基于GC-MS技术的金钱草干预结晶肾损伤小鼠血清代谢组学研究
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摘要
目的利用血清代谢组学方法监测结晶肾损伤相关的代谢变化,评价金钱草提取物治疗结晶肾损伤的作用。方法将21只野生型C57BL/6小鼠随机分为3组:空白对照组、结晶肾损伤模型组和金钱草干预组。采用乙醛酸盐诱导小鼠结晶肾损伤模型,给予6 d相应的治疗后,取小鼠血清进行气相色谱-质谱联用仪分析,结合模式识别筛选模型组和对照组之间的差异代谢物,并以此评价金钱草的治疗作用。结果血清代谢组学分析鉴定了14种结晶肾损伤相关的生物标志物,包括嘌呤代谢物、氨基酸代谢物、脂肪酸代谢物等,同时数据显示金钱草提取物对结晶肾损伤有改善作用。结论金钱草提取物能够通过调节失衡的嘌呤代谢、氨基酸代谢、糖代谢等途径而发挥预防和治疗结晶肾损伤作用。
Objective To evaluate therapeutic effects of Lysimachiae Herba for crystallization kidney injury with serum metabolomics method.Methods 21 rats were randomly divided into three groups including control group,crystallization kidney injury model group and Lysimachiae Herba-treated group.The medicated group received Lysimachiae Herba for 6 days.Then,the serum samples of each rat were collected and analyzed by Gas Chromatography-Mass Spectrometer(GC-MS).The pattern recognition analysis of metabolomics differences among three groups was used to evaluate therapeutic effects of Lysimachiae Herba.Results 14 metabolites were identified through serum metabolomics analysis.The possible mechanism of crystallization kidney injury was mainly related to the metabolic products of purine,amino acid,fatty acid and sugar.The kidney injury by crystallization was alleviated by Lysimachiae Herba.Conclusion Lysimachiae Herba has preventive and therapeutic effects for crystallization kidney injury through partially regulating the perturbed purine metabolism,amino acid metabolism and glycometabolism.
Objective To evaluate therapeutic effects of Lysimachiae Herba for crystallization kidney injury with serum metabolomics method.Methods 21 rats were randomly divided into three groups including control group,crystallization kidney injury model group and Lysimachiae Herba-treated group.The medicated group received Lysimachiae Herba for 6 days.Then,the serum samples of each rat were collected and analyzed by Gas Chromatography-Mass Spectrometer(GC-MS).The pattern recognition analysis of metabolomics differences among three groups was used to evaluate therapeutic effects of Lysimachiae Herba.Results 14 metabolites were identified through serum metabolomics analysis.The possible mechanism of crystallization kidney injury was mainly related to the metabolic products of purine,amino acid,fatty acid and sugar.The kidney injury by crystallization was alleviated by Lysimachiae Herba.Conclusion Lysimachiae Herba has preventive and therapeutic effects for crystallization kidney injury through partially regulating the perturbed purine metabolism,amino acid metabolism and glycometabolism.
引文
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[2] 李昕.慢性肾功能衰竭并发症的研究进展[J].医学信息:中旬刊,2010,5(5):1334.
[3] 李飞艳,周日宝,刘群群.金钱草及其混伪品的微性状鉴定[J].海峡药学,2018,30(7):39-41.
[4] 武维波,刘永,刘桂营.利尿排石汤治疗泌尿系结石780例[J].实用中医药杂志,2013,29(7):559.
[5] 赵建一.金钱草治疗尿结石的机理作用研究[J].中医临床研究,2013,5(16):37-38.
[6] GUO L,TAN G G,LIU P,et al.Three plasma metabolite signatures for diagnosing high altitude pulmonary edema [J].Sci Rep,2015,5:15126.
[7] BIJLSMA S,BOBELDIJK I,VERHEIJ E R,et al.Large-scale human metabolomics studies:A strategy for data (pre-) processing and validation [J].Anal Chem,2006,78(2):567-574.
[8] EDVARDSSON V O,INDRIDASON O S,HARALDSSON G,et al.Temporal trends in the incidence of kidney stone disease [J].Kidney Int,2013,83(1):146-152.
[9] 彭明,顾向明,杜国有,等.泌尿系结石患者血清胱抑素C的含量变化分析[J].检验医学与临床,2011,8(2):172-173.
[10] ZAKARIA F A.Effect of oxygen derived free radicals and glycine on sodium-potassium adenosine triphosphatase in the basolateral membrane of the kidney in ischemia-reperfusion [J].Saudi Med J,2002,23(11):1380-1385.
[11] GARIBOTTO G,BONANNI A,VERZOLA D.Effect of kidney failure and hemodialysis on protein and amino acid metabolism [J].Curr Opin Clin Nutr Metab Care,2012,15(1):78-84.
[12] 张珺,尹磊,王昭君,等.脓毒症相关性肾损伤临床特征及预后分析[J].宁夏医学杂志,2018,40(9):807-809.