5种光合模型下巨柏叶绿素荧光—快速光响应曲线拟合的比较
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摘要
以巨柏为研究材料,用Li-6400-40光合仪荧光叶室,测定4种基质苗木的叶绿素荧光—快速光响应曲线。分别用5种模型进行拟合比较主要荧光参数与实测值的接近程度,筛选最佳的巨柏叶绿素荧光—快速光响应模型。结果表明,直角双曲线模型、非直角双曲线模型和单指数方程无法拟合巨柏的PSⅡ动力学下调时的曲线,无法直接求取巨柏的饱和光强,且最大电子传递效率与实测值偏差较大;双指数方程和双曲线修正模型都可以拟合巨柏的主要荧光参数,但双指数方程给出的最大电子传递效率低于实测值;饱和光强高于实测值;采用双曲线修正模型拟合4种基质的巨柏快速光曲线的最大电子传递效率和饱和光强几乎完全与实测值符合,均达到显著水平,同时从均方根误差、平均相对误差绝对值和赤池信息量准则模型评价参数来看,双曲线修正模型的3个参数均小于其它模型,拟合程度最佳。因此双曲线修正模型为拟合巨柏叶绿素荧光—快速光曲线的最佳模型。
As a research objective, Cupressus gigantean was used to determine the chlorophyll fluorescence-rapid light curve of four substrates by Fluorescence Leaf Chamber of Li-6400-40 photosynthetic instrument. Five models were used to compare the proximity between the main fluorescence parameters and the measured values in order to filter the best chlorophyll fluorescence-rapid response model of the Cupressus gigantean. The results showed that rectangular hyperbolic model, non-rectangular hyperbolic model and single exponential equation could not fit the curves of dynamic downregulation of PSII and could not directly calculate the saturation light intensity of Cupressus gigantean, and the maximum electron transport rate was higher than the measured values. Double exponential equation and modified hyperbolic model could fit the main fluorescence parameters of Cupressus gigantean, but the maximum electron transport rate given by the double exponential equation was lower than the measured value, and the saturated light intensity was higher than the measured value. The maximum electron transfer efficiency and saturated light intensity obtained from hyperbolic correction model for the fast light curves of Cupressus gigantean on four substrates were almost completely in agreement with the measured values, and both of them reached significant levels. At the same time, according to the error of root mean square, the absolute value of relative error and the evaluation parameters of model of Akaike information criterion, the three parameters of modified hyperbolic models were smaller than other models, in addition, the fitting degree was the highest. Therefore, the modified rectangular hyperbolic model was the best model to fit the chlorophyll fluorescence-rapid light curve of Cupressus gigantean.
As a research objective, Cupressus gigantean was used to determine the chlorophyll fluorescence-rapid light curve of four substrates by Fluorescence Leaf Chamber of Li-6400-40 photosynthetic instrument. Five models were used to compare the proximity between the main fluorescence parameters and the measured values in order to filter the best chlorophyll fluorescence-rapid response model of the Cupressus gigantean. The results showed that rectangular hyperbolic model, non-rectangular hyperbolic model and single exponential equation could not fit the curves of dynamic downregulation of PSII and could not directly calculate the saturation light intensity of Cupressus gigantean, and the maximum electron transport rate was higher than the measured values. Double exponential equation and modified hyperbolic model could fit the main fluorescence parameters of Cupressus gigantean, but the maximum electron transport rate given by the double exponential equation was lower than the measured value, and the saturated light intensity was higher than the measured value. The maximum electron transfer efficiency and saturated light intensity obtained from hyperbolic correction model for the fast light curves of Cupressus gigantean on four substrates were almost completely in agreement with the measured values, and both of them reached significant levels. At the same time, according to the error of root mean square, the absolute value of relative error and the evaluation parameters of model of Akaike information criterion, the three parameters of modified hyperbolic models were smaller than other models, in addition, the fitting degree was the highest. Therefore, the modified rectangular hyperbolic model was the best model to fit the chlorophyll fluorescence-rapid light curve of Cupressus gigantean.
引文
[1]胡文海,张斯斯,肖宜安等.两种杜鹃花属植物对长期遮阴后全光照环境的生理响应及其光保护机制[J].植物生态学报,2015,39(11):1093-1100.
[2]RALPH P J,GADEMANN R.Rapid light curves:Apowerful tool to assess photosynthetic activity[J].Aquatic Botany,2005,82(03):222-237.
[3]Baly E C.The kinetics of photosynthesis[J].Proceedings of the Royal Society(Biological sciences),1935,117(104):218-239.
[4]Thornley J H M.Mathematical models in plant physiology[M].academic press,1976:86-110.
[5]Platt T,Gallegos C L,Harrison W G.Photo inhibition of photosynthesis in natural assemblages of marine phytoplankton[J].Publications-Astronomical Society of Japan,1980,57(57):341-345.
[6]Harrison W G,Platt T.Photosynthesis-irradiance relation-ship in polar and temperate phytoplankton populations[J].Polar Biology,1986,5:153-164.
[7]Ye Zi Piao,Yu Qiang.A coupled model of stomata conductance and photosynthesis for winter wheat[J].Photosynthesis,2008,46(46):637-640.
[8]朱弘,温国胜.3种模型对毛竹快速生长期冠层叶片叶绿素荧光-快速光响应曲线(RLCS)拟合的比较[J].福建农林大学学报(自然科学版),2017,46(06):659-664.
[9]周可金,肖文娜,官春云.不同油菜品种角果光合特性及叶绿素荧光参数的差异[J].中国油料作物学报,2009,31(03):316-321.
[10]刘强,李凤日,谢龙飞.人工长白落叶松冠层光合作用-光响应曲线最优模型[J].应用生态学报,2016,27(08):2420-2428.
[11]辛福梅,任世强,普布次仁.不同处理方法对巨柏种子萌发的影响[J].种子,2017,36(11):1-4.
[12]李永霞,张豪,徐杨.藏东南巨柏种子发芽及幼苗生长特性研究[J].种子,2017,36(04):91-93.
[13]德庆措姆,潘刚,霍美丽.西藏林芝地区巨柏育苗与造林试验[J].林业调查规划,2008,33(06):139-141.
[14]罗建,王景升,罗大庆,等.巨柏群落特征的研究[J].林业科学研究,2006(03):295-300.
[15]王景升,郑维列,潘刚.巨柏种子活力与濒危的关系[J].林业科学,2005(04):37-41.
[16]张国强,罗大庆,王景升.西藏濒危植物巨柏的生物学与生态学特性研究[J].林业科技,2006(02):1-5.
[17]叶子飘,王建林.植物光合-光响应模型的比较分析[J].井冈山学院学报:自然科学,2009,30(04):9-13.
[18]Chen Z Y,Peng Z S,Yang J,et al.A mathematical model for describing light-response curves in Nicotianata bacum L[J].Photosynthetica,2011,49:467-471.
[19]Akaike H.A new look at the statistical model identification[J].IEEE Transactions on Automatic Control,1974,19(06):716-723.
[20]刘泽彬,程瑞梅,肖文发等.不同淹水时间下中华蚊母树光响应特征及其模型比较[J].应用生态学报,2015,26(04):1083-1090.
[21]钱莲文,张新时,杨智杰等.几种光合作用光响应典型模型的比较研究[J].武汉植物学研究,2009,27(02):197-203.
[22]叶子飘,李进省.光合作用对光响应的直角双曲线修正模型和非直角双曲线模型的对比研究[J].井冈山大学学报:(自然科学版),2010,31(03):38-44.
[23]廖小锋,刘济明等.野生小蓬竹的光合光响应曲线及其模型拟合[J].中南林业科技大学学报,2012,32(03):124-128.
[24]叶子飘,高峻.光响应和CO2响应新模型在丹参中的应用[J].西北农林科技大学学报(自然科学版),2009,37(01):129-134.
[25]王锦旗,宋玉芝,薛艳.紫外辐射对菹草(Potamogeton crispus)成株快速光响应曲线的影响[J].湖泊科学,2015,27(03):451-458
[2]RALPH P J,GADEMANN R.Rapid light curves:Apowerful tool to assess photosynthetic activity[J].Aquatic Botany,2005,82(03):222-237.
[3]Baly E C.The kinetics of photosynthesis[J].Proceedings of the Royal Society(Biological sciences),1935,117(104):218-239.
[4]Thornley J H M.Mathematical models in plant physiology[M].academic press,1976:86-110.
[5]Platt T,Gallegos C L,Harrison W G.Photo inhibition of photosynthesis in natural assemblages of marine phytoplankton[J].Publications-Astronomical Society of Japan,1980,57(57):341-345.
[6]Harrison W G,Platt T.Photosynthesis-irradiance relation-ship in polar and temperate phytoplankton populations[J].Polar Biology,1986,5:153-164.
[7]Ye Zi Piao,Yu Qiang.A coupled model of stomata conductance and photosynthesis for winter wheat[J].Photosynthesis,2008,46(46):637-640.
[8]朱弘,温国胜.3种模型对毛竹快速生长期冠层叶片叶绿素荧光-快速光响应曲线(RLCS)拟合的比较[J].福建农林大学学报(自然科学版),2017,46(06):659-664.
[9]周可金,肖文娜,官春云.不同油菜品种角果光合特性及叶绿素荧光参数的差异[J].中国油料作物学报,2009,31(03):316-321.
[10]刘强,李凤日,谢龙飞.人工长白落叶松冠层光合作用-光响应曲线最优模型[J].应用生态学报,2016,27(08):2420-2428.
[11]辛福梅,任世强,普布次仁.不同处理方法对巨柏种子萌发的影响[J].种子,2017,36(11):1-4.
[12]李永霞,张豪,徐杨.藏东南巨柏种子发芽及幼苗生长特性研究[J].种子,2017,36(04):91-93.
[13]德庆措姆,潘刚,霍美丽.西藏林芝地区巨柏育苗与造林试验[J].林业调查规划,2008,33(06):139-141.
[14]罗建,王景升,罗大庆,等.巨柏群落特征的研究[J].林业科学研究,2006(03):295-300.
[15]王景升,郑维列,潘刚.巨柏种子活力与濒危的关系[J].林业科学,2005(04):37-41.
[16]张国强,罗大庆,王景升.西藏濒危植物巨柏的生物学与生态学特性研究[J].林业科技,2006(02):1-5.
[17]叶子飘,王建林.植物光合-光响应模型的比较分析[J].井冈山学院学报:自然科学,2009,30(04):9-13.
[18]Chen Z Y,Peng Z S,Yang J,et al.A mathematical model for describing light-response curves in Nicotianata bacum L[J].Photosynthetica,2011,49:467-471.
[19]Akaike H.A new look at the statistical model identification[J].IEEE Transactions on Automatic Control,1974,19(06):716-723.
[20]刘泽彬,程瑞梅,肖文发等.不同淹水时间下中华蚊母树光响应特征及其模型比较[J].应用生态学报,2015,26(04):1083-1090.
[21]钱莲文,张新时,杨智杰等.几种光合作用光响应典型模型的比较研究[J].武汉植物学研究,2009,27(02):197-203.
[22]叶子飘,李进省.光合作用对光响应的直角双曲线修正模型和非直角双曲线模型的对比研究[J].井冈山大学学报:(自然科学版),2010,31(03):38-44.
[23]廖小锋,刘济明等.野生小蓬竹的光合光响应曲线及其模型拟合[J].中南林业科技大学学报,2012,32(03):124-128.
[24]叶子飘,高峻.光响应和CO2响应新模型在丹参中的应用[J].西北农林科技大学学报(自然科学版),2009,37(01):129-134.
[25]王锦旗,宋玉芝,薛艳.紫外辐射对菹草(Potamogeton crispus)成株快速光响应曲线的影响[J].湖泊科学,2015,27(03):451-458