墨鱼缠卵腺糖蛋白的抗氧化及抑菌活性研究
|
摘要
为了探究墨鱼缠卵腺糖蛋白(CGG)的抗氧化活性及抑菌活性,本试验通过测定CGG的超氧阴离子自由基清除率、DPPH自由基清除率、羟基自由基清除能力、小鼠体内过氧化氢酶(CAT)活性及谷胱甘肽过氧化物酶(GSH-PX)活性等研究CGG体内外抗氧化活性,并通过抑菌试验探讨了CGG的抑菌活性。结果表明,随着CGG浓度的增大,超氧阴离子自由基清除率从17.51%增加至55.84%,DPPH自由基清除率从5.7%增加至19.5%,羟基自由基清除能力从10.38%增加至57.94%;与对照组相比,低、中、高剂量组小鼠体内的CAT活性分别提高了21.91%、126.29%、135.63%,GSH-PX活性分别提高了2.04%、24.69%、28.13%。抑菌试验结果表明,当CGG浓度为14 mg·m L-1时,大肠杆菌(Escherichia coli)、金黄色葡萄球菌(Staphylococcus aureus)、假单胞菌(Pseudomonadaceae)的抑菌圈分别为15.7、13.6、12.5mm,MIC分别为1.25、7、7 mg·m L-1,MBC分别为14、28、14 mg·m L-1。综上表明,CGG具有一定的抗氧化和抑菌活性,但抑菌效果较弱。本研究结果为墨鱼缠卵腺的合理利用提供了一定的理论基础,同时对提高水产资源利用率有一定的参考价值。
In order to explore the antioxidant activity and antibacterial activity of cuttlefish gland glycoprotein( CGG),the superoxide anion radical scavenging rate,DPPH free radical scavenging rate,hydroxyl radical scavenging ability were measured to study the anti-oxidation actirities of CGG in ritro and in vivo. Meanwhile,the antibacterial activity of CGG was studied by antibacterial experiments. It was found that the superoxide anion radical scavenging rate,the DPPH free radical scavenging rate and the hydroxyl radical scavenging ability increased were correspondingly increased from 17.51%,5.7% and 10.38% to 55.84%,19.5%,57.94% with the increased of CGG concentration,respectively. Compared with the control groups the catalase( CAT) activity in mice with low,medium and high dose were increased by 21.91%,126.29%,135.63%,respectively. Furthermore,the glutathione peroxidase( GSH-PX) activity in the low,medium and high dose groups was increased by 2.04%,24.69%,and 28.13%,respectively. The antibacterial experiments showed that when the CGG concentration was 14 mg·m L-1,the inhibition zones of Escherichia coli,Staphylococcus aureus,and Pseudomonadaceae were 15.7,13.6 and 12.5 mm,MIC were 1.25,7,and 7 mg·m L-1,and the MBC were 14,28,and14 mg·m L-1,respectively. The results indicated that the cuttlefish gland glycoprotein squid had certain but relatively weak antioxidant and antibacterial activities. The results can provide certain theoretical basis for the rational utilization of the squid entangled gland,and reference for improving the utilization rate of aquatic resources.
In order to explore the antioxidant activity and antibacterial activity of cuttlefish gland glycoprotein( CGG),the superoxide anion radical scavenging rate,DPPH free radical scavenging rate,hydroxyl radical scavenging ability were measured to study the anti-oxidation actirities of CGG in ritro and in vivo. Meanwhile,the antibacterial activity of CGG was studied by antibacterial experiments. It was found that the superoxide anion radical scavenging rate,the DPPH free radical scavenging rate and the hydroxyl radical scavenging ability increased were correspondingly increased from 17.51%,5.7% and 10.38% to 55.84%,19.5%,57.94% with the increased of CGG concentration,respectively. Compared with the control groups the catalase( CAT) activity in mice with low,medium and high dose were increased by 21.91%,126.29%,135.63%,respectively. Furthermore,the glutathione peroxidase( GSH-PX) activity in the low,medium and high dose groups was increased by 2.04%,24.69%,and 28.13%,respectively. The antibacterial experiments showed that when the CGG concentration was 14 mg·m L-1,the inhibition zones of Escherichia coli,Staphylococcus aureus,and Pseudomonadaceae were 15.7,13.6 and 12.5 mm,MIC were 1.25,7,and 7 mg·m L-1,and the MBC were 14,28,and14 mg·m L-1,respectively. The results indicated that the cuttlefish gland glycoprotein squid had certain but relatively weak antioxidant and antibacterial activities. The results can provide certain theoretical basis for the rational utilization of the squid entangled gland,and reference for improving the utilization rate of aquatic resources.
引文
[1]蒋霞敏,彭瑞冰,罗江,高秀芝,姜小敏.野生拟目乌贼不同组织营养成分分析及评价[J].动物营养学报,2012,24(12):2393-2401
[2]戴宏杰,孙玉林,冯梓欣,文菁,陈道海.雌性虎斑乌贼缠卵腺营养成分分析与评价[J].食品科学,2016,37(14):97-103
[3]曾婷婷,徐明生,蒋艳.超声波法提取河蚬糖蛋白[J].江西农业大学学报,2008,30(1):144-148
[4]秦玉青.扇贝生殖腺糖蛋白的提取与组分分析[J].渔业现代化,2001,6(1):39-40
[5] Lei X L,Fan X P,Zhao S J,Chen M,He Q L. Study on extracting and fractionating of glycoprotein of dried octopus[J]. Journal of Shaanxi University of Sclence&Technolog,2006,24(4):45-49
[6]包郁眀.皱纹盘鲍糖蛋白分离纯化及其免疫活性的研究[D].青岛:中国海洋大学,2012
[7] Shigeru K,Yoko S,Haruo M,Noboru K,Yutaka H. Occurrence of a mucin-type glycoprotein in nidamental gland mucosubstance from the squid Illexargentinus[J]. Fisheries Science,1994,60(2):193-197
[8]王倩,刘淑集,林彩平,吴成业.响应面法优化鱿鱼缠卵腺糖蛋白提取工艺研究[J].食品工业科技,2014,35(3):261-265
[9]刘淑集,王倩,王茵,刘智禹,苏永昌,吴成业.鱿鱼缠卵腺糖蛋白MGS降血脂及非特异性免疫功能研究[J].福建水产,2014,36(6):436-441
[10]王春琳,王津伟,余红卫,宋微微,宋超霞.曼氏无针乌贼(Sepiella maindroni)副缠卵腺的组织学及超微结构[J].海洋与湖沼,2010,41(3):391-395
[11]薛张芝,张洪超,丁源,徐晓蓉,陆语城,李和生,王鸿飞,许凤.墨鱼缠卵腺糖蛋白提取工艺及纯化[J].食品工业科技,40(6):167-172,178
[12]方旭波,陈小娥,余辉,宋茹.鱿鱼硫酸软骨素糖蛋白的分离纯化和鉴定[J].中国食品学报,2009,9(4):103-109
[13]毛方华,王鸿飞,林燕,刘飞.油茶籽毛油中多酚类物质对自由基的清除作用[J].中国粮油学报,2010,25(1):64-68
[14]吕禹泽,宋钰,吴国宏,熊何健.葡萄多酚的抗氧化活性[J].食品科学,2006,27(12):213-216
[15] Tang H S,Chen P Y,Ding Q,Liu L Y,Qi H,Li L. Extraction of soluble dietary fiber from pomelo peel by ultrasonic assisted enzymatic method and its antioxidant activity[J]. Storage&Process,2016,16(6):103-106
[16]颜军,苟小军,邹全付,纪小明,崔秀英,兰海蓉,李雪.分光光度法测定Fenton反应产生的羟基自由基[J].成都大学学报(自然科学版),2009,28(2):91-93,103
[17]赵谋明,刘洋,张佳男,吴长平,苏国万.木糖-草鱼肽美拉德反应产物的抗氧化性[J].农业工程学报,2014,30(9):279-286
[18]郑春红,徐昌平,李晓梅,黄涵年,郭江峰.耐辐射动球菌类胡萝卜素提取物的抗氧化活性研究[J].核农学报,2017,31(2):280-287
[19] Choi W S,Chang S H,Kim J E,Lee S E. Hypolipidemic effects of scoparone and its coumarin analogues in hyperlipidemia rats induced by high fat diet[J]. Journal of the Korean Society for Applied Biological Chemistry,2013,56(6):647-653
[20]徐超,王鸿飞,邵兴锋,俞雯雯.香榧子油抗氧化活性及降血脂功能研究[J].中国粮油学报,2012,27(8):43-47
[21]王鸿飞,刘飞,徐超,林燕,李和生,邵兴锋.费菜总黄酮及其不同极性提取物抑菌活性研究[J].中国食品学报,2013,13(5):124-128
[22]魏好程,邵杰,何传波,李漫,熊何健.鲍内脏多糖体内抗氧化及增强小鼠免疫活性[J].食品科学,2018,39(9):140-144
[23]陈双,张娜,牛付阁.玉竹糖蛋白体内抗氧化作用研究[J].食品与药品,2012,14(7):250-253
[24]王倩.太平洋褶柔鱼缠卵腺糖蛋白的分离提取及活性分析[D].福州:福建农林大学,2013
[25]李雨哲,任娇艳,赵谋明.马氏珍珠贝肉糖蛋白的提取工艺优化及其抗氧化活性的研究[J].食品工业科技,2011,31(4):243-245
[26]夏秀芳,王博,郑幸子,邓思杨,潘男,王浩.大豆糖蛋白的抗氧化特性[J].食品科学,2018,39(7):91-97
[27]周茜,吴梦颖,王唯霖,杨光,郝红伟,赵文,王颉.多糖型扇贝沙司对小鼠免疫功能和抗氧化活性的影响[J].中国食品学报,2018,18(2):30-36
[28]牛付阁,王纪平,王芳,戴成国,段玉峰.覆盆子糖蛋白粗提物体内抗氧化作用研究[J].食品工业科技,2010,31(12):134-136
[29]付丹.卵粘蛋白抑菌活性及其机制的研究[D].武汉:华中农业大学,2015
[30]师晓栋.玫瑰尤须鳃卵黄蛋自原和卵黄脂磷蛋自的纯化、鉴定、免疫分析和功能研究[D].青岛:中国海洋大学,2004
[2]戴宏杰,孙玉林,冯梓欣,文菁,陈道海.雌性虎斑乌贼缠卵腺营养成分分析与评价[J].食品科学,2016,37(14):97-103
[3]曾婷婷,徐明生,蒋艳.超声波法提取河蚬糖蛋白[J].江西农业大学学报,2008,30(1):144-148
[4]秦玉青.扇贝生殖腺糖蛋白的提取与组分分析[J].渔业现代化,2001,6(1):39-40
[5] Lei X L,Fan X P,Zhao S J,Chen M,He Q L. Study on extracting and fractionating of glycoprotein of dried octopus[J]. Journal of Shaanxi University of Sclence&Technolog,2006,24(4):45-49
[6]包郁眀.皱纹盘鲍糖蛋白分离纯化及其免疫活性的研究[D].青岛:中国海洋大学,2012
[7] Shigeru K,Yoko S,Haruo M,Noboru K,Yutaka H. Occurrence of a mucin-type glycoprotein in nidamental gland mucosubstance from the squid Illexargentinus[J]. Fisheries Science,1994,60(2):193-197
[8]王倩,刘淑集,林彩平,吴成业.响应面法优化鱿鱼缠卵腺糖蛋白提取工艺研究[J].食品工业科技,2014,35(3):261-265
[9]刘淑集,王倩,王茵,刘智禹,苏永昌,吴成业.鱿鱼缠卵腺糖蛋白MGS降血脂及非特异性免疫功能研究[J].福建水产,2014,36(6):436-441
[10]王春琳,王津伟,余红卫,宋微微,宋超霞.曼氏无针乌贼(Sepiella maindroni)副缠卵腺的组织学及超微结构[J].海洋与湖沼,2010,41(3):391-395
[11]薛张芝,张洪超,丁源,徐晓蓉,陆语城,李和生,王鸿飞,许凤.墨鱼缠卵腺糖蛋白提取工艺及纯化[J].食品工业科技,40(6):167-172,178
[12]方旭波,陈小娥,余辉,宋茹.鱿鱼硫酸软骨素糖蛋白的分离纯化和鉴定[J].中国食品学报,2009,9(4):103-109
[13]毛方华,王鸿飞,林燕,刘飞.油茶籽毛油中多酚类物质对自由基的清除作用[J].中国粮油学报,2010,25(1):64-68
[14]吕禹泽,宋钰,吴国宏,熊何健.葡萄多酚的抗氧化活性[J].食品科学,2006,27(12):213-216
[15] Tang H S,Chen P Y,Ding Q,Liu L Y,Qi H,Li L. Extraction of soluble dietary fiber from pomelo peel by ultrasonic assisted enzymatic method and its antioxidant activity[J]. Storage&Process,2016,16(6):103-106
[16]颜军,苟小军,邹全付,纪小明,崔秀英,兰海蓉,李雪.分光光度法测定Fenton反应产生的羟基自由基[J].成都大学学报(自然科学版),2009,28(2):91-93,103
[17]赵谋明,刘洋,张佳男,吴长平,苏国万.木糖-草鱼肽美拉德反应产物的抗氧化性[J].农业工程学报,2014,30(9):279-286
[18]郑春红,徐昌平,李晓梅,黄涵年,郭江峰.耐辐射动球菌类胡萝卜素提取物的抗氧化活性研究[J].核农学报,2017,31(2):280-287
[19] Choi W S,Chang S H,Kim J E,Lee S E. Hypolipidemic effects of scoparone and its coumarin analogues in hyperlipidemia rats induced by high fat diet[J]. Journal of the Korean Society for Applied Biological Chemistry,2013,56(6):647-653
[20]徐超,王鸿飞,邵兴锋,俞雯雯.香榧子油抗氧化活性及降血脂功能研究[J].中国粮油学报,2012,27(8):43-47
[21]王鸿飞,刘飞,徐超,林燕,李和生,邵兴锋.费菜总黄酮及其不同极性提取物抑菌活性研究[J].中国食品学报,2013,13(5):124-128
[22]魏好程,邵杰,何传波,李漫,熊何健.鲍内脏多糖体内抗氧化及增强小鼠免疫活性[J].食品科学,2018,39(9):140-144
[23]陈双,张娜,牛付阁.玉竹糖蛋白体内抗氧化作用研究[J].食品与药品,2012,14(7):250-253
[24]王倩.太平洋褶柔鱼缠卵腺糖蛋白的分离提取及活性分析[D].福州:福建农林大学,2013
[25]李雨哲,任娇艳,赵谋明.马氏珍珠贝肉糖蛋白的提取工艺优化及其抗氧化活性的研究[J].食品工业科技,2011,31(4):243-245
[26]夏秀芳,王博,郑幸子,邓思杨,潘男,王浩.大豆糖蛋白的抗氧化特性[J].食品科学,2018,39(7):91-97
[27]周茜,吴梦颖,王唯霖,杨光,郝红伟,赵文,王颉.多糖型扇贝沙司对小鼠免疫功能和抗氧化活性的影响[J].中国食品学报,2018,18(2):30-36
[28]牛付阁,王纪平,王芳,戴成国,段玉峰.覆盆子糖蛋白粗提物体内抗氧化作用研究[J].食品工业科技,2010,31(12):134-136
[29]付丹.卵粘蛋白抑菌活性及其机制的研究[D].武汉:华中农业大学,2015
[30]师晓栋.玫瑰尤须鳃卵黄蛋自原和卵黄脂磷蛋自的纯化、鉴定、免疫分析和功能研究[D].青岛:中国海洋大学,2004