贵州艾纳香的主要成分质量测定
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摘要
为贵州艾纳香药材的质量标准的修改和制定提供基础数据,按照《中国药典》2015版中附录及相关方法,对艾纳香药材进行水分、总灰分和酸不溶性灰分鉴别;采用薄层色谱法(TLC)和高效液相色谱法(HPLC)对其进行定性定量分析。结果表明:不同产区艾纳香药材的水分含量不超过14%,总灰分不高于14%,酸不溶性灰分不高于3%;药材提取液TLC斑点清晰,分离度好;艾纳香素含量在6.375～38.25μg/mL,线性范围良好,平均回收率97.93%,RSD为2.63%(n=6)。结论:艾纳香的质量测定稳定、准确、可靠。
To provide basic data for the revision and formulation of the quality standards of Blumea balsamifera medicinal materials of Guizhou,the moisture,total ash and acid-insoluble ash of B.balsamifera were identified according to the appendix of the Chinese Pharmacopoeia 2015 edition and related methods.The qualitative and quantitative analysis was carried out by thin layer chromatography(TLC)and high performance liquid chromatography(HPLC).Results:The water content of B.balsamifera materials in different producing areas was not more than 14%,the total ash content was not higher than 14%,the acid insoluble ash content was not higher than 3%.The TLC spots of the medicinal material extract were clear and the separation degree was good;The content of Blumeatin was 6.375-38.25μg/mL,the linear range was good,the average recovery was 97.93%,and the RSD was 2.63%(n=6).Conclusion:The quality of B.balsamiferais stable,accurate and reliable.
To provide basic data for the revision and formulation of the quality standards of Blumea balsamifera medicinal materials of Guizhou,the moisture,total ash and acid-insoluble ash of B.balsamifera were identified according to the appendix of the Chinese Pharmacopoeia 2015 edition and related methods.The qualitative and quantitative analysis was carried out by thin layer chromatography(TLC)and high performance liquid chromatography(HPLC).Results:The water content of B.balsamifera materials in different producing areas was not more than 14%,the total ash content was not higher than 14%,the acid insoluble ash content was not higher than 3%.The TLC spots of the medicinal material extract were clear and the separation degree was good;The content of Blumeatin was 6.375-38.25μg/mL,the linear range was good,the average recovery was 97.93%,and the RSD was 2.63%(n=6).Conclusion:The quality of B.balsamiferais stable,accurate and reliable.
引文
[1]贵州省药品监督管理局.贵州省中药、民族药材质量标准[S].贵阳:贵州科技出版社,2007:308-309.
[2]江苏医学院.中药大辞典[M].上海:上海科学技术出版社,1986.
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[4]国家药典委员会.中华人民共和国药典:四部[S].北京:中国医药科技出版社,2015:57-204.
[5]严启新,谭道鹏,康晖,等.艾纳香中的黄酮类化学成分[J].中国实验方剂学杂志,2012,18(5):86-89.
[6]郜舒蕊,赵志刚,侯俊玲,等.均匀设计法优化丹参中4种活性成分的超声提取工艺[J].中国实验方剂学杂志,2014,20(10):8-12.
[7]谢伟杰,张永萍,徐剑,等.正交设计联用Box-Behnken效应面法优选复方石斛清肺散提取工艺[J].中成药,2015,37(8):1683-1688.
[8]庞玉新,袁蕾,王中洋,等.艾纳香不同部位提取物的抗氧化活性及其对酪氨酸酶的抑制作用[J].中国实验方剂学杂志,2014,20(18):4-8.
[9]姜建萍,陈晨,蓝仁青,等.滇桂艾纳香不同提取物凝血作用的比较研究[J].中国实验方剂学杂志,2010,16(1):104-106.
[10]夏嬿,李祥,陈建伟.艾纳香叶废弃物镇痛、抗炎、止血活性的初步研究[J].天然产物研究与开发,2015,27(6):1086-1091.
[2]江苏医学院.中药大辞典[M].上海:上海科学技术出版社,1986.
[3]郝小燕,余珍,丁智慧.黔产艾纳香挥发油化学成分研究[J].贵阳医学院学报,2000,25(2):121-122.
[4]国家药典委员会.中华人民共和国药典:四部[S].北京:中国医药科技出版社,2015:57-204.
[5]严启新,谭道鹏,康晖,等.艾纳香中的黄酮类化学成分[J].中国实验方剂学杂志,2012,18(5):86-89.
[6]郜舒蕊,赵志刚,侯俊玲,等.均匀设计法优化丹参中4种活性成分的超声提取工艺[J].中国实验方剂学杂志,2014,20(10):8-12.
[7]谢伟杰,张永萍,徐剑,等.正交设计联用Box-Behnken效应面法优选复方石斛清肺散提取工艺[J].中成药,2015,37(8):1683-1688.
[8]庞玉新,袁蕾,王中洋,等.艾纳香不同部位提取物的抗氧化活性及其对酪氨酸酶的抑制作用[J].中国实验方剂学杂志,2014,20(18):4-8.
[9]姜建萍,陈晨,蓝仁青,等.滇桂艾纳香不同提取物凝血作用的比较研究[J].中国实验方剂学杂志,2010,16(1):104-106.
[10]夏嬿,李祥,陈建伟.艾纳香叶废弃物镇痛、抗炎、止血活性的初步研究[J].天然产物研究与开发,2015,27(6):1086-1091.