UPLC在中药及其复方分析中的应用和研究进展
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摘要
超高效液相色谱法(UPLC)是在高效液相色谱法(HPLC)的基础上发展而来的一种新兴的液相色谱技术,具有超高效、超高分离度、超高灵敏度等特点,已被广泛应用于中药成分分析、代谢组学研究、指纹图谱建立、与质谱等检测器联用测定未知化合物成分等方面。笔者通过查阅近5年的文献资料,对UPLC在中药领域的应用进行全面综述,并总结其相对运用优势和发展前景,以期为药学工作者能更好地运用UPLC技术提供科学指导和依据。
Ultra-high performance liquid chromatography( UPLC) is a new liquid chromatography technology,which developed on the basis of high performance liquid chromatography( HPLC). It has the characteristics of super high efficiency,high separation degree and high sensitivity,and has been widely used in the analysis of components in traditional Chinese medicine, the studies on metabolomics, establishment of fingerprint chromatogram, determination of unknown compounds combined with mass spectrometry and other detectors,etc. In this paper,the authors reviewed the application of UPLC in the field of traditional Chinese medicine through a review of the literature in the past 5 years, and summed up its relative advantages and development prospects,in order to provide scientific guidance and basis for pharmaceutical workers to make better use of UPLC technology.
Ultra-high performance liquid chromatography( UPLC) is a new liquid chromatography technology,which developed on the basis of high performance liquid chromatography( HPLC). It has the characteristics of super high efficiency,high separation degree and high sensitivity,and has been widely used in the analysis of components in traditional Chinese medicine, the studies on metabolomics, establishment of fingerprint chromatogram, determination of unknown compounds combined with mass spectrometry and other detectors,etc. In this paper,the authors reviewed the application of UPLC in the field of traditional Chinese medicine through a review of the literature in the past 5 years, and summed up its relative advantages and development prospects,in order to provide scientific guidance and basis for pharmaceutical workers to make better use of UPLC technology.
引文
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[2]盈竞男,韩新民.超高效液相色谱在中药及其复方研究中的应用进展[J].江苏中医药,2015,47(4):81-84.
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[4]戴朝政.超高效液相色谱塔板高度方程的推导[J].色谱,2015,33(5):535-540.
[5]刘丹,齐双辉,马海英. UPLC在中药组分分析及代谢组学中的应用进展[J].中国药房,2016,27(7):973-975.
[6]单玲玲,靳光乾,刘善新. UPLC、RRLC及UFLC在中药质量控制中的研究进展[J].中华中医药杂志,2011,26(10):2340-2344.
[7] Fayed A S,Hegazy M A,Wahab N S. Chromatographic analysis of a multicomponent mixture of B1,B6,B12,benfotiamine,and diclofenac; partⅠ:HPLC and UPLC methods for the simultaneous quantification of these five components in tablets and capsules[J]. J AOAC Int,2016,99(6):1513-1521.
[8]邓少东,王莲婧,林励,等.化橘红黄酮类成分UPLC与HPLC指纹图谱的比较研究[J].中草药,2013,44(9):1195-1198.
[9] Durand-Hulak M,Dugrand A,Duval T,et al. Mapping the genetic and tissular diversity of 64 phenolic compounds in Citrus species using a UPLC-MS approach[J]. Ann Bot,2015,115(5):861-877.
[10] Miranda T A,Silva P H,Pianetti G A,et al.Simultaneous quantitation of chloroquine and primaquine by UPLC-DAD and comparison with a HPLC-DAD method[J]. Malar J,2015,doi:10. 1186/s12936-015-0570-1.
[11] ZHAO M,LI G,QIU F,et al. Development and validation of a simple and rapid UPLC-MS assay for valproic acid and its comparison with immunoassay and HPLC methods[J]. Ther Drug Monit,2016,38(2):246-252.
[12] ZHAO X,WANG B,XIE K,et al. Development and comparison of HPLC-MS/MS and UPLC-MS/MS methods for determining eight coccidiostats in beef[J]. J Chromatogr B Analyt Technol Biomed Life Sci,2018,doi:10. 1016/j. jchromb. 2018. 04. 044.
[13]贾博,贺玉林,宋扬.超高效液相色谱在药物分析中的应用研究[J].化工管理,2017(20):182.
[14]朱渊,王俊虎. UPLC-MS法快速测定川楝子中川楝素的含量[J].亚太传统药,2018,14(4):54-56.
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