曼氏裂头蚴病动物模型的建立及诊治技术研究Ⅰ小鼠动物模型建立及感染后血清特异性IgG抗体变化
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摘要
目的建立曼氏裂头蚴感染小鼠动物模型,并观察感染后小鼠血常规及血清中特异性IgG抗体变化。方法从青蛙体内检获曼氏裂头蚴,以口服灌胃法感染昆明小鼠25只(3条/只)。于感染前2 d和感染后第2、7、14、21、28、35、42、49天采集小鼠血液,检测血常规(白细胞总数、红细胞总数、血红蛋白、血小板总数)及血清中特异性IgG抗体(ELISA法);于感染后第49天处死全部小鼠,解剖并仔细查找小鼠体内的曼氏裂头蚴。结果小鼠感染曼氏裂头蚴后,白细胞总数在第2天明显升高;血清中特异性IgG抗体于感染后第14天开始检出,于第21天从全部小鼠检出。在小鼠多个组织器官检出曼氏裂头蚴,其中以皮下肌肉多见。结论成功建立小鼠感染曼氏裂头蚴动物模型,该法造模简单、经济。白细胞及血清中特异性抗体检测可作为曼氏裂头蚴的辅助诊断方法。
Objective To establish an animal model of Sparganum mansoni(plerocercoid larva of S. mansoni) infection in mice and observe the changes of blood routine examinations and serum anti-sparganum antibody levels after the infection. Methods The spargana tapeworms were collected from frogs, and 25 Kunming mice were orally infected with the Sparganum tapeworms(3 tapeworms/mouse). Two days before the infection and 2,7, 14, 21, 28, 35, 42 days and 49 days after the infection,the peripheral blood samples of mice were collected for the blood routine examinations and the detections of anti-S. mansoni IgG antibody with ELISA. Forty-nine days after the infection, all the mice were sacrificed to find out the Sparganum tapeworms in the bodies of mice. Results The count of the total white blood cells was significantly elevated on the second day of the mice infected with Sparganum. The serum anti-Sparganum antibody was detected on the 14th day of the infection in some mice, and on the 21st day of the infection, the serum anti-Sparganum antibody was detected in all the mice. After the mice were sacrificed,the Sparganum tapeworms were found out in many tissues and organs, and especially in the subcutaneous tissues and muscle.Conclusion The establishment of animal model of Sparganum infection is successful in mice with the oral method, and white blood cells and serum specific IgG antibody detection can be used as auxiliary diagnosis methods of S. mansoni infection.
Objective To establish an animal model of Sparganum mansoni(plerocercoid larva of S. mansoni) infection in mice and observe the changes of blood routine examinations and serum anti-sparganum antibody levels after the infection. Methods The spargana tapeworms were collected from frogs, and 25 Kunming mice were orally infected with the Sparganum tapeworms(3 tapeworms/mouse). Two days before the infection and 2,7, 14, 21, 28, 35, 42 days and 49 days after the infection,the peripheral blood samples of mice were collected for the blood routine examinations and the detections of anti-S. mansoni IgG antibody with ELISA. Forty-nine days after the infection, all the mice were sacrificed to find out the Sparganum tapeworms in the bodies of mice. Results The count of the total white blood cells was significantly elevated on the second day of the mice infected with Sparganum. The serum anti-Sparganum antibody was detected on the 14th day of the infection in some mice, and on the 21st day of the infection, the serum anti-Sparganum antibody was detected in all the mice. After the mice were sacrificed,the Sparganum tapeworms were found out in many tissues and organs, and especially in the subcutaneous tissues and muscle.Conclusion The establishment of animal model of Sparganum infection is successful in mice with the oral method, and white blood cells and serum specific IgG antibody detection can be used as auxiliary diagnosis methods of S. mansoni infection.
引文
[1] Bai J,He ZY,Liu GN,et al. Bronchial sparganosis mansoni accompanied by abnormal hyperplasia diagnosed by bronchoscopy[J]. Chin Med J(Engl),2012,125(17):3183-3187.
[2]梁培,尹飞飞,夏乾峰,等.曼氏迭宫绦虫翻译控制肿瘤蛋白(TCTP)的真核表达、虫体组织和亚细胞定位及免疫保护效果评价[J].中国病原生物学杂志,2017,12(7):647-650,653.
[3]杨光大,肖嘉杰,龚世平,等.曼氏裂头蚴病诊断方法及其在蛇蛙的应用研究进展[J].动物医学进展,2015,36(9):101-104.
[4] Lu G,Shi DZ,Lu YJ,et al. Retrospective epidemiological analysis of sparganosis in mainland China from 1959 to 2012[J]. Epidemiol Infect,2014,142(12):2654-2661.
[5]裘明华,裘明德.人裂头蚴病和无头蚴病:Ⅱ.病理学、临床、流行病学及控制的过去和现在[J].中国寄生虫学与寄生虫病杂志,2009,27(3):251-260.
[6]王越,叶丽萍,孙亚维,等.曼氏裂头蚴可溶性抗原DIGFA法快速检测特异性抗体的潜在诊断价值[J].中国病原生物学杂志,2006,1(4):260-262.
[7]蔺西萌,张红卫,刘长军,等.曼氏迭宫绦虫动物模型的建立和生活史观察[J].中国人兽共患病学报,2011,27(2):152-153.
[8]姜鹏,张玺,祁欣,等.裂头蚴感染小鼠模型的建立[J].中国病原生物学杂志,2015,10(3):247-248,271.
[9]王付民,龚世平,邓燕忠.广东省食用蛇蛙类曼氏裂头蚴病的流行与防控[M].广州:华南理工大学出版社,2015:27-30.
[10]杨光大,肖嘉杰,王付民,等.我国蛙类曼氏裂头蚴病的研究概况及防控措施[J].广东林业科技,2013,29(2):62-67.
[11]周庆安,全琛宇,曾芸,等.广西南宁市市售蛙和蛇感染裂头蚴情况的调查[J].动物医学进展,2013,34(11):126-128.
[12]仝德胜,张英,胡楠楠,等.无锡地区农贸市场青蛙曼氏裂头蚴感染情况调查[J].实验与检验医学,2017,35(6):965-966.
[13] Zhang X,Wang H,Cui J,et al. Characterisation of the relationshipbetween Spirometra erinaceieuropaei and Diphyllobothrium speciesusing complete cytb and cox1 genes[J]. Infect Genet Evol,2015,35:1-8.
[14]裘学丽,陈艳,蒋红涛,等.小鼠感染曼氏裂头蚴后体内虫体分布与血清抗体水平变化[J].中国血吸虫病防治杂志,2010,22(3):272-274.
[15]李楠,崔晶,王书伟,等.曼氏裂头蚴感染小鼠血清IgG抗体动态变化[J].中国人兽共患病学报,2010,26(9):837-839.
[16]蒋红涛,陈艳,唐贵文.感染曼氏裂头蚴小鼠的组织病理学观察[J].中国人兽共患病学报,2010,26(3):226-228.
[17]兰婧,陈艳,陶梓璐,等.实验小鼠体内曼氏裂头蚴移行途径及分布的研究[J].四川动物,2014,3(6):918-922.
[18] Cui J,Li N,Wang ZQ,et al. Serodiagnosis of experimental sparganum infections of mice and human sparganosis by ELISA usingES antigens of Spirometra mansoni spargana[J]. Parasitol Res,2011,108(6):1551-1556.
[2]梁培,尹飞飞,夏乾峰,等.曼氏迭宫绦虫翻译控制肿瘤蛋白(TCTP)的真核表达、虫体组织和亚细胞定位及免疫保护效果评价[J].中国病原生物学杂志,2017,12(7):647-650,653.
[3]杨光大,肖嘉杰,龚世平,等.曼氏裂头蚴病诊断方法及其在蛇蛙的应用研究进展[J].动物医学进展,2015,36(9):101-104.
[4] Lu G,Shi DZ,Lu YJ,et al. Retrospective epidemiological analysis of sparganosis in mainland China from 1959 to 2012[J]. Epidemiol Infect,2014,142(12):2654-2661.
[5]裘明华,裘明德.人裂头蚴病和无头蚴病:Ⅱ.病理学、临床、流行病学及控制的过去和现在[J].中国寄生虫学与寄生虫病杂志,2009,27(3):251-260.
[6]王越,叶丽萍,孙亚维,等.曼氏裂头蚴可溶性抗原DIGFA法快速检测特异性抗体的潜在诊断价值[J].中国病原生物学杂志,2006,1(4):260-262.
[7]蔺西萌,张红卫,刘长军,等.曼氏迭宫绦虫动物模型的建立和生活史观察[J].中国人兽共患病学报,2011,27(2):152-153.
[8]姜鹏,张玺,祁欣,等.裂头蚴感染小鼠模型的建立[J].中国病原生物学杂志,2015,10(3):247-248,271.
[9]王付民,龚世平,邓燕忠.广东省食用蛇蛙类曼氏裂头蚴病的流行与防控[M].广州:华南理工大学出版社,2015:27-30.
[10]杨光大,肖嘉杰,王付民,等.我国蛙类曼氏裂头蚴病的研究概况及防控措施[J].广东林业科技,2013,29(2):62-67.
[11]周庆安,全琛宇,曾芸,等.广西南宁市市售蛙和蛇感染裂头蚴情况的调查[J].动物医学进展,2013,34(11):126-128.
[12]仝德胜,张英,胡楠楠,等.无锡地区农贸市场青蛙曼氏裂头蚴感染情况调查[J].实验与检验医学,2017,35(6):965-966.
[13] Zhang X,Wang H,Cui J,et al. Characterisation of the relationshipbetween Spirometra erinaceieuropaei and Diphyllobothrium speciesusing complete cytb and cox1 genes[J]. Infect Genet Evol,2015,35:1-8.
[14]裘学丽,陈艳,蒋红涛,等.小鼠感染曼氏裂头蚴后体内虫体分布与血清抗体水平变化[J].中国血吸虫病防治杂志,2010,22(3):272-274.
[15]李楠,崔晶,王书伟,等.曼氏裂头蚴感染小鼠血清IgG抗体动态变化[J].中国人兽共患病学报,2010,26(9):837-839.
[16]蒋红涛,陈艳,唐贵文.感染曼氏裂头蚴小鼠的组织病理学观察[J].中国人兽共患病学报,2010,26(3):226-228.
[17]兰婧,陈艳,陶梓璐,等.实验小鼠体内曼氏裂头蚴移行途径及分布的研究[J].四川动物,2014,3(6):918-922.
[18] Cui J,Li N,Wang ZQ,et al. Serodiagnosis of experimental sparganum infections of mice and human sparganosis by ELISA usingES antigens of Spirometra mansoni spargana[J]. Parasitol Res,2011,108(6):1551-1556.