双氢青蒿素对人肺腺癌细胞A549增殖和凋亡的影响
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摘要
目的探讨双氢青蒿素对人肺腺癌细胞A549凋亡及增殖影响。方法将对数生长期人肺腺癌细胞A549随机分为实验组和对照组,实验组在细胞培养液中加入500nmol/L双氢青蒿素,对照组加入二甲基亚砜,每组细胞均培养72h,采用四甲基偶氮唑盐法检测细胞增殖能力,计算增殖抑制率,采用试剂盒检测细胞半胱氨酸蛋白酶3(Caspase3)活性,采用免疫蛋白印迹法检测Bcl-2、Bax、Akt蛋白表达水平。结果实验组细胞增殖抑制率和Caspase3活性高于对照组(P<0.05);实验组Bcl-2、Akt蛋白水平低于对照组(P<0.05),Bax蛋白水平高于对照组(P<0.05)。结论双氢青蒿素可抑制人肺腺癌细胞A549增殖能力,可能通过P13K/Akt信号传导通路促进A549细胞凋亡。
Objective To investigate the effects of dihydroartemisinin(DHA)on apoptosis and proliferation of human lung adenocarcinoma cell line A549.Methods Human lung adenocarcinoma A549 cell line in logarithmic growth phase were randomly divided into experimental group and control group.DHA with concentration of 500 nmol/L was added into cell culture in experimental group,and dimethyl sulfoxide was added in control group.Both groups were cultured for 72 h.The cell proliferation was detected by using tetramethylazozolium salt method,the activity of Caspase3 was detected by using kits,and the levels of Bcl-2,Bax and Akt protein were detected by using western blot method.Results The cell proliferation inhibition rate and Caspase3 activity in experimental group were significantly higher than control group(P<0.05).The levels of Bcl-2 and Akt protein in experimental group were significantly lower than control group(P<0.05),but level of Bax protein was significantly higher than control group(P<0.05).Conclusion DHA could inhibit the proliferation of human lung adenocarcinoma cell line A549 and promote apoptosis of A549 cells through P13 K/Akt signaling pathway.
Objective To investigate the effects of dihydroartemisinin(DHA)on apoptosis and proliferation of human lung adenocarcinoma cell line A549.Methods Human lung adenocarcinoma A549 cell line in logarithmic growth phase were randomly divided into experimental group and control group.DHA with concentration of 500 nmol/L was added into cell culture in experimental group,and dimethyl sulfoxide was added in control group.Both groups were cultured for 72 h.The cell proliferation was detected by using tetramethylazozolium salt method,the activity of Caspase3 was detected by using kits,and the levels of Bcl-2,Bax and Akt protein were detected by using western blot method.Results The cell proliferation inhibition rate and Caspase3 activity in experimental group were significantly higher than control group(P<0.05).The levels of Bcl-2 and Akt protein in experimental group were significantly lower than control group(P<0.05),but level of Bax protein was significantly higher than control group(P<0.05).Conclusion DHA could inhibit the proliferation of human lung adenocarcinoma cell line A549 and promote apoptosis of A549 cells through P13 K/Akt signaling pathway.
引文
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[9]杨华,谭先杰,郎景和,等.青蒿素衍生物对宫颈癌HeLa细胞体外增殖及凋亡能力的影响及其分子机制研究[J].现代妇产科进展,2017,26(12):881-884.
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[2]朱文赫,许娜,徐俊杰,等.双氢青蒿素对人胰腺癌细胞系增殖和凋亡的影响[J].解剖学报,2018,49(1):70-74.
[3]刘洪伟,梅基雄.双氢青蒿素通过Wnt/β-catenin信号通路抑制胃癌细胞的增殖和侵袭[J].第三军医大学学报,2018,40(5):387-394.
[4]周国运,王熙,曹干生,等.双氢青蒿素对肺癌细胞增殖的抑制作用[J].中国老年学杂志,2017,37(8):1874-1876.
[5]皮雳,徐忠烨,程远.双氢青蒿素通过下调NICD-1抑制胶质瘤生长[J].第三军医大学学报,2017,9(14):1445-1451.
[6]张雷,程龙球,周兆,等.双氢青蒿素对Raji细胞放射敏感性的影响[J].中国应用生理学杂志,2017,33(5):385-390.
[7]李亚巍,张巍,许娜,等.双氢青蒿素通过调节凋亡相关蛋白的表达及活性氧的产生而抑制胰腺癌JF-305细胞的增殖[J].中国中药杂志,2017,42(15):3026-3030.
[8]吴艳林,蔡政,张明智,等.双氢青蒿素抑制胶质瘤干细胞侵袭迁移的机制[J].中国组织工程研究,2016,20(6):765-770.
[9]杨华,谭先杰,郎景和,等.青蒿素衍生物对宫颈癌HeLa细胞体外增殖及凋亡能力的影响及其分子机制研究[J].现代妇产科进展,2017,26(12):881-884.
[10]齐玲,刘玉翠,杨阳,等.双氢青蒿素抑制胶质瘤细胞生长机制[J].中国公共卫生,2015,31(11):1396-1398.