‘鲁赫’与野生刺蔷薇花精油成分比较
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摘要
本研究提取‘鲁赫’刺蔷薇花和野生刺蔷薇花精油并测定其精油得率,比较其挥发性成分和脂肪酸组成。结果表明:‘鲁赫’刺蔷薇花精油得率明显高于野生刺蔷薇花(p<0.05);采用气相色谱-质谱联用技术对两种刺蔷薇花挥发性成分进行分离鉴定,经分析可以明确‘鲁赫’刺蔷薇花中含有已知成分70种,野生品种中的68种成分。主要挥发性成分为二十烷、二十五烷、三十烷和邻苯二甲酸二丁酯四种,经分析得挥发性成分组成及含量无明显差异(p>0.05);气相色谱法测定脂肪酸组成及含量,‘鲁赫’与野生刺蔷薇花均检测出29种脂肪酸成分,其中癸酸、棕榈油酸、反式油酸、二十碳酸含量较为丰富。‘鲁赫’刺蔷薇花作为改良品种与传统野生刺蔷薇花相比保留了野生刺蔷薇花原有特性。
In this study, the essential oils of the Rosa acicularis ‘Luhe’ and Lindl were measured and their oil yield, the volatile components and fatty acid composition were compared. The results showed that the oil yield of ‘Luhe’ was significantly higher than that of Lindl(p<0.05). With gas chromatography-mass spectrometry(GC-MS), we have separated and identified the volatile components of two kinds of Rosa acicularis. After analysis, it was clear that the ‘Luhe’ contains 70 kinds of known ingredients and Lindl contains 68 components. The main volatile components were eicosane, pentadecane, triacontane and dibutyl phthalate. There was no significant difference in volatile components content among Rosa acicularis species(p>0.05).The composition and content of fatty acids were determined by gas chromatography(GC), and 29 fatty acids were detected in both ‘Luhe’ and Lindl. Among them, decanoic acid, palm oleic acid, trans oleic acid,and twenty carbonic acid were abundant. Compared to traditional Rosa acicularis Lindl, the Rosa acicularis ‘Luhe’, as an improved variety,retains the original characteristics of wild species(Rosa acicularis Lindl).
In this study, the essential oils of the Rosa acicularis ‘Luhe’ and Lindl were measured and their oil yield, the volatile components and fatty acid composition were compared. The results showed that the oil yield of ‘Luhe’ was significantly higher than that of Lindl(p<0.05). With gas chromatography-mass spectrometry(GC-MS), we have separated and identified the volatile components of two kinds of Rosa acicularis. After analysis, it was clear that the ‘Luhe’ contains 70 kinds of known ingredients and Lindl contains 68 components. The main volatile components were eicosane, pentadecane, triacontane and dibutyl phthalate. There was no significant difference in volatile components content among Rosa acicularis species(p>0.05).The composition and content of fatty acids were determined by gas chromatography(GC), and 29 fatty acids were detected in both ‘Luhe’ and Lindl. Among them, decanoic acid, palm oleic acid, trans oleic acid,and twenty carbonic acid were abundant. Compared to traditional Rosa acicularis Lindl, the Rosa acicularis ‘Luhe’, as an improved variety,retains the original characteristics of wild species(Rosa acicularis Lindl).
引文
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[5]其买古丽·阿沙木,米丽班·霍加艾合买提,阿吾提·艾买尔,等.野蔷薇果皮多酚的提取与抗氧化和抑菌作用研究[J].华中师范大学学报(自然科学版),2014,48(6):850-856QIMANGULI Asha-um,MILIBAN Hoja-aihemaiti,AWUTIAi-maier,et al.Extraction of Polyphenols from Rosa multiflora Thunb and the study on their antioxidant and antibacterial activity[J].Journal of Huazhong Normal University(natural sciences),2014,48(6):850-856
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[7]Arthur H,Joubert E,Beer D D,et al.Phenylethanoid Glycosides as major antioxidants in Lippia multiflora,herbal infusion and their stability during steam pasteurisation of plant material[J].Food Chemistry,2011,127(2):581
[8]Zangiabadi M,Sahari M A,Barzegar M,et al.Zataria multiflora and Bunium persicum essential oils as two natural antioxidants[J].Chemistry,2012,12(26):6776-6786
[9]Harnafi H,Bouanani N H,Aziz M,et al.The hypolipidaemic activity of aqueous Erica multiflora flowers extract in triton WR-1339 induced hyperlipidaemic rats:A comparison with fenofibrate[J].Journal of Ethnopharmacology,2007,109(1):156-160
[10]Rouzbehan S,Moein S,Homaei A,et al.Kinetics ofα-glucosidase inhibition by different fractions of three species of Labiatae extracts:A new diabetes treatment model[J].Pharmaceutical Biology,2017,5(4):1483-1488
[11]Jeon H,Dong H K,Nho Y H,et al.A mixture of extracts of Kochia scoparia and Rosa multiflora with PPAR dual agonistic effects prevents photoaging in hairless Mice[J].International Journal of Molecular Sciences,2016,17(11):1919
[12]易照勤,初振欢,王昊天,等.‘鲁赫’刺蔷薇播种繁殖技术研究[J].高师理科学刊,2017,37(4):47-51YI Zhao-qin,CHU Zhen-huan,WANG Hao-tian,et al.Study on seed propagation technique of Rosa acicularis‘Luhe’[J].Journal of Science of Normal University,2017,37(4):47-51
[13]孙雨安,杜瑞,李振兴,等.基于顶空固相微萃取GC-MS的玫瑰花挥发性成分分析[J].河南农业大学学报,2014,48(4):461-464SUN Yu-an,DU Rui,LI Zhen-xing,et al.Determination of volatile components of Roses based on headspace-solid phase microextraction and GC-MS[J].Journal of Henan Agricultural University,2014,48(4):461-464
[14]Sadeghi H,Robati Z,Saharkhiz M J.Variability in Zataria multiflora,Bioss.essential oil of twelve populations from Fars province,Iran[J].Industrial Crops&Products,2015,67(April):221-226
[15]吴克刚,崔绮嫦,董艳,等.芳香植物精油对食品常见腐败微生物抗菌活性的研究[J].现代食品科技,2017,33(4):120-127,81WU Ke-gang,CUI Qi-chang,DONG Yan,et al.Antimicrobial effects of edible plant essential oils on common foodspoilage microorganisms[J].Modern Food Science and Technology,2017,33(4):120-127,81
[16]王卉,张明凯,胡锐.精油在食品抗菌包装中的应用研究进展[J].食品工业,2015,36(7):219-222WANG Hui,ZHANG Ming-kai,HU Rui.Application research development of plant essential oil in food antibacterial packaging[J].Food Industry,2015,36(7):219-222
[17]刘祥萍,黄薇,吴秀兰,等.高效液相色谱法测定化妆品中邻苯二甲酸二丁酯和邻苯二甲酸二(2-乙基己基)酯的含量[J].中国卫生检验杂志,2007,17(9):1563-1564,1648LIU Xiang-ping,HUANG Wei,WU Xiu-lan,et al.Determ Ination of dibutyl phthalate and bis(2-ethylhexyl)phthalate in cosmetics by high performance liquid chromatography[J].Chinese Journal of Health Laboratory Technology,2007,17(9):1563-1564,1648
[18]Hauser R,Calafat A M.Phthalates and Human health[J].Occupational&Environmental Medicine,2005,62(11):806-818
[19]赵秀英,张振杰,张宏利,等.黄蔷薇花精油化学成分的研究[J].西北植物学报,1994,14(5):154-156ZHAO Xiu-ying,ZHANG Zhen-jie,ZHANG Hong-li,et al.Study on chemical compounds of the essential oil from flowers of Rosa hugonis Hemsl[J].Acta Botanica Boreali-occidentalia Sinica,1994,14(5):154-156
[20]杨芳,禚苏,倪星,等.七里香蔷薇精油的化学成分分析[J].湖北农业科学,2016,55(10):2635-2636ZHANG Fang,ZHUO Su,NI Xing,et al.Chemical composition analysis of Rosa banksiae R.Br.var.noroalis Regel.plant essential oil[J].Hubei Agricultural Sciences,2016,55(10):2635-2636
[21]周学森,蒋玉梅,毕阳,等.苦水玫瑰精油提取及其成分的GC/MS分析[J].食品工业科技,2009,30(11):226-229ZHOU Xue-sen,JIANG Yu-mei,BI Yang,et al.Extraction of essential oil from Ku-shui Rose and component analysis by GC/MS[J].Science and Technology of Food Industry,2009,30(11):226-229
[22]Du H,Zhang X,Zhang R,et al.Extraction and the fatty acid profile of Rosa acicularis seed oil[J].Journal of Oleo Science,2017,66(12):1301-1310
[23]李双.牡丹花精油的提取、分析及抗氧化性研究[D].济南:齐鲁工业大学,2015LI Shuang.The extraction,analysis and antioxidant research in Peony essential Oil[D].Jinan:Qilu University of Technology,2015
[24]饶建平,王文成,张远志,等.水蒸气蒸馏法提取柚子花精油工艺研究及其成分分析[J].食品工业科技,2017,38(4):278-282,299RAO Jian-ping,WANG Wen-cheng,ZHANG Yuan-zhi,et al.Study on the extracting process of essential oil from Citrus maxima flower by steam distillationand the analysis for the composition of the essential oil[J].Science and Technology of Food Industry,2017,38(4):278-282,299
[25]丁成斌,熊光同,王强.毛叶蔷薇花净油化学成分的研究[J].香料香精化妆品,1991,1:1-4DING Cheng-bin,XIONG Guang-tong,WANG Qiang.Study on chemical compounds of the aromatic from petals of Rosa mairei Levl[J].Essential Spices Cosmetics,1991,1:1-4
[2]阮立为,冷雪尘,陈桂英.蔷薇属灌木的主要化学成分及综合利用[J].中国林副特产,1994,3:41-42RUAN Li-wei,LENG Xue-chen,CHEN Gui-ying.Main chemical constituents and comprehensive utilization of Rosacea shrubs[J].Chinese Forest Products,1994,3:41-42
[3]赵永春.蔷薇花验方[N].中国中医药报,2010-05-06(005)ZHAO Yong-chun.Rose Prescription[N].China news of traditional Chinese medicine,2010-05-06(005)
[4]Azizkhani M,Elizaquível P,Sánchez G,et al.Comparative efficacy of Zataria multiflora Boiss.Origanum compactum and Eugenia caryophyllus essential oils against E.coli O157:H7,feline calicivirus and endogenous microbiota in commercial baby-leaf salads[J].International Journal of Food Microbiology,2013,166(2):249-255
[5]其买古丽·阿沙木,米丽班·霍加艾合买提,阿吾提·艾买尔,等.野蔷薇果皮多酚的提取与抗氧化和抑菌作用研究[J].华中师范大学学报(自然科学版),2014,48(6):850-856QIMANGULI Asha-um,MILIBAN Hoja-aihemaiti,AWUTIAi-maier,et al.Extraction of Polyphenols from Rosa multiflora Thunb and the study on their antioxidant and antibacterial activity[J].Journal of Huazhong Normal University(natural sciences),2014,48(6):850-856
[6]阿吾提·艾买尔,古力齐曼·阿布力孜,其买古丽·阿沙木,等.响应曲面法优化野蔷薇根多糖的提取工艺及抑菌作用研究[J].新疆师范大学学报(自然科学版),2016,35(2):10-17AWUTI AIMAIER,Gulqiman·ABLIZ,Qimanguli·ASHAMU,et al.Response surface methodology for optimization of extraction conditions of polysaccharide from Rosa multiflora Thunb and antibacterial activity[J].Journal of Xinjiang Normal University(Natural Sciences),2016,35(2):10-17
[7]Arthur H,Joubert E,Beer D D,et al.Phenylethanoid Glycosides as major antioxidants in Lippia multiflora,herbal infusion and their stability during steam pasteurisation of plant material[J].Food Chemistry,2011,127(2):581
[8]Zangiabadi M,Sahari M A,Barzegar M,et al.Zataria multiflora and Bunium persicum essential oils as two natural antioxidants[J].Chemistry,2012,12(26):6776-6786
[9]Harnafi H,Bouanani N H,Aziz M,et al.The hypolipidaemic activity of aqueous Erica multiflora flowers extract in triton WR-1339 induced hyperlipidaemic rats:A comparison with fenofibrate[J].Journal of Ethnopharmacology,2007,109(1):156-160
[10]Rouzbehan S,Moein S,Homaei A,et al.Kinetics ofα-glucosidase inhibition by different fractions of three species of Labiatae extracts:A new diabetes treatment model[J].Pharmaceutical Biology,2017,5(4):1483-1488
[11]Jeon H,Dong H K,Nho Y H,et al.A mixture of extracts of Kochia scoparia and Rosa multiflora with PPAR dual agonistic effects prevents photoaging in hairless Mice[J].International Journal of Molecular Sciences,2016,17(11):1919
[12]易照勤,初振欢,王昊天,等.‘鲁赫’刺蔷薇播种繁殖技术研究[J].高师理科学刊,2017,37(4):47-51YI Zhao-qin,CHU Zhen-huan,WANG Hao-tian,et al.Study on seed propagation technique of Rosa acicularis‘Luhe’[J].Journal of Science of Normal University,2017,37(4):47-51
[13]孙雨安,杜瑞,李振兴,等.基于顶空固相微萃取GC-MS的玫瑰花挥发性成分分析[J].河南农业大学学报,2014,48(4):461-464SUN Yu-an,DU Rui,LI Zhen-xing,et al.Determination of volatile components of Roses based on headspace-solid phase microextraction and GC-MS[J].Journal of Henan Agricultural University,2014,48(4):461-464
[14]Sadeghi H,Robati Z,Saharkhiz M J.Variability in Zataria multiflora,Bioss.essential oil of twelve populations from Fars province,Iran[J].Industrial Crops&Products,2015,67(April):221-226
[15]吴克刚,崔绮嫦,董艳,等.芳香植物精油对食品常见腐败微生物抗菌活性的研究[J].现代食品科技,2017,33(4):120-127,81WU Ke-gang,CUI Qi-chang,DONG Yan,et al.Antimicrobial effects of edible plant essential oils on common foodspoilage microorganisms[J].Modern Food Science and Technology,2017,33(4):120-127,81
[16]王卉,张明凯,胡锐.精油在食品抗菌包装中的应用研究进展[J].食品工业,2015,36(7):219-222WANG Hui,ZHANG Ming-kai,HU Rui.Application research development of plant essential oil in food antibacterial packaging[J].Food Industry,2015,36(7):219-222
[17]刘祥萍,黄薇,吴秀兰,等.高效液相色谱法测定化妆品中邻苯二甲酸二丁酯和邻苯二甲酸二(2-乙基己基)酯的含量[J].中国卫生检验杂志,2007,17(9):1563-1564,1648LIU Xiang-ping,HUANG Wei,WU Xiu-lan,et al.Determ Ination of dibutyl phthalate and bis(2-ethylhexyl)phthalate in cosmetics by high performance liquid chromatography[J].Chinese Journal of Health Laboratory Technology,2007,17(9):1563-1564,1648
[18]Hauser R,Calafat A M.Phthalates and Human health[J].Occupational&Environmental Medicine,2005,62(11):806-818
[19]赵秀英,张振杰,张宏利,等.黄蔷薇花精油化学成分的研究[J].西北植物学报,1994,14(5):154-156ZHAO Xiu-ying,ZHANG Zhen-jie,ZHANG Hong-li,et al.Study on chemical compounds of the essential oil from flowers of Rosa hugonis Hemsl[J].Acta Botanica Boreali-occidentalia Sinica,1994,14(5):154-156
[20]杨芳,禚苏,倪星,等.七里香蔷薇精油的化学成分分析[J].湖北农业科学,2016,55(10):2635-2636ZHANG Fang,ZHUO Su,NI Xing,et al.Chemical composition analysis of Rosa banksiae R.Br.var.noroalis Regel.plant essential oil[J].Hubei Agricultural Sciences,2016,55(10):2635-2636
[21]周学森,蒋玉梅,毕阳,等.苦水玫瑰精油提取及其成分的GC/MS分析[J].食品工业科技,2009,30(11):226-229ZHOU Xue-sen,JIANG Yu-mei,BI Yang,et al.Extraction of essential oil from Ku-shui Rose and component analysis by GC/MS[J].Science and Technology of Food Industry,2009,30(11):226-229
[22]Du H,Zhang X,Zhang R,et al.Extraction and the fatty acid profile of Rosa acicularis seed oil[J].Journal of Oleo Science,2017,66(12):1301-1310
[23]李双.牡丹花精油的提取、分析及抗氧化性研究[D].济南:齐鲁工业大学,2015LI Shuang.The extraction,analysis and antioxidant research in Peony essential Oil[D].Jinan:Qilu University of Technology,2015
[24]饶建平,王文成,张远志,等.水蒸气蒸馏法提取柚子花精油工艺研究及其成分分析[J].食品工业科技,2017,38(4):278-282,299RAO Jian-ping,WANG Wen-cheng,ZHANG Yuan-zhi,et al.Study on the extracting process of essential oil from Citrus maxima flower by steam distillationand the analysis for the composition of the essential oil[J].Science and Technology of Food Industry,2017,38(4):278-282,299
[25]丁成斌,熊光同,王强.毛叶蔷薇花净油化学成分的研究[J].香料香精化妆品,1991,1:1-4DING Cheng-bin,XIONG Guang-tong,WANG Qiang.Study on chemical compounds of the aromatic from petals of Rosa mairei Levl[J].Essential Spices Cosmetics,1991,1:1-4