微波辅助水酶法优化红枣中环磷酸腺苷(cAMP)提取工艺及其抗疲劳作用
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摘要
以新疆红枣为原料提取环磷酸腺苷(cyclic adenosine 3’,5’-monophosphate,cAMP),通过响应面法优化微波辅助复配酶法提取cAMP工艺,并建立回归模型;同时研究cAMP提取物对D-半乳糖诱导衰老模型小鼠的游泳耐力的影响。结果表明,最佳提取工艺为复配酶(纤维素酶-半纤维素酶-果胶酶)质量比1∶1∶1、复配酶添加量1.5%(质量分数)、酶解温度31.3℃、酶解时间20 min、微波功率500 W,在此条件下cAMP提取量为560.9μg/g;新疆红枣cAMP提取物能延长小鼠力竭游泳时间,增加小鼠游泳耐力,说明cAMP对小鼠具有很好的抗疲劳作用。新疆红枣作为cAMP的提取原料在食品和医药工业中具有较好的应用前景。
Response surface methodology was used to optimize the microwave-assisted aqueous enzymatic extraction of cyclic adenosine 3',5'-monophosphate(cAMP) from Chinese jujube(Zizyphus jujuba Mill.) grown in Xinjiang.The antifatigue effect of the cAMP extracted was investigated.The optimal extraction conditions was found as follows: enzyme concentration 1.5%(a mixture of cellulase,hemicellulase and pectinase,1:1:1(m/m)),temperature 31.3 ℃,time 20 min and microwave power 500 W.Under these conditions,the yield of cAMP was 560.9 μg/g.The results showed that the cAMP could extend exhaustive swimming time and enhance swimming endurance in mice,suggesting good anti-fatigue effect.Therefore,cAMP holds great promise for its application in the food and pharmaceutical industry.
Response surface methodology was used to optimize the microwave-assisted aqueous enzymatic extraction of cyclic adenosine 3',5'-monophosphate(cAMP) from Chinese jujube(Zizyphus jujuba Mill.) grown in Xinjiang.The antifatigue effect of the cAMP extracted was investigated.The optimal extraction conditions was found as follows: enzyme concentration 1.5%(a mixture of cellulase,hemicellulase and pectinase,1:1:1(m/m)),temperature 31.3 ℃,time 20 min and microwave power 500 W.Under these conditions,the yield of cAMP was 560.9 μg/g.The results showed that the cAMP could extend exhaustive swimming time and enhance swimming endurance in mice,suggesting good anti-fatigue effect.Therefore,cAMP holds great promise for its application in the food and pharmaceutical industry.
引文
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[29]王郅媛,王友升,李丽萍.抗衰老研究新模型-酵母细胞活性氧代谢研究进展[J].食品科学,2012,33(7):354-358.
[30]杨婷,张冲,陈清轩.衰老机制研究进展[J].中国生物工程杂志,2005,25(3):6-11.
[31]陈艳珍.复合微量营养素对衰老雌性小鼠抗疲劳和抗氧化作用的研究[J].山东理工大学学报(自然科学版),2009,23(5):1-4.
[32]赵凡凡,周玉枝,高丽,等.D-半乳糖致衰老大鼠模型的研究进展[J].药学学报,2017,52(3):347-354.
[2]ZOZIO S,SERVENT A,CAZAL G.Changes in antioxidant activity during the ripening of jujube(Ziziphus mauritiana Lamk)[J].Food Chemistry,2015,150(2):448-4 56.DOI:10.1016/j.foodchem.2013.11.022.
[3]刘庆春,毕珣.枣环磷酸腺苷提取液对小鼠抗疲劳作用的药效学研究[J].肠外与肠内营养,2015,22(1):46-48.DOI:10.16151/j.1007-810x.2015.01.014.
[4]PATRICK O,BASTIAN H,BORIS F.G-protein coupled receptor6 deficiency alters striatal dopamine and cAMP concentrations and reduces dyskinesia in amouse model of Parkinson’s disease[J].Experimental Neurology,2014,257:1-9.DOI:10.1016/j.expneurol.2014.04.010.
[5]赵爱玲,李登科,王永康.枣树不同品种、发育时期和器官的cAMP和cGMP含量研究[J].园艺学报,2009,36(8):1134-1139.DOI:10.16420/j.issn.0513-353x.2009.08.007.
[6]杨芳媛,赵智慧,赵锦,等.枣中环磷酸腺苷的研究进展[J].塔里木大学学报,2016,28(3):119-122.
[7]WANG W,ZHANG X,ZHENG J Q,et al.High glucose stimulates adipogenic and inhibits osteogenic differentiation in MG-63cells through cAMP/protein kinase A/extra cellular signal-regulated kinase pathway[J].Molecular and Cellular Biochemistry,2010,338(1/2):115-122.DOI:10.1007/s11010-009-0344-6.
[8]KERFANT B G,ROSE R A,SUN H,et al.Phosphoinositide 3-kinase gamma regulates cardiac contractility by locally controlling cyclic adenosine monophosphate levels review[J].Trends in Cardiovascular Medicine,2006,16(7):250-256.DOI:10.1016/j.tcm.2006.04.006.
[9]SUBR A H,WALTER U,GAMBAR Y S.Differential regulation of platelet inhibition by cGMP and cAMP dependent protein kinases[J].BMC Pharmacology and Toxicology,2013,14(S1):69.DOI:10.1186/2050-6511-14-S1-P69.
[10]樊君,石奇,尚红伟.环磷酸腺苷的研究进展[J].中国现代应用药学,2005,22(增刊1):597-599.
[11]CLAIRE Y,ZHAO C Y,JOSEPH L,et al.Roles of phosphodiesterases in the regulation of the cardiac cyclic nucleotide cross-talk signaling network[J].Journal of Molecular and Cellular Cardiology,2016,91:215-227.DOI:10.1016/j.yjmcc.2016.01.004.
[12]周向辉,王娜.微波-超声波联合提取枣中环磷酸腺苷的工艺研究[J].食品科学,2009,30(18):196-201.
[13]史红梅,尹卓容,蒋文强.高压液相色谱法测定金丝小枣环磷酸腺苷的含量[J].食品科学,2006,27(5):216-218.
[14]王立霞.微波辅助提取和田玉枣环磷酸腺苷的工艺及与其他方法比较[J].食品与生物技术学报,2014,33(3):293-300.
[15]CHANG Y M,DU J J.Extraction and separation of cAMP from Zizyphus jujuba fruit[J].Acta Hortic,2009,840:541-546.DOI:10.17660/Acta Hortic.2009.840.77.
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[17]蒋劢博,王强,李建贵,等.响应面法优化红枣中环磷酸腺苷(cAMP)超声提取工艺[J].中国食品学报,2014,14(1):114-120.
[18]CHENG Z,SONG H,YANG Y,et al.Optimization of microwave assisted enzymatic extraction of polysaccharides from the fruit of Schisandra chinensis Baill[J].International Journal of Biological Macromolecules,2015,76(5):161-168.DOI:10.1016/j.ijbiomac.2015.01.048.
[19]ZHANG G,HU M,HE L,et al.Optimization of microwave-assisted enzymatic extraction of polyphenols from waste peanut shells and evaluation of its antioxidant and antibacterial activities in vitro[J].Food and Bioproducts Processing,2013,91(2):158-168.DOI:10.1016/j.fbp.2012.09.003.
[20]WU D,GAO T,YANG H,et al.Simultaneous microwave/ultrasonicassisted enzymatic extraction of antioxidant ingredients from Nitraria tangutorun Bobr.juice by-products[J].Industrial Crops and Products,2015,66(4):229-238.DOI:10.1016/j.indcrop.2014.12.054.
[21]于丽秀,华小黎,陈东生,等.微波辅助提取菝葜有效成分及其对钙调神经磷酸酶活性的作用[J].中国医院药学杂志,2010,30(9):738-740.
[22]张丽芝.酶协同微波辅助提取红枣多糖的工艺优化[J].食品与机械,2016,32(11):145-147.
[23]郑飞.蓝莓多糖对衰老小鼠运动耐力及抗疲劳效果研究[J].食品科学,2014,35(21):249-252.DOI:10.7506/spkx1002-6630-201421049.
[24]许牡丹,邹继伟,史芳.超声波辅助酶法提取木枣环磷酸腺苷的工艺条件优化[J].食品科技,2013,38(7):220-224.DOI:10.13684/j.cnki.spkj.2013.07.023.
[25]SUN Y,PAN D,GUO Y.Purification of chicken breast protein hydrolysate and analysis of its antioxidant activity[J].Food and Chemical Toxicology,2012,50:3397-3404.
[26]CHEN X C,BAI J X,CAO J M,et al.Medium optimization for the production of cyclic adenosine 3’,5’-monophosphate by Microbacterium sp.no.205 using response surface methodology[J].Bioresource Technology,2009,100(2):919-924.DOI:10.1016/j.biortech.2008.07.062.
[27]ZHAO C Y,GREENSTEIN J L,WINSLOW R L.Interaction between phosphodiesterases in the regulation of the cardiacβ-adrenergic pathway[J].Journal of Molecular and Cellular Cardiology,2015,88:29-38.DOI:10.1016/j.yjmcc.2015.09.011.
[28]LEE J,KIM H,HAN J,et al.Anti-fatigue effect of Myelophil in a chronic forced exercise mouse model[J].European Journal of Pharmacology,2015,764(5):100-108.DOI:10.1016/j.ejphar.2015.06.055.
[29]王郅媛,王友升,李丽萍.抗衰老研究新模型-酵母细胞活性氧代谢研究进展[J].食品科学,2012,33(7):354-358.
[30]杨婷,张冲,陈清轩.衰老机制研究进展[J].中国生物工程杂志,2005,25(3):6-11.
[31]陈艳珍.复合微量营养素对衰老雌性小鼠抗疲劳和抗氧化作用的研究[J].山东理工大学学报(自然科学版),2009,23(5):1-4.
[32]赵凡凡,周玉枝,高丽,等.D-半乳糖致衰老大鼠模型的研究进展[J].药学学报,2017,52(3):347-354.