Box-Behnken响应面法优化枇杷叶总多酚闪式提取工艺
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摘要
以总多酚得率为指标,在单因素实验基础上,采用Box-Behnken响应面法对影响枇杷叶总多酚提取得率的乙醇质量分数、提取时间、料液比等主要因素进行优化。优化的枇杷叶总多酚闪式提取工艺条件为:固定提取次数2次,采用32倍量质量分数62%乙醇溶液作为提取溶剂,闪式提取127 s,在此工艺条件下,枇杷叶总多酚得率为(33. 22±1. 6) mg·g~(-1),与模型预测值32. 86 mg·g~(-1)相近,并且远高于回流提取及超声提取条件下总多酚得率。响应面法优选的枇杷叶总多酚闪式提取工艺稳定,提取时间短、效率高,适合于工业化生产,实验结果对枇杷叶的规模化开发利用具有一定的参考价值。
With the extraction rate of total polyphenols as response value,the ethanol concentration,extraction time,liquid to material ratio were optimized by Box-Behnken response surface methodology( RSM) based on the results of single-factor experiment. The optimal conditions obtained by RSM were as follows: fixed extraction times was 2 times,ethanol concentration was 62%,liquid to material ratio was 32,extraction time was 127 s. Under these conditions,the experimental yield of total polyphenols was( 33. 22±1. 6) mg·g~(-1),which was well matched with the predictive yield of 32. 86 mg · g~(-1). Furthermore,flash extraction presented highest extraction rate compared with reflux extraction and ultrasonic extraction. The result revealed that flash extraction was simple,rapid and efficient,and the optimized extraction technology could provide the scientific basis for industrialized development.
With the extraction rate of total polyphenols as response value,the ethanol concentration,extraction time,liquid to material ratio were optimized by Box-Behnken response surface methodology( RSM) based on the results of single-factor experiment. The optimal conditions obtained by RSM were as follows: fixed extraction times was 2 times,ethanol concentration was 62%,liquid to material ratio was 32,extraction time was 127 s. Under these conditions,the experimental yield of total polyphenols was( 33. 22±1. 6) mg·g~(-1),which was well matched with the predictive yield of 32. 86 mg · g~(-1). Furthermore,flash extraction presented highest extraction rate compared with reflux extraction and ultrasonic extraction. The result revealed that flash extraction was simple,rapid and efficient,and the optimized extraction technology could provide the scientific basis for industrialized development.
引文
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[2]王建超,王卿,施文昊,等.响应面分析法优化枇杷叶多酚提取工艺[J].热带作物学报,2015,36(2):384-390.
[3]H Ito,E Kobayashi,S-H Li,et al.Antitumor activity of compounds isolated from the leaves of Eriobotrya japonica[J].Journal of Agricultural and Food Chemistry,2002,50(8):2400-2403.
[4]M Delfanian,R E Kenari,M A Sahari,et al.Evaluation of antioxidant activity of loquat fruit(Eriobotrya japonica lindl.)skin and the feasibility of their application to improve the oxidative stability of soybean oil[J].Journal of Food Science and Technology-Mysore,2016,53(5):2244-2252.
[5]P P K Zar,S Yano,K Sakao,et al.In vitro anticancer activity of loquat tea by inducing apoptosis in human leukemia cells[J].Bioscience Biotechnology and Biochemistry,2014,78(10):1731-1737.
[6]黄正虹.微波辅助提取枇杷叶中多酚的研究及工艺优化[J].食品工业,2014,35(11):193-196.
[7]丁建英,王晓飞,张丽.枇杷叶多酚超声波辅助提取工艺优化及其抗氧性分析[J].南方农业学报,2018,49(2):340-347.
[8]沈瑞.闪式提取在中药中的应用进展[J].中药材,2015,38(7):1540-1542.
[9]刘英,苏柘僮,陈龙,等.应用Box-Behnken设计优化地榆鞣质的闪式提取工艺[J].华西药学杂志,2012,27(3):304-306.
[10]赵宏,张宇,柴桂芳,等.沙棘多糖的闪式提取工艺条件的优化[J].辽宁中医杂志,2015,42(2):364-367.
[11]高虹,陈丽冰,周康,等.超声-闪式联合提取香菇柄麦角甾醇及其抗肿瘤活性[J].中国食品学报,2017,17(9):66-73.
[12]李康,王希希,崔晓瑞,等.红树莓多酚提取物闪式提取工艺优化及其抗氧化性[J].食品工业科技,2018,39(5):197-202.
[13]Xinsheng Wang,Yanfang Wu,Guangyun Chen,et al.Optimization of ultrasound assisted extraction of phenolic compoundsfrom Sparganii rhizoma with response surface methodology[J].Ultrasonics Sonochemistry,2013,20:846-854.
[14]刘英,张丽艳,罗君,等.单因素实验结合响应面法优化刺梨维生素C的提取工艺[J].辽宁中医杂志,2018,45(6):1236-1240.
[15]李精云,刘延泽.组织破碎提取法在中药研究中的应用进展[J].中草药,2011,42(10):2145-2149.