不同光照强度对甜荞内源激素、产量和品质的影响
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摘要
为了明确光照强度对甜荞内源激素、产量和品质的影响,以高产甜荞品种‘丰甜1号’为试验材料,设置了遮阴、常规和增光3个光照处理,测定‘丰甜1号’成熟期根茎叶中的脱落酸、玉米素+玉米素核苷、生长素、1-氨基环丙烷-1-羧酸、多胺等内源激素含量和最终的产量形成指标和品质,最后对内源激素与甜荞产量和品质的关系进行了相关性分析。结果表明:‘丰甜1号’的单株粒重、千粒重、产量、总膳食纤维、蛋白质、总淀粉和黄酮含量均随光照强度的增加而增加;增加光照处理能明显增加‘丰甜1号’各部位中的ACC含量,但降低了ABA含量,光照处理对‘丰甜1号’各部位的IAA、Z+ZR和多胺的影响不大;‘丰甜1号’不同部位间的内源激素含量存在明显差异。相关性研究结果表明,根茎叶中的ABA含量与‘丰甜1号’的产量和品质呈负相关关系,IAA、ACC等内源激素含量与‘丰甜1号’的产量和品质呈正相关关系,部分达极显著正相关关系。
In order to identify the effects of light intensity on the endogenous hormones, yield and quality of common buckwheat, high-yield common buckwheat variety 'Fengtian 1' was used as material and was treated with 3 light intensities which were shading, normal light and enhanced light. Content of endogenous hormones such as ABA, Z+ZR, IAA, ACC and polyamine in leaves, stem and root at mature stage of 'Fengtian 1' were determined and the indicators and quality of final yield formation were also determined. Finally, correlation analysis on the relationship between endogenous hormones and yield and quality of common buckwheat were carried out in this study. The results showed that the grain weight per plant, 1 000 kernel weight, yield, total dietary fiber, protein, total starch and flavonoids contents of 'Fengtian 1' were significantly improved with the increasing of light. The content of ACC in each part of 'Fengtian 1' could be significantly improved with the increasing of light, while the content of ABA was reduced. Light treatment had little effect on IAA, Z+ZR and polyamines in each part of 'Fengtian1'. The content of endogenous hormones had a significantly different in different parts of 'Fengtian 1'. Correlation study results showed that there was a negative correlation between ABA content in root, stem and leaves and the yield and quality of 'Fengtian 1', and there was a positive correlation between IAA, ACC and other endogenous contents and the yield and quality of 'Fengtian 1', of which some showed extremely significant positive correlation.
In order to identify the effects of light intensity on the endogenous hormones, yield and quality of common buckwheat, high-yield common buckwheat variety 'Fengtian 1' was used as material and was treated with 3 light intensities which were shading, normal light and enhanced light. Content of endogenous hormones such as ABA, Z+ZR, IAA, ACC and polyamine in leaves, stem and root at mature stage of 'Fengtian 1' were determined and the indicators and quality of final yield formation were also determined. Finally, correlation analysis on the relationship between endogenous hormones and yield and quality of common buckwheat were carried out in this study. The results showed that the grain weight per plant, 1 000 kernel weight, yield, total dietary fiber, protein, total starch and flavonoids contents of 'Fengtian 1' were significantly improved with the increasing of light. The content of ACC in each part of 'Fengtian 1' could be significantly improved with the increasing of light, while the content of ABA was reduced. Light treatment had little effect on IAA, Z+ZR and polyamines in each part of 'Fengtian1'. The content of endogenous hormones had a significantly different in different parts of 'Fengtian 1'. Correlation study results showed that there was a negative correlation between ABA content in root, stem and leaves and the yield and quality of 'Fengtian 1', and there was a positive correlation between IAA, ACC and other endogenous contents and the yield and quality of 'Fengtian 1', of which some showed extremely significant positive correlation.
引文
Cheng C.Y.,and Lur H.S.,1996,Ethylene may be involved in abortion of the maize caryopsis,Physiol.Plant,98(2):245-252
Cheng Y.G.,Wang R.,and Meng L.,2012,Effect of light intensity on the growth and vitamin C content of safflower sprouts,Beifang Yuanyi(Northern Horticulture),(22):154-156(成元刚,王锐,孟丽,2012,光照强度对红花芽菜生长和维生素C含量的影响,北方园艺,(22):154-156)
Cui H.Y.,Jin L.B.,Li B.,Dong S.T.,Liu P.,Zhao B.,and Zhang J.W.,2013,Effects of shading on dry matter accumulation and nutrient absorption of summer maize,Yingyong Shengtai Xuebao(Chinese Journal of Applied Ecology),24(11):3099-3105(崔海岩,靳立斌,李波,董树亭,刘鹏,赵斌,张吉旺,2013,遮阴对夏玉米干物质积累及养分吸收的影响,应用生态学报,24(11):3099-3105)
Dong M.H.,Zhao B.H.,Wu X.Z.,Chen T.,and Yang J.C.,2008,Difference in hormonal content and activities of key enzymes in the grains at different positions on a rice panicle during grain filling and their correlations with rice qualities,Zhongguo Nongye Kexue(Scientia Agricultura Sinica),41(2):370-380(董明辉,赵步洪,吴翔宙,陈涛,杨建昌,2008,水稻结实期不同粒位籽粒相关内源激素含量和关键酶活性的差异及其与品质的关系,中国农业科学,41(2):370-380)
Flores H.E.,and Galston A.W.,1982,Analysis of polyamines in higher plants by high performance liquid chromatography,Plant Physiol.,69(3):701-706
Gao J.,Shi J.G.,Dong S.T.,Liu P.,Zhao B.,and Zhang J.W.,2017,Effect of different light intensities on root characteristics and grain yield of summer maize(Zea mays L.),Zhongguo Nongye Kexue(Scientia Agricultura Sinica),50(11):2104-2113(高佳,史建国,董树亭,刘鹏,赵斌,张吉旺,2017,花粒期光照强度对夏玉米根系生长和产量的影响,中国农业科学,50(11):2104-2113)
He Z.P.,ed.,1993,Guidance to experiment on chemical control incrop plants,Beijing Agricultural University Press,Beijing,China,pp.60-68(何钟佩,编著,1993,农作物化学控制试验指导,北京农业大学出版社,中国,北京,pp.60-68)
Huang K.F.,Jiang J.Z.,Qin Y.L.,Yang K.,and Cao B.S.,2008,Effect of organic fertilizer and inorganic fertilizer on dietary fiber content of Zizania latifolia,Zhongguo Shucai(China Vegetables),1(4):17-20(黄凯丰,江解增,秦玉莲,杨凯,曹碚生,2008,有机肥和无机肥对茭白膳食纤维含量的影响,中国蔬菜,1(4):17-20)
Huang K.F.,Peng H.R.,Guo X.,Zhao G.,and Chen Q.F.,2013,Difference of hormonal content in two tartary buckwheat cultivars and their relations to yield and quality,Jiangsu Nongye Xuebao(Jiangsu Journal of Agricultural Sciences),29(1):28-32(黄凯丰,彭慧蓉,郭肖,赵钢,陈庆富,2013,2个苦荞品种成熟期内源激素的含量差异和品质的关系,江苏农业学报,29(1):28-32)
Huang X.Y.,Zeller F.J.,Huang K.F.,Shi T.X.,and Chen Q.F.,2014,Variation of major minerals and trace elements in seeds of tartary buckwheat(Fagopyrum tatarium Gaertn.),Genet.Resour.Crop Ev.,61(3):567-577
Liu Z.F.,Gou L.,Zhao M.,and Zhang B.J.,2011,Effects of shading on stalk morphological characteristics,rind penetra tion strength and lodging-resistance of maize,Huabei Nongxuebao(Acta Agriculturae Boreali-Sinica),26(4):91-96(刘仲发,勾玲,赵明,张保军,2011,遮阴对玉米茎秆形态特征,穿刺强度及抗倒伏能力的影响,华北农学报,26(4):91-96)
Luo X.P.,Zhu D.Y.,Huang Y.J.,Li M.F.,Yao P.F.,Gao F.,Li C.L.,and Zhao H.X.,2015,Effects of methyl jasmonate accumulation of flavonoids and related gene expression of buckwheat sprouts,Jiyinzuxue Yu Yingyong Shengwuxue(Ge nomics and Applied Biology),34(5):1040-1046(雒晓鹏,朱冬寅,黄云吉,李茂菲,姚攀锋,高飞,李成磊,赵海霞,2015,茉莉酸甲酯对芽期苦荞黄酮积累及相关基因表达的影响,基因组学与应用生物学,34(5):1040-1046)
Shi J.G.,Zhu K.L.,Cao H.Y.,Dong S.T.,Liu P.,Zhao B.,and Zhang J.W.,2015,Effect of light from flowering to maturity stage on dry matter accumulation and nutrient absorption of summer maize,Yingyong Shengtai Xuebao(Chinese Journal of Applied Ecology),26(1):46-52(史建国,朱昆仑,曹慧英,董树亭,刘鹏,赵斌,张吉旺,2015,花粒期光照对夏玉米干物质积累和养分吸收的影响,应用生态学报,26(1):46-52)
Wang Y.,Qi X.N.,Shao J.F.,Liu Z.Y.,and Li Z.X.,2008,Effects of light intensity at full growing stage on the growth and yield of different maize varieties,Jilin Nongye Daxue Xue bao(Journal of Jilin Agricultural University),30(6):769-773(王洋,齐晓宁,邵金锋,刘兆永,李泽兴,2008,光照强度对不同玉米品种生长发育和产量构成的影响,吉林农业大学学报,30(6):769-773)
Wang Z.Q.,Ye Y.X.,Yang J.C.,Yuan L.M.,Wang X.M.,and Zhu Q.S.,2004,Changes and regulation of sucrose synthase activity in grains during grain filling stage of rice,Zuowu Xuebao(Acta Agronomica Sinica),30(7):634-643(王志琴,叶玉秀,杨建昌,袁莉民,王学明,朱庆森,2004,水稻灌浆期籽粒中蔗糖合成酶活性的变化与调节,作物学报,30(7):634-643)
Yan J.,Wang D.L.,Hao J.X.,and Xie S.X.,2017,Effects of light intensity on fruit quality of rabbiteye blueberry in Guizhou,Jingjilin Yanjiu(Nonwood Forest Research),35(4):118-122(闫静,王德炉,郝家孝,谢双喜,2017,光照强度对贵州兔眼蓝莓果实品质的影响,经济林研究,35(4):118-122)
Yang J.C.,Chang E.H.,Tang C.,Zhang H.,and Wang Z.Q.,2007,Relationships of ethylene evolution rate and 1-aminocylo propane-l-carboxylic acid concentration in grains during grain filling with appearance quality of rice,Zhongguo Shuidao Kexue(Chinese Journal of Rice Science),21(1):77-83(杨建昌,常二华,唐成,张耗,王志琴,2007,结实期籽粒乙烯释放速率和1-氨基环丙烷-1-羧酸浓度与稻米外观品质的关系,中国水稻科学,21(1):77-83)
Yang Y.X.,Zhao Y.,Li J.K.,and Huang X.Q.,2010,Research progress in determination methods for amylose content,Shipin Kexue(Food Science),31(23):417-422(杨有仙,赵燕,李建科,黄新球,2010,直链淀粉含量测定方法研究进展,食品科学,31(23):417-422)
Zhang J.,and Ou S.Y.,2000,The nutritional value and healthy function of buckwheat,Liangshi Yu Siliao Gongye(Cereal and Feed Industry),(11):44-45(张璟,欧仕益,2000,荞麦的营养价值和保健作用,粮食与饲料工业,(11):44-45)
Zhou X.L.,Liu T.Y.,Yan B.B.,Wang H.,Xiao Y.,and Zhou Y.M.,2018,Effect of dietary supplementation of tartary buckwheat on physiological metabolism and intestinal flora in mice with high-fat diet induced dyslipidemia,Shipin Kexue(Food Science),39(1):172-177(周小理,刘泰驿,闫贝贝,王宏,肖瀛,周一鸣,2018,苦荞对高脂膳食诱导小鼠生理及肠道菌群的影响,食品科学,39(1):172-177)
Zhu X.Q.,Yang L.Q.,Zeng H.,Wu H.Q.,Liu Z.A.,Liao F.Y.,Chen Y.H.,and Huang C.F.,2015,Effects of different illumination intensity conditions on morphological and physiology characteristic of Sedum aizoon L.,Zhongnan Linye Keji Daxue Xuebao(Journal of Central South University of Forestry&Technology),35(6):98-102(朱小青,杨柳青,曾红,吴红强,刘志昂,廖飞勇,陈月华,黄琛斐,2015,不同光照强度对费菜形态和生理特性的影响,中南林业科技大学学报,35(6):98-102)
Cheng Y.G.,Wang R.,and Meng L.,2012,Effect of light intensity on the growth and vitamin C content of safflower sprouts,Beifang Yuanyi(Northern Horticulture),(22):154-156(成元刚,王锐,孟丽,2012,光照强度对红花芽菜生长和维生素C含量的影响,北方园艺,(22):154-156)
Cui H.Y.,Jin L.B.,Li B.,Dong S.T.,Liu P.,Zhao B.,and Zhang J.W.,2013,Effects of shading on dry matter accumulation and nutrient absorption of summer maize,Yingyong Shengtai Xuebao(Chinese Journal of Applied Ecology),24(11):3099-3105(崔海岩,靳立斌,李波,董树亭,刘鹏,赵斌,张吉旺,2013,遮阴对夏玉米干物质积累及养分吸收的影响,应用生态学报,24(11):3099-3105)
Dong M.H.,Zhao B.H.,Wu X.Z.,Chen T.,and Yang J.C.,2008,Difference in hormonal content and activities of key enzymes in the grains at different positions on a rice panicle during grain filling and their correlations with rice qualities,Zhongguo Nongye Kexue(Scientia Agricultura Sinica),41(2):370-380(董明辉,赵步洪,吴翔宙,陈涛,杨建昌,2008,水稻结实期不同粒位籽粒相关内源激素含量和关键酶活性的差异及其与品质的关系,中国农业科学,41(2):370-380)
Flores H.E.,and Galston A.W.,1982,Analysis of polyamines in higher plants by high performance liquid chromatography,Plant Physiol.,69(3):701-706
Gao J.,Shi J.G.,Dong S.T.,Liu P.,Zhao B.,and Zhang J.W.,2017,Effect of different light intensities on root characteristics and grain yield of summer maize(Zea mays L.),Zhongguo Nongye Kexue(Scientia Agricultura Sinica),50(11):2104-2113(高佳,史建国,董树亭,刘鹏,赵斌,张吉旺,2017,花粒期光照强度对夏玉米根系生长和产量的影响,中国农业科学,50(11):2104-2113)
He Z.P.,ed.,1993,Guidance to experiment on chemical control incrop plants,Beijing Agricultural University Press,Beijing,China,pp.60-68(何钟佩,编著,1993,农作物化学控制试验指导,北京农业大学出版社,中国,北京,pp.60-68)
Huang K.F.,Jiang J.Z.,Qin Y.L.,Yang K.,and Cao B.S.,2008,Effect of organic fertilizer and inorganic fertilizer on dietary fiber content of Zizania latifolia,Zhongguo Shucai(China Vegetables),1(4):17-20(黄凯丰,江解增,秦玉莲,杨凯,曹碚生,2008,有机肥和无机肥对茭白膳食纤维含量的影响,中国蔬菜,1(4):17-20)
Huang K.F.,Peng H.R.,Guo X.,Zhao G.,and Chen Q.F.,2013,Difference of hormonal content in two tartary buckwheat cultivars and their relations to yield and quality,Jiangsu Nongye Xuebao(Jiangsu Journal of Agricultural Sciences),29(1):28-32(黄凯丰,彭慧蓉,郭肖,赵钢,陈庆富,2013,2个苦荞品种成熟期内源激素的含量差异和品质的关系,江苏农业学报,29(1):28-32)
Huang X.Y.,Zeller F.J.,Huang K.F.,Shi T.X.,and Chen Q.F.,2014,Variation of major minerals and trace elements in seeds of tartary buckwheat(Fagopyrum tatarium Gaertn.),Genet.Resour.Crop Ev.,61(3):567-577
Liu Z.F.,Gou L.,Zhao M.,and Zhang B.J.,2011,Effects of shading on stalk morphological characteristics,rind penetra tion strength and lodging-resistance of maize,Huabei Nongxuebao(Acta Agriculturae Boreali-Sinica),26(4):91-96(刘仲发,勾玲,赵明,张保军,2011,遮阴对玉米茎秆形态特征,穿刺强度及抗倒伏能力的影响,华北农学报,26(4):91-96)
Luo X.P.,Zhu D.Y.,Huang Y.J.,Li M.F.,Yao P.F.,Gao F.,Li C.L.,and Zhao H.X.,2015,Effects of methyl jasmonate accumulation of flavonoids and related gene expression of buckwheat sprouts,Jiyinzuxue Yu Yingyong Shengwuxue(Ge nomics and Applied Biology),34(5):1040-1046(雒晓鹏,朱冬寅,黄云吉,李茂菲,姚攀锋,高飞,李成磊,赵海霞,2015,茉莉酸甲酯对芽期苦荞黄酮积累及相关基因表达的影响,基因组学与应用生物学,34(5):1040-1046)
Shi J.G.,Zhu K.L.,Cao H.Y.,Dong S.T.,Liu P.,Zhao B.,and Zhang J.W.,2015,Effect of light from flowering to maturity stage on dry matter accumulation and nutrient absorption of summer maize,Yingyong Shengtai Xuebao(Chinese Journal of Applied Ecology),26(1):46-52(史建国,朱昆仑,曹慧英,董树亭,刘鹏,赵斌,张吉旺,2015,花粒期光照对夏玉米干物质积累和养分吸收的影响,应用生态学报,26(1):46-52)
Wang Y.,Qi X.N.,Shao J.F.,Liu Z.Y.,and Li Z.X.,2008,Effects of light intensity at full growing stage on the growth and yield of different maize varieties,Jilin Nongye Daxue Xue bao(Journal of Jilin Agricultural University),30(6):769-773(王洋,齐晓宁,邵金锋,刘兆永,李泽兴,2008,光照强度对不同玉米品种生长发育和产量构成的影响,吉林农业大学学报,30(6):769-773)
Wang Z.Q.,Ye Y.X.,Yang J.C.,Yuan L.M.,Wang X.M.,and Zhu Q.S.,2004,Changes and regulation of sucrose synthase activity in grains during grain filling stage of rice,Zuowu Xuebao(Acta Agronomica Sinica),30(7):634-643(王志琴,叶玉秀,杨建昌,袁莉民,王学明,朱庆森,2004,水稻灌浆期籽粒中蔗糖合成酶活性的变化与调节,作物学报,30(7):634-643)
Yan J.,Wang D.L.,Hao J.X.,and Xie S.X.,2017,Effects of light intensity on fruit quality of rabbiteye blueberry in Guizhou,Jingjilin Yanjiu(Nonwood Forest Research),35(4):118-122(闫静,王德炉,郝家孝,谢双喜,2017,光照强度对贵州兔眼蓝莓果实品质的影响,经济林研究,35(4):118-122)
Yang J.C.,Chang E.H.,Tang C.,Zhang H.,and Wang Z.Q.,2007,Relationships of ethylene evolution rate and 1-aminocylo propane-l-carboxylic acid concentration in grains during grain filling with appearance quality of rice,Zhongguo Shuidao Kexue(Chinese Journal of Rice Science),21(1):77-83(杨建昌,常二华,唐成,张耗,王志琴,2007,结实期籽粒乙烯释放速率和1-氨基环丙烷-1-羧酸浓度与稻米外观品质的关系,中国水稻科学,21(1):77-83)
Yang Y.X.,Zhao Y.,Li J.K.,and Huang X.Q.,2010,Research progress in determination methods for amylose content,Shipin Kexue(Food Science),31(23):417-422(杨有仙,赵燕,李建科,黄新球,2010,直链淀粉含量测定方法研究进展,食品科学,31(23):417-422)
Zhang J.,and Ou S.Y.,2000,The nutritional value and healthy function of buckwheat,Liangshi Yu Siliao Gongye(Cereal and Feed Industry),(11):44-45(张璟,欧仕益,2000,荞麦的营养价值和保健作用,粮食与饲料工业,(11):44-45)
Zhou X.L.,Liu T.Y.,Yan B.B.,Wang H.,Xiao Y.,and Zhou Y.M.,2018,Effect of dietary supplementation of tartary buckwheat on physiological metabolism and intestinal flora in mice with high-fat diet induced dyslipidemia,Shipin Kexue(Food Science),39(1):172-177(周小理,刘泰驿,闫贝贝,王宏,肖瀛,周一鸣,2018,苦荞对高脂膳食诱导小鼠生理及肠道菌群的影响,食品科学,39(1):172-177)
Zhu X.Q.,Yang L.Q.,Zeng H.,Wu H.Q.,Liu Z.A.,Liao F.Y.,Chen Y.H.,and Huang C.F.,2015,Effects of different illumination intensity conditions on morphological and physiology characteristic of Sedum aizoon L.,Zhongnan Linye Keji Daxue Xuebao(Journal of Central South University of Forestry&Technology),35(6):98-102(朱小青,杨柳青,曾红,吴红强,刘志昂,廖飞勇,陈月华,黄琛斐,2015,不同光照强度对费菜形态和生理特性的影响,中南林业科技大学学报,35(6):98-102)