功能性消化不良肝郁脾虚证模型的建立
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摘要
目的:探讨建立功能性消化不良(FD)肝郁脾虚证的动物模型方法。方法:健康SD大鼠30只随机分为5组:高脂组:高脂+束缚+游泳、番泻叶组:番泻叶煎剂+束缚+游泳、复合组:高脂+番泻叶煎剂+束缚+游泳、夹尾对照组:夹尾刺激+束缚+游泳、空白对照组。观察大鼠的饮食、大便、毛发、精神状态,检测胃排空率、小肠推进率及组织病理观察。结果:该造模方法制造的模型,符合FD动物模型的生物学特征。与空白对照组比较,复合组大鼠进食量显著减少(P<0.05),同时胃排空和小肠推进显著减缓(P<0.01)。病理显示未见器质性损伤。结论:成功建立了复合多因素FD肝郁脾虚证的动物模型,并且在周期上、安全性、复制难度、重复性等方面优于传统造模方法。
Objective: To establish an animal model for functional dyspepsia(FD) with liver depression and spleen deficiency. Methods: A total of 30 healthy SD rats were randomly divided into 5 groups: high fat group: high fat+tethered+swimming group, Fanxieye group: Fanxieye Fried Agent+tethered+swimming group, compound group: high fat+Fanxiye Fried Agent+tethered+swimming, clamp control group: clip tail stimulation+restraint+swimming, blank control group. The dietary, stool, hair and mental state of the rats were observed. The gastric motility, the small intestine propulsion rate and the tissue pathology excitability were measured and compared with the blank group. Results: The model of the modeling method was consistent with the biological characteristics of the FD animal model. Compared with the blank control group, the feeding of compound group was significantly decreased(P<0.05), and gastric emptying and small intestine of compound group were slowed down(P<0.01). Pathology showed no organic damage. Conclusion: The animal model of compound multivariate functional dyspepsia with liver depression and spleen deficiency is successfully established, and it is superior to the traditional modeling method in terms of cycle, safety, difficulty of replication and repeatability.
Objective: To establish an animal model for functional dyspepsia(FD) with liver depression and spleen deficiency. Methods: A total of 30 healthy SD rats were randomly divided into 5 groups: high fat group: high fat+tethered+swimming group, Fanxieye group: Fanxieye Fried Agent+tethered+swimming group, compound group: high fat+Fanxiye Fried Agent+tethered+swimming, clamp control group: clip tail stimulation+restraint+swimming, blank control group. The dietary, stool, hair and mental state of the rats were observed. The gastric motility, the small intestine propulsion rate and the tissue pathology excitability were measured and compared with the blank group. Results: The model of the modeling method was consistent with the biological characteristics of the FD animal model. Compared with the blank control group, the feeding of compound group was significantly decreased(P<0.05), and gastric emptying and small intestine of compound group were slowed down(P<0.01). Pathology showed no organic damage. Conclusion: The animal model of compound multivariate functional dyspepsia with liver depression and spleen deficiency is successfully established, and it is superior to the traditional modeling method in terms of cycle, safety, difficulty of replication and repeatability.
引文
[1]王小娟,周恒,郭璇,等.舒胃汤对功能性消化不良大鼠胃肠动力及ENS-ICC-SMC网络超微结构的影响.中国实验方剂学杂志,2014,20(24):134-139
[2]郭璇,刘芸,王小娟,等.舒胃汤对FD大鼠小肠深部肌间神经丛胆碱能神经-Cajal间质细胞-平滑肌网络结构损伤的影响.中国中西医结合杂志,2016,36(4):454-459
[3]郭璇,王小娟,胡淑娟,等.舒胃汤对功能性消化不良大鼠胃排空、胃动素和P物质表达的影响.世界中西医结合杂志,2011,6(8):663-665
[4]吴佳欣.慢性应激肝郁脾虚模型大鼠下丘脑弓状核摄食相关神经肽变化机制研究.石家庄:河北医科大学,2015
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[12]宋云娟.肝郁脾虚证的病理机制及临床治疗探讨.云南中医中药杂志,2012,33(10):16-17
[13]麻仲学,杨国红.胃脘痛的流行病学研究--附1544例调查普查分析报告(续).辽宁中医杂志,1985(12):26-28
[14]Hui Zhou,Shiyi Zhou.Upregulation of bile acid receptor TGR5and nNOS in gastric myenteric plexus is responsible for delayed gastric emptying after chronic high-fat feeding in rats.Am J Physiol Gastrointest Liver Physiol,2015,308:G863-G873
[15]李培彩,卢小芳,张声生,等.腹泻型肠易激综合征大鼠模型的评价研究.中国中西医结合消化杂志,2016,24(3):174-177
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[17]全俊.D-IBS与FD重叠肝郁脾虚证动物模型的建立及其中药干预机制研究.扬州:扬州大学,2015
[18]唐洪梅,廖小红,房财富,等.肝郁脾虚型大鼠肠易激综合征模型的建立及评价.中国实验方剂学杂志,2012,18(16):138-140
[19]刘干中,王建华,周金黄.世纪之交:现代中药药理学的回顾与展望.基础医学与临床,2000,20(5):1-3
[20]罗淑梅,耿怀霞,杨小霞.番泻叶的泻下作用机理及新应用.中华实用医药杂志,2004,4(8):728
[21]Wang L,Yao Q,Ye D,et al.Effects of Rifaximin on visceral sensitivity of rats with diarrhea induced by folium sennae.International Journal of Clinical&Experimental Medicine,2016,9(7):13986-13992
[2]郭璇,刘芸,王小娟,等.舒胃汤对FD大鼠小肠深部肌间神经丛胆碱能神经-Cajal间质细胞-平滑肌网络结构损伤的影响.中国中西医结合杂志,2016,36(4):454-459
[3]郭璇,王小娟,胡淑娟,等.舒胃汤对功能性消化不良大鼠胃排空、胃动素和P物质表达的影响.世界中西医结合杂志,2011,6(8):663-665
[4]吴佳欣.慢性应激肝郁脾虚模型大鼠下丘脑弓状核摄食相关神经肽变化机制研究.石家庄:河北医科大学,2015
[5]郭海军,林洁,李国成,等.功能性消化不良的动物模型研究.中国中西医结合消化杂志,2001,9(3):141-142
[6]郭宇,杨俭勤,李依洁,等.功能性消化不良动物模型在我国的应用概况.世界中西医结合杂志,2015,10(8):1182-1184
[7]李宇航,王庆国,陈萌,等.大鼠胃电节律失常模型胃肌间Cajal间质细胞含量的变化.世界华人消化杂志,2004,12(3):141-143
[8]张勇,王振华.大鼠胃电节律失常模型的建立.华人消化杂志,1998,6(7):64-65
[9]王煜姣,凌江红,张钰琴,等.复合病因造模法制备功能性消化不良大鼠模型.世界华人消化杂志,2014,22(2):210-214
[10]李艳彦,谢鸣,陈禹,等.一种运用复合病因造模法复制大鼠肝郁脾虚证模型的研究.中国中医基础医学杂志,2006,14(6):439-442
[11]李享,彭美哲,王思玉,等.肝郁脾虚证研究现状.中国中医药信息杂志,2013,20(11):107-109
[12]宋云娟.肝郁脾虚证的病理机制及临床治疗探讨.云南中医中药杂志,2012,33(10):16-17
[13]麻仲学,杨国红.胃脘痛的流行病学研究--附1544例调查普查分析报告(续).辽宁中医杂志,1985(12):26-28
[14]Hui Zhou,Shiyi Zhou.Upregulation of bile acid receptor TGR5and nNOS in gastric myenteric plexus is responsible for delayed gastric emptying after chronic high-fat feeding in rats.Am J Physiol Gastrointest Liver Physiol,2015,308:G863-G873
[15]李培彩,卢小芳,张声生,等.腹泻型肠易激综合征大鼠模型的评价研究.中国中西医结合消化杂志,2016,24(3):174-177
[16]李艳彦.肝郁脾虚证的模型复制及相关方剂作用的生物学基础.北京:北京中医药大学,2006
[17]全俊.D-IBS与FD重叠肝郁脾虚证动物模型的建立及其中药干预机制研究.扬州:扬州大学,2015
[18]唐洪梅,廖小红,房财富,等.肝郁脾虚型大鼠肠易激综合征模型的建立及评价.中国实验方剂学杂志,2012,18(16):138-140
[19]刘干中,王建华,周金黄.世纪之交:现代中药药理学的回顾与展望.基础医学与临床,2000,20(5):1-3
[20]罗淑梅,耿怀霞,杨小霞.番泻叶的泻下作用机理及新应用.中华实用医药杂志,2004,4(8):728
[21]Wang L,Yao Q,Ye D,et al.Effects of Rifaximin on visceral sensitivity of rats with diarrhea induced by folium sennae.International Journal of Clinical&Experimental Medicine,2016,9(7):13986-13992