响应面法优化超声辅助提取龙眼叶总黄酮的工艺研究
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摘要
文章以龙眼叶为原料,以乙醇溶液为溶剂,在单因素的基础上,采用响应面Box-Benhnken试验设计对超声辅助提取龙眼叶总黄酮的工艺进行优化,确定最佳工艺条件为:乙醇浓度90%,液料比48∶1,提取温度61℃,提取时间132min。龙眼叶总黄酮得率为181.83mg/g,与预测值177.32mg/g相接近,因此,文章所采用的响应面法优化超声辅助提取龙眼叶总黄酮的工艺稳定可靠,该工艺可为龙眼叶的综合利用及龙眼叶总黄酮的产业化应用提供一定的理论支撑。
In this paper,on the basis of single factor,the response surface method is used to optimize the ultrasonic-assisted extraction of total flavonoids from longan leaves using ethanol solution as the solvent.The optimal conditions of ultrasonic-assisted extraction are concluded as follows:ethanol concentration is 90%,ratio of liquid to solid is 48∶1,ultrasonic temperature is 61℃,ultrasonic time is 132 min.The actual detection value of total flavonoids yield is 181.83 mg/g,which is close to the predicted value of 177.32 mg/g.Therefore,optimizing the ultrasonic-assisted extraction of total flavonoids from longan leaves using the response surface method is stable and reliable,which provides a theoretical support for the comprehensive utilization of longan leaves and the industrial application of total flavonoids from longan leaves.
In this paper,on the basis of single factor,the response surface method is used to optimize the ultrasonic-assisted extraction of total flavonoids from longan leaves using ethanol solution as the solvent.The optimal conditions of ultrasonic-assisted extraction are concluded as follows:ethanol concentration is 90%,ratio of liquid to solid is 48∶1,ultrasonic temperature is 61℃,ultrasonic time is 132 min.The actual detection value of total flavonoids yield is 181.83 mg/g,which is close to the predicted value of 177.32 mg/g.Therefore,optimizing the ultrasonic-assisted extraction of total flavonoids from longan leaves using the response surface method is stable and reliable,which provides a theoretical support for the comprehensive utilization of longan leaves and the industrial application of total flavonoids from longan leaves.
引文
[1]梁洁,柳贤福,李耀华,等.龙眼叶药材HPLC指纹图谱研究[J].中国实验方剂学杂志,2013,19(18):120-122.
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[5]Liu Y.Antioxidant activity of longan(Dimocarpus longan)barks and leaves[J].African Journal of Biotechnology,2012,11(27):7038-7045.
[6]梁洁,余靓,柳贤福,等.龙眼叶不同提取物降血糖的实验研究[J].时珍国医国药,2013,24(8):2057-2058.
[7]梁洁,滕建北,柳贤福,等.龙眼叶化学成分预试研究[J].中国民族民间医药,2010,19(4):142-143.
[8]陈秋荣.黄酮类化合物药理作用的分析[J].中国实用医药,2012,7(21):254-255.
[9]刘星雨,周敏,孙体健.天然黄酮类化合物的药理活性及分离提取[J].中国药物与临床,2014,14(5):621-624.
[10]金青青,梁洁,徐晖,等.Box-Behnken响应面法优化龙眼叶总黄酮提取工艺[J].辽宁中医杂志,2017,44(12):2599-2601,2702.
[11]陆海峰,赵进,李琳,等.超声波提取龙眼叶总黄酮及其鉴别[J].微量元素与健康研究,2007(4):48-49.
[12]赵春建,祖元刚,付玉杰,等.匀浆法提取沙棘果中总黄酮的工艺研究[J].林产化学与工业,2006(2):38-40.
[13]蓝峻峰,谢济运,玉澜.纤维素酶辅助提取叶下珠黄酮的工艺优选[J].中国实验方剂学杂志,2011,17(16):26-28.
[14]徐红艳,包怡红.响应面法优化胡桃楸种仁壳总黄酮微波提取工艺[J].食品科学,2013,34(18):32-35.
[15]董翠英,陈娟.超声提取植物药有效成分的研究进展[J].中成药,2017,39(7):1473-1477.
[2]彭新生,周艳星,郑伟清,等.采收季节对龙眼叶中总黄酮含量影响[J].广州化工,2013,41(16):76-77.
[3]Apriyanto D R,Aoki C,Hartati S,et al.Anti-hepatitis C virus activity of a crude extract from longan(Dimocarpus longan Lour.)leaves[J].Japanese Journal of Infectious Diseases,2016,69(3):213.
[4]Wang Y,Li Y,Ma C,et al.Gas exchange,photosystem II photochemistry,and the antioxidant system of longan plant(Dimocarpus longan Lour.)leaves in response to lead(Pb)stress[J].Plant Omics,2016,9(4):240-247.
[5]Liu Y.Antioxidant activity of longan(Dimocarpus longan)barks and leaves[J].African Journal of Biotechnology,2012,11(27):7038-7045.
[6]梁洁,余靓,柳贤福,等.龙眼叶不同提取物降血糖的实验研究[J].时珍国医国药,2013,24(8):2057-2058.
[7]梁洁,滕建北,柳贤福,等.龙眼叶化学成分预试研究[J].中国民族民间医药,2010,19(4):142-143.
[8]陈秋荣.黄酮类化合物药理作用的分析[J].中国实用医药,2012,7(21):254-255.
[9]刘星雨,周敏,孙体健.天然黄酮类化合物的药理活性及分离提取[J].中国药物与临床,2014,14(5):621-624.
[10]金青青,梁洁,徐晖,等.Box-Behnken响应面法优化龙眼叶总黄酮提取工艺[J].辽宁中医杂志,2017,44(12):2599-2601,2702.
[11]陆海峰,赵进,李琳,等.超声波提取龙眼叶总黄酮及其鉴别[J].微量元素与健康研究,2007(4):48-49.
[12]赵春建,祖元刚,付玉杰,等.匀浆法提取沙棘果中总黄酮的工艺研究[J].林产化学与工业,2006(2):38-40.
[13]蓝峻峰,谢济运,玉澜.纤维素酶辅助提取叶下珠黄酮的工艺优选[J].中国实验方剂学杂志,2011,17(16):26-28.
[14]徐红艳,包怡红.响应面法优化胡桃楸种仁壳总黄酮微波提取工艺[J].食品科学,2013,34(18):32-35.
[15]董翠英,陈娟.超声提取植物药有效成分的研究进展[J].中成药,2017,39(7):1473-1477.