针刺对脑缺血再灌注大鼠肢体运动的影响及对神经细胞再生的保护作用
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摘要
目的:探讨针刺对脑缺血再灌注大鼠肢体运动功能的影响及对神经细胞再生的保护作用。方法:将大鼠随机分为两组,别为假手术组、造模组。造模组大鼠参照Longa的线栓法复制MCAO大鼠模型,造模成功的大鼠再随机分为缺血组和电针组。以针刺百会穴、印堂穴、双侧足三里穴作为干预措施,观察大鼠神经行为学功能(神经功能评分,梯形平衡木检测)、测定BrdU和DCX阳性细胞个数以及免疫荧光检测BrdU和DCX共表达的情况。结果:电针治疗后,神经功能评分、梯形平衡木检测结果均有不同程度下降;BrdU和DCX阳性细胞个数也显著上升,且两者往往共同表达。结论:针刺对脑缺血再灌注大鼠肢体运动恢复有促进作用,对脑神经细胞的再生有保护作用。
Objective:To investigate the effect of acupuncture on limb motor function in rats with cerebral ischemia and reperfusion and its protective effect on nerve cell regeneration. Methods: The rats were randomly divided into sham operation group and model group. The MCAO rats model was established by Longa thread plug method,and the rats were randomly divided into ischemia group and electroacupuncture group. Acupuntrue at Baihui( DU20),Yintang( EX-HN3),Zusanli( ST36) as intervention,we observed neurobehavioral function( nerve function score,trapezoid balance detection),determined Brd U and DCX positive cell number and immunofluorescence was detected Brd U and DCX co expression. Results: After electroacupuncture treatment,the scores of neurological function score and trapezoid balance beam decreased in different degrees. The number of Brd U and DCX positive cells also increased significantly,and both of them often expressed together. Conclusion: Acupuncture can promote the recovery of limb movement in rats with cerebral ischemia and reperfusion,and has a protective effect on the regeneration of brain nerve cells.
Objective:To investigate the effect of acupuncture on limb motor function in rats with cerebral ischemia and reperfusion and its protective effect on nerve cell regeneration. Methods: The rats were randomly divided into sham operation group and model group. The MCAO rats model was established by Longa thread plug method,and the rats were randomly divided into ischemia group and electroacupuncture group. Acupuntrue at Baihui( DU20),Yintang( EX-HN3),Zusanli( ST36) as intervention,we observed neurobehavioral function( nerve function score,trapezoid balance detection),determined Brd U and DCX positive cell number and immunofluorescence was detected Brd U and DCX co expression. Results: After electroacupuncture treatment,the scores of neurological function score and trapezoid balance beam decreased in different degrees. The number of Brd U and DCX positive cells also increased significantly,and both of them often expressed together. Conclusion: Acupuncture can promote the recovery of limb movement in rats with cerebral ischemia and reperfusion,and has a protective effect on the regeneration of brain nerve cells.
引文
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[20] 杨树旭,王义荣,钱聪,等. 老年大鼠脑出血后神经细胞再生的研究[J]. 浙江大学学报(医学版),2008(4):386-392.
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[3] 饶明俐.《中国脑血管病防治指南》摘要 (一)[J].中风与神经疾病杂志,2005, 22(5): 388-393.
[4] 杜元灏,李晶,孙东炜,等.中国现代针灸病谱的研究[J].中国针灸,2007, 27(5):373.
[5] 龚思引,周沐科,郑洪波,等.缺血性脑血管病颈动脉狭窄危险因素分析[J].华西医学,2014,29(2):210-213.
[6] 梁翠兰. 对心脑血管疾病的危险因素及预防方法探讨[J]. 中西医结合心血管病电子杂志,2016,4(34):87-88.
[7] 张艳春,秦江梅,杨晓倩,等.北京市社区慢病患者心脑血管疾病风险预测研究[J].中国全科医学,2017,20(5):590-594.
[8] Shih CC, Yeh CC, Hu CJ, et al. Risk of dementia in patients with non-haemorrhagic stroke receiving acupuncture treatment: a nationwide matched cohort study from Taiwan’s National Health Insurance Research Database[J]. Bmj Open, 2017, 7(6):e013638.
[9] Lu L, Zhang XG, Zhong LLD, et al. Acupuncture for neurogenesis in experimental ischemic stroke: a systematic review and meta-analysis[J]. Scientific Reports, 2016, 6(2016):1-16.
[10] 郑仕平,韩为,储浩然,等.通督调神针灸预处理对脑缺血再灌注大鼠相关微小RNA调控机制的研究[J].针刺研究,2015,40(2):99-103.
[11] Longa EZ,Weinstein PR,Carlson S,et al.Reversible middlecerebral artery occlusion without craniectomy in rats[J].Stroke,1989,20(1) : 84-91.
[12] 郭义.实验动物学[M].北京:中国中医药出版社,2016.
[13] Reynolds BA,Weiss S.Generation of neurons and astrocytes from isolated cells of the adult mammalian central nervous system[J].Science, 1992,255(5052):1707-1710.
[14] Gleeson JG,Lin PT,Flanagan LA,et al. Doublecortin is a microtubule associated protein and is expressed widely by migrating neurons[J]. Neuron,1999,23(2):257-271.
[15] Merz K,Lie DC. Evidence that Doublecortin is dispens-able for the development of adult born neurons in mice[J].PLo S ONE,2013,8(5):e62693.
[16] Jin J,Suzuki H,Hirai S,et al. JNK phosphorylates Ser332 ofdouble-cortin and regulates its function in neurite extension and neuronal migra-tion[J]. Dev Neurobiol,2010,70: 929-942.
[17] Lin P,Alllison DC.Measurement of DNA content and of tritiated thymid-ine and bromodeoxyuridine incorporation by the same cells[J].J Histochem Cyto-chem,1993,41(9):1435-1439.
[18] Nakamura S,Takeda Y,Kanno M,et al.Application of bromodeoxyuridi-ne(Brdu) and anti- Brdu monoclonal antibody for the in vivo analysis of proliferative characteristics of human leukemia cells in bone marrow-s[J].Oncology,1991,48(4):285-295.
[19] 金成日,姜奥,张小卿. 电针对脑缺血再灌注大鼠行为学影响实验研究[J]. 辽宁中医药大学学报,2017,19(8):108-110.
[20] 杨树旭,王义荣,钱聪,等. 老年大鼠脑出血后神经细胞再生的研究[J]. 浙江大学学报(医学版),2008(4):386-392.