低浓度乙酸预处理玉米芯的工艺研究
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摘要
以脱除木质素,降解半纤维素为木糖,提高纤维素酶解得率为目的,研究了低浓度乙酸预处理玉米芯的效果,考察了乙酸质量分数、预处理温度和时间对预处理的影响。研究结果表明:质量分数5%乙酸预处理玉米芯可以脱除大部分的半纤维素和少部分木质素,预处理后的玉米芯具有较好的水解效果。低浓度乙酸预处理玉米芯最优条件为:预处理温度160℃,保温时间60 min,乙酸质量分数5%,固液比1∶8(g∶mL)。在此条件下,玉米芯固体渣回收率为53.75%,固体渣中纤维素保留率93.17%,半纤维素脱除率87.36%,木质素脱除率25.04%,预处理液中木糖质量浓度15.56 g/L。预处理后的玉米芯固体经72 h酶解,酶解得率为92.69%。
A dilute acetic acid pretreatment technology for corncob was established to remove lignin, convert hemicellulose into xylose and improve the enzymatic hydrolysis yield. The effects of acetic acid concentration, pretreatment temperature and time on pretreatment goal were investigated and the results showed that most of the hemicellulose and partial lignin in the corncob were removed by pretreating with 5% acetic acid. Moreover, the pretreated corncob was easy to be hydrolyzed. Under the optimal condition(pretreatment temperature of 160 ℃, holding time of 60 min, acetic acid concentration of 5% and solid-liquid ratio of 1∶8(g∶mL), the yield of corncob solid residue was 53.75%, the cellulose retention rate of corncob solid residue could reach 93.17%, and 87.36% of the hemicelluloses and 25.04% of the lignin were removed. Meanwhile, 15.56 g/L xylose was obtained from the pretreatment liquid. The maximum enzymatic hydrolysis yield of pretreated corncob solid could be up to 92.69% within 72 h.
A dilute acetic acid pretreatment technology for corncob was established to remove lignin, convert hemicellulose into xylose and improve the enzymatic hydrolysis yield. The effects of acetic acid concentration, pretreatment temperature and time on pretreatment goal were investigated and the results showed that most of the hemicellulose and partial lignin in the corncob were removed by pretreating with 5% acetic acid. Moreover, the pretreated corncob was easy to be hydrolyzed. Under the optimal condition(pretreatment temperature of 160 ℃, holding time of 60 min, acetic acid concentration of 5% and solid-liquid ratio of 1∶8(g∶mL), the yield of corncob solid residue was 53.75%, the cellulose retention rate of corncob solid residue could reach 93.17%, and 87.36% of the hemicelluloses and 25.04% of the lignin were removed. Meanwhile, 15.56 g/L xylose was obtained from the pretreatment liquid. The maximum enzymatic hydrolysis yield of pretreated corncob solid could be up to 92.69% within 72 h.
引文
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[12]HAO R,ZHANG Z,ZHANG R,et al. Methane production from rice straw pretreated by a mixture of acetic-propionic acid[J]. Bioresource Technology,2010,101(3):990-994.
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[14]SHAFIEI M,KARIMI K,TABERZADEH M J,et al. Pretreatment of spruce and oak by N-methylmorpholine-N-oxide(NMMO) for efficient conversion of their cellulose to ethanol[J]. Bioresource Technology,2010,101(13):4914-4918.
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[17]QING Q,HUANG M,HE Y,et al. Dilute oxalic acid pretreatment for high total sugar recovery in pretreatment and subsequent enzymatic hydrolysis[J]. Applied Biochemistry & Biotechnology,2015,177(7):1493-1507.
[18]HELLE S,CAMERON D,LAM J,et al. Effect of inhibitory compounds found in biomass hydrolysates on growth and xylose fermentation by a genetically engineered strain of S. cerevisiae[J]. Enzyme & Microbial Technology,2003,33(6):786-792.
[19]江滔,路鹏,李国学. 玉米秸秆稀酸水解时糠醛形成原因及影响因素[J]. 农业工程学报,2009,25(7):185-189. JIANG T,LU P,LI G X. Formation cause and influencing factors of furfurals during the dilute acid hydrolysis of cornstalks[J]. Transactions of the CSAE,2009,25(7):185-189.
[20]王丽娟,张书廷,吕学斌. 超低浓度乙酸预处理玉米秸秆的研究[J]. 太阳能学报,2014,35(6):931-936. WANG L J,ZHANG S T,Lü X B. Research on pretreatment of corn stover by acetic acid withultra-low concentration[J]. Acta Energiae Solaris Sinica,2014,35(6):931-936.
[21]MOMAYEZ F,KARIMI K,KARIMI S,et al. Efficient hydrolysis and ethanol production from rice straw by pretreatment with organic acids and effluent of biogas plant[J]. RSC Advances,2017,7(80):50537-50545.
[2]陈家明,余稳稳,吴晖,等. 玉米芯的营养成分分析[J]. 现代食品科技,2012,28(9):1073-1075. CHEN J M,YU W W,WU H,et al. Analysis of the nutritional components of corn cobs[J]. Modern Food Science & Technology,2012,28(9):1073-1075.
[3]WANG G S,LEE J W,ZHU J Y,et al. Dilute acid pretreatment of corncob for efficient sugar production[J]. Applied Biochemistry and Biotechnology,2011,163(5):658-668.
[4]中华人民共和国国家统计局. 国家统计局关于2017年粮食产量的公告[EB/OL].(2017-12-08)[2018-03-08]. http://www. gov. cn/xinwen/2017-12/08/content_5245284. htm.
[5]孙向阳. 不同预处理对玉米芯酶解特性和微观结构的影响[J]. 食品工业,2015,50(6):1-4. SUN X Y. Effects of different pretreatment solution properties and micro structure of corn cob enzyme[J]. Food Industry,2015,50(6):1-4.
[6]TUCKER M P,KIM K H,NEWMAN M M,et al. Effects of temperature and moisture on dilute-acid steam explosion pretreatment of corn stover and cellulase enzyme digestibility[J]. Applied Biochemistry & Biotechnology,2003,105(1/2/3):165-177.
[7]CHEN R,LEE Y Y,TORGET R. Kinetic and modeling investigation on two-stage reverse-flow reactor as applied to dilute-acid pretreatment of agricultural residues[J]. Applied Biochemistry & Biotechnology,1996,57/58(1):133-146.
[8]LLOYDT A,WYMAN C E. Combined sugar yields for dilute sulfuric acid pretreatment of corn stover followed by enzymatic hydrolysis of the remaining solids[J]. Bioresour Technol,2005,96(18):1967-1977.
[9]LIU Y,GENG Y,ZHOU Q,et al. The effect of furfural and 5-hydroxymethyl furfural on butyric acid fermentation by Clostridium tyrobutyricum[J]. Journal of Chemical Technology & Biotechnology,2017,93:849-854
[10]CHEN X,WANG X,XUE Y,et al. Tapping the bioactivity potential of residual stream from its pretreatments may be a green strategy for low-cost bioconversion of rice straw[J]. Applied Biochemistry & Biotechnology,2018,186:507-524.
[11]LI J,SHI S,ADHIKARI S,et al. Inhibition effect of aromatic aldehydes on butanol fermentation by Clostridium acetobutylicum[J]. RSC Advances,2017,7(3):1241-1250.
[12]HAO R,ZHANG Z,ZHANG R,et al. Methane production from rice straw pretreated by a mixture of acetic-propionic acid[J]. Bioresource Technology,2010,101(3):990-994.
[13]KOOTSTRA A M J,BEEFTINK H H,SCOTT E L,et al. Comparison of dilute mineral and organic acid pretreatment for enzymatic hydrolysis of wheat straw[J]. Biochemical Engineering Journal,2009,46(2):126-131.
[14]SHAFIEI M,KARIMI K,TABERZADEH M J,et al. Pretreatment of spruce and oak by N-methylmorpholine-N-oxide(NMMO) for efficient conversion of their cellulose to ethanol[J]. Bioresource Technology,2010,101(13):4914-4918.
[15]SLUITER A,HAMES B,RUIZ R,et al. Determination of structural carbohydrates and lignin in biomass:Laboratory analytical procedure(LAP)[G]. NREL/TP-510-42618.Golden:National Renewable Energy Laboratory,2012-08-03.
[16]刘洪杰,邢山川,张思思,等. 深度共熔溶剂分离提取玉米芯纤维素[J]. 河北科技大学学报,2017,38(6):548-554. LIU H J,XING S C,ZHANG S S,et al. Study on extraction of cellulose from corn cobs by deep eutectic solvents[J]. Journal of Hebei University of Science & Technology,2017,38(6):548-554.
[17]QING Q,HUANG M,HE Y,et al. Dilute oxalic acid pretreatment for high total sugar recovery in pretreatment and subsequent enzymatic hydrolysis[J]. Applied Biochemistry & Biotechnology,2015,177(7):1493-1507.
[18]HELLE S,CAMERON D,LAM J,et al. Effect of inhibitory compounds found in biomass hydrolysates on growth and xylose fermentation by a genetically engineered strain of S. cerevisiae[J]. Enzyme & Microbial Technology,2003,33(6):786-792.
[19]江滔,路鹏,李国学. 玉米秸秆稀酸水解时糠醛形成原因及影响因素[J]. 农业工程学报,2009,25(7):185-189. JIANG T,LU P,LI G X. Formation cause and influencing factors of furfurals during the dilute acid hydrolysis of cornstalks[J]. Transactions of the CSAE,2009,25(7):185-189.
[20]王丽娟,张书廷,吕学斌. 超低浓度乙酸预处理玉米秸秆的研究[J]. 太阳能学报,2014,35(6):931-936. WANG L J,ZHANG S T,Lü X B. Research on pretreatment of corn stover by acetic acid withultra-low concentration[J]. Acta Energiae Solaris Sinica,2014,35(6):931-936.
[21]MOMAYEZ F,KARIMI K,KARIMI S,et al. Efficient hydrolysis and ethanol production from rice straw by pretreatment with organic acids and effluent of biogas plant[J]. RSC Advances,2017,7(80):50537-50545.