热灭活对弓形虫IgM抗体检测影响的临床研究
|
摘要
目的评估56℃30 min热灭活对弓形虫IgM抗体磁微粒全自动化学发光检测的影响。方法将63例孕妇的惰性分离胶和促凝剂抗凝的血浆分为3组:(1)弓形虫IgM抗体阳性标本;(2)在弓形虫IgM抗体弱阳性(灰区)标本;(3)弓形虫IgM抗体阴性标本,对全部标本56℃30 min热灭活,并用磁微粒全自动化学发光测定仪对热灭活样本前后弓形虫IgM抗体进行定量检测分析。结果弓形虫IgM抗体阳性标本定量结果显著降低,弱阳性(灰区)标本结果显著降低并变为阴性,阴性标本检测值显著降低但仍为阴性结果。结论 56℃30 min热灭活显著降低弓形虫IgM抗体样品磁微粒全自动化学发光检测结果,在弓形虫IgM抗体日常检测过程中应避免样本的56℃30 min热灭活处理过程。
Objective This study is to evaluate the effect of 56 ℃ 30 min thermal inactivation on the results of IgM antibodies of toxoplasma gondii. Methods The 63 pregnant women's plasma were divided into three groups, including the positive specimens of toxoplasma gondii IgM antibody, the weak positive(gray area) specimens of Toxoplasma gondii IgM antibody, and the IgM antibody negative specimens of toxoplasma gondii. The IgM antibody of toxoplasma gondii was then quantified by magnetic particle automatic chemiluminescence analyzer after the 56 ℃ 30 min thermal inactivation. Results The quantitative results of positive samples of the IgM antibody of toxoplasma gondii significantly decreased, and the results of weak positive(grey area) samples decreased significantly and became negative. The negative samples were still negative. Conclusion Thermal inactivation at 56 ℃ 30 min significantly reduced the results of IgM antibody of toxoplasma gondii, and this thermal inactivation process should be avoided in the routine detection of IgM antibody test of toxoplasma gondii.
Objective This study is to evaluate the effect of 56 ℃ 30 min thermal inactivation on the results of IgM antibodies of toxoplasma gondii. Methods The 63 pregnant women's plasma were divided into three groups, including the positive specimens of toxoplasma gondii IgM antibody, the weak positive(gray area) specimens of Toxoplasma gondii IgM antibody, and the IgM antibody negative specimens of toxoplasma gondii. The IgM antibody of toxoplasma gondii was then quantified by magnetic particle automatic chemiluminescence analyzer after the 56 ℃ 30 min thermal inactivation. Results The quantitative results of positive samples of the IgM antibody of toxoplasma gondii significantly decreased, and the results of weak positive(grey area) samples decreased significantly and became negative. The negative samples were still negative. Conclusion Thermal inactivation at 56 ℃ 30 min significantly reduced the results of IgM antibody of toxoplasma gondii, and this thermal inactivation process should be avoided in the routine detection of IgM antibody test of toxoplasma gondii.
引文
[1] Srivastava SK,Ruigrok VJ,Thompson NJ,et al.16 kDa heat shock protein from heat-inactivated Mycobacterium tuberculosis is a homodimer-suitability for diagnostic applications with specific llama VHH monoclonals[J].PLoS ONE,2013,8(5):e64040.
[2] Fipps DR,Damato JJ,Burke DS.Effects of heat inactivation on HIV antibody screening and confirmatory test systems[J].Diagn Microbiol Infect Dis,1988,10(2):103-107.
[3] Riley AA,Klingman L,Brier ME,et al.In vivo heat inactivation of serum can distinguish false positive cross matches in renal transplants[J].Int J Artif Organs,2016,39(2):63-67.
[4] Pokrass MJ,Liu MF,Lindorfer MA,et al.Activation of complement by monoclonal antibodies that target cell-associated beta(2)-microglobulin:implications for cancer immunotherapy[J].Mol Immunol,2013,56(4):549-560.
[5] Pasnik DJ,Evans JJ,Klesius PH.Specific serum antibody responses in channel catfish (Ictalurus punctatus) provide limited protection against Streptococcus ictaluri challenge[J].Vet Immunol Immunopathol,2011,144(1):144-146.
[6] Samoylova TI,Norris MD,Samoylov AM,et al.Infective and inactivated filamentous phage as carriers for immunogenic peptides[J].J Virol Methods,2012,183(1):63-68.
[7] Gayán E,Serrano MJ,álvarez I,et al.Modeling optimal process conditions for UV-heat inactivation of foodborne pathogens in liquid foods[J].Food Microbiol,2016,60(5):13-20.
[8] Imagawa T,Sugiyama R,Shiota T,et al.Evaluation of heating conditions for inactivation of hepatitis E virus genotypes 3 and 4[J].J Food Prot,2018,81(6):947-952.
[9] Patterson EI,Warmbrod KL,Bouyer DH,et al.Evaluation of the inactivation of Venezuelan equine encephalitis virus by several common methods[J].J Virol Methods,2018,254(4):31-34.
[10] Brenier-Pinchart MP,Fricker-Hidalgo H,Dard C,et al.Impact of heat-inactivation on anti-Toxoplasma IgM antibody levels[J].Clin Chem Lab Med,2017,55(12):291-293.
[11] Shambaugh C,Azshirvani S,Yu L,et al.Development of a high-throughput respiratory syncytial virus fluorescent focus-based microneutralization assay[J].Clin Vaccine Immunol,2017,24(12):11-16.
[12] Yu L,Esser MT,Falloon J,et al.Generalized ROC methods for immunogenicity data analysis of vaccine phase I studies in a seropositive population[J].Hum Vaccin Immunother,2018,18(2):1-29.
[13] Badman SG,Causer LM,Guy R,et al.A reliable and easy to transport quality control method for chlamydia and gonorrhoea molecular point of care testing[J].Pathology,2018,50(3):317-321.
[14] Scantlebury CE,Pinchbeck GL,Loughnane P,et al.Development and evaluation of a molecular diagnostic method for rapid detection of histoplasma capsulatum var.farciminosum,the causative agent of epizootic lymphangitis,in equine clinical samples[J].J Clin Microbiol,2016,54(12):2990-2999.
[15] Blumel J,Musso D,Teitz S,et al.Inactivation and removal of Zika virus during manufacture of plasma-derived medicinal products[J].Transfusion,2017,57(3):790-796.
[16] Pruller S,Turni C,Blackall PJ,et al.Towards a standardized method for broth microdilution susceptibility testing of haemophilus parasuis[J].J Clin Microbiol,2017,55(1):264-273.
[2] Fipps DR,Damato JJ,Burke DS.Effects of heat inactivation on HIV antibody screening and confirmatory test systems[J].Diagn Microbiol Infect Dis,1988,10(2):103-107.
[3] Riley AA,Klingman L,Brier ME,et al.In vivo heat inactivation of serum can distinguish false positive cross matches in renal transplants[J].Int J Artif Organs,2016,39(2):63-67.
[4] Pokrass MJ,Liu MF,Lindorfer MA,et al.Activation of complement by monoclonal antibodies that target cell-associated beta(2)-microglobulin:implications for cancer immunotherapy[J].Mol Immunol,2013,56(4):549-560.
[5] Pasnik DJ,Evans JJ,Klesius PH.Specific serum antibody responses in channel catfish (Ictalurus punctatus) provide limited protection against Streptococcus ictaluri challenge[J].Vet Immunol Immunopathol,2011,144(1):144-146.
[6] Samoylova TI,Norris MD,Samoylov AM,et al.Infective and inactivated filamentous phage as carriers for immunogenic peptides[J].J Virol Methods,2012,183(1):63-68.
[7] Gayán E,Serrano MJ,álvarez I,et al.Modeling optimal process conditions for UV-heat inactivation of foodborne pathogens in liquid foods[J].Food Microbiol,2016,60(5):13-20.
[8] Imagawa T,Sugiyama R,Shiota T,et al.Evaluation of heating conditions for inactivation of hepatitis E virus genotypes 3 and 4[J].J Food Prot,2018,81(6):947-952.
[9] Patterson EI,Warmbrod KL,Bouyer DH,et al.Evaluation of the inactivation of Venezuelan equine encephalitis virus by several common methods[J].J Virol Methods,2018,254(4):31-34.
[10] Brenier-Pinchart MP,Fricker-Hidalgo H,Dard C,et al.Impact of heat-inactivation on anti-Toxoplasma IgM antibody levels[J].Clin Chem Lab Med,2017,55(12):291-293.
[11] Shambaugh C,Azshirvani S,Yu L,et al.Development of a high-throughput respiratory syncytial virus fluorescent focus-based microneutralization assay[J].Clin Vaccine Immunol,2017,24(12):11-16.
[12] Yu L,Esser MT,Falloon J,et al.Generalized ROC methods for immunogenicity data analysis of vaccine phase I studies in a seropositive population[J].Hum Vaccin Immunother,2018,18(2):1-29.
[13] Badman SG,Causer LM,Guy R,et al.A reliable and easy to transport quality control method for chlamydia and gonorrhoea molecular point of care testing[J].Pathology,2018,50(3):317-321.
[14] Scantlebury CE,Pinchbeck GL,Loughnane P,et al.Development and evaluation of a molecular diagnostic method for rapid detection of histoplasma capsulatum var.farciminosum,the causative agent of epizootic lymphangitis,in equine clinical samples[J].J Clin Microbiol,2016,54(12):2990-2999.
[15] Blumel J,Musso D,Teitz S,et al.Inactivation and removal of Zika virus during manufacture of plasma-derived medicinal products[J].Transfusion,2017,57(3):790-796.
[16] Pruller S,Turni C,Blackall PJ,et al.Towards a standardized method for broth microdilution susceptibility testing of haemophilus parasuis[J].J Clin Microbiol,2017,55(1):264-273.