摘要
目的:研究艾纳香非挥发性部位UPLC指纹图谱与抗炎药效之间的相关性,为明确艾纳香抗炎药效物质基础提供实验依据。方法:采用UPLC建立12批艾纳香非挥发性部位的指纹图谱,运用UPLC-Q-TOF-MS对艾纳香非挥发性部位的共有指纹峰进行指认,通过二甲苯致小鼠耳廓肿胀炎症模型获取相应的药效数据,通过灰色关联分析建立其谱效关系。结果:UPLC指纹图谱显示艾纳香非挥发性部位有14个共有峰,与抗炎药效的关联度处于0.717 1~0.550 5,各特征峰所代表的化学成分对其抗炎药效贡献的大小顺序为3号峰> 9号峰> 4号峰> 11号峰> 2号峰> 1号峰> 14号峰> 7号峰> 6号峰> 5号峰> 12号峰> 8号峰> 10号峰> 13号峰,并指认了其中9个共有峰,对抗炎药效贡献较大的前2个共有峰(3号和9号)对应的化学成分分别为3-O-咖啡酰基奎宁酸和3,5-O-二咖啡酰基奎宁酸。结论:通过谱效关系研究获得了艾纳香非挥发性部位的抗炎药效物质群,其抗炎药效是多种成分共同作用的结果,明确咖啡酰基奎宁酸类化合物为艾纳香非挥发性部位发挥抗炎药效的主要物质基础。
Objective:To study the correlation between UPLC fingerprint of anti-inflammatory effect of active components from nonvolatile fraction of Blumea balsamifera,and to provide the basis for clarifying the antiinflammatory material basis of B.balsamifera.Method:UPLC was used to establish fingerprint of nonvolatile fraction of 12 batches of B.balsamifera and their common fingerprint peaks were identified by UPLC-Q-TOF-MS.The corresponding pharmacodynamic data were obtained by auricle swelling and inflammation model mice induced by xylene,and spectrum-effect relationship was established by gray correlation analysis.Result:A total of 14 common peaks in nonvolatile fraction of B.balsamifera were established by UPLC fingerprint and 9 common peaks of them were identified.The correlation between UPLC fingerprint and the anti-inflammatory activity was from0.717 1 to 0.550 5.The contribution of chemical compositions represented by each characteristic peak to the antiinflammatory efficacy was in the order of peak 3 > peak 9 > peak 4 > peak 11 > peak 2 > peak 1 > peak 14 > peak7 > peak 6 > peak 5 > peak 12 > peak 8 > peak 10 > peak 13,and the top two peaks with strong contribution to anti-inflammatory effect were peak 3 and peak 9,they were 3-O-caffeoylquinic acid and 3,5-di-O-caffeoylquinic acid identified by contrast reference substances,respectively.Conclusion:The active substances in nonvolatile fraction of B.balsamifera are obtained through the study on the relationship between spectrum and efficiency,and the anti-inflammatory efficacy of the nonvolatile fraction is the result of combination of various components.It is clear that the caffeoylquinic acid derivates act as predominant anti-inflammatory active substance of nonvolatile fraction of B.balsamifera.
引文
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