蓝莓叶不同极性部位生物活性及化学成分研究
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摘要
目的研究蓝莓叶不同极性部位的生物活性及化学成分。方法蓝莓叶粗提物用不同有机溶剂萃取得到不同极性部位,再利用多种色谱法进行分离纯化,根据理化性质和波谱数据鉴定化合物的结构;以清除1,1-二苯基-2-三硝基苯肼自由基(DPPH·)、抑制α-葡萄糖苷酶和胰脂肪酶为指标,研究各极性部位和部分化合物的生物活性。结果正丁醇相(BF)具有最高的DPPH·清除和α-葡糖苷酶抑制活性,乙酸乙酯相(EAF)具有最高的胰脂肪酶抑制活性。从EAF中分离得到β-谷甾醇(1),槲皮素-3-O-α-L-呋喃阿拉伯糖苷(2),槲皮素(3),槲皮素-3-O-β-D-吡喃葡糖苷(4)和1-O-咖啡酰基奎宁酸(5)。从BF中分离得到槲皮素-3-O-α-L-阿拉伯糖(6)和槲皮素-3-O-β-D-葡糖醛酸苷(7)。化合物3和5有极好的清除DPPH·和胰脂肪酶抑制活性,化合物1和3有较好的α-葡萄糖苷酶抑制活性。结论蓝莓叶不同极性部位及化合物有较好的抗氧化、α-葡糖苷酶和胰脂肪酶抑制活性,化合物1,2,5,6为首次从蓝莓叶中分离得到。
OBJECTIVE To study the bioactivity and chemical constituents of different polar parts from blueberry leaves.METHODS Blueberry leaves were extracted by ethanol and then the extract was sequentially partitioned into five fractions. Silicagel and Sephadex LH-20 chromatographic methods were applied to isolate and purify compounds. Their structures were elucidated by physiochemical properties and spectral analysis. The DPPH· radical scavenging activity,α-glycosidase and pancreatic lipase inhibition activity of different polar parts and partial compounds were determined. RESULTS The n-butyl alcohol fraction( BF) showed the highest DPPH· radical scavenging activity and α-glycosidase inhibition activity. The ethyl acetate fraction( EAF) showed the strongest pancreatic lipase inhibition activity. A total of five compounds were isolated from the EAF,and their structures were identified as β-sitosterol( 1),quercetin-3-O-α-L-arabinofuranoside( 2),quercetin( 3),quercetin-3-O-β-D-glucopyranoside( 4) and 1-O-caffeoylquinic acid( 5). A total of two compounds were isolated from the BF,and their structures were identified as quercetin-3-O-α-L-arabinoside( 6) and quercetin-3-O-β-D-glucuronide( 7). The results showed that compounds 3 and 5 had very good DPPH· radical scavenging and pancreatic lipase inhibitory activity,and compounds 1 and 3 had good α-glucosidase inhibitory activity. CONCLUSION The different polar parts and compounds of blueberry leaves show strong DPPH· radical scavenging activity,α-glycosidase and pancreatic lipase inhibition activity. Compounds 1,2,5 and 6 are isolated from blueberry leaves for the first time.
OBJECTIVE To study the bioactivity and chemical constituents of different polar parts from blueberry leaves.METHODS Blueberry leaves were extracted by ethanol and then the extract was sequentially partitioned into five fractions. Silicagel and Sephadex LH-20 chromatographic methods were applied to isolate and purify compounds. Their structures were elucidated by physiochemical properties and spectral analysis. The DPPH· radical scavenging activity,α-glycosidase and pancreatic lipase inhibition activity of different polar parts and partial compounds were determined. RESULTS The n-butyl alcohol fraction( BF) showed the highest DPPH· radical scavenging activity and α-glycosidase inhibition activity. The ethyl acetate fraction( EAF) showed the strongest pancreatic lipase inhibition activity. A total of five compounds were isolated from the EAF,and their structures were identified as β-sitosterol( 1),quercetin-3-O-α-L-arabinofuranoside( 2),quercetin( 3),quercetin-3-O-β-D-glucopyranoside( 4) and 1-O-caffeoylquinic acid( 5). A total of two compounds were isolated from the BF,and their structures were identified as quercetin-3-O-α-L-arabinoside( 6) and quercetin-3-O-β-D-glucuronide( 7). The results showed that compounds 3 and 5 had very good DPPH· radical scavenging and pancreatic lipase inhibitory activity,and compounds 1 and 3 had good α-glucosidase inhibitory activity. CONCLUSION The different polar parts and compounds of blueberry leaves show strong DPPH· radical scavenging activity,α-glycosidase and pancreatic lipase inhibition activity. Compounds 1,2,5 and 6 are isolated from blueberry leaves for the first time.
引文
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[2] WANG L J,WU J,WANG H X,et al. Composition of phenolic compounds and antioxidant activity in the leaves of blueberry cultivars[J]. J Funct Foods,2015,16:295-304.
[3] LI Y C,LI B X,GENG L J. Hypolipidemic and antioxidant effects of total flavonoids from blueberry leaves[J]. Eur Food Res Technol,2011,233(6):897-903.
[4] WATSON E M. Some observations on the effect of blueberry leaf extract in diabetes mellitus[J]. Can Med Assoc J,1928,19(2):166-171.
[5] CHUNG S I,SU J N,XU M,et al. Aged ginseng(Panax ginseng Meyer)reduces blood glucose levels and improves lipid metabolism in high fat diet-fed mice[J]. Food Sci Biotechnol,2016,25(1):267-273.
[6] XU P,WU J,ZHANG Y,et al. Physicochemical characterization of puerh tea polysaccharides and their antioxidant andα-glycosidase inhibition[J]. J Func Foods,2014,6(1):545-554.
[7] BIRARI R B,BHUTANI K K. Pancreatic lipase inhibitors from natural sources:unexplored potential[J]. Drug Discov Today,2007,12(19):879-889.
[8] HANG T. Chemical constituents and quality-evaluation of Fructus Xanthii[D]. Shanghai:The Second Military Medical University,2006.
[9] YAN J,YAN Y M,WEI Y F,et al. Chemical constituents of acrial part of Bupleunum malconense[J]. Chin Tradit Herb Drugs(中草药),2017,48(7):1282-1285.
[10] ZHANG L,JIN Y Y,TIAN J K. Studies on chemical constituents of Hypericum japonicum[J]. Chin Pharm J(中国药学杂志),2007,42(5):341-344.
[11] MENG F T,TAN C Y,MAKHABEL B,et al. Study on chemical constituents of Echinops integrifolius[J]. Chin Pharm J(中国药学杂志),2014,49(7):539-542.
[12] XU X F,LI H J,LI P,et al. Chemical constituents in bud of Lonicera macranthoides[J]. Chin J Nat Med(中国天然药物),2006,4(1):45-48.
[13] ZHANG L,LI B G,FU H W,et al. Studies on the flavonoid glycosides from the leaves of Vaccinium bracteatum Thunb[J].Chin Pharm J(中国药学杂志),2009,44(23):1773-1776.