黄鳝醛酮还原酶对大肠杆菌非生物胁迫耐受性的影响
|
摘要
醛酮还原酶是一类依赖NAD(P) H的氧化还原酶,在生物体内参与了糖代谢、酮代谢和物质运输等多种生理过程。为了解黄鳝醛酮还原酶在细胞逆境胁迫中的保护作用,采用液体培养和斑点展示法,比较含黄鳝醛酮还原酶Eakr的重组菌株和阴性对照菌株在Na Cl、Cu2+和H2O2等非生物胁迫下的存活率和生长活力。结果表明,含重组醛酮还原酶Eakr的大肠杆菌在高渗透压、重金属和氧化胁迫下的生存率显著高于阴性对照,其生存活力也大大增强。提示黄鳝醛酮还原酶Eakr可能参与了细胞活性氧代谢和物质运输等多种生理过程。
引文
[1]Ellis E M. Microbial aldo-keto reductases[J]. FEMS Microbiology Letters,2002,216(2):123-131.
[2]Penning T M. The aldo-keto reductases(AKRs):Overview[J].Chemico-Biological Interactions,2015,234:236-246.
[3]Lyon R C,Li D,Mcgarvie G,et al. Aldo-keto reductases mediate constitutive and inducible protection against aldehyde toxicity in human neuroblastoma SH-SY5Y cells[J]. Neurochemistry International,2013,62(1):113-121.
[4]Li D,Ferrari M,Ellis E M. Human aldo-keto reductase AKR7A2protects against the cytotoxicity and mutagenicity of reactive aldehydes and lowers intracellular reactive oxygen species in hamster V79-4cells[J]. Chemico-Biological Interactions,2012,195(1):25-34.
[5]Li D,Ellis E M. Aldo-keto reductase 7A5(AKR7A5)attenuates oxidative stress and reactive aldehyde toxicity in V79-4 cells[J].Toxicology in Vitro,2014,28(4):707-714.
[6]Zhang W,Li H,Yang Y,et al. Knockdown or inhibition of aldo-keto reductase 1B10 inhibits pancreatic carcinoma growth via modulating Kras-E-cadherin pathway[J]. Cancer Letters,2014,355(2):273-280.
[7]Ruiz F X,Moro A,Gallego O,et al. Human and rodent aldo-keto reductases from the AKR1B subfamily and their specificity with retinaldehyde[J]. Chemico-Biological Interactions,2011,191(1/2/3):199-205.
[8]Weng J,Cao Y,Moss N,et al. Modulation of voltage-dependent Shaker family potassium channels by an aldo-keto reductase[J].Journal of Biological Chemistry,2006,281(22):15194-15200.
[9]Kumar D,Singh P,Yusuf M A,et al. The Xerophyta viscosa aldose reductase(ALDRXV4)confers enhanced drought and salinity tolerance to transgenic tobacco plants by scavenging methylglyoxal and reducing the membrane damage[J]. Molecular Biotechnology,2013,54(2):292-303.
[10]va C,Solti,Oszvald M,et al. Improved reactive aldehyde,salt and cadmium tolerance of transgenic barley due to the expression of aldo-keto reductase genes[J]. Acta Physiologiae Plantarum,2016,38(4):1-10.
[11]代海艳,江翱,李伟.黄鳝醛酮还原酶的羰基解毒作用初探[J].江苏农业科学,2017,45(1):150-152.
[12]Jez J M,Penning T M. The aldo-keto reductase(AKR)superfamily:an update[J]. Chemico-Biological Interactions,2001,130-132:499-525.
[13]Barski O A,Tipparaju S M,Bhatnagar A. The aldo-keto reductase superfamily and its role in drug metabolism and detoxification[J].Drug Metabolism Reviews,2008,40(4):553-624.
[14]Mindnich R D,Penning T M. Aldo-keto reductase(AKR)superfamily:genomics and annotation[J]. Human Genomics,2009,3(4):362-370.
[15]Hintzpeter J,Martin H J,Maser E. Reduction of lipid peroxidation products and advanced glycation end-product precursors by cyanobacterial aldo-keto reductase AKR3G1-a founding member of the AKR3G subfamily[J]. FASEB Journal,2015,29(1):263-273.
[16]va C,Tóth G,Oszvald,et al. Overproduction of an Arabidopsis aldo-keto reductase increases barley tolerance to oxidative and cadmium stress by an in vivo reactive aldehyde detoxification[J].Plant Growth Regulation,2014,74(1):55-63.
[17]Agrawal C,Sen S,Yadav S,et al. A novel Aldo-Keto reductase(AKR17A1)of Anabaena sp. PCC 7120 degrades the rice field herbicide butachlor and confers tolerance to abiotic stresses in E.coli[J]. PLo S One,2015,10(9):e0137744.
[2]Penning T M. The aldo-keto reductases(AKRs):Overview[J].Chemico-Biological Interactions,2015,234:236-246.
[3]Lyon R C,Li D,Mcgarvie G,et al. Aldo-keto reductases mediate constitutive and inducible protection against aldehyde toxicity in human neuroblastoma SH-SY5Y cells[J]. Neurochemistry International,2013,62(1):113-121.
[4]Li D,Ferrari M,Ellis E M. Human aldo-keto reductase AKR7A2protects against the cytotoxicity and mutagenicity of reactive aldehydes and lowers intracellular reactive oxygen species in hamster V79-4cells[J]. Chemico-Biological Interactions,2012,195(1):25-34.
[5]Li D,Ellis E M. Aldo-keto reductase 7A5(AKR7A5)attenuates oxidative stress and reactive aldehyde toxicity in V79-4 cells[J].Toxicology in Vitro,2014,28(4):707-714.
[6]Zhang W,Li H,Yang Y,et al. Knockdown or inhibition of aldo-keto reductase 1B10 inhibits pancreatic carcinoma growth via modulating Kras-E-cadherin pathway[J]. Cancer Letters,2014,355(2):273-280.
[7]Ruiz F X,Moro A,Gallego O,et al. Human and rodent aldo-keto reductases from the AKR1B subfamily and their specificity with retinaldehyde[J]. Chemico-Biological Interactions,2011,191(1/2/3):199-205.
[8]Weng J,Cao Y,Moss N,et al. Modulation of voltage-dependent Shaker family potassium channels by an aldo-keto reductase[J].Journal of Biological Chemistry,2006,281(22):15194-15200.
[9]Kumar D,Singh P,Yusuf M A,et al. The Xerophyta viscosa aldose reductase(ALDRXV4)confers enhanced drought and salinity tolerance to transgenic tobacco plants by scavenging methylglyoxal and reducing the membrane damage[J]. Molecular Biotechnology,2013,54(2):292-303.
[10]va C,Solti,Oszvald M,et al. Improved reactive aldehyde,salt and cadmium tolerance of transgenic barley due to the expression of aldo-keto reductase genes[J]. Acta Physiologiae Plantarum,2016,38(4):1-10.
[11]代海艳,江翱,李伟.黄鳝醛酮还原酶的羰基解毒作用初探[J].江苏农业科学,2017,45(1):150-152.
[12]Jez J M,Penning T M. The aldo-keto reductase(AKR)superfamily:an update[J]. Chemico-Biological Interactions,2001,130-132:499-525.
[13]Barski O A,Tipparaju S M,Bhatnagar A. The aldo-keto reductase superfamily and its role in drug metabolism and detoxification[J].Drug Metabolism Reviews,2008,40(4):553-624.
[14]Mindnich R D,Penning T M. Aldo-keto reductase(AKR)superfamily:genomics and annotation[J]. Human Genomics,2009,3(4):362-370.
[15]Hintzpeter J,Martin H J,Maser E. Reduction of lipid peroxidation products and advanced glycation end-product precursors by cyanobacterial aldo-keto reductase AKR3G1-a founding member of the AKR3G subfamily[J]. FASEB Journal,2015,29(1):263-273.
[16]va C,Tóth G,Oszvald,et al. Overproduction of an Arabidopsis aldo-keto reductase increases barley tolerance to oxidative and cadmium stress by an in vivo reactive aldehyde detoxification[J].Plant Growth Regulation,2014,74(1):55-63.
[17]Agrawal C,Sen S,Yadav S,et al. A novel Aldo-Keto reductase(AKR17A1)of Anabaena sp. PCC 7120 degrades the rice field herbicide butachlor and confers tolerance to abiotic stresses in E.coli[J]. PLo S One,2015,10(9):e0137744.