大豆加工品大豆黄卷和淡豆豉的质量评价
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摘要
目的对不同品种大豆(黑豆、青豆、黄豆、褐豆)为原料加工的大豆黄卷和淡豆豉进行含量测定,分析大豆黄卷和淡豆豉的实验室加工品和市售品中异黄酮含量的差异,为大豆黄卷和淡豆豉的制备研究及临床用药提供科学依据。方法以不同品种大豆为原料加工大豆黄卷和淡豆豉,采用紫外分光光度法测定总黄酮含量,使用HPLC法对药材中大豆苷、大豆苷元、染料木素、染料木苷含量进行测定。采用C_(18)色谱柱(250mm×4.6 mm,5μm),以甲醇-1%醋酸溶液为流动相,进行梯度洗脱,检测波长260 nm,柱温30℃,流速1.0 ml·min~(-1)。结果黄豆加工成的大豆黄卷中结合型糖苷大豆苷和染料木苷总量最大,含量范围为0.1090%～0.1431%,黑豆加工成的淡豆豉中游离型苷元大豆苷元和染料木素总量最大,含量范围为0.0937%～0.1628%。结论黄豆适宜加工成大豆黄卷,黑豆适宜加工淡豆豉,这也是市售品大豆黄卷和淡豆豉质量参差的原因。
Objective The study aimed to determine the contents of the Sojae Semen Germinatum and Sojae Semen Praeparatum.To analyze the difference of isoflavone content before and after processing of different varieties of Glycine max, and to provide scientific basis for the technological research and clinical application of Sojae Semen Germinatum and Sojae Semen Praeparatum. Methods UV spectrophotometry was used to determine total flavonoids, the contents of daidzin, daidzein, genistein, and genistin in the medicinal materials were determined by HPLC. Using C18 column(250 mm×4.6 mm, 5 μm),with a methanol-1% acetic acid solution as the mobile phase, a gradient elution was performed with a detection wavelength of 260 nm, a column temperature of 30 ℃, and a flow rate of 1.0 ml·min~(-1.) Results The largest total amount of daidzin and genistin in Sojae Semen Germinatum processed with yellow soybean was the highest, content range 0.1090% to 0.1431%. The content of free aglycones in Sojae Semen Praeparatum processed with black soybean was the highest, content range 0.0937% to 0.1628%. Conclusion Yellow soybean are suitable for the processing of Sojae Semen Germinatum, and black soybean are suitable for the processing of Sojae Semen Praeparatum, which is in line with the currently circulating on the market.
Objective The study aimed to determine the contents of the Sojae Semen Germinatum and Sojae Semen Praeparatum.To analyze the difference of isoflavone content before and after processing of different varieties of Glycine max, and to provide scientific basis for the technological research and clinical application of Sojae Semen Germinatum and Sojae Semen Praeparatum. Methods UV spectrophotometry was used to determine total flavonoids, the contents of daidzin, daidzein, genistein, and genistin in the medicinal materials were determined by HPLC. Using C18 column(250 mm×4.6 mm, 5 μm),with a methanol-1% acetic acid solution as the mobile phase, a gradient elution was performed with a detection wavelength of 260 nm, a column temperature of 30 ℃, and a flow rate of 1.0 ml·min~(-1.) Results The largest total amount of daidzin and genistin in Sojae Semen Germinatum processed with yellow soybean was the highest, content range 0.1090% to 0.1431%. The content of free aglycones in Sojae Semen Praeparatum processed with black soybean was the highest, content range 0.0937% to 0.1628%. Conclusion Yellow soybean are suitable for the processing of Sojae Semen Germinatum, and black soybean are suitable for the processing of Sojae Semen Praeparatum, which is in line with the currently circulating on the market.
引文
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[2] 李娜,黄庆柏.淡豆豉中的异黄酮成分及药理作用与临床应用[J].中国现代中药,2008,10(7):18.
[3] 葛喜珍,王鑫围,力提甫·斯拉木,等.中药淡豆豉有效成分大豆异黄酮调节血脂的研究进展[J].河北中医药学报.2002,17(3):41.
[4] 白霞,牛丽颖,刘姣.淡豆豉防治早期动脉粥样硬化大鼠血管损伤的机制研究[J].时珍国医国药,2008,19(1):170.
[5] 王爱梅,周建辉,欧阳静萍.大豆异黄酮对更年期大鼠抗氧化作用及提高学习记忆能力的研究[J].现代医药卫生,2008,24(7):954.
[6] Li N,Huang QB.The SSP isoflavone components and pharmacological effects and clinical applications[J]. Mod Chin Med,2008,10(7):18.
[7] 贾天纬,索瑶.大豆黄卷治愈宫颈癌1例报告[J].人民军医,1991,11:59.
[8] 刘秀玉,王利丽,左瑞庭,等.药用黑豆的研究进展[J].亚太传统医药,2017,13(20):83-84.
[9] 张蕊,姜义宝,杨玉荣,等.大豆异黄酮的特性及其应用研究进展[J].动物营养学报,2011, 23(11):1884.
[10] 阚厚远.中药材淡豆豉有效成分含量测定方法与生产工艺的研究[D].河北:河北大学硕士学位论文,2017:2.
[11] 李刚,梁永红,龙凯,等.再闷过程影响淡豆豉炮制工艺研究[J].中草药,2014,45(8):1083.
[12] 牛丽颖,石素琴,刘敏彦,等.淡豆豉炮制工艺的优化研究[J].中成药,2010, 32(8): 1372.
[13] 牛丽颖,杜红娜,刘姣,等.淡豆豉炮制前后异黄酮组分含量的比较[J].大豆科学,2008,27(4):672.
[14] 王鹏娇,吴运莉,张敏,等.21种不同产地中药淡豆豉中5种活性成分的对比[J].中国实验方剂学杂志,2014,20(19):64.
[15] 于密密,傅欣彤,郭洪祝.大豆黄卷质量控制方法研究[J].中国实验方剂学杂志,2013,19(4):103.
[16] 杨丹,王洪礼,马丹宁,等.不同发酵条件对淡豆豉中总异黄酮含量及蛋白酶活力的影响[J].中国现代中药,2016,18(7):826.
[17] 梁·陶弘景.尚志钧辑校.本草经集注[M].北京:人民卫生出版社,1994:502.
[18] 明·倪朱谟著,戴慎,陈仁寿,虞舜点校.本草汇言[M].上海:上海科学技术出版社,2005:869.
[19] 中国医学科学院药物研究所等编著.中药志[M].北京:人民卫生出版社,1984:131.
[20] 明·李时珍.本草纲目(校点本下册)[M].北京:人民卫生出版社,1982:1506-1508,1527.
[21] 王孝涛.历代中药炮制法汇典[M].南昌:江西科学技术出版社,1989:195,266.