中药抗猪流行性腹泻及作用机制
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摘要
猪流行性腹泻(PED)主要侵害仔猪,发病率和病死率极高,严重影响养猪业的发展。目前,对于该病尚无有效治疗药物,细胞因子疗法、返饲等方法效果不确切。中药作为纯天然药物,含有多种有效活性成分,具有作用广泛、安全、低残留、低毒性、不易产生耐药性等优势,且已有研究证实许多单味中药及复方对猪流行性腹泻病毒(PEDV)均有抑制作用,己经成为当今最具发展前景的药物,被广泛用于PED的防控。论文就中药体外抑制PEDV的作用、临床应用,以及中药抑制或杀灭病毒,修复肠黏膜、减轻炎症反应,调控细胞干扰素信号通路,提高猪免疫力和肠内消化酶活性等作用机制进行综述。
Porcine epidemic diarrhea(PED)mainly affects piglets,and the incidence and mortality are extremely high,which seriously affects the development of the pig industry.At present,there is no effective treatment for the disease.Cytokine therapy,feeding back and other methods are not effective.As pure natural products,traditional Chinese medicines contain a variety of effective active ingredients.They have the advantages of wide-ranging effects,safe,low-residue,low-toxicity,and non easy producting drug resistance.It has been confirmed that many traditional Chinese medicines and compound prescriptions against porcine epidemic diarrhea virus(PEDV)have inhibitory effects and have become the most promising drugs and are widely used for the prevention and treatment of PED.This paper discussed the effects of traditional Chinese medicines on the inhibition of PEDVin vitro,clinical applications,and the mechanism of traditional Chinese medicines in suppressing or inactivating viruses,repairing intestinal mucosa,reducing inflammatory responses,regulating cell interferon signaling pathways,and improving pig immunity and intestinal digestive enzyme activities.
Porcine epidemic diarrhea(PED)mainly affects piglets,and the incidence and mortality are extremely high,which seriously affects the development of the pig industry.At present,there is no effective treatment for the disease.Cytokine therapy,feeding back and other methods are not effective.As pure natural products,traditional Chinese medicines contain a variety of effective active ingredients.They have the advantages of wide-ranging effects,safe,low-residue,low-toxicity,and non easy producting drug resistance.It has been confirmed that many traditional Chinese medicines and compound prescriptions against porcine epidemic diarrhea virus(PEDV)have inhibitory effects and have become the most promising drugs and are widely used for the prevention and treatment of PED.This paper discussed the effects of traditional Chinese medicines on the inhibition of PEDVin vitro,clinical applications,and the mechanism of traditional Chinese medicines in suppressing or inactivating viruses,repairing intestinal mucosa,reducing inflammatory responses,regulating cell interferon signaling pathways,and improving pig immunity and intestinal digestive enzyme activities.
引文
[1]夏伟,牟迪,唐志芬,等.2017年325个规模猪场7种病毒类疾病病原监测数据分析与2018年防控建议[J].养猪,2018(2):89-92.
[2]杨洪早,王东升,董书伟,等.仔猪腹泻的病因及中药防治研究进展[J].动物医学进展,2016,37(10):89-93.
[3]陈甜甜,程福亮,张建梅,等.大黄体外抗猪流行性腹泻病毒的研究[J].中国畜牧兽医,2015,42(9):2474-2480.
[4]朱买勋,余春成,朱兆荣,等.复方术芩提取液体外抗PEDV感染PK-15细胞的作用效果[J].中国兽医学报,2014,34(3):485-488.
[5]常洪涛,李永涛,黄慧敏,等.香连溶液阻断猪流行性腹泻病毒感染的临床效力研究[J].中国兽医杂志,2018,54(1):8-11.
[6] Huan C C,Wang H X,Sheng X X,et al.Glycyrrhizin inhibits porcine epidemic diarrhea virus infection and attenuates the proinflammatory responses by inhibition of high mobility group box-1protein[J].Arch Virol,2017,162(6):1467-1476.
[7] Lee J H,Park J S,Lee S W,et al.Porcine epidemic diarrhea virus infection:Inhibition by polysaccharide from Ginkgo biloba exocarp and mode of its action[J].Virus Res,2015,195(2):148-152.
[8]曾威,孙玉梅,马丰英,等.体外抗猪流行性腹泻病毒中药提取物的筛选[C]//中国畜牧兽医学会动物传染病学分会、解放军军事科学院军事医学研究院.中国畜牧兽医学会动物传染病学分会第九次全国会员代表大会暨第十七次全国学术研讨会论文集.贵阳:中国畜牧兽医学会动物传染病学分会、解放军军事科学院军事医学研究院,2017:60.
[9] Yang J L,Dhodary B,Ha T K Q,et al.Three new coumarins fromSaposhnikovia divaricata and their porcine epidemic diarrhea virus(PEDV)inhibitory activity[J].ChemInform,2015,71(28):4651-4658.
[10] Liu L L,Ha T K,Ha W,et al.Sesquiterpenoids with various carbocyclic skeletons from the flowers of Chrysanthemum indicum[J].J Nat Product,2017,80(2):298-307.
[11] Kim J W,Ha T K,Cho H,et al.Antiviral escin derivatives from the seeds of Aesculus turbinata Blume(Japanese horse chestnut)[J].Bioorganic Med Chem Let,2017,27(13):3019-3025.
[12]杨云乔,郑建高,姜军华,等.复方中药对猪流行性腹泻的治疗效果[J].中国兽医学报,2017,37(7):1353-1358.
[13]刘衍芬,李艳飞,鄂禄祥.中药复方对人工感染猪流行性腹泻病毒仔猪的治疗效果研究[J].黑龙江畜牧兽医,2016(18):155-158.
[14]尹宝英.自拟中药复方治疗猪流行性腹泻效果研究[J].黑龙江畜牧兽医,2014(22):90-100.
[15] Kim H B,Lee C Y,Kim S J,et al.Medicinal herb extracts ameliorate impaired growth performance and intestinal lesion of newborn piglets challenged with the virulent porcine epidemic diarrhea virus[J].J Anim Sci Technol,2015,57(1):33-39.
[16]陈亚乔,侯林,崔清华,等.中药抗病毒活性及作用机制研究进展[J].中医药导报,2017,23(22):103-106.
[17]孟冬霞,曹日亮,胡广英,等.超微粉中药对保育猪小肠形态发育的影响[J].中兽医医药杂志,2017,36(3):5-7.
[18] Keimer B,Kr9ger S,R9he I,et al.Influence of differently processed yeast(Kluyveromyces fragilis)on feed intake and gut physiology in weaned pigs[J].J Anim Sci,2018,96(1):194-205.
[19]杨云乔,郑建高,姜军华,等.复方中药治疗流行性腹泻病毒感染猪的消化道黏膜电镜观察及相关酶检测[J].江苏农业科学,2017,45(17):159-163.
[20] Guo L,Luo X,Li R,et al.Porcine epidemic diarrhea virus infection inhibits interferon signaling by targeted degradation of STAT1[J].J Virol,2016,90(18):8281-8292.
[21]周悦芳,范培红.中药免疫调节作用研究进展[J].时珍国医国药,2017,28(1):205-207.
[22]段明房,胡红伟,闫凌鹏,等.中草药益生菌复合制剂对生长猪血液常规、血清生化和血清免疫指标的影响[J].中国饲料,2018(3):54-59.
[23] Asha M K,Debraj D,Dethe S,et al.Effect of flavonoid-rich extract of Glycyrrhiza glabra on gut-friendly microorganisms,commercial probiotic preparations,and digestive enzymes[J].J Diet Suppl,2017,14(3):323-333.
[24]邢玉娟,高亚军,秦俊杰,等.苦参止痢颗粒的药效作用研究[J].动物医学进展,2017,38(12):91-94.
[25]文艳巧,姚万玲,杨朝雪,等.郁金散对大肠湿热证模型大鼠血清及肠道组织胃肠激素的影响[J].畜牧兽医学报,2017,48(6):1140-1149.
[26] Aleem A,Janbaz K H.Dual mechanisms of anti-muscarinic and Ca++antagonistic activities to validate the folkloric uses of Cyperus niveus Retz.as antispasmodic and antidiarrheal[J].J Ethnopharmacol,2018,213(3):138-148.
[27]侯博,曾建国.血根碱的生物学活性及博落回提取物在动物生产中的应用[J].动物营养学报,2018,30(2):413-420.
[28] Sándor Z,Mottaghipisheh J,Veres K,et al.Evidence supports tradition:The in vitro effects of Roman chamomile on smooth muscles[J].Front Pharmacol,2018,9(4):261-271.
[2]杨洪早,王东升,董书伟,等.仔猪腹泻的病因及中药防治研究进展[J].动物医学进展,2016,37(10):89-93.
[3]陈甜甜,程福亮,张建梅,等.大黄体外抗猪流行性腹泻病毒的研究[J].中国畜牧兽医,2015,42(9):2474-2480.
[4]朱买勋,余春成,朱兆荣,等.复方术芩提取液体外抗PEDV感染PK-15细胞的作用效果[J].中国兽医学报,2014,34(3):485-488.
[5]常洪涛,李永涛,黄慧敏,等.香连溶液阻断猪流行性腹泻病毒感染的临床效力研究[J].中国兽医杂志,2018,54(1):8-11.
[6] Huan C C,Wang H X,Sheng X X,et al.Glycyrrhizin inhibits porcine epidemic diarrhea virus infection and attenuates the proinflammatory responses by inhibition of high mobility group box-1protein[J].Arch Virol,2017,162(6):1467-1476.
[7] Lee J H,Park J S,Lee S W,et al.Porcine epidemic diarrhea virus infection:Inhibition by polysaccharide from Ginkgo biloba exocarp and mode of its action[J].Virus Res,2015,195(2):148-152.
[8]曾威,孙玉梅,马丰英,等.体外抗猪流行性腹泻病毒中药提取物的筛选[C]//中国畜牧兽医学会动物传染病学分会、解放军军事科学院军事医学研究院.中国畜牧兽医学会动物传染病学分会第九次全国会员代表大会暨第十七次全国学术研讨会论文集.贵阳:中国畜牧兽医学会动物传染病学分会、解放军军事科学院军事医学研究院,2017:60.
[9] Yang J L,Dhodary B,Ha T K Q,et al.Three new coumarins fromSaposhnikovia divaricata and their porcine epidemic diarrhea virus(PEDV)inhibitory activity[J].ChemInform,2015,71(28):4651-4658.
[10] Liu L L,Ha T K,Ha W,et al.Sesquiterpenoids with various carbocyclic skeletons from the flowers of Chrysanthemum indicum[J].J Nat Product,2017,80(2):298-307.
[11] Kim J W,Ha T K,Cho H,et al.Antiviral escin derivatives from the seeds of Aesculus turbinata Blume(Japanese horse chestnut)[J].Bioorganic Med Chem Let,2017,27(13):3019-3025.
[12]杨云乔,郑建高,姜军华,等.复方中药对猪流行性腹泻的治疗效果[J].中国兽医学报,2017,37(7):1353-1358.
[13]刘衍芬,李艳飞,鄂禄祥.中药复方对人工感染猪流行性腹泻病毒仔猪的治疗效果研究[J].黑龙江畜牧兽医,2016(18):155-158.
[14]尹宝英.自拟中药复方治疗猪流行性腹泻效果研究[J].黑龙江畜牧兽医,2014(22):90-100.
[15] Kim H B,Lee C Y,Kim S J,et al.Medicinal herb extracts ameliorate impaired growth performance and intestinal lesion of newborn piglets challenged with the virulent porcine epidemic diarrhea virus[J].J Anim Sci Technol,2015,57(1):33-39.
[16]陈亚乔,侯林,崔清华,等.中药抗病毒活性及作用机制研究进展[J].中医药导报,2017,23(22):103-106.
[17]孟冬霞,曹日亮,胡广英,等.超微粉中药对保育猪小肠形态发育的影响[J].中兽医医药杂志,2017,36(3):5-7.
[18] Keimer B,Kr9ger S,R9he I,et al.Influence of differently processed yeast(Kluyveromyces fragilis)on feed intake and gut physiology in weaned pigs[J].J Anim Sci,2018,96(1):194-205.
[19]杨云乔,郑建高,姜军华,等.复方中药治疗流行性腹泻病毒感染猪的消化道黏膜电镜观察及相关酶检测[J].江苏农业科学,2017,45(17):159-163.
[20] Guo L,Luo X,Li R,et al.Porcine epidemic diarrhea virus infection inhibits interferon signaling by targeted degradation of STAT1[J].J Virol,2016,90(18):8281-8292.
[21]周悦芳,范培红.中药免疫调节作用研究进展[J].时珍国医国药,2017,28(1):205-207.
[22]段明房,胡红伟,闫凌鹏,等.中草药益生菌复合制剂对生长猪血液常规、血清生化和血清免疫指标的影响[J].中国饲料,2018(3):54-59.
[23] Asha M K,Debraj D,Dethe S,et al.Effect of flavonoid-rich extract of Glycyrrhiza glabra on gut-friendly microorganisms,commercial probiotic preparations,and digestive enzymes[J].J Diet Suppl,2017,14(3):323-333.
[24]邢玉娟,高亚军,秦俊杰,等.苦参止痢颗粒的药效作用研究[J].动物医学进展,2017,38(12):91-94.
[25]文艳巧,姚万玲,杨朝雪,等.郁金散对大肠湿热证模型大鼠血清及肠道组织胃肠激素的影响[J].畜牧兽医学报,2017,48(6):1140-1149.
[26] Aleem A,Janbaz K H.Dual mechanisms of anti-muscarinic and Ca++antagonistic activities to validate the folkloric uses of Cyperus niveus Retz.as antispasmodic and antidiarrheal[J].J Ethnopharmacol,2018,213(3):138-148.
[27]侯博,曾建国.血根碱的生物学活性及博落回提取物在动物生产中的应用[J].动物营养学报,2018,30(2):413-420.
[28] Sándor Z,Mottaghipisheh J,Veres K,et al.Evidence supports tradition:The in vitro effects of Roman chamomile on smooth muscles[J].Front Pharmacol,2018,9(4):261-271.