Ag@Parylene-C纳米结构制备技术研究及其SERS性能表征
|
摘要
针对二氧化硅包覆材料可重复利用性较差的问题,采用Parylene-C薄膜对银纳米颗粒进行包覆,制备了简易的微流体沟道,并与Ag@Parylene-C纳米结构衬底集成。通过表征Ag@Parylene-C纳米结构衬底对拉曼信号的增强效果及其在液相环境下的稳定性可得出:当沉积的Ag@Parylene-C纳米结构衬底膜厚为100nm时,其包覆效果较好且SERS活性较强,对结晶紫分子的检测限可达10~(-10)M。并且通过与微流体沟道集成,其可重复利用次数可以增加到500次,衰减幅度可减小到10%以下,充分证明了Ag@Parylene-C纳米结构SERS基底在微流体检测应用中具有广泛应用前景。
Parylene-C was then deposited on the Ag NPs substrate to deal with the poor reusability of the Ag@ SiO_2 substrate. A kind of simple microfluidic channel structure was fabricated and then integrated with the Ag@ Parylene-C substrate to test the SERS activity and stability of the substrate in the liquid phase environment. It is found that the Ag @ Parylene-C substrate with a thickness of 100 nm showed the fine coating effect and high SERS activity. After integrated with the microfluidic channel structure,the detecting limit of CV molecules that absorbed on Ag@ Parylene-C substrate could reach 10~(-10) M,and the reused times of the Ag @ Parylene-C substrate can be extended from 20 to 500. After the 500 testing experiments,the attenuation on SERS activity of the Ag @ Parylene-C substrate is less than 10%. The result indicated that the Ag@ Parylene-C substrate can be the candidate for the in-situ microfluidic SERS biochemical sensing application.
Parylene-C was then deposited on the Ag NPs substrate to deal with the poor reusability of the Ag@ SiO_2 substrate. A kind of simple microfluidic channel structure was fabricated and then integrated with the Ag@ Parylene-C substrate to test the SERS activity and stability of the substrate in the liquid phase environment. It is found that the Ag @ Parylene-C substrate with a thickness of 100 nm showed the fine coating effect and high SERS activity. After integrated with the microfluidic channel structure,the detecting limit of CV molecules that absorbed on Ag@ Parylene-C substrate could reach 10~(-10) M,and the reused times of the Ag @ Parylene-C substrate can be extended from 20 to 500. After the 500 testing experiments,the attenuation on SERS activity of the Ag @ Parylene-C substrate is less than 10%. The result indicated that the Ag@ Parylene-C substrate can be the candidate for the in-situ microfluidic SERS biochemical sensing application.
引文
[1] MCKIERNAN M,ZENG J,FERDOUS S,et al. Facile synthesis of bimetallic Ag/Ni core/sheath nanowires and their magnetic and electrical properties[J]. Small,2010,6(17):1927-34.
[2] CHEN L,ZHAO W,JIAO Y,et al. Characterization of Ag/Pt core-shell nanoparticles by UV-vis absorption,resonance light-scattering techniques[J]. Spectrochimica Acta Part A Molecular&Biomolecular Spectroscopy,2007,68(3):484-90.
[3] GAO C,LU Z,LIU Y,et al. Highly stable silver nanoplates for surface plasmon resonance biosensing[J]. Angewandte Chemie,2012,51(23):5629.
[4] GAO N,YANG T,LIU T,et al. Graphene Oxide Wrapped Individual Silver Nanocomposites with Improved Stability for Surface-enhanced Raman Scattering[J]. Rsc Advances,2015,5(69):55801-55807.
[5] ASLAN K,WU M,LAKOWICZ J R,et al. Fluorescent coreshell Ag@Si O2nanocomposites for metal-enhanced fluorescence and single nanoparticle sensing platforms[J]. Journal of the American Chemical Society,2007,129(6):1524.
[6] WU C,XU Q H. Stable and functionable mesoporous silicacoated gold nanorods as sensitive localized surface plasmon resonance(LSPR)nanosensors[J]. Langmuir,2009,25(16):9441.
[7] KONG X,YU Q,ZHANG X,et al. Synthesis and application of surface enhanced Raman scattering(SERS)tags of Ag@Si O2core/shell nanoparticles in protein detection[J]. Journal of Materials Chemistry,2012,22(16):7767-7774.
[8] LI X,LI J,ZHOU X,et al. Silver nanoparticles protected by monolayer graphene as a stabilized substrate for surface enhanced Raman spectroscopy[J]. Carbon,2014,66(2):713-719.
[9] CHEN P J,SHIH C Y,TAI Y C. Design,fabrication and characterization of monolithic embedded parylene microchannels in silicon substrate[J]. Lab on A Chip,2006,6(6):803.
[10] YASUDA H,CHUN B H,YANG D J,et al. Interface-Engineered Parylene C Coating for Corrosion Protection of ColdRolled Steel[J]. Corrosion-Houston Tx-,1996,52(52):169-176.
[11] VISAVELIYA N,LENKE S,KOEHLER J M. Composite Sensor Particles for Tuned SERS Sensing:Microfluidic Synthesis,Properties and Applications[J]. ACS Appl. Mater.Interfaces,2015,7(20):10742-10754.
[12] ZHANG X S,SU Z M,HAN M D,et al. Fabrication and characterization of the functional parylene-C film with micro/nano hierarchical structures[J]. Microelectronic Engineering,2015,141(15):72-80.
[2] CHEN L,ZHAO W,JIAO Y,et al. Characterization of Ag/Pt core-shell nanoparticles by UV-vis absorption,resonance light-scattering techniques[J]. Spectrochimica Acta Part A Molecular&Biomolecular Spectroscopy,2007,68(3):484-90.
[3] GAO C,LU Z,LIU Y,et al. Highly stable silver nanoplates for surface plasmon resonance biosensing[J]. Angewandte Chemie,2012,51(23):5629.
[4] GAO N,YANG T,LIU T,et al. Graphene Oxide Wrapped Individual Silver Nanocomposites with Improved Stability for Surface-enhanced Raman Scattering[J]. Rsc Advances,2015,5(69):55801-55807.
[5] ASLAN K,WU M,LAKOWICZ J R,et al. Fluorescent coreshell Ag@Si O2nanocomposites for metal-enhanced fluorescence and single nanoparticle sensing platforms[J]. Journal of the American Chemical Society,2007,129(6):1524.
[6] WU C,XU Q H. Stable and functionable mesoporous silicacoated gold nanorods as sensitive localized surface plasmon resonance(LSPR)nanosensors[J]. Langmuir,2009,25(16):9441.
[7] KONG X,YU Q,ZHANG X,et al. Synthesis and application of surface enhanced Raman scattering(SERS)tags of Ag@Si O2core/shell nanoparticles in protein detection[J]. Journal of Materials Chemistry,2012,22(16):7767-7774.
[8] LI X,LI J,ZHOU X,et al. Silver nanoparticles protected by monolayer graphene as a stabilized substrate for surface enhanced Raman spectroscopy[J]. Carbon,2014,66(2):713-719.
[9] CHEN P J,SHIH C Y,TAI Y C. Design,fabrication and characterization of monolithic embedded parylene microchannels in silicon substrate[J]. Lab on A Chip,2006,6(6):803.
[10] YASUDA H,CHUN B H,YANG D J,et al. Interface-Engineered Parylene C Coating for Corrosion Protection of ColdRolled Steel[J]. Corrosion-Houston Tx-,1996,52(52):169-176.
[11] VISAVELIYA N,LENKE S,KOEHLER J M. Composite Sensor Particles for Tuned SERS Sensing:Microfluidic Synthesis,Properties and Applications[J]. ACS Appl. Mater.Interfaces,2015,7(20):10742-10754.
[12] ZHANG X S,SU Z M,HAN M D,et al. Fabrication and characterization of the functional parylene-C film with micro/nano hierarchical structures[J]. Microelectronic Engineering,2015,141(15):72-80.