HPLC法同时测定绿咖啡豆中绿原酸及咖啡碱含量
|
摘要
建立一种高效液相色谱法(HPLC),可同时定量绿咖啡豆中绿原酸和咖啡碱的含量,并分析不同成熟度绿咖啡豆中绿原酸和咖啡碱含量变化。采用Agilent ZORBAX ODS-C18色谱柱,以0.5%乙酸水-乙腈为流动相,梯度洗脱,检测波长274 nm,流速1.0 m L/min,进样量10μL,柱温35℃。结果显示,绿原酸和咖啡碱的浓度线性范围分别为5.0 mg/L~100.0 mg/L(r~2=0.999 9)和5.1 mg/L~101.6 mg/L(r~2=0.999 9),检出限分别为0.25 mg/L和0.05 mg/L,相对标准偏差分别为1.56%和1.30%,回收率范围分别为98.86%~101.11%(RSD=1.02%)和98.70%~101.19%(RSD=0.83%)。
A high performance liquid chromatography method was developed for simultaneous determination of chlorogenic acid and caffeine in raw coffee beans. The chlorogenic acid and caffeine contents in raw coffee beans of different maturity were analyzed. The separation was performed on a Agilent ZORBAX ODS-C18 column with a gradient elution system. The mobile phase consisted of 0.5 % acetic acid solution and acetonitrile. The detection UV wavelength was at 274 nm. The flow rate was 1.0 mL/min. The injection volume was 10 μL. The column temperature was 35 ℃. The results showed that the linear ranges of chlorogenic acid and caffeine were 5.0 mg/L-100.0 mg/L(r~2=0.999 9)and 5.1 mg/L-101.6 mg/L(r~2=0.999 9),respectively. The limits of detection(LOD)were 0.25 mg/L and 0.05 mg/L,respectively. The RSDs of the method were 1.56 % and 1.30 %. The spiked recoveries of the two analytes were 98.86 %-101.11 %(RSD=1.02 %)and 98.70 %-101.19 %(RSD=0.83 %),respectively.
A high performance liquid chromatography method was developed for simultaneous determination of chlorogenic acid and caffeine in raw coffee beans. The chlorogenic acid and caffeine contents in raw coffee beans of different maturity were analyzed. The separation was performed on a Agilent ZORBAX ODS-C18 column with a gradient elution system. The mobile phase consisted of 0.5 % acetic acid solution and acetonitrile. The detection UV wavelength was at 274 nm. The flow rate was 1.0 mL/min. The injection volume was 10 μL. The column temperature was 35 ℃. The results showed that the linear ranges of chlorogenic acid and caffeine were 5.0 mg/L-100.0 mg/L(r~2=0.999 9)and 5.1 mg/L-101.6 mg/L(r~2=0.999 9),respectively. The limits of detection(LOD)were 0.25 mg/L and 0.05 mg/L,respectively. The RSDs of the method were 1.56 % and 1.30 %. The spiked recoveries of the two analytes were 98.86 %-101.11 %(RSD=1.02 %)and 98.70 %-101.19 %(RSD=0.83 %),respectively.
引文
[1]王飞,杨晨晖,陈榕.超声波辅助提取咖啡豆中的绿原酸[J].高校实验室工作研究,2015(4):44-45
[2]Azuma K,Ippoushi K,Nakayama M,et al.Absorption of chlorogenic acid and caffeic acid in rats after oral administration[J].J Agric Food Chem,2000,48(11):5496-5500
[3]Ky C L,Louarn J,Dussert S.Caffeine,trigonelline,chlorogenic acids and sucrose diversity in wild Coffea arabica L.and C.canephora P.accessions[J].Food chemistry,2001,75(2):223-230
[4]龙文静.咖啡豆中咖啡因与绿原酸的研究进展[J].广西轻工业,2010,26(1):1-2,112
[5]王立志,胡晓梅,付其胜,等.绿咖啡豆中总绿原酸的纯化工艺研究[J].中国食品添加剂,2014(7):123-128
[6]Farah A,Monteiro M C,Calado V.Correlation between cup quality and chemical attributes of Brazilian coffee[J].Food Chemistry,2006,98(2):373-380
[7]PsotováJ,Chlop cíková S,MiketováP.Chemoprotective effect of plant phenolics against anthracycline-induced toxicity on rat cardiomyocytes.Part III.Apigenin,baicalelin,kaempherol,luteolin and quercetin[J].Phytotherapy Research,2004,18(7):516-521
[8]Mcdougall B,King P J,Wu B W.Dicaffeoylquinic and dicaffeoyltartaric acids are selective inhibitors of human immunodeficiency virus type 1 integrase[J].Antimicrobial Agents and Chemotherapy,1998,42(1):140-146
[9]Shimizu M,Yoshimi N,Yamada Y.Suppressive effects of chlorogenic acid on N-methyl-N-nitrosourea-induced glandular stomach carcinogenesis in male F344 rats[J].The Journal of toxicological sciences,1999,24(5):433-439
[10]Jiang Y,Kusama K,Satoh K.Induction of cytotoxicity by chlorogenic acid in human oral tumor cell lines[J].Phytomedicine,2000,7(6):483-491
[11]Andrade Cetto A,Wiedenfeld H,Revilla M C,et al.Hypoglycemic effect of Equisetum myriochaetum aerial parts on streptozotocin diabetic rats[J].J Ethnopharmacol,2000,72(1-2):129-33
[12]Vinson J A,Burnham B R,Nagendran M V.Randomized,doubleblind,placebo-controlled,linear dose,crossover study to evaluate the efficacy and safety of a green coffee bean extract in overweight subjects[J].Diabetes Metab Syndr Obes,2012,5:21-27
[13]黄雅志.喝咖啡能免除得胆结石的危险[J].云南热作科技,2000(1):23
[14]龙文静,张盛,袁玲,等.反相高效液相色谱法同时测定咖啡豆中的6种酚酸类化合物[J].色谱,2011,29(5):439-442
[15]李春丽,贺明鑫.反相液相色谱法测定咖啡豆中咖啡碱含量[J].云南热作科技,2000(3):10-12
[16]贾桂燕,鲁巍巍,郑毅男.咖啡中咖啡因和绿原酸的含量测定[J].黑龙江八一农垦大学学报,2008,20(3):64-67
[17]袁凤琴,王佳,郭建敏,等.高效液相色谱法检测奶茶粉中咖啡因的含量[J].中国食品工业,2012(6):68-69
[18]杨清山,蔡荣华,徐猛,等.UPLC法测定咖啡豆中绿原酸和咖啡因的含量[J].食品研究与开发,2015,36(18):138-140
[19]蔡荣华,张晓芳,杜精精,等.超高效液相色谱-紫外检测法同时测定绿咖啡豆提取物中的绿原酸和咖啡因含量[J].中国食品添加剂,2014(2):225-228
[2]Azuma K,Ippoushi K,Nakayama M,et al.Absorption of chlorogenic acid and caffeic acid in rats after oral administration[J].J Agric Food Chem,2000,48(11):5496-5500
[3]Ky C L,Louarn J,Dussert S.Caffeine,trigonelline,chlorogenic acids and sucrose diversity in wild Coffea arabica L.and C.canephora P.accessions[J].Food chemistry,2001,75(2):223-230
[4]龙文静.咖啡豆中咖啡因与绿原酸的研究进展[J].广西轻工业,2010,26(1):1-2,112
[5]王立志,胡晓梅,付其胜,等.绿咖啡豆中总绿原酸的纯化工艺研究[J].中国食品添加剂,2014(7):123-128
[6]Farah A,Monteiro M C,Calado V.Correlation between cup quality and chemical attributes of Brazilian coffee[J].Food Chemistry,2006,98(2):373-380
[7]PsotováJ,Chlop cíková S,MiketováP.Chemoprotective effect of plant phenolics against anthracycline-induced toxicity on rat cardiomyocytes.Part III.Apigenin,baicalelin,kaempherol,luteolin and quercetin[J].Phytotherapy Research,2004,18(7):516-521
[8]Mcdougall B,King P J,Wu B W.Dicaffeoylquinic and dicaffeoyltartaric acids are selective inhibitors of human immunodeficiency virus type 1 integrase[J].Antimicrobial Agents and Chemotherapy,1998,42(1):140-146
[9]Shimizu M,Yoshimi N,Yamada Y.Suppressive effects of chlorogenic acid on N-methyl-N-nitrosourea-induced glandular stomach carcinogenesis in male F344 rats[J].The Journal of toxicological sciences,1999,24(5):433-439
[10]Jiang Y,Kusama K,Satoh K.Induction of cytotoxicity by chlorogenic acid in human oral tumor cell lines[J].Phytomedicine,2000,7(6):483-491
[11]Andrade Cetto A,Wiedenfeld H,Revilla M C,et al.Hypoglycemic effect of Equisetum myriochaetum aerial parts on streptozotocin diabetic rats[J].J Ethnopharmacol,2000,72(1-2):129-33
[12]Vinson J A,Burnham B R,Nagendran M V.Randomized,doubleblind,placebo-controlled,linear dose,crossover study to evaluate the efficacy and safety of a green coffee bean extract in overweight subjects[J].Diabetes Metab Syndr Obes,2012,5:21-27
[13]黄雅志.喝咖啡能免除得胆结石的危险[J].云南热作科技,2000(1):23
[14]龙文静,张盛,袁玲,等.反相高效液相色谱法同时测定咖啡豆中的6种酚酸类化合物[J].色谱,2011,29(5):439-442
[15]李春丽,贺明鑫.反相液相色谱法测定咖啡豆中咖啡碱含量[J].云南热作科技,2000(3):10-12
[16]贾桂燕,鲁巍巍,郑毅男.咖啡中咖啡因和绿原酸的含量测定[J].黑龙江八一农垦大学学报,2008,20(3):64-67
[17]袁凤琴,王佳,郭建敏,等.高效液相色谱法检测奶茶粉中咖啡因的含量[J].中国食品工业,2012(6):68-69
[18]杨清山,蔡荣华,徐猛,等.UPLC法测定咖啡豆中绿原酸和咖啡因的含量[J].食品研究与开发,2015,36(18):138-140
[19]蔡荣华,张晓芳,杜精精,等.超高效液相色谱-紫外检测法同时测定绿咖啡豆提取物中的绿原酸和咖啡因含量[J].中国食品添加剂,2014(2):225-228