动物性食品中磺胺类药物残留检测方法研究进展
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摘要
磺胺类药物是一类人工合成广谱抗菌药物,因为其疗效好且价格相对低廉,在动物养殖和疾病防治中应用较为广泛。但这类药物在使用后易以原型排出,且大剂量、长时间使用时会造成其在动物产品中的残留,对人的健康和环境造成危害。对动物性食品中磺胺类药物残留的检测是保证产品食用安全和减少环境污染的重要措施。磺胺类药物由于种类多、使用广泛、在不同动物性产品中的残留情况也不相同,在对其残留进行检测时,应选择合适方法对样品进行处理和检测。本文介绍了样品前处理的常用方法,如液液萃取等传统方法和固相萃取法、固相微萃取法以及基质固相分散法等的原理、优缺点等。对动物性食品中磺胺类药物的检测,应用较多的是高效液相色谱法、毛细管电泳法放射免疫法、免疫传感器发和酶联免疫法等,可使实现对产品中药物残留的定性和定量检测,但是也存在需要专门仪器、检测时间相对较长的问题;快速检测方法最常用的就是胶体金免疫层析方法,可以实现对中多种磺胺类药物的快速和定性的检测,是食品种抗菌药物检测技术研究的一个发展方向。通过对动物性食品中抗微生物残留进行初步筛查,确定为阳性的样品在进行实验室的定量检测,是保证产品安全、可操作的基本程序。研究高通量、敏感、快速、操作简便的动物性食品中磺胺类药物残留检测技术,也是一个重要方面。
Sulfonamides are a class of synthetic broad-spectrum antibacterial drugs, because of their good efficacy and relatively low price, these drugs are widely used in animal breeding and disease prevention. However, such drugs are easily excreted by the prototype after use, and high dosage and long-term use may cause residues in animal products, which may cause harm to human health and the environment. Therefore, detection of sulfonamide residues in animal derived foods is an important measure to ensure the safety of products and to reduce environmental pollution. Due to the wide variety of sulfonamides and their wide use, the residues in different animal derived products also vary. When testing the residues, the samples should be processed and tested by an appropriate method. This paper introduces the common methods of sample pretreatment, including the principles, advantages and disadvantages of traditional methods(liquid-liquid extraction), solid phase extraction, solid phase microextraction, and matrix solid phase dispersion. For detection of sulfonamides in animal derived foods, high-performance liquid chromatography, capillary electrophoresis, radioimmunoassay, immunosensor and enzyme-linked immunoassay may be used to qualitatively and quantitatively determine drug residues in products. However, there are also problems of requiring specialized instruments and relatively long detection time. The most commonly used method for rapid detection in this regard is colloidal gold immunochromatography, which can achieve rapid and qualitative detection of a variety of sulfonamides, which is a direction in developing antibacterial drug detection technology for foods. By performing preliminary screening of antimicrobial residues in animal derived foods, quantitative determination of samples that are positive in the laboratory is the basic procedure for ensuring product safety and easy operation. This is also an important aspect of research on the detection technology of sulfonamide residues in animal derived foods with high throughput, sensitivity, rapidity and easy operation.
Sulfonamides are a class of synthetic broad-spectrum antibacterial drugs, because of their good efficacy and relatively low price, these drugs are widely used in animal breeding and disease prevention. However, such drugs are easily excreted by the prototype after use, and high dosage and long-term use may cause residues in animal products, which may cause harm to human health and the environment. Therefore, detection of sulfonamide residues in animal derived foods is an important measure to ensure the safety of products and to reduce environmental pollution. Due to the wide variety of sulfonamides and their wide use, the residues in different animal derived products also vary. When testing the residues, the samples should be processed and tested by an appropriate method. This paper introduces the common methods of sample pretreatment, including the principles, advantages and disadvantages of traditional methods(liquid-liquid extraction), solid phase extraction, solid phase microextraction, and matrix solid phase dispersion. For detection of sulfonamides in animal derived foods, high-performance liquid chromatography, capillary electrophoresis, radioimmunoassay, immunosensor and enzyme-linked immunoassay may be used to qualitatively and quantitatively determine drug residues in products. However, there are also problems of requiring specialized instruments and relatively long detection time. The most commonly used method for rapid detection in this regard is colloidal gold immunochromatography, which can achieve rapid and qualitative detection of a variety of sulfonamides, which is a direction in developing antibacterial drug detection technology for foods. By performing preliminary screening of antimicrobial residues in animal derived foods, quantitative determination of samples that are positive in the laboratory is the basic procedure for ensuring product safety and easy operation. This is also an important aspect of research on the detection technology of sulfonamide residues in animal derived foods with high throughput, sensitivity, rapidity and easy operation.
引文
[1] 何世超,Jeyakkumar P,Rao A S,等.磺胺类药物化学研究新进展[J].中国科学(化学),2016,46(9):823-847.
[2] 于淼.养殖业中磺胺类药物残留的危害及现状[J].现代畜牧科,2015,(2):133.
[3] Blasco C,Pico Y,Torres C M.Progress in analysis of residual antibacterials in food[J].TrAC Trends in Analyt Chem,2007,26(9):895-913.
[4] 郝昀,李挥,孙汉文.加速溶剂萃取在动物源食品农兽药残留分析中的应用进展[J].河北大学学报(自然科学版),2012,32(4):434-448.
[5] 李晓晶,于鸿,杨蓉,等.超声辅助分散液液微萃取/超高效液相色谱-串联质谱法检测水中多种磺胺类抗生素残留[J].分析测试学报,2016,35(10):1255-1260.
[6] Cai Z X,Zhang Y,Pan H F,et al.Simultaneous determination of 24 sulfonamide residues in meat by ultra - performance liquid chromatography tandem mass spectrometry[J].J Chromatogr A,2008,1200:144-155.
[7] 刘长武,翟广书,买光熙,等.固相萃取技术的原理及进展[J].农业环境与发展,2003(1):42-44.
[8] Wang H,Xu Y,Song W T,et al.Automatic sample preparation of sulfonamide antibiotic residues in chicken breast muscle by using dynamic microwave-assisted extraction coupled with solid-phase extraction[J].J Sep Sci,2011,34(18):2489-2497.
[9] 王建凤,杜振霞,王颖,等.过滤型固相萃取-超高效液相色谱/质谱法快速测定牛奶中喹诺酮及磺胺类药物[J].分析试验室,2018,37(5):599-603.
[10] Belardi R G,Pawliszyn J.The application of chemically modified fused silica fibers in the extraction of organics from water matrix samples and their rapid transfer to capillary columns[J].Water Air Soil Poll,1989,24:179-191.
[11] 王浩天.芯片管内固相微萃取与高分辨质谱联用应用于磺胺类药物的快速检测[D].北京:北京工业大学,2017.
[12] 徐尉力,聂稳,张凯丽,等.基于离子液体的基质固相分散萃取结合高效液相色谱法测定肌肉组织中的氟喹诺酮类抗生素[J].食品科学,2017,38(16):210-215.
[13] Hou X L,Wu Y L,Yang T,et al.Multi-walled carbon nanotubes-dispersive solid-phase extraction combined with liquid chromatography-tandem mass spectrometry for the analysis of 18 sulfonamides in pork[J].J Chromatogr B,2013,929:107-115.
[14] Zhamg Y P,Xu X,Qi X,et al.Determination of sulfonamides in livers using matrix solid-phase dispersion extraction high-performance liquid chromatography[J].J Sep Sci,2012,35(1):45-52.
[15] Lu Y B,Shen Q,Dai Z Y,et al.Development of an on-line matrix solid-phase dispersion/fast liquid chromatography/tandem mass spectrometry system for the rapid and simultaneous determination of 13 sulfonamides in grass carp tissues[J].J Chromatogr A,2011,1218(7):929-937.
[16] 王重洋.基质固相分散—色谱法分析食品中磺胺类兽药残留、苏丹红染料及挥发性成分[D].吉林:吉林大学,2014.
[17] 王菊梅.加速溶剂提取-高效液相色谱法测定奶粉中磺胺类药物的含量[J].中国卫生检验杂志,2011,21(5):1103-1106.
[18] Li J S,Li X W,Yuan J K,et al.Determination of sulfonamides in swine meat by immunoaffinity chromatography[J].J Aoacinternat,2000,83(4):830-836.
[19] 袁中珍,张雷,唐柏彬,等.免疫亲和柱净化/高效液相色谱法同时测定动物源性食品中16种磺胺类药物残留[J].分析测试学报,2013,32(4):478-482.
[20] 樊惠民,余实,谭远方.液相色谱在食品检测方面的应用[J].食品安全质量检测学报,2011,2(3):174-177.
[21] 许旭,肖远灿,耿丹丹,等.在线柱后衍生-高效液相色谱-荧光检测法同时测定牛肉中16种磺胺类药物残留[J].色谱,2016,34(4):422-428.
[22] 黄冬梅,黄宣运,顾润润,等.柱后衍生高效液相色谱法测定虾中14种磺胺类药物残留量[J].色谱,2014,32(8):874-879.
[23] 滕爽,吕青骎,沈娟,等.超高效液相色谱法检测猪肉及其制品中的磺胺类及氟喹诺酮类兽药残留[J].食品与发酵工业,2018,44(7) :263-268.
[24] 王伟,张鹏飞,王丹慧,等.高效液相色谱法测定牛奶中十二种磺胺类药物残留[J].中国乳品工业,2015,43(5):54-56.
[25] 罗昭君.高效液相色谱-紫外串联荧光检测法测定猪肉中两类11种兽药残留的研究[J].广西兽药监察所,2018,34(5):247-249.
[26] 栾庆祥,黄鑫,孙鹃,等.超高效液相色谱法测定鸡肉中氯羟吡啶和磺胺类药物残留的方法研究[J].中国家禽,2018,40(11):33-36.
[27] 孙鑫泽,张德花.超高效液相色谱-串联质谱法测定畜禽肉中5种磺胺类药物残留[J].食品安全导刊,2018(18):64.
[28] 舒顺顺,米倩,李佳丽,等.液相色谱-串联质谱法检测猪肉中5种磺胺类药物残留[J].食品安全质量检测学报,2018,9(11):2836-2841.
[29] 周靖雯,吴友谊,殷斌.中空纤维膜液相微萃取-毛细管电泳法测定环境水样中的4种磺胺类药物[J].理化检验(化学分册),2018,54(6):627-633.
[30] Cheng Y J,Huang S H,Singco B,et al .Analyses of sulfonamide antibiotics in meat samples by on-line concentration capillary electrochromatography-mass spectrometry[J].J Chromatogr A,2011,1218:7640-7647.
[31] 宋予震,董青,邢广旭,等.免疫分析方法在磺胺类药物残留检测中的应用[J].河南农业科学,2017,46(10):8-12.
[32] Pastor-Navarro N,Gallego-Iglesias E,Maquieira A,et al.Development of a group-specific immunoassay for sulfon- amides:Application to bee honey analysis[J].Talanta,2007,71(2):923-933.
[33] 杜玉玲,常迪,吴国英,等.磺胺母核单克隆抗体的制备及其免疫学特性研究[J].中国食品学报,2013,13(12):139-145.
[34] 林品言,杜红梅.酶联免疫法快速检测水产品中磺胺类药物残留[J].当代水产,2016(10):94-95.
[35] 赵彦华,刘洪岩,丁正峰,等.胶体金免疫层析技术在水产品药物残留检测中的应用进展[J].水产养殖,2016,37(7):1-4.
[36] Han S J,Zhou T J,Yin B J,et al.A sensitive and semi-quantita-tive method for determination of multi-drug residues in animal body fluids using multiplex dipstick immunoassay[J].Anal Chim Acta,2016,927:64-71.
[37] Wang C,Li X M,Peng T,et al.Latex bead and colloidal gold applied in a multiplex immunochromatographic assay for high-throughput detection of three classes of antibiotic residues in milk[J].Food Control,2017,77:1-7.
[38] Chen Y N,Liu L Q,Xu L G,et al.Gold immunochromatographic sensor for the rapid detection of twenty-six sulfonamides in foods[J].J Nano Res-Sw,2017,10(8):2833-2844.
[2] 于淼.养殖业中磺胺类药物残留的危害及现状[J].现代畜牧科,2015,(2):133.
[3] Blasco C,Pico Y,Torres C M.Progress in analysis of residual antibacterials in food[J].TrAC Trends in Analyt Chem,2007,26(9):895-913.
[4] 郝昀,李挥,孙汉文.加速溶剂萃取在动物源食品农兽药残留分析中的应用进展[J].河北大学学报(自然科学版),2012,32(4):434-448.
[5] 李晓晶,于鸿,杨蓉,等.超声辅助分散液液微萃取/超高效液相色谱-串联质谱法检测水中多种磺胺类抗生素残留[J].分析测试学报,2016,35(10):1255-1260.
[6] Cai Z X,Zhang Y,Pan H F,et al.Simultaneous determination of 24 sulfonamide residues in meat by ultra - performance liquid chromatography tandem mass spectrometry[J].J Chromatogr A,2008,1200:144-155.
[7] 刘长武,翟广书,买光熙,等.固相萃取技术的原理及进展[J].农业环境与发展,2003(1):42-44.
[8] Wang H,Xu Y,Song W T,et al.Automatic sample preparation of sulfonamide antibiotic residues in chicken breast muscle by using dynamic microwave-assisted extraction coupled with solid-phase extraction[J].J Sep Sci,2011,34(18):2489-2497.
[9] 王建凤,杜振霞,王颖,等.过滤型固相萃取-超高效液相色谱/质谱法快速测定牛奶中喹诺酮及磺胺类药物[J].分析试验室,2018,37(5):599-603.
[10] Belardi R G,Pawliszyn J.The application of chemically modified fused silica fibers in the extraction of organics from water matrix samples and their rapid transfer to capillary columns[J].Water Air Soil Poll,1989,24:179-191.
[11] 王浩天.芯片管内固相微萃取与高分辨质谱联用应用于磺胺类药物的快速检测[D].北京:北京工业大学,2017.
[12] 徐尉力,聂稳,张凯丽,等.基于离子液体的基质固相分散萃取结合高效液相色谱法测定肌肉组织中的氟喹诺酮类抗生素[J].食品科学,2017,38(16):210-215.
[13] Hou X L,Wu Y L,Yang T,et al.Multi-walled carbon nanotubes-dispersive solid-phase extraction combined with liquid chromatography-tandem mass spectrometry for the analysis of 18 sulfonamides in pork[J].J Chromatogr B,2013,929:107-115.
[14] Zhamg Y P,Xu X,Qi X,et al.Determination of sulfonamides in livers using matrix solid-phase dispersion extraction high-performance liquid chromatography[J].J Sep Sci,2012,35(1):45-52.
[15] Lu Y B,Shen Q,Dai Z Y,et al.Development of an on-line matrix solid-phase dispersion/fast liquid chromatography/tandem mass spectrometry system for the rapid and simultaneous determination of 13 sulfonamides in grass carp tissues[J].J Chromatogr A,2011,1218(7):929-937.
[16] 王重洋.基质固相分散—色谱法分析食品中磺胺类兽药残留、苏丹红染料及挥发性成分[D].吉林:吉林大学,2014.
[17] 王菊梅.加速溶剂提取-高效液相色谱法测定奶粉中磺胺类药物的含量[J].中国卫生检验杂志,2011,21(5):1103-1106.
[18] Li J S,Li X W,Yuan J K,et al.Determination of sulfonamides in swine meat by immunoaffinity chromatography[J].J Aoacinternat,2000,83(4):830-836.
[19] 袁中珍,张雷,唐柏彬,等.免疫亲和柱净化/高效液相色谱法同时测定动物源性食品中16种磺胺类药物残留[J].分析测试学报,2013,32(4):478-482.
[20] 樊惠民,余实,谭远方.液相色谱在食品检测方面的应用[J].食品安全质量检测学报,2011,2(3):174-177.
[21] 许旭,肖远灿,耿丹丹,等.在线柱后衍生-高效液相色谱-荧光检测法同时测定牛肉中16种磺胺类药物残留[J].色谱,2016,34(4):422-428.
[22] 黄冬梅,黄宣运,顾润润,等.柱后衍生高效液相色谱法测定虾中14种磺胺类药物残留量[J].色谱,2014,32(8):874-879.
[23] 滕爽,吕青骎,沈娟,等.超高效液相色谱法检测猪肉及其制品中的磺胺类及氟喹诺酮类兽药残留[J].食品与发酵工业,2018,44(7) :263-268.
[24] 王伟,张鹏飞,王丹慧,等.高效液相色谱法测定牛奶中十二种磺胺类药物残留[J].中国乳品工业,2015,43(5):54-56.
[25] 罗昭君.高效液相色谱-紫外串联荧光检测法测定猪肉中两类11种兽药残留的研究[J].广西兽药监察所,2018,34(5):247-249.
[26] 栾庆祥,黄鑫,孙鹃,等.超高效液相色谱法测定鸡肉中氯羟吡啶和磺胺类药物残留的方法研究[J].中国家禽,2018,40(11):33-36.
[27] 孙鑫泽,张德花.超高效液相色谱-串联质谱法测定畜禽肉中5种磺胺类药物残留[J].食品安全导刊,2018(18):64.
[28] 舒顺顺,米倩,李佳丽,等.液相色谱-串联质谱法检测猪肉中5种磺胺类药物残留[J].食品安全质量检测学报,2018,9(11):2836-2841.
[29] 周靖雯,吴友谊,殷斌.中空纤维膜液相微萃取-毛细管电泳法测定环境水样中的4种磺胺类药物[J].理化检验(化学分册),2018,54(6):627-633.
[30] Cheng Y J,Huang S H,Singco B,et al .Analyses of sulfonamide antibiotics in meat samples by on-line concentration capillary electrochromatography-mass spectrometry[J].J Chromatogr A,2011,1218:7640-7647.
[31] 宋予震,董青,邢广旭,等.免疫分析方法在磺胺类药物残留检测中的应用[J].河南农业科学,2017,46(10):8-12.
[32] Pastor-Navarro N,Gallego-Iglesias E,Maquieira A,et al.Development of a group-specific immunoassay for sulfon- amides:Application to bee honey analysis[J].Talanta,2007,71(2):923-933.
[33] 杜玉玲,常迪,吴国英,等.磺胺母核单克隆抗体的制备及其免疫学特性研究[J].中国食品学报,2013,13(12):139-145.
[34] 林品言,杜红梅.酶联免疫法快速检测水产品中磺胺类药物残留[J].当代水产,2016(10):94-95.
[35] 赵彦华,刘洪岩,丁正峰,等.胶体金免疫层析技术在水产品药物残留检测中的应用进展[J].水产养殖,2016,37(7):1-4.
[36] Han S J,Zhou T J,Yin B J,et al.A sensitive and semi-quantita-tive method for determination of multi-drug residues in animal body fluids using multiplex dipstick immunoassay[J].Anal Chim Acta,2016,927:64-71.
[37] Wang C,Li X M,Peng T,et al.Latex bead and colloidal gold applied in a multiplex immunochromatographic assay for high-throughput detection of three classes of antibiotic residues in milk[J].Food Control,2017,77:1-7.
[38] Chen Y N,Liu L Q,Xu L G,et al.Gold immunochromatographic sensor for the rapid detection of twenty-six sulfonamides in foods[J].J Nano Res-Sw,2017,10(8):2833-2844.