玉米须多糖的提取纯化及结构研究
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摘要
玉米须经蒸馏水提取、离子交换、分子筛层析等手段得玉米须多糖(SMPS),结构分析研究表明SMPS的分子量约7 000 Da,其单糖组成和比例为鼠李糖∶阿拉伯糖∶木糖∶甘露糖∶半乳糖∶葡萄糖=0. 18∶0. 75∶0. 67∶1. 22∶1. 42∶6. 82; SMPS的重复单元可能是由甘露糖、半乳糖、葡萄糖构成,甘露糖主要以1→6连接存在于主链中,葡萄糖的主要的连接方式为1→3、1→4、1→6连接,木糖在主链及支链中均有存在,半乳糖主要存在于主链的末端.
One kind of stigma maydis polysaccharide( SMPS) was extracted from stigma maydis by distilled water,then purified by ion exchange chromatography and molecular sieve chromatography. The results of structural studies showed that SMPS was composed of rhamnose,arabinose,xylose,mannose,galactose and glucose( 0. 18 ∶0. 75 ∶0. 67 ∶1. 22 ∶1. 42 ∶6. 82) with apparent molecular weight of 7 × 10~3 Da. SMPS has a repeating unit composed of mannose,galactose and glucose. Mannose mainly exists as →6)-Man-( 1 → in the main chain. Glucose mainly exists as →3)-Glc-( 1 →,→ 4)-Glc-( 1 → and → 6)-Glc-( 1 → in the pdysaccharide. Xylose exists in both backbone and branch chain. Galactose mainly exists at the end of the main chain.
One kind of stigma maydis polysaccharide( SMPS) was extracted from stigma maydis by distilled water,then purified by ion exchange chromatography and molecular sieve chromatography. The results of structural studies showed that SMPS was composed of rhamnose,arabinose,xylose,mannose,galactose and glucose( 0. 18 ∶0. 75 ∶0. 67 ∶1. 22 ∶1. 42 ∶6. 82) with apparent molecular weight of 7 × 10~3 Da. SMPS has a repeating unit composed of mannose,galactose and glucose. Mannose mainly exists as →6)-Man-( 1 → in the main chain. Glucose mainly exists as →3)-Glc-( 1 →,→ 4)-Glc-( 1 → and → 6)-Glc-( 1 → in the pdysaccharide. Xylose exists in both backbone and branch chain. Galactose mainly exists at the end of the main chain.
引文
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[2]江苏新医学院.中药大辞典(上册)[M].北京:人民卫生出版社,1980:555.
[3]刘娟,韩晓强,姜博.玉米须多糖治疗糖尿病作用研究[J].时珍国医国药,2006,17(8):1441-1442.
[4]周鸿立,张艳,金海甲,等.玉米须多糖药理作用及提取纯化研究进展[J].吉林化工学院学报,2009,26(1):23-26.
[5]周鸿立,张英珍,郭志红.三氯乙酸-酶法脱玉米须多糖中蛋白质工艺的优化[J].食品研究与开发,2015,36(3):63-66.
[6]张逸,王旺,蔡寅,等.苦瓜多糖的纯化、结构解析及其免疫调节和抗肿瘤活性研究[J].南京中医药大学学报,2017,33(1):33-39.
[7]李雅双,刘杰,包瑛,等.芜菁水溶性多糖的结构分析[J].食品与发酵工业,2015,41(8):175-180.
[8]曹荣安,徐秀丽,苗家尉,等.龙胆多糖的结构表征及其体外免疫活性[J].食品科学,2017,38(11):168-173.
[9]NIU Y,WANG H,XIE Z,et al.Structural analysis and bioactivity of a polysaccharide from the roots of Astragalus membranaceus(Fisch)Bge.var.mongolicus(Bge.)Hsiao[J].Food Chem,2011,128(3):620-626.
[10]龙旭,孟庆华,张拴,等.超临界流体萃取技术在中药提取中的应用[J].科技展望,2016,26(32):110.
[11]郭赛,张雨婷,张莉,等.超高压技术中药领域研究进展[J].广东化工,2017,44(1):53-54.
[12]粟敏,陈琳,龙昱,等.离子液体-微波辅助提取多花黄精多糖工艺研究[J].中药材,2016,39(9):2075-2077.
[13]LI S,CAO X.Extraction of tea polysaccharides(TPS)using anionic reverse micellar system[J].Sep Purif Technol,2014,122(122):306-314.
[14]汤鲁宏,丁霄霖,尤丽芬,等.玉米须生物活性成分的初步研究:玉米须多糖及其免疫增强作用[J].无锡轻工大学学报,1995,14(4):319-324.