基于脂肪酸生物标记法探讨中国沿海六群体绒螯蟹(Eriocheir sensu stricto)的种群遗传
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摘要
水产动物肌肉中的脂肪酸组成可用于评价其种群遗传。本研究通过测定和比较辽河(LH)、黄河(HH)、长江(YZ)、瓯江(OJ)、闽江(MJ)和南流江(NLJ)水系野生绒螯蟹(Eriocheir sensu stricto)肌肉中的脂肪酸组成,进一步分析了6群体间的遗传多样性和亲缘关系。结果显示:(1)在6水系群体中检测出26种脂肪酸,其中C20:5n3、C18:1n9、C22:6n3、C16:0、C20:4n6、C18:2n6和C18:0为主要种类,其总和占总脂肪酸的74%以上;(2)逐步判别分析法筛选出8种判别贡献较大的脂肪酸,分别是C17:0、C18:0、C20:0、C22:1n9、C18:3n3、C18:3n6、C20:2n6和C22:5n3,据此分别建立判别方程,综合判别准确率为98.33%;(3)聚类分析显示:OJ与MJ群体的欧氏距离最小, HH与YZ群体的欧氏距离比OJ与MJ群体的要大,NLJ群体与其他5个群体的欧氏距离相对最大;(4)6群体间脂肪酸含量的变异系数在2.99%—30.00%之间,遗传多样性指数变化幅度在3.2732—3.3204之间。综上,基于脂肪酸生物标记法表明6水系绒螯蟹群体遗传多样性指数均较高,脂肪酸可以有效鉴别6水系野生绒螯蟹种群,整体上南流江群体和其余5群体间相距最远;本研究结果可为绒螯蟹种质资源评价和开发利用等提供基础资料。
The fatty acid composition of aquatic animal muscles can be used to evaluate their population inheritance. In this study, the fatty acid compositions of the six Eriocheir sensu stricto population muscles were measured and compared in phospholipid fatty acids biomarkers, and the six populations were from Liaohe, Huanghe, Changjiang(Yangtze),Oujiang, Minjiang, and Nanliujiang River basins. The genetic diversity and phylogenetic relationship were analyzed among the six Eriocheir s. s. populations. The results show as follows:(1) All the six Eriocheir s. s. populations contained 26 types of fatty acids among which the total volume of seven main fatty acids C20:5 n3, C18:1 n9, C22:6 n3, C16:0, C20:4 n6,C18:2 n6, and C18:0 was over 74%;(2) There were eight fatty acid indices with high contribution to the discrimination of the six Eriocheir s. s. populations, including C17:0, C18:0, C20:0, C22:1 n9, C18:3 n3, C18:3 n6, C20:2 n6, and C22:5 n3.Relevant discrimination equations were established for each Eriocheir s. s. population, the synthetic discriminant accuracy was 98.33%;(3) The results of cluster analysis show that the Euclidean distance between OJ and MJ populations were the closest, and the distance between HH and YZ populations were relatively larger than those of OJ and MJ populations, and the Euclidean distance of NLJ population was the farthest compared with the other five populations;(4) Among the six Eriocheir s. s. populations, the variable coefficients of fatty acid content ranged 2.99%—30.00%, and the genetic diversity indexes varied from 3.2732 to 3.3204. Overall, the genetic diversity indices of the six populations were quite high based on phospholipid fatty acids biomarkers. Fatty acids were effective to identify the six Eriocheir s. s. populations. The phylogenetic relationship of the other five populations were the farthest from NLJ population. The results of this study provide basic information for the evaluation, development and utilization of germplasm resources of Eriocheir s. s.
The fatty acid composition of aquatic animal muscles can be used to evaluate their population inheritance. In this study, the fatty acid compositions of the six Eriocheir sensu stricto population muscles were measured and compared in phospholipid fatty acids biomarkers, and the six populations were from Liaohe, Huanghe, Changjiang(Yangtze),Oujiang, Minjiang, and Nanliujiang River basins. The genetic diversity and phylogenetic relationship were analyzed among the six Eriocheir s. s. populations. The results show as follows:(1) All the six Eriocheir s. s. populations contained 26 types of fatty acids among which the total volume of seven main fatty acids C20:5 n3, C18:1 n9, C22:6 n3, C16:0, C20:4 n6,C18:2 n6, and C18:0 was over 74%;(2) There were eight fatty acid indices with high contribution to the discrimination of the six Eriocheir s. s. populations, including C17:0, C18:0, C20:0, C22:1 n9, C18:3 n3, C18:3 n6, C20:2 n6, and C22:5 n3.Relevant discrimination equations were established for each Eriocheir s. s. population, the synthetic discriminant accuracy was 98.33%;(3) The results of cluster analysis show that the Euclidean distance between OJ and MJ populations were the closest, and the distance between HH and YZ populations were relatively larger than those of OJ and MJ populations, and the Euclidean distance of NLJ population was the farthest compared with the other five populations;(4) Among the six Eriocheir s. s. populations, the variable coefficients of fatty acid content ranged 2.99%—30.00%, and the genetic diversity indexes varied from 3.2732 to 3.3204. Overall, the genetic diversity indices of the six populations were quite high based on phospholipid fatty acids biomarkers. Fatty acids were effective to identify the six Eriocheir s. s. populations. The phylogenetic relationship of the other five populations were the farthest from NLJ population. The results of this study provide basic information for the evaluation, development and utilization of germplasm resources of Eriocheir s. s.
引文
王武,王成辉,马旭洲,2013.河蟹生态养殖.北京:中国农业出版社,59-84
卢义,吴旭干,何杰等,2016.长江、黄河、辽河水系中华绒螯蟹野生扣蟹的形态学及生化组成.中国水产科学,23(2):382-395
成起萱,2011.绒螯蟹的分子遗传变异与进化.上海:上海海洋大学硕士学位论文,31-38
刘青,刘皓,吴旭干等,2015.长江、黄河和辽河水系中华绒螯蟹野生和养殖群体遗传变异的微卫星分析.海洋与湖沼,46(4):958-968
闫龙,宋娜,王俊等,2015.基于线粒体控制区的中华绒螯蟹群体遗传多样性分析.水生生物学报,39(3):615-620
农业部渔业渔政管理局,2017.2017年中国渔业统计年鉴.北京:中国农业出版社,34-38
李晨虹,李思发,1999.中国大陆沿海六水系绒螯蟹(中华绒螯蟹和日本绒螯蟹)群体亲缘关系:形态判别分析.水产学报,23(4):337-342
赵金良,李思发,1999.中国大陆沿海六水系绒螯蟹(中华绒螯蟹和日本绒螯蟹)群体亲缘关系:生化遗传差异分析.水产学报,23(4):331-336
赵恒亮,吴旭干,龙晓文等,2016.长江、黄河和辽河种群中华绒螯蟹雄体成蟹可食组织营养组成的比较.中国水产科学,23(5):1117-1129
彭欣悦,2016.长江口中华绒螯蟹亲蟹遗传多样性及其增殖放流效果评估的研究.上海:上海海洋大学硕士学位论文,24-29
董志国,沈双烨,李晓英等,2013.中国沿海三疣梭子蟹脂肪酸指纹标记的多元分析.水产学报,37(2):192-200
Abdi H,Williams L J,2010.Principal component analysis.Wiley Interdisciplinary Reviews:Computational Statistics,2(4):433-459
Anandakrishnan R,Onufriev A,2008.Analysis of basic clustering algorithms for numerical estimation of statistical averages in biomolecules.Journal of Computational Biology,15(2):165-184
Arslan Y,Hacio?lu B T,2018.Seed fatty acid compositions and chemotaxonomy of wild safflower(Carthamus L.,Asteraceae)species in Turkey.Turkish Journal of Agriculture and Forestry,42(1):45-54
Atta-Krah K,Kindt R,Skilton J N et al,2004.Managing biological and genetic diversity in tropical agroforestry.Agroforestry Systems,61-62(1-3):183-194
Chang Y M,Liang L Q,Ma H T et al,2008.Microsatellite analysis of genetic diversity and population structure of Chinese mitten crab(Eriocheir sinensis).Journal of Genetics and Genomics,35(3):171-176
Chapelle S,1978.Influence of salinity on the lipid composition and the fatty-acid pattern of muscle and hepatopancreas of the Chinese crab Eriocheir sinensis.Archives Internationales de Physiologie et de Biochimie,86(2):393-401
Chen L H,Yang J L,Barnes M D,2014.Effect of natural disturbance on migrations and population dynamics of a catadromous species,Eriocheir japonicus,along the Lo-Mei stream in northern Taiwan.Hydrobiologia,726(1):179-194
Chu K H,Ho H Y,Li C P et al,2003.Molecular phylogenetics of the mitten crab species in Eriocheir,sensu lato(Brachyura:Grapsidae).Journal of Crustacean Biology,23(3):738-746
Dalsgaard J,John M S,Kattner G et al,2003.Fatty acid trophic markers in the pelagic marine environment.Advances in Marine Biology,46:225-340
Figueiredo C,Baptista M,Rosa I C et al,2017.3Dchemoecology and chemotaxonomy of corals using fatty acid biomarkers:Latitude,longitude and depth.Biochemical Systematics and Ecology,70:35-42
Grahl-Nielsen O,Jacobsen A,Christophersen G et al,2010.Fatty acid composition in adductor muscle of juvenile scallops(Pecten maximus)from five Norwegian populations reared in the same environment.Biochemical Systematics Ecology,38(4):478-488
Guo J Y,Ng N K,Dai A et al,1997.The taxonomy of three commercially important species of mitten crabs of the genus Eriocheir de haan,1835(Crustacea:Decapoda:Brachyura:Grapsidae).The Raffles Bulletin of Zoology,45(2):445-476
Herborg L M,Rushton S P,Clare A S et al,2003.Spread of the Chinese mitten crab(Eriocheir sinensis H.Milne Edwards)in continental Europe:analysis of a historical data set.Hydrobiologia,503(1-3):21-28
Imbs A B,Demidkova D A,Dautova T N et al,2009.Fatty acid biomarkers of symbionts and unusual inhibition of tetracosapolyenoic acid biosynthesis in corals(Octocorallia).Lipids,44(4):325-335
Jarvis B D W,Tighe S W,1994.Rapid identification of Rhizobium species based on cellular fatty acid analysis.Plant and Soil,161(1):31-41
Li G,Shen Q,Xu Z X,1993.Morphometric and biochemical genetic variation of the mitten crab,Eriocheir,in southern China.Aquaculture,111(1-4):103-115
Ma H T,Chang Y M,Yu D M et al,2007.Microsatellite variations among four populations of Eriocheir sinensis.Zoological Research,28(2):126-133
Morrison W R,Smith L M,1964.Preparation of fatty acid methyl esters and dimethylacetals from lipids with boron fluoride-methanol.Journal of Lipid Research,5(4):600-608
Neira R,Lhorente J P,Araneda C et al,2004.Studies on carcass quality traits in two populations of Coho salmon(Oncorhynchus kisutch):phenotypic and genetic parameters.Aquaculture,241(1-4):117-131
Ricardo F,Pimentel T,Moreira A S P et al,2015.Potential use of fatty acid profiles of the adductor muscle of cockles(Cerastoderma edule)for traceability of collection site.Scientific Reports,5(120):11125
Santos I C,Smuts J,Choi W S et al,2018.Analysis of bacterial FAMEs using gas chromatography-vacuum ultraviolet spectroscopy for the identification and discrimination of bacteria.Talanta,182:536-543
Shin P K S,Yip K M,Xu W Z et al,2008.Fatty acid as markers to demonstrating trophic relationships among diatoms,rotifers and green-lipped mussels.Journal of Experimental Marine Biology and Ecology,357(1):75-84
Sui L Y,Sun H X,Wu X G et al,2011a.Effect of dietary HUFAon tissue fatty acid composition and reproductive performance of Chinese mitten crab Eriocheir sinensis(H.Milne-Edwards)broodstock.Aquaculture International,19(2):269-282
Sui L Y,Wille M,Cheng Y X et al,2011b.Larviculture techniques of Chinese mitten crab Eriocheir sinensis.Aquaculture,315(1-2):16-19
Sui L Y,Zhang F M,Wang X M et al,2009.Genetic diversity and population structure of the Chinese mitten crab Eriocheir sinensis in its native range.Marine Biology,156(8):1573-1583
Tang B P,Zhou K Y,Song D X et al,2003.Molecular systematics of the Asian mitten crabs,genus Eriocheir(Crustacea:Brachyura).Molecular Phylogenetics and Evolution,29(2):309-316
Wang C H,Li C H,Li S F,2008.Mitochondrial DNA-inferred population structure and demographic history of the mitten crab(Eriocheir sensu stricto)found along the coast of mainland China.Molecular Ecology,17(15):3515-3527
Wang G Z,Kong X H,Wang K J et al,2007.Variation of specific proteins,mitochondria and fatty acid composition in gill of Scylla serrata(Crustacea,Decapoda)under low temperature adaptation.Journal of Experimental Marine Biology and Ecology,352(1):129-138
Wu X G,Wang Z K,Cheng Y X et al,2011.Effects of dietary phospholipids and highly unsaturated fatty acids on the precocity,survival,growth and hepatic lipid composition of juvenile Chinese mitten crab,Eriocheir sinensis(H.Milne-Edwards).Aquaculture Research,42(3):457-468
Xu J W,Chu K H,2012.Genome scan of the mitten crab Eriocheir sensu stricto in East Asia:population differentiation,hybridization and adaptive speciation.Molecular Phylogenetics and Evolution,64(1):118-129
Zhang C,Li Q Q,Wu X G et al,2017.Genetic diversity and genetic structure of farmed and wild Chinese mitten crab(Eriocheir sinensis)populations from three major basins by mitochondrial DNA COI and Cyt b gene sequences.Mitochondrial DNA Part A,29(7):1081-1089
卢义,吴旭干,何杰等,2016.长江、黄河、辽河水系中华绒螯蟹野生扣蟹的形态学及生化组成.中国水产科学,23(2):382-395
成起萱,2011.绒螯蟹的分子遗传变异与进化.上海:上海海洋大学硕士学位论文,31-38
刘青,刘皓,吴旭干等,2015.长江、黄河和辽河水系中华绒螯蟹野生和养殖群体遗传变异的微卫星分析.海洋与湖沼,46(4):958-968
闫龙,宋娜,王俊等,2015.基于线粒体控制区的中华绒螯蟹群体遗传多样性分析.水生生物学报,39(3):615-620
农业部渔业渔政管理局,2017.2017年中国渔业统计年鉴.北京:中国农业出版社,34-38
李晨虹,李思发,1999.中国大陆沿海六水系绒螯蟹(中华绒螯蟹和日本绒螯蟹)群体亲缘关系:形态判别分析.水产学报,23(4):337-342
赵金良,李思发,1999.中国大陆沿海六水系绒螯蟹(中华绒螯蟹和日本绒螯蟹)群体亲缘关系:生化遗传差异分析.水产学报,23(4):331-336
赵恒亮,吴旭干,龙晓文等,2016.长江、黄河和辽河种群中华绒螯蟹雄体成蟹可食组织营养组成的比较.中国水产科学,23(5):1117-1129
彭欣悦,2016.长江口中华绒螯蟹亲蟹遗传多样性及其增殖放流效果评估的研究.上海:上海海洋大学硕士学位论文,24-29
董志国,沈双烨,李晓英等,2013.中国沿海三疣梭子蟹脂肪酸指纹标记的多元分析.水产学报,37(2):192-200
Abdi H,Williams L J,2010.Principal component analysis.Wiley Interdisciplinary Reviews:Computational Statistics,2(4):433-459
Anandakrishnan R,Onufriev A,2008.Analysis of basic clustering algorithms for numerical estimation of statistical averages in biomolecules.Journal of Computational Biology,15(2):165-184
Arslan Y,Hacio?lu B T,2018.Seed fatty acid compositions and chemotaxonomy of wild safflower(Carthamus L.,Asteraceae)species in Turkey.Turkish Journal of Agriculture and Forestry,42(1):45-54
Atta-Krah K,Kindt R,Skilton J N et al,2004.Managing biological and genetic diversity in tropical agroforestry.Agroforestry Systems,61-62(1-3):183-194
Chang Y M,Liang L Q,Ma H T et al,2008.Microsatellite analysis of genetic diversity and population structure of Chinese mitten crab(Eriocheir sinensis).Journal of Genetics and Genomics,35(3):171-176
Chapelle S,1978.Influence of salinity on the lipid composition and the fatty-acid pattern of muscle and hepatopancreas of the Chinese crab Eriocheir sinensis.Archives Internationales de Physiologie et de Biochimie,86(2):393-401
Chen L H,Yang J L,Barnes M D,2014.Effect of natural disturbance on migrations and population dynamics of a catadromous species,Eriocheir japonicus,along the Lo-Mei stream in northern Taiwan.Hydrobiologia,726(1):179-194
Chu K H,Ho H Y,Li C P et al,2003.Molecular phylogenetics of the mitten crab species in Eriocheir,sensu lato(Brachyura:Grapsidae).Journal of Crustacean Biology,23(3):738-746
Dalsgaard J,John M S,Kattner G et al,2003.Fatty acid trophic markers in the pelagic marine environment.Advances in Marine Biology,46:225-340
Figueiredo C,Baptista M,Rosa I C et al,2017.3Dchemoecology and chemotaxonomy of corals using fatty acid biomarkers:Latitude,longitude and depth.Biochemical Systematics and Ecology,70:35-42
Grahl-Nielsen O,Jacobsen A,Christophersen G et al,2010.Fatty acid composition in adductor muscle of juvenile scallops(Pecten maximus)from five Norwegian populations reared in the same environment.Biochemical Systematics Ecology,38(4):478-488
Guo J Y,Ng N K,Dai A et al,1997.The taxonomy of three commercially important species of mitten crabs of the genus Eriocheir de haan,1835(Crustacea:Decapoda:Brachyura:Grapsidae).The Raffles Bulletin of Zoology,45(2):445-476
Herborg L M,Rushton S P,Clare A S et al,2003.Spread of the Chinese mitten crab(Eriocheir sinensis H.Milne Edwards)in continental Europe:analysis of a historical data set.Hydrobiologia,503(1-3):21-28
Imbs A B,Demidkova D A,Dautova T N et al,2009.Fatty acid biomarkers of symbionts and unusual inhibition of tetracosapolyenoic acid biosynthesis in corals(Octocorallia).Lipids,44(4):325-335
Jarvis B D W,Tighe S W,1994.Rapid identification of Rhizobium species based on cellular fatty acid analysis.Plant and Soil,161(1):31-41
Li G,Shen Q,Xu Z X,1993.Morphometric and biochemical genetic variation of the mitten crab,Eriocheir,in southern China.Aquaculture,111(1-4):103-115
Ma H T,Chang Y M,Yu D M et al,2007.Microsatellite variations among four populations of Eriocheir sinensis.Zoological Research,28(2):126-133
Morrison W R,Smith L M,1964.Preparation of fatty acid methyl esters and dimethylacetals from lipids with boron fluoride-methanol.Journal of Lipid Research,5(4):600-608
Neira R,Lhorente J P,Araneda C et al,2004.Studies on carcass quality traits in two populations of Coho salmon(Oncorhynchus kisutch):phenotypic and genetic parameters.Aquaculture,241(1-4):117-131
Ricardo F,Pimentel T,Moreira A S P et al,2015.Potential use of fatty acid profiles of the adductor muscle of cockles(Cerastoderma edule)for traceability of collection site.Scientific Reports,5(120):11125
Santos I C,Smuts J,Choi W S et al,2018.Analysis of bacterial FAMEs using gas chromatography-vacuum ultraviolet spectroscopy for the identification and discrimination of bacteria.Talanta,182:536-543
Shin P K S,Yip K M,Xu W Z et al,2008.Fatty acid as markers to demonstrating trophic relationships among diatoms,rotifers and green-lipped mussels.Journal of Experimental Marine Biology and Ecology,357(1):75-84
Sui L Y,Sun H X,Wu X G et al,2011a.Effect of dietary HUFAon tissue fatty acid composition and reproductive performance of Chinese mitten crab Eriocheir sinensis(H.Milne-Edwards)broodstock.Aquaculture International,19(2):269-282
Sui L Y,Wille M,Cheng Y X et al,2011b.Larviculture techniques of Chinese mitten crab Eriocheir sinensis.Aquaculture,315(1-2):16-19
Sui L Y,Zhang F M,Wang X M et al,2009.Genetic diversity and population structure of the Chinese mitten crab Eriocheir sinensis in its native range.Marine Biology,156(8):1573-1583
Tang B P,Zhou K Y,Song D X et al,2003.Molecular systematics of the Asian mitten crabs,genus Eriocheir(Crustacea:Brachyura).Molecular Phylogenetics and Evolution,29(2):309-316
Wang C H,Li C H,Li S F,2008.Mitochondrial DNA-inferred population structure and demographic history of the mitten crab(Eriocheir sensu stricto)found along the coast of mainland China.Molecular Ecology,17(15):3515-3527
Wang G Z,Kong X H,Wang K J et al,2007.Variation of specific proteins,mitochondria and fatty acid composition in gill of Scylla serrata(Crustacea,Decapoda)under low temperature adaptation.Journal of Experimental Marine Biology and Ecology,352(1):129-138
Wu X G,Wang Z K,Cheng Y X et al,2011.Effects of dietary phospholipids and highly unsaturated fatty acids on the precocity,survival,growth and hepatic lipid composition of juvenile Chinese mitten crab,Eriocheir sinensis(H.Milne-Edwards).Aquaculture Research,42(3):457-468
Xu J W,Chu K H,2012.Genome scan of the mitten crab Eriocheir sensu stricto in East Asia:population differentiation,hybridization and adaptive speciation.Molecular Phylogenetics and Evolution,64(1):118-129
Zhang C,Li Q Q,Wu X G et al,2017.Genetic diversity and genetic structure of farmed and wild Chinese mitten crab(Eriocheir sinensis)populations from three major basins by mitochondrial DNA COI and Cyt b gene sequences.Mitochondrial DNA Part A,29(7):1081-1089