基于SSR标记的不同优势等级云南松遗传多样性分析
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摘要
基于SSR分子标记技术,按树高和胸径(地径)因子将样地内的植株分为优势木、中等木和劣势木3类,对不同生长优势等级云南松林木的遗传多样性变异特征进行了研究。结果表明:在林冠层,优势木的有效等位基因数、Shannon’s信息指数、观测杂合度和期望杂合度分别为2.083、0.762、0.290和0.423,略高于中等木和劣势木;在更新层,有效等位基因数、Shannon’s信息指数、观测杂合度和期望杂合度分别为2.063、0.774、0.272和0.410,除观测杂合度外,其余3个指标也均表现为优势木略高于中等木和劣势木。从差异显著性检验来看,不同生长优势等级云南松林木间的遗传多样性差异不显著,即林木生长分化对遗传多样性的影响不明显。
The trees were divided into the dominant trees,the intermediate trees and the suppressed trees according to the height and diameter(ground diameter) parameters.The variation of genetic diversity characteristics in different dominance hierarchies of Pinus yunnanensis Franch.population was studied by SSR molecular markers.In forest canopy layer,the mean number of effective alleles,Shannon's information index,observed heterozygosity and expected heterozygosity of dominant trees were 2.083,0.762,0.290 and 0.423,respectively,for the dominant trees,which slightly exceeded the intermediate and suppressed trees.In regeneration layer mean number of effective alleles,Shannon's information index,observed heterozygosity and expected heterozygosity were 2.063,0.774,0.272 and 0.410,respectively,for the dominant tree,which slightly exceeded the intermediate and suppressed trees except for observed heterozygosity.There were no significant differences among the different dominance hierarchy trees for the genetic diversity by the difference significance test of statistics method.
The trees were divided into the dominant trees,the intermediate trees and the suppressed trees according to the height and diameter(ground diameter) parameters.The variation of genetic diversity characteristics in different dominance hierarchies of Pinus yunnanensis Franch.population was studied by SSR molecular markers.In forest canopy layer,the mean number of effective alleles,Shannon's information index,observed heterozygosity and expected heterozygosity of dominant trees were 2.083,0.762,0.290 and 0.423,respectively,for the dominant trees,which slightly exceeded the intermediate and suppressed trees.In regeneration layer mean number of effective alleles,Shannon's information index,observed heterozygosity and expected heterozygosity were 2.063,0.774,0.272 and 0.410,respectively,for the dominant tree,which slightly exceeded the intermediate and suppressed trees except for observed heterozygosity.There were no significant differences among the different dominance hierarchy trees for the genetic diversity by the difference significance test of statistics method.
引文
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20.蔡年辉,李根前,束传林,等.云南松天然林区植物群落结构的空间动态研究[J].西北植物学报,2006,26(10):2119-2124.Cai N H,Li G Q,Su C L,et al.Spatial dynamics of plant community structures in natural Pinus yunnanensis forest region[J].Acta Botanica Boreali-Occidentalia Sinica,2006,26(10):2119-2124.
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2.李巍,王传宽,张全智.林木分化对兴安落叶松异速生长方程和生物量分配的影响[J].生态学报,2015,35(6):1679-1687.Li W,Wang C K,Zhang Q Z.Differentiation of stand individuals impacts allometry and biomass allocation of Larix gmelinii trees[J].Acta Ecologica Sinica,2015,35(6):1679-1687.
3.李莲芳,韩明跃,郑畹,等.云南松低质低效林的成因及其分类[J].西部林业科学,2009,38(4):94-99.Li L F,Han M Y,Zheng W,et al.The causes of formation of low quality forest of Pinus yunnanensis and their classification[J].Journal of West China Forestry Science,2009,38(4):94-99.
4.李振华,王彦辉,于澎涛,等.华北落叶松液流速率的优势度差异及其对林分蒸腾估计的影响[J].林业科学研究,2015,28(1):8-16.Li Z H,Wang Y H,Yu P T,et al.Variation of sap flow density of Larix principis-rupprechtii with dominances and its impact on stand transpiration estimation[J].Forest Research,2015,28(1):8-16.
5.陈波,鲁绍伟,李少宁.不同优势等级杨树人工林吸收臭氧特征[J].北京林业大学学报,2015,37(7):29-36.Chen B,Lu S W,Li S N.Ozone uptake characteristics in different dominance hierarchies of poplar plantation[J].Journal of Beijing Forestry University,2015,37(7):29-36.
6.刘家霖,满秀玲,胡悦.兴安落叶松天然林不同分化等级林木树干液流对综合环境因子的响应[J].林业科学研究,2016,29(5):726-734.Liu J L,Man X L,Hu Y.Response of tree sap flow of Larix gmelinii with various differentiation classes to multiple environmental factors[J].Forest Research,2016,29(5):726-734.
7.万艳芳,于澎涛,刘贤德,等.祁连山青海云杉树干液流密度的优势度差异[J].生态学报,2017,37(9):3106-3114.Wan Y F,Yu P T,Liu X D,et al.Variation in sap flow density among levels of tree dominance in Picea crassifolia in the Qilian Mountains[J].Acta Ecologica Sinica,2017,37(9):3106-3114.
8.李德志,臧润国.森林冠层结构与功能及其时空变化研究进展[J].世界林业研究,2004,17(3):12-16.Li D Z,Zang R G.The research advances on the structure and function of forest canopy,as well as their temporal and spatial changes[J].World Forestry Research,2004,17(3):12-16.
9.许格希,史作民,唐敬超,等.海南尖峰岭热带山地雨林林冠层树种功能多样性特征[J].应用生态学报,2016,27(11):3444-3454.Xu G X,Shi Z M,Tang J C,et al.Functional diversity characteristics of canopy tree species of Jianfengling tropical montane rainforest on Hainan Island,China[J].Chinese Journal of Applied Ecology,2016,27(11):3444-3454.
10.玉宝,张秋良,王立明.中幼龄兴安落叶松过伐林垂直结构综合特征[J].林业科学,2015,51(1):132-139.Yu B,Zhang Q L,Wang L M.Comprehensive characteristics of the vertical structure of middle young over cutting forest of Larix gmelinii[J].Scientia Silvae Sinicae,2015,51(1):132-139.
11.康冰,王得祥,崔宏安,等.秦岭山地油松群落更新特征及影响因子[J].应用生态学报,2011,22(7):1659-1667.Kang B,Wang D X,Cui H A,et al.Regeneration characteristics and related affecting factors of Pinus tabulaeformis secondary forests in Qinling Mountains[J].Chinese Journal of Applied Ecology,2011,22(7):1659-1667.
12.董明亮,高嘉玥,孙文婷,等.北京市华北落叶松优树群体遗传多样性分析[J].植物遗传资源学报,2016,17(4):616-624.Dong M L,Gao J Y,Sun W T,et al.Genetic diversity and population structure of elite Larix principis-rupprechtii Mayr in Beijing[J].Journal of Plant Genetic Resources,2016,17(4):616-624.
13.许玉兰,蔡年辉,徐杨,等.云南松主分布区天然群体的遗传多样性及保护单元的构建[J].林业科学研究,2015,28(6):883-891.Xu Y L,Cai N H,Xu Y,et al.Genetic variation and conservation units analysis in Pinus yunnanensis natural populations[J].Forest Research,2015,28(6):883-891.
14.许玉兰,蔡年辉,陈诗,等.云南松天然群体遗传变异与生态因子的相关性[J].生态学杂志,2016,35(7):1767-1775.Xu Y L,Cai N H,Chen S,et al.Relationships between the genetic diversity of Pinus yunnanensis Franch.natural populations and ecological factors[J].Chinese Journal of E-cology,2016,35(7):1767-1775.
15.Xu Y L,Cai N H,Woeste K,et al.Genetic diversity and population structure of Pinus yunnanensis by simple sequence repeat markers[J].Forest Science,2016,62(1):38-47.
16.胡仲义,付涛,何月秋,等.铁皮石斛EST-SSR引物的评价、甄选与应用研究[J].植物研究,2017,37(1):78-87.Hu Z Y,Fu T,He Y Q,et al.Evaluation,selection and application of Dendrobium officinale EST-SSR primers[J].Bulletin of Botanical Research,2017,37(1):78-87.
17.包文泉,乌云塔娜,杜红岩,等.基于SSR标记的西藏光核桃群体遗传多样性和遗传结构分析[J].林业科学,2018,54(2):30-41.Bao W Q,Wuyun T N,Du H Y,et al.Genetic diversity and population structure of Amygdalus mira in the Tibet Plateau in China based on SSR markers[J].Scientia Silvae Sinicae,2018,54(2):30-41.
18.王久利,陈世龙,邢睿,等.椭圆叶花锚简化基因组的SSR信息分析及SSR引物开发[J].植物研究,2018,38(2):292-297.Wang J L,Chen S L,Xing R,et al.Simplified genome SSRinformation and development of SSR primers of Halenia ellipitica(Gentianaceae)[J].Bulletin of Botanical Research,2018,38(2):292-297.
19.苗作云,杨赟,刘攀峰,等.基于转录组数据的杜仲红叶性状SSR分子标记分析[J].植物研究,2017,37(6):897-906.Miao Z Y,Yang Y,Liu P F,et al.Analysis of red leaf color SSR molecular markers by transcriptome sequencing of Eucommia ulmoides[J].Bulletin of Botanical Research,2017,37(6):897-906.
20.蔡年辉,李根前,束传林,等.云南松天然林区植物群落结构的空间动态研究[J].西北植物学报,2006,26(10):2119-2124.Cai N H,Li G Q,Su C L,et al.Spatial dynamics of plant community structures in natural Pinus yunnanensis forest region[J].Acta Botanica Boreali-Occidentalia Sinica,2006,26(10):2119-2124.
21.梅婷婷,王传宽,赵平,等.木荷树干液流的密度特征[J].林业科学,2010,46(1):40-47.Mei T T,Wang C K,Zhao P,et al.Dynamics of trunk sap flux density of Schima superba[J].Scientia Silvae Sinicae,2010,46(1):40-47.
22.Doyle J J,Doyle J L.Isolation of plant DNA from fresh tissue[J].Focus,1990,12(1):13-15.
23.白青松,张瑞丽,许玉兰,等.云南松成熟针叶DNA提取方法的比较研究[J].广东农业科学,2013,40(4):121-123.Bai Q S,Zhang R L,Xu Y L,et al.Comparative study on total DNA extraction methods of mature needles of Pinus yunnanensis[J].Guangdong Agricultural Sciences,2013,40(4):121-123.
24.Elsik C G,Minihan V T,Hall S E,et al.Low-copy microsatellite markers for Pinus taeda L.[J].Genome,2000,43(3):550-555.
25.Kutil B L,Williams C G.Triplet-repeat microsatellites shared among hard and soft pines[J].The Journal of Heredity,2001,92(4):327-332.
26.Devey M E,Bell J C,Uren T L,et al.A set of microsatellite markers for fingerprinting and breeding applications in Pinus radiata[J].Genome,2002,45(5):984-989.
27.Xu Y L,Zhang R L,Tian B,et al.Development of novel microsatellite markers for Pinus yunnanensis and their cross amplification in congeneric species[J].Conservation Genetics Resources,2013,5(4):1113-1114.
28.Cai N H,Xu Y L,Xu Y,et al.Development and characterization of novel microsatellite loci for Pinus yunnanensis from transcriptome using next-generation sequencing[C].//Basiita R K,Bruggemann J H,Cai N,et al.Microsatellite records for volume 7,issue 4.Conservation Genetics Resources,2015,7:917-944.
29.张瑞丽,许玉兰,王大玮,等.云南松SSR-PCR反应体系的建立与优化[J].生物技术通报,2012(4):93-97.Zhang R L,Xu Y L,Wang D W,et al.Establishment and optimization of the SSR reaction system for Pinus yunnanensis using orthogonal design[J].Biotechnology Bulletin,2012(4):93-97.
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