不同干燥方式对天麻多指标成分含量的影响
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摘要
采用晒干、不同温度烘干、微波干燥和冻干方式干燥天麻,利用HPLC方法测定多指标成分的含量。建立天麻活性成分多指标同时检测的分析方法,考察不同干燥方式对天麻外观性状及多指标成分含量的影响。以天麻素、对羟基苯甲醇、巴利森苷A作为主要指标,只考虑温度因素时,80℃烘干效果最好;以腺苷含量作为主要指标,晒干处理方式下腺苷含量最高;以对羟基苯甲醛作为主要指标,晒干跟微波干燥的样品含量差距不大,冻干方式含量最低。天麻多指标成分含量同时检测方法的建立,可根据实际需要选择不同的采后干燥加工方式,综合考虑外观性状及指标成分含量的变化,天麻60℃烘干效果较好。
The Gastrodiae Rhizoma was dried by sun-drying,drying at different temperatures,microwave drying and freeze-drying,and the content of the multi-indicative component was determined by HPLC. An analytical method for simultaneous detection of multi-indicator components of Gastrodiae Rhizoma was established,and the effects of different drying methods on appearance and multi-indicator content of Gastrodiae Rhizoma were investigated. Gastrodin,p-Hydroxybenzyl alcohol,and Barryntoside A as the main index,and just considering on the temperature factor,80 ℃ was the best drying method; Adenosine content as the main indicator, the sun drying was the best way; Using hydroxybenzaldehyde as the main index,there is little difference in sample content between sun-drying and microwavedrying,and the content of freeze-drying method is the lowest. Through the establishment of simultaneous detectionmethods for multi-index components of Gastrodiae Rhizoma,different post-harvest drying methods can be selected according to actual needs. By comparing the appearance and the change of the index component content,the drying effect at 60 ℃ is better for Gastrodiae Rhizoma.
The Gastrodiae Rhizoma was dried by sun-drying,drying at different temperatures,microwave drying and freeze-drying,and the content of the multi-indicative component was determined by HPLC. An analytical method for simultaneous detection of multi-indicator components of Gastrodiae Rhizoma was established,and the effects of different drying methods on appearance and multi-indicator content of Gastrodiae Rhizoma were investigated. Gastrodin,p-Hydroxybenzyl alcohol,and Barryntoside A as the main index,and just considering on the temperature factor,80 ℃ was the best drying method; Adenosine content as the main indicator, the sun drying was the best way; Using hydroxybenzaldehyde as the main index,there is little difference in sample content between sun-drying and microwavedrying,and the content of freeze-drying method is the lowest. Through the establishment of simultaneous detectionmethods for multi-index components of Gastrodiae Rhizoma,different post-harvest drying methods can be selected according to actual needs. By comparing the appearance and the change of the index component content,the drying effect at 60 ℃ is better for Gastrodiae Rhizoma.
引文
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[20]JI W H,CHEN L X,MA X L,et al. Molecularly imprinted polymers with novel functional monomer for selective solid-phase extraction of gastrodin from the aqueous extract of Gastrodia elata.[J]. Journal of Chromatography A,2014,1342:1-7. DOI:10. 1016/j. chroma. 2014. 03. 046.
[21]葛进,刘大会,崔秀明,等.昭通产乌天麻的变温干燥工艺研究[J].中草药,2015,46(24):3675-3681.
[22]吕紫璇,车早红,唐瑜,等.蒸制对乌天麻中天麻素和对羟基苯甲醇的含量的影响[J].中医临床研究,2017,9(1):44-45.
[2]王伟,文欢,张大燕,等.不同类型天麻的品质比较[J].食品工业,2018,39(1):305-308.
[3]杨超,吕紫媛,伍瑞云.天麻的化学成分与药理机制研究进展[J].中国现代医生,2012,50(17):27-28.
[4]郭驹,马晓俞,于朝霞,等.养血清脑颗粒、天麻素联合尼莫地平治疗偏头痛60例[J].中国药业,2014,23(6):70-71.
[5]SUN W,MIAO B,WANG X C,et al. Gastrodin inhibits allodynia and hyperalgesia in painful diabetic neuropathy rats by decreasing excitability of nociceptive primary sensory neurons[J]. Plo S One,2012,7(6):e39647. DOI:10. 1371/journal. pone.0039647.
[6]洪全,陈淼,李雪萍.天麻药理研究进展[J].中国实用医药,2010,5(11):249-250.
[7]王亚威,李志峰,何明珍,等.天麻化学成分研究[J].中草药,2013,44(21):2974-2976.
[8]刘毅,张丽艳,谢宇,等.天麻最佳炮制工艺的综合评分法研究[J].时珍国医国药,2009,20(2):378-379.
[9]田治蛟,王家金,刘金美,等.不同干燥方法和蒸制时间对昭通天麻药材质量的影响[J].西南农业学报,2016,29(7):1701-1706.
[10]葛进,刘大会,鲁惠珍,等.蒸制断生后真空冷冻干燥对天麻质量的影响[J].中国医院药学杂志,2016,36(3):180-186.
[11]祝洪艳,蒋然,何忠梅,等.不同乌天麻炮制品中天麻素、天麻苷元和天麻多糖的含量分析[J].中国药学杂志,2017,52(23):2062-2065.
[12]王信,王徽,杨飞,等.天麻中6种天麻素类成分一测多评分析法的建立[J].中草药,2018,49(3):694-699.
[13]季德,宁子琬,张雪荣,等.不同干燥加工方法对天麻药材质量的影响[J].中国中药杂志,2016,41(14):2587-2590.
[14]刘彦铎,王昌利,唐斌,等.不同干燥方法对天麻中天麻素含量的影响[J].现代中医药,2013,33(3):108-109.
[15]周劲松,张洪坤,黄玉瑶,等.天麻不同软化方法的比较及天麻片炮制工艺优化研究[J].时珍国医国药,2016,27(3):622-624.
[16]黄先敏,祁岑,朱玉勇,等.鲜天麻及不同加工天麻中天麻苷元和天麻素含量的测定[J].昭通学院学报,2017,39(5):42-47.
[17]WU J Y,WU B C,TANG C L,et al. Analytical techniques and pharmacokinetics of Gastrodia elata Blume and its constituents.[J]. Molecules,2017,22(7):E1137. DOI:10. 3390/molecules22071137.
[18]LAI C J S,YUAN Y,LIU D H,et al. Untargeted metabolite analysis-based UHPLC-Q-TOF-MS reveals significant enrichment of p-hydroxybenzyl dimers of citric acids in fresh beige-scape Gastrodia elata(Wutianma)[J]. Journal of Pharmaceutical&Biomedical Analysis,2017,140:287-294. DOI:10. 1016/j. jpba. 2017. 03. 055.
[19]尹珉,毛克臣,陈志峰,等.不同炮制方法对天麻中天麻素含量的影响[J].中国中医药信息杂志,2010,17(7):49-50.
[20]JI W H,CHEN L X,MA X L,et al. Molecularly imprinted polymers with novel functional monomer for selective solid-phase extraction of gastrodin from the aqueous extract of Gastrodia elata.[J]. Journal of Chromatography A,2014,1342:1-7. DOI:10. 1016/j. chroma. 2014. 03. 046.
[21]葛进,刘大会,崔秀明,等.昭通产乌天麻的变温干燥工艺研究[J].中草药,2015,46(24):3675-3681.
[22]吕紫璇,车早红,唐瑜,等.蒸制对乌天麻中天麻素和对羟基苯甲醇的含量的影响[J].中医临床研究,2017,9(1):44-45.