双水相法回收可利霉素生产废水中的螺旋霉素
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摘要
采用浊点滴定法测定了乙醇-K2HPO4双水相体系的双节线相图,并使用经验方程对数据进行拟合,建立了乙醇-K2HPO4双水相体系萃取螺旋霉素的方法,系统考察了K2HPO4质量分数、乙醇质量分数、螺旋霉素初始浓度、萃取温度和体系pH对分配系数和萃取率的影响。结果表明:双水相萃取螺旋霉素是自发进行、吸热熵增的过程。当K2HPO4质量分数为总体系的20%、乙醇质量分数为总体系的16%、萃取温度为25℃、体系pH=9.5时,分配系数达到47.52,萃取率达到97.97%。
Binodal curve of ethanol-K2 HPO4 aqueous two-phase system(ATPS) was measured by the turbidimetric titration method. Empirical equations were used to correlate the bimodal curves. A method for separation of spiramycin by ethanol-K2 HPO4 aqueous two-phase system extraction was established. Parameters affecting the partition coefficient and extraction efficiency such as mass fraction of K2 HPO4, mass fraction of ethanol, initial concentration of spiramycin, extraction temperature and pH of the system were investigated. The results demonstrated that the extraction of spiramycin was a spontaneous, endothermic and entropy increasing process. When the K2 HPO4 mass fraction was 20% of the total system, the ethanol mass fraction was 16% of the total system, the extraction temperature was 25 ℃, and the pH of the system was 9.5, the partition coefficient and extraction efficiency of spiramycin reached 47.52 and 97.97%, respectively.
Binodal curve of ethanol-K2 HPO4 aqueous two-phase system(ATPS) was measured by the turbidimetric titration method. Empirical equations were used to correlate the bimodal curves. A method for separation of spiramycin by ethanol-K2 HPO4 aqueous two-phase system extraction was established. Parameters affecting the partition coefficient and extraction efficiency such as mass fraction of K2 HPO4, mass fraction of ethanol, initial concentration of spiramycin, extraction temperature and pH of the system were investigated. The results demonstrated that the extraction of spiramycin was a spontaneous, endothermic and entropy increasing process. When the K2 HPO4 mass fraction was 20% of the total system, the ethanol mass fraction was 16% of the total system, the extraction temperature was 25 ℃, and the pH of the system was 9.5, the partition coefficient and extraction efficiency of spiramycin reached 47.52 and 97.97%, respectively.
引文
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[17]Ma Jingjing(马晶晶).Construction of small molecular watermiscible alcohol-salt aqueous two phase system and application on the extraction of plant pigment[D].Zhenjiang:Jiangsu University of Science and Technology(江苏科技大学),2013.
[18]Feng Wenzheng(冯闻铮),Qi Pingyan(亓平言),Zhou Ti(周倜),et al.Study on the degradation kinetics of spiramycin in acid and alkaline solutions[J].Acta Pharmaceutica Sinica(药学学报),1997,32(12):934-937.
[2]Wang Yun(王赟).Construction,phase separation mechanism and application of small molecular water-miscible alcohol-salt aqueous two phase system[D].Zhenjiang:Jiangsu University(江苏大学),2010.
[3]Mokhtari A,Shahriari S.Extraction of cephalexin using aqueous two-phase systems composed of cholinium chloride and K3PO4[J].Quarterly Journal of Applied Chemical Research,2017,11(1):21-33.
[4]Zhao Yunshan(赵云灿),Xue Xuedong(薛学东),Zhao Bo(赵波),et al.Extraction of cephalosporin c with a new type aqueous two-phase system[J].Chinese Journal of Pharmaceuticals(中国医药工业杂志),2012,43(5):337-339,371.
[5]Guan Weisheng(关卫省),Deng Jianghua(邓江华),Han Juan(韩娟).Extraction of doxycycline hydrochloride using anhydrous alcohol and acetone binary aqueous two phase system[J].Applied Chemical Industry(应用化工),2012,41(6):992-995,999.
[6]Mündges J,Zierow J,Zeiner T.Experiment and simulation of an aqueous two-phase extraction process for the purification of a monoclonal antibody[J].Chemical Engineering and Processing:Process Intensification,2015,95(9):31-42.
[7]Khayati G,Gilani H G,Safari Keyvani Z.Extraction of Cu(II)ions from aqueous media using peg/sulphate salt aqueous two-phase system[J].Separation Science and Technology,2016,51(4):601-608.
[8]Cienfuegos N E C,Santos P L,García A R,et al.Integrated process for purification of capsaicin using aqueous two-phase systems based on ethanol[J].Food and Bioproducts Processing,2017,106(11):1-10.
[9]Chen L L,Li F F,Tan Z J.Chiral separation ofα-cyclohexylmandelic acid enantiomers using ionic liquid/salt aqueous two-phase system[J].Chemical Papers,2015,69(11):1465-1472.
[10]Qin Dehua(秦德华),Pan Jie(潘杰),Gao Hong(高红).Study on the extraction of propionylspiramycin by aqueous two phase system[J].Chinese Journal of Antibiotics(中国抗生素杂志),1998,23(2):67-70.
[11]Li Y J,Wu Y Y,Chen K,et al.Partition behavior of spiramycin in an aqueous two-phase system based on polyethylene glycol and sulfates[J].Separation Science and Technology,2018,53(3):496-502.
[12]Li Z,Teng H,Xiu Z.Extraction of 1,3-propanediol from glycerol-based fermentation broths with methanol/phosphate aqueous two-phase system[J].Process Biochemistry,2011,46(2):586-591.
[13]Chen Zhiping(陈志萍),Wu Xingyan(邬行彦),Ou Jie(欧杰).The distribution coefficient of spiramycin extraction from aqueous phase using butyl acetate and its calculation nomograph[J].Chinese Journal of Antibiotics(中国抗生素杂志),1991,16(5):334-338.
[14]Merchuk J C,Andrews B A,Asenjo J A.Aqueous two-phase systems for protein separation:Studies on phase inversion[J].Journal of Chromatography B:Biomedical Sciences and Applications,1998,711(1):285-293.
[15]Regupathi I,Murugesan S,Govindarajan R,et al.Liquid-liquid equilibrium of poly(ethylene glycol)6000+triammonium citrate+water systems at different temperatures[J].Journal of Chemical and Engineering Data,2009,54(3):1094-1097.
[16]Han J,Wang Y,Li Y F,et al.Equilibrium phase behavior of aqueous two-phase systems containing 1-alkyl-3-methylimidazolium tetrafluoroborate and ammonium tartrate at different temperatures:Experimental determination and correlation[J].Journal of Chemical and Engineering Data,2011,56(9):3679-3687.
[17]Ma Jingjing(马晶晶).Construction of small molecular watermiscible alcohol-salt aqueous two phase system and application on the extraction of plant pigment[D].Zhenjiang:Jiangsu University of Science and Technology(江苏科技大学),2013.
[18]Feng Wenzheng(冯闻铮),Qi Pingyan(亓平言),Zhou Ti(周倜),et al.Study on the degradation kinetics of spiramycin in acid and alkaline solutions[J].Acta Pharmaceutica Sinica(药学学报),1997,32(12):934-937.