27份紫花苜蓿种质资源萌发期抗旱性评价
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摘要
以27份紫花苜蓿(Medicago sativa L.)种质资源为试材,利用10%的聚乙二醇(PEG-6000)溶液模拟干旱胁迫条件开展了紫花苜蓿萌发期抗旱性研究;以相对发芽率、萌发胁迫指数、萌发抗旱指数和活力抗旱指数作为苜蓿抗旱性鉴定的指标,并运用隶属函数法对紫花苜蓿抗旱性进行了综合评价。结果表明,27份紫花苜蓿试材萌发期抗旱性存在显著差异,属于高抗旱性的种质材料有4份,分别是西宁、肇东、中苜1号和石家庄;属于中等抗旱水平的11份;属于抗旱性较弱的12份。地方品种资源中蕴藏着优异抗旱基因,充分挖掘地方品种资源是筛选和培育抗旱紫花苜蓿品种的有效途径。
The experiment was conducted to estimate drought resistance of 27 alfalfa germplasm resources at seed germination stage by simulating drought stress with 10% polyethylene glycol( PEG-6000) solution, and relative germination rate, germination stress index, germination drought resistance index and vigor drought resistance index were measured, and the comprehensive valuation for drought resistance of each germplasm resource was conducted by weighting membership function analysis. The results show that there are obvious differences for drought resistance at seed germination stage among 27 alfalfa germplasm resources, and four with highly resistance, including Xining, Zaodong, Zhongmu No.1 and Shijiazhuang; eleven with middle resistance; twelve with weak resistance. Drought-tolerant gene resources may be found in the country varietal resources, and this is a effective approach of cultivating resistant varieties of alfalfa.
The experiment was conducted to estimate drought resistance of 27 alfalfa germplasm resources at seed germination stage by simulating drought stress with 10% polyethylene glycol( PEG-6000) solution, and relative germination rate, germination stress index, germination drought resistance index and vigor drought resistance index were measured, and the comprehensive valuation for drought resistance of each germplasm resource was conducted by weighting membership function analysis. The results show that there are obvious differences for drought resistance at seed germination stage among 27 alfalfa germplasm resources, and four with highly resistance, including Xining, Zaodong, Zhongmu No.1 and Shijiazhuang; eleven with middle resistance; twelve with weak resistance. Drought-tolerant gene resources may be found in the country varietal resources, and this is a effective approach of cultivating resistant varieties of alfalfa.
引文
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[17] 代小冬,杨育峰,朱灿灿,等.谷子萌芽期对干旱胁迫的响应及抗旱性评价[J].华北农学报,2015,30(4):139～144.
[18] 李龙,王兰芬,武晶,等.普通菜豆种质资源芽期抗旱性鉴定[J].植物遗传资源学报,2013,14(4):600～605.
[19] 王兰芬,武晶,景蕊莲,等.绿豆种质资源芽期抗旱性鉴定[J].植物遗传资源学报,2014,15(3):498～503.
[20] 成广雷,张海娇,赵久然,等.临界胁迫贮藏条件下不同基因型玉米种子活力及生理变化[J].中国农业科学2015,48(1):33～42.
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[23] 李造哲.1 0种苜蓿品种幼苗抗旱性的研究[J].中国草地,1991(3):1～3.
[24] 孟林,毛培春,张国芳,等.17 个苜蓿品种苗期抗旱性鉴定[J].草业科学,2008,25(1):21～25.
[25] 张恩厚.我国北部16个紫花苜蓿品种材料的数量分类研究[J].中国草地学报,1988(6):4～9.
[26] 徐向南,易津,于林清,等.不同种质苜蓿苗期耐旱性评价及其方法的比较[J].草业科学,2011,28(2):217～225.
[27] 韩德梁,王彦荣.紫花苜蓿对干旱胁迫适应性的研究进展[J].草业学报,2005,14(6):7～13.
[2] 郭彦军,倪郁,吕俊,等.豆科牧草种子萌发特性与其抗旱性差异的研究[J].中国草地,2003,25(3):24～27.
[3] 余贵海,起雪宏,王正启,等.14 个玉米杂交种萌发期抗旱性评价[J].西南农业学报,2016,29(7):1499～1505.
[4] 石运庆,王建,苗华荣,等.不同花生品种芽期抗旱性筛选及其田间验证[J].山东农业科学,2015,47(2):34～37.
[5] 李俐俐,刘天学,赵霞.大豆种子萌发期对渗透胁迫的响应[J].大豆科学,2007,26(4):550～554.
[6] 李培英,孙宗玖,阿不来提.PEG模拟干旱胁迫下29份偃麦草种质种子萌发期抗旱性评价[J].中国草地学报,2010,32(1):32～39.
[7] 王文帆,梁素钰,刘滨凡,等.聚乙二醇对谷稗种子萌发及相关生理指标的影响[J].中国农学通报,2010,26(12):122～125.
[8] 牛瑞明,王燕,吴桂丽,等.裸燕麦种子萌发对模拟干旱胁迫的响应及其耐旱性综合评价[J].麦类作物学报,2011,31(4):753～756.
[9] 蔡丽艳,李志勇,孙启忠,等.扁蓿豆萌发对干旱胁迫的响应及抗旱性评价[J].草业科学,2012,29(10):1553～1559.
[10] 李文娆,张岁岐,山仑.水分胁迫下紫花苜蓿和高粱种子萌发特性及幼苗耐旱性.生态学报,2009,29(6):3066～3073.
[11] 韩瑞宏,卢欣石,高桂娟,等.紫花苜蓿抗旱性主成分及隶属函数分析[J].草地学报,14(2):142～146.
[12] 刘卓,王志锋,耿慧,等.15个紫花苜蓿品种抗旱性评价[J].安徽农业科学,2009,37(35):17442～17444.
[13] 孟林,毛培春,张国芳,等.17 个苜蓿品种苗期抗旱性鉴定[J].草业科学,2008,25(1):21～25.
[14] 张鹤山,陈明新,王凤,等.18 个紫花苜蓿品种苗期抗旱性综合评价[J].江苏农业科学,40(3):168～170.
[15] 孙艳茹,石屹,陈国军,等.PEG模拟干旱胁迫下8种绿肥作物萌发特性与抗旱性评价[J].草业学报,2015,24(3):89～98.
[16] 张健,池宝亮,黄学芳,等.玉米萌芽期水分胁迫的抗旱性分析[J].山西农业科学2007,35(2):34～38.
[17] 代小冬,杨育峰,朱灿灿,等.谷子萌芽期对干旱胁迫的响应及抗旱性评价[J].华北农学报,2015,30(4):139～144.
[18] 李龙,王兰芬,武晶,等.普通菜豆种质资源芽期抗旱性鉴定[J].植物遗传资源学报,2013,14(4):600～605.
[19] 王兰芬,武晶,景蕊莲,等.绿豆种质资源芽期抗旱性鉴定[J].植物遗传资源学报,2014,15(3):498～503.
[20] 成广雷,张海娇,赵久然,等.临界胁迫贮藏条件下不同基因型玉米种子活力及生理变化[J].中国农业科学2015,48(1):33～42.
[21] Bouslarna M.Stress tolerance in soybeans[J].Evaluation crop Sci,1984,24:933～937.
[22] 李国瑞,马宏亮,胡雯媚,等.西南麦区小麦品种萌发期抗旱性综合鉴定[J].麦类作物报,2015,35(4):1～9.
[23] 李造哲.1 0种苜蓿品种幼苗抗旱性的研究[J].中国草地,1991(3):1～3.
[24] 孟林,毛培春,张国芳,等.17 个苜蓿品种苗期抗旱性鉴定[J].草业科学,2008,25(1):21～25.
[25] 张恩厚.我国北部16个紫花苜蓿品种材料的数量分类研究[J].中国草地学报,1988(6):4～9.
[26] 徐向南,易津,于林清,等.不同种质苜蓿苗期耐旱性评价及其方法的比较[J].草业科学,2011,28(2):217～225.
[27] 韩德梁,王彦荣.紫花苜蓿对干旱胁迫适应性的研究进展[J].草业学报,2005,14(6):7～13.