LCMS-IT-TOF法分析黄芪赤风汤提取物的化学成分
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摘要
目的:分析鉴别黄芪赤风汤提取物化学成分。方法:采用高效液相色谱-离子阱-飞行时间质谱法(LCMS-IT-TOF)对黄芪赤风汤提取物化学成分进行分析,采用Venusil MP-C18色谱柱(4.6 mm×250 mm,5μm),乙腈(A)-水(B)为流动相进行梯度洗脱,电喷雾离子源为质谱离子源,正、负离子检测模式,获得不同化合物的多级质谱数据。结果:根据高分辨质谱数据信息,结合对照品质谱信息及相关文献,共鉴定推断出41个化合物。结论:LCMS-IT-TOF可全面、快速分析黄芪赤风汤提取物中的化学成分,为其质量控制提供参考。
Objective: To analyze chemical compositions in the extract of Huangqi Chifeng Decoction. Methods: The liquid chromatography-mass spectrometry-ion trap-time-of-flight( LCMS-ITTOF) method was applied to analyze the extract of Huangqi Chifeng Decoction. The chromatographic separation was performed on Venusil MP-C18 column( 4. 6 mm × 250 mm,5 μm) with gradient elution of acetonitrile( A)-water( B). Multistage mass spectrometry data of different compounds was obtained by using electrospray ionization source and monitoring in positive and negative ion modes. Results: Forty-one chemical constituents were identified based on the characteristic MS/MS fragment ions data with chemical standards and published literature. Conclusion: The method is rapid and comprehensive,which is useful for quality control of the extract of Huangqi Chifeng Decoction.
Objective: To analyze chemical compositions in the extract of Huangqi Chifeng Decoction. Methods: The liquid chromatography-mass spectrometry-ion trap-time-of-flight( LCMS-ITTOF) method was applied to analyze the extract of Huangqi Chifeng Decoction. The chromatographic separation was performed on Venusil MP-C18 column( 4. 6 mm × 250 mm,5 μm) with gradient elution of acetonitrile( A)-water( B). Multistage mass spectrometry data of different compounds was obtained by using electrospray ionization source and monitoring in positive and negative ion modes. Results: Forty-one chemical constituents were identified based on the characteristic MS/MS fragment ions data with chemical standards and published literature. Conclusion: The method is rapid and comprehensive,which is useful for quality control of the extract of Huangqi Chifeng Decoction.
引文
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[2]姚余有,周爱武,陈敏珠.黄芪总苷对兔关节软骨和滑膜作用的体外实验[J].中国新药杂志,2004,13(4):315-316.
[3]徐建华,徐胜前,帅宗文,等.黄芪总甙治疗类风湿关节炎临床研究[J].中国药物与临床,2002,2(3):157-160.
[4]刘超,王静,杨军.赤芍总甙活血化瘀作用的研究[J].中药材,2000,23(9):557-560.
[5]冀兰鑫,黄浩,李长志,等.赤芍药理作用的研究进展[J].药物评价研究,2010,33(3):233-236.
[6]李文,李丽,是元艳,等.防风有效部位的药理作用研究[J].中国实验方剂学杂志,2006,12(6):29-31.
[7]薛宝云,李文,李丽,等.防风色原酮甙类成分的药理活性研究[J].中国中药杂志,2000,25(5):297-299.
[8]Yulong Gu,Bin Liu,Yanyan Jiang,et al.Simultaneous determination of 5 major constituents in the effective fractions of Huangqichifeng Medicinal Broth,By UPLC-MS/MS[J].J Liq Chromatogr Relat Technol,2014,37:1404-1415.
[9]姜艳艳,朱颖超,刘斌.高效液相色谱法同时测定黄芪赤风胶囊中芍药苷、升麻素苷等9种成分的含量[J].中国药学杂志,2012,47(15):1246-1249.
[10]谷雨龙,刘斌,姜艳艳.高效液相色谱法测定黄芪赤风汤提取物中萜类和色酮类成分的含量[J].中国药学杂志,2017,10(3):293-296.
[11]宋兆辉,王保德,巴杭,等.新疆芍药化学成分的研究[J].中国中药杂志,2004,29(8):748-751.
[12]Kang J,Sun JH,Zhou L,et al.Characterization of compounds from the roots of Saposhnikovia divaricata by high-performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry[J].Rapid Commun Mass Spectrom,2008,22(12):1899-1911.
[13]李悦悦,王慧,陈俊,等.RRLC-TOF/MS鉴别防风血浆、尿液中成分及代谢产物[J].第二军医大学学报,2010,31(7):760-763.
[14]杨素珍,蒋建勤.新疆芍药的化学成分研究[J].中草药,2014,45(6):760-764.
[15]王彦志,冯卫生,石任兵,等.赤芍中的单萜类成分[J].中国药学杂志,2008,43(9):669-671.
[16]刘杰,陈琳,范彩荣,等.基于HPLC-DAD-Q-TOF-MS/MS的白芍和赤芍主要成分定性定量研究[J].中国中药杂志,2015,40(9):1762-1770.
[17]刘杰,许文,李煌,等.UPLC-MS/MS法同时测定白芍饮片中10种成分[J].药物分析杂志,2015,35(4):635-643.
[18]Xiao CN,Wu M,Chen YY,et al.Revealing Metabolomic Variations in Cortex Moutan from Different Root Parts using HPLC-MS method[J].Phytochem Anal,2015,26(1):86-93.
[19]Cao J,Qiao X,Ji S,et al.Insights into the neutral loss of 30 Da of Paeonia monoterpene glycosides in electrospray ionization tandem mass spectrometry and the rapid screening of monoterpene glycosides by LC/MS/MS[J].J Chin Pharm Sci,2016,25(1):37-45.
[20]Cao HX,Zhang AH,Zhang FM,et al.Ultra-performance liquid chromatography tandem mass spectrometry combined with automated Metabo Lynx analysis approach to screen the bioactive components and their metabolites in Wen-Xin-Formula[J].Biomed Chromatogr,2014,28(12):1774-1781.
[21]Tao Y,Su DD,Du YS,et al.Magnetic solid-phase extraction coupled with HPLC-Q-TOF-MS for rapid analysis of tyrosinase binders from San-Bai decoction by Box-Behnken statistical design[J].RSC Adv,2016,6(111):109730-109741.
[22]Dong HJ,Liu ZQ,Song FR,et al.Structural analysis of monoterpene glycosides extracted from Paeonia lactiflora Pall.using electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry and high-performance liquid chromatography/electrospray ionization tandem mass spectrometry[J].Rapid Commun Mass Spectrom,2007,21(19):3193-3199.
[23]陈琴华,李鹏,朱军.电喷雾离子阱质谱检测升麻素苷的正离子裂解途径[J].湖北医药学院学报,2012,31(3):201-203.
[24]Chen QH,Li P,Li B,et al.Characterization and quantification of prim-O-glucosylcimifugin in the roots of Saposhnikovia divaricata and its medicinal preparations by liquid chromatography-ion trap mass spectrometry[J].J Liq Chromatogr Relat Technol,2013,36(11):1589-1596.
[25]Jiang WY,KAN H,Li PD,et al.Screening and structural characterization of potentialα-glucosidase inhibitors from Radix Astragali flavonoids extract by ultrafiltration LC-DAD-ESI-MSn[J].Anal Methods,2015,7(1):123-128.
[26]Tan HP,Ling SK,Chuah CH.Characterisation of Galloylated Cyanogenic Glucosides and Hydrolysable Tannins from Leaves of Phyllagathis rotundifolia by LC-ESI-MS/MS[J].Phytochem Anal,2011,22(6):516-525.
[27]Wang YL,Ding CG,Du KH,et al.Identification of active compounds and their metabolites by high-performance liquid chromatography/electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry from Xiao-xu-ming decoction(XXMD)[J].Rapid Commun Mass Spectrom,2009,23(17):2724-2732.
[28]Jaiswal R,Jayasinghe L,Kuhnert N.Identification and characterization of proanthocyanidins of 16 members of the Rhododendron genus(Ericaceae)by tandem LC-MS[J].J Mass Spectrom,2012,47(4):502-515.
[29]姜艳艳,刘斌,石任兵,等.防风化学成分的分离与结构鉴定[J].药学学报,2007,42(5):505-510.
[30]练杭芸,徐王彦君,梁乾德,等.UPLC-QTOF MS法比较赤芍、白芍煎液化学成分差异[J].质谱学报,2014,35(3):269-278.
[31]李丽,刘质净,桂语歌,等.防风中的色原酮化合物的高效液相色谱与电喷雾质谱联用研究[J].时珍国医国药,2010,21(6):1310-1311.
[32]何忠梅,孙佳明,张辉,等.固相萃取-高效液相色谱-电喷雾串联质谱法分析芍药和酒芍药的水溶性化学成分[J].分析化学,2009,37(8):1201-1205.
[33]孙冬梅,董玉娟,胥爱丽,等.葛根枳椇软胶囊中黄酮类化合物的UPLC/Q-TOF-MS快速分析[J].中草药,2015,46(7):970-976.
[34]Zhang LJ,Liu HK,Hsiao PC,et al.New Isoflavonoid Glycosides and Related Constituents from AstragaliRadix(Astragalus membranaceus)and Their Inhibitory Activity on Nitric Oxide Production[J].J Agric Food Chem,2011,59(4):1131-1137.
[35]李忠红,倪坤仪,杜冠华.高效液相色谱-质谱法鉴定中药复方小续命汤有效成分组中醇溶性成分[J].分析化学,2007,35(2):233-239.
[36]Liu MH,Tong X,Wang JX,et al.Rapid separation and identification of multiple constituents in traditional Chinese medicine formula Shenqi Fuzheng Injection by ultra-fast liquid chromatography combined with quadrupole-time-of-flight mass spectrometry[J].J Pharm Biomed Anal,2013,74:141-155.
[37]Krasteva I,Platikanov S,Nikolov S,et al.Flavonoids from Astragalus hamosus[J].Nat Prod Res,2007,21(5):392-395.
[38]卞云云,管佳,毕志明,等.蒙古黄芪的化学成分研究[J].中国药学杂志,2006,41(16):1217-1221.
[39]康利平,赵阳,余河水,等.采用UPLC-Q-TOF/MSE鉴别芪苈强心胶囊有效部位中的化学成分[J].药学学报,2011,46(10):1231-1236.
[40]覃红萍,鲁静,林瑞超,等.黄芪中异黄酮类成分的研究[J].药学分析杂志,2009,29(5):746-751.
[41]张靖,丘小慧,徐文,等.基于液质联用技术的复方三芪口服液化学成分特征图谱研究[J].中华中医药杂志,2013,28(5):1572-1576.
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