摘要
2018年3月,河南焦作市某猪场发生疑似猪流行性腹泻(PED)感染,而该猪场进行过PED疫苗免疫,为确诊病原,采集患病仔猪肠管内容物,利用RT-PCR法,扩增猪流行性腹泻病毒(PEDV)部分S基因片段,并将产物送基因公司测序。所获测序结果,运用DNAStar和MEGA5软件,与GeneBank下载的不同毒株S基因片段进行同源性分析。结果显示:RT-PCR扩增产物与预期大小一致,为969bp;该毒株部分S基因与经典毒株CV777同源性为94.8%,亲缘关系较远;与近年来流行毒株同源性为98.1%~99.1%,亲缘关系较近。PEDV变异毒株的出现可能是导致免疫失败的重要原因。
In March 2018,a suspected Porcine Epidemic Diarrhea(PED) infection occurred in a pig farm in Jiaozuo,Henan Province.After our investigation,the pigs have been immunized with PED vaccine.To diagnose the cause of the infection,the intestinal contents of infected piglets was collected and RT-PCR was used to amplify the S gene segment of porcine epidemic diarrhea virus(PEDV), then the amplified S gene segment was sent to the gene company for sequencing.The sequencing result was obtained,The DNAStar and MEGA5 software were used to perform homology analysis with the S gene fragment of different strains downloaded from GeneBank.The results showed that the RT-PCR amplification product had the expected size of 969 bp;The homology of the S gene to the classical strain CV777 was 94.8% and the genetic relationship was far away;It has a homology of 98.1% to 99.1% with the prevalent strains in recent years and has a close genetic relationship.The emergence of PEDV variant strains may be the root cause of immune failure.
引文
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