近红外特征谱段相关系数法测定减肥产品中的添加物酚酞
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摘要
目的建立近红外特征谱段相关系数法快速筛查减肥产品是否添加酚酞。方法采用近红外光谱仪的光纤探头采集光谱,以酚酞对照品的近红外光谱为参照光谱,运用导数、平滑等图谱处理技术,确定特征谱段、阈值等参数,建立酚酞检测模型。结果选定6065~5960 cm-1、4750~4650 cm-1为特征谱段,阈值设为30%,对35批样品进行检测,准确率达97%。结论该近红外特征谱段相关系数法模型可用于筛查减肥产品是否添加酚酞,速度快、成本低。
Objective To establish a near infrared spectroscopy for rapid detection of addition of phenolphthalein in weight loss product. Methods The spectra were collected using the fiber probe of a near infrared spectroradiometer.The reference spectra were set up using the spectra of certified phenolphthalein. All the spectra were preprocessed by derivative with smoothing points. The characteristic spectral band was chosen and the threshold was set to establish a model for the detection of phenolphthalein. Results The spectral ranges of 6065-5960 cm-1and 4750-4650 cm-1were chosen as the characteristic spectral bands, and 30% was set as the threshold. Totally 35 batches of samples were validated the model, and the accuracy was 97%. Conclusion The method is fast and inexpensive, which can be used in the detection of addition of phenolphthalein in weight loss product.
Objective To establish a near infrared spectroscopy for rapid detection of addition of phenolphthalein in weight loss product. Methods The spectra were collected using the fiber probe of a near infrared spectroradiometer.The reference spectra were set up using the spectra of certified phenolphthalein. All the spectra were preprocessed by derivative with smoothing points. The characteristic spectral band was chosen and the threshold was set to establish a model for the detection of phenolphthalein. Results The spectral ranges of 6065-5960 cm-1and 4750-4650 cm-1were chosen as the characteristic spectral bands, and 30% was set as the threshold. Totally 35 batches of samples were validated the model, and the accuracy was 97%. Conclusion The method is fast and inexpensive, which can be used in the detection of addition of phenolphthalein in weight loss product.
引文
[1]许凤,杨莹,李莉,等.TLC-FTIR联用技术检测减肥类保健食品中非法添加酚酞[J].卫生研究,2013,42(2):302-305.
[2]许凤,李莉,张宏莲,等.薄层色谱扫描法对减肥保健食品中酚酞的定性定量分析[J].药物分析杂志,2014,34(9):1634-1640.
[3]林芳,李涛,郭建博,等.UPLC-DAD法快速测定减肥类保健食品中违法添加的8种化学药物[J].食品科技,2014,39(7):318-323.
[4]黄奕滨,黄诺嘉,杨文红.HPLC法测定减肥类保健食品中非法添加酚酞的研究[J].齐鲁药事,2011,30(1):12-14.
[5]李静,李倚云,闻琍毓.HPLC法检测减肥类保健食品中非法添加的酚酞和盐酸西布曲明[J].天津药学,2015,27(2):17-18.
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[11]陆婉珍,袁洪福,徐光通,等.现代近红外光谱分析技术[M].北京:中国石化出版社,2000:1-13.
[12]黄必胜,袁明洋,余驰,等.基于近红外特征谱段相关系数法鉴别真伪龙齿[J].中国药师,2014,17(4):619-622.
[13]池文杰,张勋,陈海滨,等.近红外特征谱段相关系数法测定降压类中成药中添加氢氯噻嗪[J].药物分析杂志,2014,34(2):310-313.
[14]武永峰,董一威,胡新,等.基于近红外漫反射光谱的农田原位表层土壤含水量定量建模方法比较[J].光谱学与光谱分析,2015,35(12):3416-3421.
[15]涂瑶生,陈朋,孙冬梅,等.化橘红中柚皮苷含量近红外定量模型的建立[J].中药材,2014,37(9):1574-1578.
[16]李彬.盐酸坦洛新缓释胶囊近红外一致性检验模型的建立[J].中国药师,2014,17(12):2138-2142.
[17]陈伟华,许长华,樊玉霞,等.近红外光谱技术快速无损评价罗非鱼片新鲜度[J].食品科学,2014,35(24):164-168.
[18]库文娟,吕亚新.近红外光谱法快速鉴别脑心通胶囊的真伪[J].中国药事,2013,27(12):1291-1293.
[19]沈恒范.概率论与数理统计教程[M].北京:高等教育出版社,2003:108-109.
[20]王彩云,相秉仁,张伟,等.近红外光谱法快速检测牛奶中氯霉素残留[J].食品科学,2009,30(6):184-187.
[21]王学良,冯艳春,胡昌勤.近红外特征谱段相关系数法测定中药胶囊中添加枸橼酸西地那非[J].分析化学,2009,37(12):1825-1828.
[2]许凤,李莉,张宏莲,等.薄层色谱扫描法对减肥保健食品中酚酞的定性定量分析[J].药物分析杂志,2014,34(9):1634-1640.
[3]林芳,李涛,郭建博,等.UPLC-DAD法快速测定减肥类保健食品中违法添加的8种化学药物[J].食品科技,2014,39(7):318-323.
[4]黄奕滨,黄诺嘉,杨文红.HPLC法测定减肥类保健食品中非法添加酚酞的研究[J].齐鲁药事,2011,30(1):12-14.
[5]李静,李倚云,闻琍毓.HPLC法检测减肥类保健食品中非法添加的酚酞和盐酸西布曲明[J].天津药学,2015,27(2):17-18.
[6]谢敏.高效液相色谱法测定减肥类保健品中非法添加剂[J].广州化工,2015,43(4):150-152.
[7]张斌,姚家忠,贾昌平,等.减肥类保健食品中非法添加的8种化学药物的筛查与测定[J].中国执业药师,2015,12(3):22-27.
[8]王静文,黄湘鹭,曹进,等.超高效液相色谱法同时测定减肥类保健食品中非法添加的25种药物[J].色谱,2014,32(2):151-156.
[9]钮正睿,张庆生,曹进,等.超高效液相色谱-串联质谱法快速检测保健食品中非法添加的6种减肥类化学成分的研究[J].中国实验方剂学杂志,2014,20(18):91-94.
[10]郝刚,顾炳仁.UPLC-MS/MS法快速检测减肥类保健食品中5种非法添加化学成分[J].中国医药科学,2014,4(17):106-109.
[11]陆婉珍,袁洪福,徐光通,等.现代近红外光谱分析技术[M].北京:中国石化出版社,2000:1-13.
[12]黄必胜,袁明洋,余驰,等.基于近红外特征谱段相关系数法鉴别真伪龙齿[J].中国药师,2014,17(4):619-622.
[13]池文杰,张勋,陈海滨,等.近红外特征谱段相关系数法测定降压类中成药中添加氢氯噻嗪[J].药物分析杂志,2014,34(2):310-313.
[14]武永峰,董一威,胡新,等.基于近红外漫反射光谱的农田原位表层土壤含水量定量建模方法比较[J].光谱学与光谱分析,2015,35(12):3416-3421.
[15]涂瑶生,陈朋,孙冬梅,等.化橘红中柚皮苷含量近红外定量模型的建立[J].中药材,2014,37(9):1574-1578.
[16]李彬.盐酸坦洛新缓释胶囊近红外一致性检验模型的建立[J].中国药师,2014,17(12):2138-2142.
[17]陈伟华,许长华,樊玉霞,等.近红外光谱技术快速无损评价罗非鱼片新鲜度[J].食品科学,2014,35(24):164-168.
[18]库文娟,吕亚新.近红外光谱法快速鉴别脑心通胶囊的真伪[J].中国药事,2013,27(12):1291-1293.
[19]沈恒范.概率论与数理统计教程[M].北京:高等教育出版社,2003:108-109.
[20]王彩云,相秉仁,张伟,等.近红外光谱法快速检测牛奶中氯霉素残留[J].食品科学,2009,30(6):184-187.
[21]王学良,冯艳春,胡昌勤.近红外特征谱段相关系数法测定中药胶囊中添加枸橼酸西地那非[J].分析化学,2009,37(12):1825-1828.