江苏某牛场生牛乳中肺炎克雷伯菌和鲍曼不动杆菌的分离鉴定、耐药性和毒力分析
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摘要
肺炎克雷伯菌和鲍曼不动杆菌均是重要的条件致病菌,其具备的多重耐药性是造成医院内感染并难以治愈的主要原因。本试验从江苏某奶牛场隐性乳房炎奶牛的生牛乳样品中分离到3株肺炎克雷伯菌及4株鲍曼不动杆菌,通过耐药性检测发现其中部分菌株具有对万古霉素、克林霉素、头孢唑啉和呋喃妥因等抗生素的多重耐药性;小鼠毒力试验表明分离菌株具备较强的毒力。本试验为奶牛场预防和治疗该类病原感染提供参考依据,同时对耐药菌株可能引起的公共卫生安全问题提出警示。
Klebsiella pneumoniae and Acinetobacter baumannii are important opportunistic pathogens causing infection in hospitals,and it's difficult to cure the people infected with multi-drug resistance strains.In this study,we isolated 3 Klebsiella pneumoniae strains and 4 Acinetobacter baumannii strains from raw milk at a dairy farm in Jiangsu province.Antibiotic-resistance analysis showed that some of these strains have multiple resistances against vancomycin,clindamycin,cefazolin or macrodantin.The experiments infecting the mice showed that the isolated strains have strong virulent to animals.The result of this study will give farms references for preventing and control disease caused by these pathogens,while drug resistance strains could also raise the caution for the potential problem of public health.
Klebsiella pneumoniae and Acinetobacter baumannii are important opportunistic pathogens causing infection in hospitals,and it's difficult to cure the people infected with multi-drug resistance strains.In this study,we isolated 3 Klebsiella pneumoniae strains and 4 Acinetobacter baumannii strains from raw milk at a dairy farm in Jiangsu province.Antibiotic-resistance analysis showed that some of these strains have multiple resistances against vancomycin,clindamycin,cefazolin or macrodantin.The experiments infecting the mice showed that the isolated strains have strong virulent to animals.The result of this study will give farms references for preventing and control disease caused by these pathogens,while drug resistance strains could also raise the caution for the potential problem of public health.
引文
[1]DIJKSHOORN L,NEMEC A,SEIFERT H.An increasing threat in hospitals:multidrug-resistant Acinetobacter baumannii[J].Nat Rev Microbiol,2007,5(12):939-951.
[2]GAO J,BARKEMA H W,ZHANG L,et al.Incidence of clinical mastitis and distribution of pathogens on large Chinese dairy farms[J].J Dairy Sci,2017,100(6):4797-4806.
[3]赵世媛,李永琴,刘溪源,等.奶牛肺炎克雷伯菌的分离鉴定[J].畜牧与兽医,2015,47(12):163-164.
[4]朱江巍,刘耀川,张雅为.奶牛乳房炎病原菌分离及药物敏感性试验[J].黑龙江畜牧兽医,2016(4):111-113.
[5]周玉龙,李国军,李阳,等.奶牛肺炎克雷伯氏菌的分离与鉴定[J].中国奶牛,2007(6):36-38.
[6]王亨,孟霞,邱昌伟,等.肺炎克雷伯氏菌对荷斯坦奶牛乳腺上皮细胞黏附和侵袭的体外研究[J].畜牧兽医学报,2008,39(4):494-498.
[7]周文君,孙裕光,沙莎.重庆某牛场乳房炎病原菌分离鉴定及药物敏感试验[J].中国兽医杂志,2014,50(10):73-76.
[8]王孝武,王旭荣,李建喜,等.牛源鲍曼不动杆菌的鉴定及体外药敏试验[J].西北农业学报,2015,24(5):13-17.
[9]李能章,邱荣蓉,牛娟,等.1株感染肉牛的鲍氏不动杆菌的分离与鉴定[J].安徽农业科学,2012(5):2717-2719.
[10]YANG Y,ZHOU M,HARDWIDGE P R,et al.Isolation and characterization of N-acyl homoserine lactone-producing bacteria from cattle rumen and swine intestines[J].Front Cell Infect Microbiol,2018(8):155.
[11]杨文丽,丁博,王俊斌,等.TA载体pEASY-T1Simple在基因克隆中的新应用[J].天津农学院学报,2011,18(2):13-15.
[12]李玲霞.肺炎克雷伯菌的临床分离及耐药性分析[D].吉林长春:吉林大学,2016.
[13]HALASA T,HUIJPS K,OSTERAS O,et al.Economic effects of bovine mastitis and mastitis management:a review[J].Vet Q,2007,29(1):18-31.
[14]张哲,李新圃,杨峰,等.奶牛隐性乳房炎综合防制措施的研究与应用[J].中国兽医学报,2018,38(3):598-601.
[15]WONG D,NIELSEN T B,BONOMO R A,et al.Clinical and pathophysiological overview of acinetobacter infections:a century of challenges[J].Clin Microbiol Rev,2017,30(1):409-447.
[16]金丹婷,茆海丰,刘洪书,等.1 056株鲍氏不动杆菌的分布及耐药谱分析[J].中华医院感染学杂志,2016,26(22):5067-5068.
[17]王伊伦,胡必杰,陈洪友,等.医院获得性鲍氏不动杆菌肺炎的流行病学研究[J].中华医院感染学杂志,2017,27(8):1681-1684.
[18]林华胜,张薇,李国明.肺炎克雷伯菌耐药机制的研究进展[J].中国医学创新,2014(12):151-154.
[19]WOODFORD N,TIERNO P M Jr,YOUNG K,et al.Outbreak of Klebsiella pneumoniae producing a new carbapenem-hydrolyzing class A beta-lactamase,KPC-3,in a New York medical center[J].Antimicrob A-gents Chemother,2004,48(12):4793-4799.
[20]吴华,周晓君,李天娇,等.2016年海南省高毒力肺炎克雷伯菌的临床分布、毒力基因和分子流行病学特点[J].中国感染控制杂志,2018,17(1):10-15.
[21]周梦兰,唐思,朱熙杰,等.多药耐药鲍氏不动杆菌医院感染最新研究进展[J].中华医院感染学杂志,2016,26(10):2398-2400.
[22]曾倩倩,袁喆.肺炎克雷伯菌3 276株耐药性监测分析[J].西部医学,2017,29(12):1698-1701.
[23]DEKIC′S,KLOBUCˇAR G,IVANKOVIC′T,et al.E-merging human pathogen Acinetobacter baumannii in the natural aquatic environment:apublic health risk[J].Int J Environ Health Res,2018,28(3):315.
[2]GAO J,BARKEMA H W,ZHANG L,et al.Incidence of clinical mastitis and distribution of pathogens on large Chinese dairy farms[J].J Dairy Sci,2017,100(6):4797-4806.
[3]赵世媛,李永琴,刘溪源,等.奶牛肺炎克雷伯菌的分离鉴定[J].畜牧与兽医,2015,47(12):163-164.
[4]朱江巍,刘耀川,张雅为.奶牛乳房炎病原菌分离及药物敏感性试验[J].黑龙江畜牧兽医,2016(4):111-113.
[5]周玉龙,李国军,李阳,等.奶牛肺炎克雷伯氏菌的分离与鉴定[J].中国奶牛,2007(6):36-38.
[6]王亨,孟霞,邱昌伟,等.肺炎克雷伯氏菌对荷斯坦奶牛乳腺上皮细胞黏附和侵袭的体外研究[J].畜牧兽医学报,2008,39(4):494-498.
[7]周文君,孙裕光,沙莎.重庆某牛场乳房炎病原菌分离鉴定及药物敏感试验[J].中国兽医杂志,2014,50(10):73-76.
[8]王孝武,王旭荣,李建喜,等.牛源鲍曼不动杆菌的鉴定及体外药敏试验[J].西北农业学报,2015,24(5):13-17.
[9]李能章,邱荣蓉,牛娟,等.1株感染肉牛的鲍氏不动杆菌的分离与鉴定[J].安徽农业科学,2012(5):2717-2719.
[10]YANG Y,ZHOU M,HARDWIDGE P R,et al.Isolation and characterization of N-acyl homoserine lactone-producing bacteria from cattle rumen and swine intestines[J].Front Cell Infect Microbiol,2018(8):155.
[11]杨文丽,丁博,王俊斌,等.TA载体pEASY-T1Simple在基因克隆中的新应用[J].天津农学院学报,2011,18(2):13-15.
[12]李玲霞.肺炎克雷伯菌的临床分离及耐药性分析[D].吉林长春:吉林大学,2016.
[13]HALASA T,HUIJPS K,OSTERAS O,et al.Economic effects of bovine mastitis and mastitis management:a review[J].Vet Q,2007,29(1):18-31.
[14]张哲,李新圃,杨峰,等.奶牛隐性乳房炎综合防制措施的研究与应用[J].中国兽医学报,2018,38(3):598-601.
[15]WONG D,NIELSEN T B,BONOMO R A,et al.Clinical and pathophysiological overview of acinetobacter infections:a century of challenges[J].Clin Microbiol Rev,2017,30(1):409-447.
[16]金丹婷,茆海丰,刘洪书,等.1 056株鲍氏不动杆菌的分布及耐药谱分析[J].中华医院感染学杂志,2016,26(22):5067-5068.
[17]王伊伦,胡必杰,陈洪友,等.医院获得性鲍氏不动杆菌肺炎的流行病学研究[J].中华医院感染学杂志,2017,27(8):1681-1684.
[18]林华胜,张薇,李国明.肺炎克雷伯菌耐药机制的研究进展[J].中国医学创新,2014(12):151-154.
[19]WOODFORD N,TIERNO P M Jr,YOUNG K,et al.Outbreak of Klebsiella pneumoniae producing a new carbapenem-hydrolyzing class A beta-lactamase,KPC-3,in a New York medical center[J].Antimicrob A-gents Chemother,2004,48(12):4793-4799.
[20]吴华,周晓君,李天娇,等.2016年海南省高毒力肺炎克雷伯菌的临床分布、毒力基因和分子流行病学特点[J].中国感染控制杂志,2018,17(1):10-15.
[21]周梦兰,唐思,朱熙杰,等.多药耐药鲍氏不动杆菌医院感染最新研究进展[J].中华医院感染学杂志,2016,26(10):2398-2400.
[22]曾倩倩,袁喆.肺炎克雷伯菌3 276株耐药性监测分析[J].西部医学,2017,29(12):1698-1701.
[23]DEKIC′S,KLOBUCˇAR G,IVANKOVIC′T,et al.E-merging human pathogen Acinetobacter baumannii in the natural aquatic environment:apublic health risk[J].Int J Environ Health Res,2018,28(3):315.