,64(1):57-80.
[8] 盛冉,孙志高,黄巧娟,等.β-隐黄素的生理活性及其机理研究进展[J].食品与机械,2016,32(12):218-223.
[9] 韩静,李威,李一梓,等.牙周炎伴COPD患者血清25-(OH)D、TNF-α和IL-6水平检测的研究[J].口腔医学研究,2014,30(8):743-746.
[10] 韩斐斐,郑纪伟,丁志江,等.冠心病与牙周病患者IL-6、TNF-a、PGE2水平相关性研究[J].口腔医学研究,2013,29(10):936-938.
[11] Linden GJ,McClean KM,Woodside JV,et al.Antioxidants and periodontitis in 60-70-year-old men [J].J Clin Periodontol,2009,36(10):843-849.