石墨炉原子吸收光谱法测定唾液中的锰
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摘要
[目的]建立一种唾液中锰的石墨炉原子吸收分光光度检测方法。[方法]选取接触锰的234名工人为接触组,再将其分为高浓度接触组和低浓度接触组;选取不接触锰的135名工人作为对照组。采集唾液,离心,取0.5 mL上清液加硝酸消解,用体积分数0.2%硝酸定容至2 mL后,于原子吸收仪上测定。[结果]方法最佳灰化温度1100℃,最佳原子化温度1900℃,一次线性回归方程为Y=0.01587 x+0.00480,相关系数0.9992(P<0.01),特征浓度5.4 pg/0.0044 Abs,检出限为5.8 pg,加标回收率为85%~106%,相对标准偏差1.5%~1.8%。对照组工人唾液锰质量浓度均值(7.65±5.57)μg/L,接触组工人(10.63±12.37)μg/L,两组差异有统计学意义(P<0.01)。低浓度接触组唾液锰质量浓度(7.68±6.98)μg/L,高浓度接触组为(13.38±15.36)μg/L,两组均值差异有统计学意义(P<0.01)。[结论]该方法下不同锰接触水平人员的唾液中锰检测值不同,且该方法灵敏度高,准确度好,操作简便,故提示其可能适合于人体中锰的采样测定。
[Objective] To establish the graphite furnace atomic absorption spectrometry method to examine the manganese in saliva. [Methods] After centrifuge of saliva,0.5 mL of the supernatant was digested with nitric acid. 0.2% nitric acid was added to the constant volume 2 mL. The specimen was measured with atomic absorption spectrometer. Then samples of 234 welding workers and 135 control workers were detected. [Results] The best ashing temperature of the method was 1100 ℃,and the optimum atomic temperature was 1900 ℃. The linear regression equation was Y = 0.01587 x + 0.00480 with the correlation coefficient 0.9992(P < 0.01). The characteristics of concentration was 5.4 pg/0.0044 Abs,and the detection limit was 5.8 pg.The recovery rate was 85%-106% with relative standard deviation 1.5%-1.8%. The average value of saliva manganese concentration was(7.65 ± 5.57) g/L and(10.63 ± 12.37) g/L in the control and welding workers,respectively(P < 0.01). The concentration of manganese varied among workers with lower or higher exposure level,(7.68 ± 6.98)g/L vs.(13.38 + 15.36)g/L(P < 0.01). [Conclusion] The method is highly sensitive and accurate,easy to manipulate and well repeated. These suggested the method may be suitable for sampling and determination of manganese in human body.
[Objective] To establish the graphite furnace atomic absorption spectrometry method to examine the manganese in saliva. [Methods] After centrifuge of saliva,0.5 mL of the supernatant was digested with nitric acid. 0.2% nitric acid was added to the constant volume 2 mL. The specimen was measured with atomic absorption spectrometer. Then samples of 234 welding workers and 135 control workers were detected. [Results] The best ashing temperature of the method was 1100 ℃,and the optimum atomic temperature was 1900 ℃. The linear regression equation was Y = 0.01587 x + 0.00480 with the correlation coefficient 0.9992(P < 0.01). The characteristics of concentration was 5.4 pg/0.0044 Abs,and the detection limit was 5.8 pg.The recovery rate was 85%-106% with relative standard deviation 1.5%-1.8%. The average value of saliva manganese concentration was(7.65 ± 5.57) g/L and(10.63 ± 12.37) g/L in the control and welding workers,respectively(P < 0.01). The concentration of manganese varied among workers with lower or higher exposure level,(7.68 ± 6.98)g/L vs.(13.38 + 15.36)g/L(P < 0.01). [Conclusion] The method is highly sensitive and accurate,easy to manipulate and well repeated. These suggested the method may be suitable for sampling and determination of manganese in human body.
引文
[1]金泰廙.职业卫生与职业医学[M].北京:人民卫生出版社,2007:93.
[2]杜旭芹,王涤新,牛立君,等.电焊工人唾液锰和血清锰及尿锰的改变[J].中华劳动卫生职业病杂志,2007,25(12):744-746.
[3]周远忠,陈健,史秀娟,等.人群锰接触水平的早期生物标志物探索[J].中华劳动卫生职业病杂志,2010,28(9):645-647.
[4]隋典朋,邓妍,许洁,等.锰暴露早期生物标志探索[J].遵义医学院学报,2011,34(6):583-587.
[5]史秀娟,邹焰,熊云刚,等.锰铁合金男性冶炼工人接触生物标志物的探索[J].工业卫生与职业病,2013,39(1):22-24.
[6]叶开友,徐瑞芳,顾春,等.职业性锰接触电焊工唾液锰和尿高香草酸水平的研究[J].环境与职业医学,2014,31(9):720-722.
[7]中华人民共和国卫生部.工作场所空气中锰及其化合物的测定方法:GBZ/T 160.13-2004[S].北京:人民卫生出版社,2004.
[2]杜旭芹,王涤新,牛立君,等.电焊工人唾液锰和血清锰及尿锰的改变[J].中华劳动卫生职业病杂志,2007,25(12):744-746.
[3]周远忠,陈健,史秀娟,等.人群锰接触水平的早期生物标志物探索[J].中华劳动卫生职业病杂志,2010,28(9):645-647.
[4]隋典朋,邓妍,许洁,等.锰暴露早期生物标志探索[J].遵义医学院学报,2011,34(6):583-587.
[5]史秀娟,邹焰,熊云刚,等.锰铁合金男性冶炼工人接触生物标志物的探索[J].工业卫生与职业病,2013,39(1):22-24.
[6]叶开友,徐瑞芳,顾春,等.职业性锰接触电焊工唾液锰和尿高香草酸水平的研究[J].环境与职业医学,2014,31(9):720-722.
[7]中华人民共和国卫生部.工作场所空气中锰及其化合物的测定方法:GBZ/T 160.13-2004[S].北京:人民卫生出版社,2004.