南瓜白粉病病原菌及种质资源抗性鉴定
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摘要
为鉴定黑龙江省哈尔滨市香坊区向阳镇南瓜基地白粉病病原菌种类及其生理小种,试验通过形态观察、分子鉴定及甜瓜生理小种鉴别体系抗感反应表现,鉴定该地区白粉病病原菌。结果表明,该白粉病病原菌为单囊壳白粉菌(Podosphaera xanthii),生理小种为2France。试验鉴定64份南瓜种质资源对该生理小种抗感反应,筛选出13份高抗资源用于南瓜抗白粉病育种。研究明确南瓜白粉病病原菌生理小种,筛选得到南瓜高抗种质资源,为南瓜抗白粉病新品种选育和抗病基因克隆奠定基础。
To identify the pathogen and physiological race of powdery mildew in pumpkin base in Xiangyang Township, Xiangfang District, Harbin City, Heilongjiang Province, morphological observation,molecular identification and physiological race identification using melon hosts were done in the study.The results showed that the powdery mildew was race 2 France of Podosphaera xanthi. Then, 64 strains of pumpkin were infected by race 2 France and the reactions of resistance were identified. Finally, 13 strains were selected as resistance materials. In this study, the pathogen and physiological race of local powdery mildew was identified and 13 resistance materials could be used in pumpkin disease resistance breeding and resistance gene cloning.
To identify the pathogen and physiological race of powdery mildew in pumpkin base in Xiangyang Township, Xiangfang District, Harbin City, Heilongjiang Province, morphological observation,molecular identification and physiological race identification using melon hosts were done in the study.The results showed that the powdery mildew was race 2 France of Podosphaera xanthi. Then, 64 strains of pumpkin were infected by race 2 France and the reactions of resistance were identified. Finally, 13 strains were selected as resistance materials. In this study, the pathogen and physiological race of local powdery mildew was identified and 13 resistance materials could be used in pumpkin disease resistance breeding and resistance gene cloning.
引文
[1]李明岩,屈淑平,崔崇士.利用酶活性鉴定南瓜对白粉病菌抗病性的研究[J].东北农业大学学报, 2007, 36(6):737-741.
[2] Vakalounakis D J. Klironomou E, Papadakis A. Species spectrum,host range and distribution of powdery mildews on Cucurbitaceae in Grete[J]. Plant Pathol, 1994, 43:813-818.
[3]张怡,徐克东,陈佩佩,等.河南省周口地区瓜类白粉病的分子鉴定与分析[J].华中师范大学学报:自然科学版, 2013(6):831-835.
[4]肖仲久,李小霞,李斌.贵州省南瓜白粉病病原菌初步鉴定[J].北方园艺, 2012(9):146-148.
[5]李苹芳,朱凌丽,羊杏平,等.江浙沪甜瓜白粉病菌及其生理小种的鉴定[J].中国瓜菜, 2015, 28(6):16-20.
[6]苏瑞.新疆部分地区瓜类白粉病生理小种鉴定及籽用西瓜抗性遗传分析[D].乌鲁木齐:新疆农业大学, 2013.
[7]刘东顺,程鸿,孔维萍,等.甘肃甜瓜主产区白粉病菌生理小种的鉴定[J].中国蔬菜, 2010(6):28-32.
[8]刘秀波,张俊华,崔崇士.南瓜白粉病病原菌鉴定及抗性鉴定方法研究[J].中国瓜菜, 2006(1):10-13.
[9]马鸿艳,魏尊苗,祖元刚,等. 2009-2010年黑龙江省主要瓜类作物白粉病菌生理小种鉴定[J].植物保护学报, 2011(3):287-288.
[10]王佩玲,姚刚,张延波,等.关于草莓白粉菌的分离方法和显微鉴定[J].陕西农业科学, 2017, 63(5):13-16.
[11]张怡,张佩佩,马晓萌,等.河南两地市小麦白粉病菌的分子鉴定和进化分析[J].华北农学报, 2012, 27(1):189-192.
[12]李锡香,朱德蔚.黄瓜种质资源描述规范和数据标准[M].北京:中国农业出版社, 2005.
[13] Ballantyne B. Powdery mildew on Cucurbiraceae:Identity, distribution, host range and sources of resistance[J]. Proc Linnean Soc N S W, 1975, 99:100-120.
[14]臧全宇.网纹甜瓜白粉病的抗性遗传研究[D].杭州:浙江大学,2007.
[15]马原松,裴冬丽,王文静,等.河南省几种白粉菌的ITS序列分析[J].河南农业科学, 2012, 41(9):87-90.
[16] Mohamed Y F, Bardin M, Nicot P C, et al. Causal agents of powdery mildew of Cucurbits in Sudan[J]. Plant Disease, 1995, 79(6):634-636.
[17]张慧君,张佩,吴乔歆,等.淮北地区甜瓜白粉病生理小种的鉴定[J].分子植物育种, 2017(3):1084-1089.
[18]马鸿艳,祖元刚,栾非时.甜瓜种质资源苗期抗白粉病鉴定[J].东北农业大学学报, 2009, 40(12):18-23.
[19]左洪波,张艳菊,秦智伟,等.黄瓜种质资源对霜霉病和白粉病的抗病性鉴定[J].东北农业大学学报, 2010, 41(8):24-27.
[20]刘鸣韬,孙化田,张定法.南瓜优质自交系对白粉病的抗病性评价与应用[J].吉林农业科学, 2011, 36(1):30-32.
[2] Vakalounakis D J. Klironomou E, Papadakis A. Species spectrum,host range and distribution of powdery mildews on Cucurbitaceae in Grete[J]. Plant Pathol, 1994, 43:813-818.
[3]张怡,徐克东,陈佩佩,等.河南省周口地区瓜类白粉病的分子鉴定与分析[J].华中师范大学学报:自然科学版, 2013(6):831-835.
[4]肖仲久,李小霞,李斌.贵州省南瓜白粉病病原菌初步鉴定[J].北方园艺, 2012(9):146-148.
[5]李苹芳,朱凌丽,羊杏平,等.江浙沪甜瓜白粉病菌及其生理小种的鉴定[J].中国瓜菜, 2015, 28(6):16-20.
[6]苏瑞.新疆部分地区瓜类白粉病生理小种鉴定及籽用西瓜抗性遗传分析[D].乌鲁木齐:新疆农业大学, 2013.
[7]刘东顺,程鸿,孔维萍,等.甘肃甜瓜主产区白粉病菌生理小种的鉴定[J].中国蔬菜, 2010(6):28-32.
[8]刘秀波,张俊华,崔崇士.南瓜白粉病病原菌鉴定及抗性鉴定方法研究[J].中国瓜菜, 2006(1):10-13.
[9]马鸿艳,魏尊苗,祖元刚,等. 2009-2010年黑龙江省主要瓜类作物白粉病菌生理小种鉴定[J].植物保护学报, 2011(3):287-288.
[10]王佩玲,姚刚,张延波,等.关于草莓白粉菌的分离方法和显微鉴定[J].陕西农业科学, 2017, 63(5):13-16.
[11]张怡,张佩佩,马晓萌,等.河南两地市小麦白粉病菌的分子鉴定和进化分析[J].华北农学报, 2012, 27(1):189-192.
[12]李锡香,朱德蔚.黄瓜种质资源描述规范和数据标准[M].北京:中国农业出版社, 2005.
[13] Ballantyne B. Powdery mildew on Cucurbiraceae:Identity, distribution, host range and sources of resistance[J]. Proc Linnean Soc N S W, 1975, 99:100-120.
[14]臧全宇.网纹甜瓜白粉病的抗性遗传研究[D].杭州:浙江大学,2007.
[15]马原松,裴冬丽,王文静,等.河南省几种白粉菌的ITS序列分析[J].河南农业科学, 2012, 41(9):87-90.
[16] Mohamed Y F, Bardin M, Nicot P C, et al. Causal agents of powdery mildew of Cucurbits in Sudan[J]. Plant Disease, 1995, 79(6):634-636.
[17]张慧君,张佩,吴乔歆,等.淮北地区甜瓜白粉病生理小种的鉴定[J].分子植物育种, 2017(3):1084-1089.
[18]马鸿艳,祖元刚,栾非时.甜瓜种质资源苗期抗白粉病鉴定[J].东北农业大学学报, 2009, 40(12):18-23.
[19]左洪波,张艳菊,秦智伟,等.黄瓜种质资源对霜霉病和白粉病的抗病性鉴定[J].东北农业大学学报, 2010, 41(8):24-27.
[20]刘鸣韬,孙化田,张定法.南瓜优质自交系对白粉病的抗病性评价与应用[J].吉林农业科学, 2011, 36(1):30-32.