再生水中SS304表面生物膜的活性与结构
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摘要
采用生物化学分析方法、扫描电镜(SEM)和能谱(EDS)研究再生水浓缩倍率对弗氏柠檬酸杆菌(Citrobacter freundii)生物膜脱氢酶活性、胞外聚合物(EPS)组分含量以及SS304表面生物膜形貌和组分的影响。结果表明:弗氏柠檬酸杆菌生物膜脱氢酶的活性随浓缩倍率的提高而增大,3 d时细胞活性最大;EPS中多糖/蛋白质的比值随浓缩倍率的提高而增大,生物膜亲水性增加,黏附性也逐渐增加;随着浓缩倍率的提高,SS304试片表面生物膜厚度也有所增加,生物膜中代谢产物及无机盐组分也有所增加,说明浓缩倍率的提高会加剧SS304表面腐蚀程度。
In this study,we investigated the influence of various concentration ratios of reclaimed water on the dehydrogenase activity of Citrobacter freundii biofilms,quantity of extracellular polymeric substances( EPS),and morphology of biofilms and corroded samples by the microbiological methods,scanning electron microscopy( SEM),and energy dispersion spectrum( EDS). The results showed that the dehydrogenase activity of the biofilms varied regularly according to changes in the concentration ratio,and it peaked on the third day of the analysis. The ratio of polysaccharide and protein content in EPS increased as the concentration ratio increased,so as to the hydrophily of the biofilms and their adhesiveness to impurities. The thickness and fraction of coverage of the biofilms on SS304 increased as the concentration time increased,so as to the metabolites and inorganic salt in the biofilms,which indicated that the increase in concentration time aggravated the corrosion of the SS304 surface.
In this study,we investigated the influence of various concentration ratios of reclaimed water on the dehydrogenase activity of Citrobacter freundii biofilms,quantity of extracellular polymeric substances( EPS),and morphology of biofilms and corroded samples by the microbiological methods,scanning electron microscopy( SEM),and energy dispersion spectrum( EDS). The results showed that the dehydrogenase activity of the biofilms varied regularly according to changes in the concentration ratio,and it peaked on the third day of the analysis. The ratio of polysaccharide and protein content in EPS increased as the concentration ratio increased,so as to the hydrophily of the biofilms and their adhesiveness to impurities. The thickness and fraction of coverage of the biofilms on SS304 increased as the concentration time increased,so as to the metabolites and inorganic salt in the biofilms,which indicated that the increase in concentration time aggravated the corrosion of the SS304 surface.
引文
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[20]MARCUS P.Surface science approach of corrosion phenomena[J].Electrochimica Acta,1998,43(1/2):109-118
[2]焦迪,李进,李娟,等.硫酸盐还原菌在中水中的分离及生长特性研究[J].环境科学与技术,2010,33(10):64-67
[3]许晶晶,李进,李强,等.回用水对冷却水系统316 L不锈钢的腐蚀[J].工业用水与废水,2007,38(4):107-109
[4]李进,许兆义,李久义,等.再生水环境中304不锈钢生物膜腐蚀电化学特征[J].物理化学学报,2010,26(10):2638-2646
[5]LERM S,ALAWI M,MIETHLING-GRAFF R,et al.Influence of microbial processes on the operation of a cold store in a shallow aquifer:Impact on well injectivity and filter lifetime[J].Grundwasser,2011,16(2):93-104
[6]魏仁超,李焰,许凤玲,等.SRB腐蚀与生物矿化作用的研究进展[J].材料开发与应用,2013,28(5):101-108
[7]李付绍.硫酸盐还原菌生物膜下钢铁材料腐蚀行为的研究[D].哈尔滨:哈尔滨工业大学,2009
[8]CHANDEL M,AZMI W.Optimization of process parameters for the production of tyrosine phenol lyase by Citrobacter freundii MTCC 2424[J].Bioresource Technology,2009,100(5):1840-1846
[9]张广文.循环冷却水浓缩倍率计算方法的试验研究[J].热力发电,2008,37(12):90-93
[10]王磊,吴小涛,史志英,等.转化生长因子β1和胰岛素样生长因子1对体外培养人退变髓核细胞生物学活性的影响[J].中国脊柱脊髓杂志,2007,17(3):218-221
[11]祁峰.脱氢酶活性测定水中活体藻含量的研究[D].济南:山东大学,2006
[12]HONG Liu,HERBERT H,FANG P.Extraction of extracellular polymeric substances(EPS)of sludges[J].Journal of Biotechnology,2002,95(3):249-256
[13]齐鲁青,汪晓军,詹德明.优化TTC-脱氢酶还原法测定陶粒负载微生物活性[J].现代化工,2012,32(3):93-96
[14]张静.再生水中弗氏柠檬酸杆菌对铜合金的腐蚀机理研究[D].北京:北京交通大学,2011
[15]廖青,李小明,杨麒,等.好氧颗粒污泥的快速培养以及胞外多聚物对颗粒化的影响研究[J].工业用水与废水,2008,39(4):13-19
[16]王义富.右旋氨基酸对废水处理反应器中微生物聚集体的作用机制[D].济南:山东大学,2014
[17]KYUNG-MAN M,HWANG-RAE C,MYUNG-HOON L,et al.Electrochemical analysis of the microbiologically influenced corrosion of steels by sulfate-reducing bacteria[J].Metals and Materials International,2007,13(3):211-216
[18]SCHMITT J,FLEMMING H.Mikrobielle werkstoffzerstrung-biofilm und biofouling:Die FTIR-spektroskopie zur untersuchung von biofilmen[J].Materials and Corrosion,1994,45(1):58-64
[19]刘彬.海洋好氧生物膜在不锈钢电极表面的附着与腐蚀行为研究[D].北京:中国科学院研究生院,2011
[20]MARCUS P.Surface science approach of corrosion phenomena[J].Electrochimica Acta,1998,43(1/2):109-118