玉米低温响应NAC转录因子的分子特性分析
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摘要
利用Illumina HiSeq技术,分别构建5℃和22℃处理下的两个玉米叶组织转录组。从转录组数据中共分离出38条低温响应NAC基因,包括6条膜结合转录因子;38条NAC中30条为上调,8条为下调。38条ZmNACs被分为12个亚族(NAC a-1),其中,7条上调低温响应ZmNACs属于逆境相关亚家族(Stress related/NAC-h)。同一亚家族内,蛋白结构域和内含子/外显子结构相对保守。不同ZmNAC基因的在不同组织部位和发育阶段的表达模式有很大变化。许多重要的非生物胁迫相关元件在38条ZmNACs的启动子中高度富集。
NAM, ATAF1,-2, and CUC2(NAC) proteins are plant-specific transcription factors that play important roles in different developmental processes and stress responses. In this study, Illumina Hi Seq technology was used to construct two transcriptomes of maize leaf tissue treated at 5 and 22℃, respectively. From the transcriptome data, 38 low-temperature response NAC genes were isolated, including 6 membrane-bound transcription factors.Among the 38 NACs, 30 were up-regulated and 8 were down-regulated. Thirty-eight Zm NACs were divided into 12 subfamilies(NAC a-1), of which 7 up-regulated low-temperature response Zm NACs belonged to stress related/NAC-h. Within the same subfamily, protein domains and intron/exon structures are relatively conserved. The expression patterns of different Zm NAC genes at different tissue sites and at developmental stages vary greatly. Many important elements in abiotic stress are highly enriched in the promoters of 38 Zm NACs. This study will help deepen the understanding of the maize NAC gene family and provide clues for further functional studies.
NAM, ATAF1,-2, and CUC2(NAC) proteins are plant-specific transcription factors that play important roles in different developmental processes and stress responses. In this study, Illumina Hi Seq technology was used to construct two transcriptomes of maize leaf tissue treated at 5 and 22℃, respectively. From the transcriptome data, 38 low-temperature response NAC genes were isolated, including 6 membrane-bound transcription factors.Among the 38 NACs, 30 were up-regulated and 8 were down-regulated. Thirty-eight Zm NACs were divided into 12 subfamilies(NAC a-1), of which 7 up-regulated low-temperature response Zm NACs belonged to stress related/NAC-h. Within the same subfamily, protein domains and intron/exon structures are relatively conserved. The expression patterns of different Zm NAC genes at different tissue sites and at developmental stages vary greatly. Many important elements in abiotic stress are highly enriched in the promoters of 38 Zm NACs. This study will help deepen the understanding of the maize NAC gene family and provide clues for further functional studies.
引文
[1]Ooka H.Comprehensive analysis of NAC family genes in Oryza sativa and Arabidopsis thaliana[J].DNA Res,2003,10(6):239-247.
[2]Seo P J,Park C M.A membrane-bound NAC transcription factor as an integrator of biotic and abiotic stress signals[J].Plant Signal Behav,2010,5(5):481-483.
[3]Lei C,Yang Y,Xiaodong D,et al.The Glycine soja NAC transcription factor Gs NAC019 mediates the regulation of plant alkaline tolerance and ABA sensitivity[J].Plant Molecular Biology,2017,95(3):253-268.
[4]Venkatesh P T,Vikas D,Nikolay M,et al.NAC transcription factor JUNGBRUNNEN1 enhances drought tolerance in tomato[J].Plant Biotechnology Journal,2017,16(2):354-366.
[5]Saghar E M,Pamela A R,Venkatesh P T,et al.JUNGBRUNNEN1Confers drought tolerance downstream of the HD-Zip I transcription factor At HB13[J].Front Plant Sci.,2017,8:2118.
[6]Tran L S,Nakashima K,Sakuma Y,et al.Isolation and functional analysis of Arabidopsis stress-inducible NAC transcription factors that bind to a drought responsive cis-element in the early responsive to dehydration stress promoter[J].Plant Cell,2004,16(9):2481-2498.
[7]Takasaki H,Maruyama K,Kidokoro S,et al.The abiotic stress-responsive NAC-type transcription factor Os NAC5 regulates stress-inducible genes and stress tolerance in rice[J].Mol Genet Gen,2010,284(3):173-183.
[8]Song S Y,Chen Y,Chen J,et al.Physiological mechanisms underlying Os NAC5-dependent tolerance of rice plants to abiotic stress[J].Planta,2011,234(2):331-345.
[9]Huang L,Hong Y,Zhang H,et al.Rice NAC transcription factor ONAC095 plays opposite roles in drought and cold stress tolerance[J].BMC Plant Biol,2016,16:203.
[10]Lee D K,Chung P J,Jeong J S,et al.The rice Os NAC6 transcription factor orchestrates multiple molecular mechanisms involving root structural adaptions and nicotianamine biosynthesis for drought tolerance[J].Plant Biotechnol J,2017,15(6):754-764.
[11]Liu G,Li X,Jin S,et al.Overexpression of rice NAC gene SNAC1improves drought and salt tolerance by enhancing root development and reducing transpiration rate in transgenic cotton[J].PLo S One,2014,9(1):86-89.
[12]Nakashima K,Tran L S,Van Nguyen D,et al.Functional analysis of a NAC-type transcription factor Os NAC6 involved in abiotic and biotic stress-responsive gene expression in rice[J].Plant J,2007,51(4):617-630.
[13]Jeong J S,Kim Y S,Redillas M C,et al.Os NAC5 overexpression enlarges root diameter in rice plants leading to enhanced drought tolerance and increased grain yield in the field[J].Plant Biotechnol J,2013,11(1):101-14.
[14]Wu Y,Deng Z,Lai J,et al.Dual function of Arabidopsis ATAF1 in abiotic and biotic stress responses[J].Cell Res,2009,19(11):1279-90.
[15]Delessert C,Kazan K,Wilson I W,et al.The transcription factor ATAF2 represses the expression of pathogenesis-related genes in Arabidopsis[J].Plant J,2005,43(5):745-57.
[16]Mao H D,Yu L J,Han R,et al.Zm NAC55,a maize stress-responsive NAC transcription factor,confers drought resistance in transgenic Arabidopsis[J].Plant Physiology and Biochemistry,2016,105:55-66.
[17]Fang Y,You J,Xie K,et al.Systematic sequence analysis and identification of tissue-specific or stress-responsive genes of NAC transcription factor family in rice[J].Mol.Genet.Genomics,2008,280(6):535-546.
[18]Zhou Q Y,Tian A G,Zou H F,et al.Soybean WRKY-type transcription factor genes,Gm WRKY13,Gm WRKY21,and Gm WRKY54,confer differential tolerance to abiotic stresses in transgenic Arabidopsis plants[J].Plant Biotechnol.J,2008,6(5):486-503.
[19]Fujita M,Fujita Y,Maruyama K,et al.A dehydration-induced NAC protein,RD26,is involved in a novel ABA-dependent stresssignaling pathway[J].Plant J,2004,39(6):863-876.
[20]Tran L S,Nakashima K,Sakuma Y,et al.Isolation and functional analysis of Arabidopsis stress-inducible NAC transcription factors that bind to a drought responsive cis-element in the early responsive to dehydration stress promoter[J].Plant Cell,2004,16(9):2481-2498.
[21]Bu Q,Jiang H,Li C B,et al.Role of the Arabidopsis thaliana NACtranscription factors ANAC019 and ANAC055 in regulating jasmonic acid-signaled defense responses[J].Cell Res,2008,18:756-767.
[22]Mao H D,Wang H W,Liu S X,et al.A transposable element in a NAC gene is associated with drought tolerance in maize seedlings[J].Nture Communcation,2015,6:9326.
[23]Lu M,Ying S,Zhang D F,et al.A maize stress-responsive NACtranscription factor,Zm SNAC1,confers enhanced tolerance to dehydration in transgenic Arabidopsis[J].Plant Cell Rep,2012,31(9):1701-1711.
[24]Puranik S,Sahu P P,Srivastava P S,et al.NAC proteins:regulation and role in stress tolerance[J].Trends Plant Sci.,2012,17(6):369-381.
[25]Chen Y N,Slabaugh E,Brandizzi F.Membrane-tethered transcription factors in Arabidopsis thaliana:novel regulators in stress response and development,Curr.Opin[J].Plant Biol,2008,11(6):695-701.
[26]Kim M J,Park M J,Seo P J,et al.Controlled nuclear import of the transcription factor NTL6 reveals a cytoplasmic role of Sn RK2.8 in the drought-stress response[J].Biochem J,2012,448(3):353-363.
[27]Park J,Kim Y S,Kim S G,et al.Integration of auxin and salt signals by the NAC transcription factor NTM2 during seed germination in Arabidopsis[J].Plant Physiol,2011,176(1):537-549.
[28]Yang Z T,Lu S J,Wang M J,et al.A plasma membrane-tethered transcription factor,NAC062/ANAC062/NTL6,mediates the unfolded protein response in Arabidopsis[J].Plant J,2014,76(6):1033-1043.
[29]He L,Su C,Wang Y C,et al.ATDOF5.8 protein is the upstream regulator of ANAC069 and is responsive to abiotic stress[J].Biochimie,2015,110:17-24.
[30]Liao Y,Zou H F,Wei W,et al.Soybean Gmb ZIP44,Gmb ZIP62and Gmb ZIP78 genes function as negative regulator of ABA signaling and confer salt and freezing tolerance in transgenic Arabidopsis[J].Planta,2008,228(2):225-240.
[2]Seo P J,Park C M.A membrane-bound NAC transcription factor as an integrator of biotic and abiotic stress signals[J].Plant Signal Behav,2010,5(5):481-483.
[3]Lei C,Yang Y,Xiaodong D,et al.The Glycine soja NAC transcription factor Gs NAC019 mediates the regulation of plant alkaline tolerance and ABA sensitivity[J].Plant Molecular Biology,2017,95(3):253-268.
[4]Venkatesh P T,Vikas D,Nikolay M,et al.NAC transcription factor JUNGBRUNNEN1 enhances drought tolerance in tomato[J].Plant Biotechnology Journal,2017,16(2):354-366.
[5]Saghar E M,Pamela A R,Venkatesh P T,et al.JUNGBRUNNEN1Confers drought tolerance downstream of the HD-Zip I transcription factor At HB13[J].Front Plant Sci.,2017,8:2118.
[6]Tran L S,Nakashima K,Sakuma Y,et al.Isolation and functional analysis of Arabidopsis stress-inducible NAC transcription factors that bind to a drought responsive cis-element in the early responsive to dehydration stress promoter[J].Plant Cell,2004,16(9):2481-2498.
[7]Takasaki H,Maruyama K,Kidokoro S,et al.The abiotic stress-responsive NAC-type transcription factor Os NAC5 regulates stress-inducible genes and stress tolerance in rice[J].Mol Genet Gen,2010,284(3):173-183.
[8]Song S Y,Chen Y,Chen J,et al.Physiological mechanisms underlying Os NAC5-dependent tolerance of rice plants to abiotic stress[J].Planta,2011,234(2):331-345.
[9]Huang L,Hong Y,Zhang H,et al.Rice NAC transcription factor ONAC095 plays opposite roles in drought and cold stress tolerance[J].BMC Plant Biol,2016,16:203.
[10]Lee D K,Chung P J,Jeong J S,et al.The rice Os NAC6 transcription factor orchestrates multiple molecular mechanisms involving root structural adaptions and nicotianamine biosynthesis for drought tolerance[J].Plant Biotechnol J,2017,15(6):754-764.
[11]Liu G,Li X,Jin S,et al.Overexpression of rice NAC gene SNAC1improves drought and salt tolerance by enhancing root development and reducing transpiration rate in transgenic cotton[J].PLo S One,2014,9(1):86-89.
[12]Nakashima K,Tran L S,Van Nguyen D,et al.Functional analysis of a NAC-type transcription factor Os NAC6 involved in abiotic and biotic stress-responsive gene expression in rice[J].Plant J,2007,51(4):617-630.
[13]Jeong J S,Kim Y S,Redillas M C,et al.Os NAC5 overexpression enlarges root diameter in rice plants leading to enhanced drought tolerance and increased grain yield in the field[J].Plant Biotechnol J,2013,11(1):101-14.
[14]Wu Y,Deng Z,Lai J,et al.Dual function of Arabidopsis ATAF1 in abiotic and biotic stress responses[J].Cell Res,2009,19(11):1279-90.
[15]Delessert C,Kazan K,Wilson I W,et al.The transcription factor ATAF2 represses the expression of pathogenesis-related genes in Arabidopsis[J].Plant J,2005,43(5):745-57.
[16]Mao H D,Yu L J,Han R,et al.Zm NAC55,a maize stress-responsive NAC transcription factor,confers drought resistance in transgenic Arabidopsis[J].Plant Physiology and Biochemistry,2016,105:55-66.
[17]Fang Y,You J,Xie K,et al.Systematic sequence analysis and identification of tissue-specific or stress-responsive genes of NAC transcription factor family in rice[J].Mol.Genet.Genomics,2008,280(6):535-546.
[18]Zhou Q Y,Tian A G,Zou H F,et al.Soybean WRKY-type transcription factor genes,Gm WRKY13,Gm WRKY21,and Gm WRKY54,confer differential tolerance to abiotic stresses in transgenic Arabidopsis plants[J].Plant Biotechnol.J,2008,6(5):486-503.
[19]Fujita M,Fujita Y,Maruyama K,et al.A dehydration-induced NAC protein,RD26,is involved in a novel ABA-dependent stresssignaling pathway[J].Plant J,2004,39(6):863-876.
[20]Tran L S,Nakashima K,Sakuma Y,et al.Isolation and functional analysis of Arabidopsis stress-inducible NAC transcription factors that bind to a drought responsive cis-element in the early responsive to dehydration stress promoter[J].Plant Cell,2004,16(9):2481-2498.
[21]Bu Q,Jiang H,Li C B,et al.Role of the Arabidopsis thaliana NACtranscription factors ANAC019 and ANAC055 in regulating jasmonic acid-signaled defense responses[J].Cell Res,2008,18:756-767.
[22]Mao H D,Wang H W,Liu S X,et al.A transposable element in a NAC gene is associated with drought tolerance in maize seedlings[J].Nture Communcation,2015,6:9326.
[23]Lu M,Ying S,Zhang D F,et al.A maize stress-responsive NACtranscription factor,Zm SNAC1,confers enhanced tolerance to dehydration in transgenic Arabidopsis[J].Plant Cell Rep,2012,31(9):1701-1711.
[24]Puranik S,Sahu P P,Srivastava P S,et al.NAC proteins:regulation and role in stress tolerance[J].Trends Plant Sci.,2012,17(6):369-381.
[25]Chen Y N,Slabaugh E,Brandizzi F.Membrane-tethered transcription factors in Arabidopsis thaliana:novel regulators in stress response and development,Curr.Opin[J].Plant Biol,2008,11(6):695-701.
[26]Kim M J,Park M J,Seo P J,et al.Controlled nuclear import of the transcription factor NTL6 reveals a cytoplasmic role of Sn RK2.8 in the drought-stress response[J].Biochem J,2012,448(3):353-363.
[27]Park J,Kim Y S,Kim S G,et al.Integration of auxin and salt signals by the NAC transcription factor NTM2 during seed germination in Arabidopsis[J].Plant Physiol,2011,176(1):537-549.
[28]Yang Z T,Lu S J,Wang M J,et al.A plasma membrane-tethered transcription factor,NAC062/ANAC062/NTL6,mediates the unfolded protein response in Arabidopsis[J].Plant J,2014,76(6):1033-1043.
[29]He L,Su C,Wang Y C,et al.ATDOF5.8 protein is the upstream regulator of ANAC069 and is responsive to abiotic stress[J].Biochimie,2015,110:17-24.
[30]Liao Y,Zou H F,Wei W,et al.Soybean Gmb ZIP44,Gmb ZIP62and Gmb ZIP78 genes function as negative regulator of ABA signaling and confer salt and freezing tolerance in transgenic Arabidopsis[J].Planta,2008,228(2):225-240.