湖南省部分发病猪猪链球菌血清型的调查
摘要
为了解猪链球菌病在湖南病猪群中不同血清型的流行情况,实验室于2015—2017年从湖南省部分病猪群中收集到疑似链球菌感染病料142份,分别进行细菌分离,并采用猪链球菌GDH特异性引物鉴定到106株猪链球菌,阳性率为74.65%(106/142)。然后采用猪链球菌33个血清型的特异性引物对这106个菌株进行PCR鉴定,结果 86.79%的菌株被鉴定到具体血清型,其中优势血清型为2型(1/2型)30.19%,其次是7型(11.32%)、8型(9.43%)和3型(6.60%)。
引文
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[2]Hill J E,Gottschalk M,Brousseau R,et al.Biochemical analysis,cpn60 and 16S rDNA sequence data indicate that Streptococcus suis serotypes 32 and 34,isolated from pigs,are Streptococcus orisratti[J].Veterinary Microbiology,2005,107(1-2):63-69.
[3]陆承平.兽医微生物学[M].第4版.北京:中国农业出版社,2007:101.
[4]张超颖,徐玉花,蒋春燕.2型猪链球菌病的研究进展[J].畜禽业,2015(3):20-22.
[5]Wei Zigong,Li Ran,Zhang Anding,et al.Char acterization of Streptococcus suis isolates from the diseased pigs in China between 2003 and 2007[J].Veterinary Microbiology,2009,137(1-2):196-201.
[6]Berthelot-Hérault F,Morvan H,Kéribin A M,et al.Production of Muraminidase-Released Protein(MRP),Extracellular Factor(EF)and Suilysin by field isolates of Streptococcus suis capsular types 2,1/2,9,7 and 3 isolated from swine in France[J].Veterinary Research,2000,31(5):473-479.
[7]Aarestrup,F M,Jorsal,S E,Jensen,N E.Serological characterization and antimicrobial susceptibility of Streptococcus suis isolates from diagnostic samples in Denmark during 1995 and1996[J].Veterinary Microbiology,1998,60(1):59-66.
[8]Kataoka Y,Sugimoto C,Nakazawa M,et al.The epidemiological studies of Streptococcus suis infections in Japan from 1987 to1991[J].Journal of Veterinary Medical Science,1993,55(4):623.
[9]Kim D,Han K,Oh Y,et al.Distribution of capsular serotypes and virulence markers of Streptococcus suis isolated from pigs with polyserositis in Korea[J].Can J Vet Res,2010,74(4):314-316.
[10]王花茹,王长军,唐家琪,等.猪链球菌种及其主要致病血清型多重PCR检测方法的建立[J].畜牧兽医学报,2008(6):837-841.
[11]Liu Zhijie,Zhang Han,Gottschalk Marcelo,et al.Development of Multiplex PCR Assays for the Identification of the 33Serotypes of Streptococcus suis[J].Plos One,2013(8):e72070.
[12]王娟,刘琪,周如月,等.广东地区猪群中猪链球菌流行病学调查分析[J].中国预防兽医学报,2017(8):621-623.
[13]董志民,杨春蕾,田向学,等.猪链球菌的分离鉴定及其致病性分子基础研究[J].中国预防兽医学报,2018(2):96-100.
[14]徐引弟,孙帅杰,王治方,等.河南省猪链球菌的分离鉴定及耐药性分析[J].中国畜牧兽医,2018(2):501-509.