风毛菊属5种植物的核型分析
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摘要
采用常规压片法,对风毛菊属(Saussurea)5种植物的染色体数目和核型类型进行分析。结果表明:大耳叶风毛菊(S.macrota)核型公式为2n=2x=26=10m+12sm+4st,属2A型;长梗风毛菊(S.dolichopoda)核型公式为2n=2x=26=14m+8sm+4st,属2A型;川陕风毛菊(S.licentiana)核型公式为2n=2x=28=12m+16sm,属2B型;杨叶风毛菊(S.populifolia)核型公式为2n=2x=28=6m+18sm+4st,属2B型;尾叶风毛菊(S.caudata)核型公式为2n=2x=30=14m+14sm+2st,属2A型。这5种风毛菊属植物中,除大耳叶风毛菊染色体数目和核型类型与前人报道的一致外,其余4种植物的染色体数目和核型类型均为首次报道,并在川陕风毛菊中发现1对B染色体。
The chromosome number and karyotypes of five Saussurea species were analyzed by traditional squash method.The result showed that:S.macrota was 2n=2x=26=10m+12sm+4st,karyotype belongs to stebbing 2Atype.S.dolichopoda was 2n=2x=26=14m+8sm+4st,karyotype belongs to stebbing 2A type.S.licentiana was 2n=2x=28=12m+16sm,karyotype belongs to stebbing 2Btype.S.populifolia was 2n=2x=28=6m+18sm+4st,karyotype belongs to stebbing 2Btype.S.caudata was 2n=2x=30=14m+14sm+2st,karyotype belongs to stebbing 2Atype.In these five species,the chromosome numbers and karyotype of S.macrotaspecies reconfirmed previous reports,while the chromosome numbers and karyotypes of other four species were presented in this paper for the first time,and a pair of B chromosome was discovered in S.licentiana.
The chromosome number and karyotypes of five Saussurea species were analyzed by traditional squash method.The result showed that:S.macrota was 2n=2x=26=10m+12sm+4st,karyotype belongs to stebbing 2Atype.S.dolichopoda was 2n=2x=26=14m+8sm+4st,karyotype belongs to stebbing 2A type.S.licentiana was 2n=2x=28=12m+16sm,karyotype belongs to stebbing 2Btype.S.populifolia was 2n=2x=28=6m+18sm+4st,karyotype belongs to stebbing 2Btype.S.caudata was 2n=2x=30=14m+14sm+2st,karyotype belongs to stebbing 2Atype.In these five species,the chromosome numbers and karyotype of S.macrotaspecies reconfirmed previous reports,while the chromosome numbers and karyotypes of other four species were presented in this paper for the first time,and a pair of B chromosome was discovered in S.licentiana.
引文
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[18]ZHOU Q X(周其兴),YANG Y P(杨永平),ZHANG M L(张明理).Karyotypes of fourteen species in Caragana[J].Bulletin of Botanical Research(植物研究),2002,22(4):492-496(in Chinese).
[19]WU G L.The application of karyotype analysis on cytotaxonomy[J].Journal of Biology,2006,23(1):39-42.
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[23]ARANO H.Cytological studies in subfamily Carduoideae(Compositae)of Japan IX[J].Botanical Magazine,1963,76:32-36.
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[26]LIU H CH(刘红昌),WANG ZH(王珍),ZHANG S Q(张素琴),et al.Analysis of karyotype and evolution trend of Polygonum multiflorum Thumb.gerplasm resources[J].Acta Bot.Boreal.-Occident.Sin.(西北植物学报),2013,33(6):1 123-1 136(in Chinese).
[27]RAO L B(饶龙兵),YANG H B(杨汉波),GUO H Y(郭洪英),et al.Karyotypes of seven species of Alnus in European and American Arease[J].Acta Bot.Boreal.-Occident.Sin.(西北植物学报),2013,33(7):1 333-1 338(in Chinese).
[28]GU ZH J(顾志建).Study on the origin of B-chromosome of Paris cronguistii[J].Acta Bot.Yunnan.(云南植物研究),1988,10(1):27-32(in Chinese).
[2]WANG Y F(王一峰),LIU Q Q(刘启茜),PEI Z Y(裴泽宇),et al.Correlation between altitude and reproductive allocation in three Saussureaspecies on China’s Qinghai-Tibetan Plateau[J].Chinese Journal of Plant Ecology(植物生态学报),2012,36(1):39-46(in Chinese).
[3]陈艺林,石铸.中国植物志(第78卷第2分册)[M].北京:科学出版社,1999:23-42.
[4]WANG Y J,SUSANNA A,VON R E,et al.Island-like radiation of Saussurea(Asteraceae:Cardueae)triggered by uplifts of the QinghaiTibetan Plateau[J].Biological Journal of the Linnean Society,2009,(97):893-903.
[5]WANG Y J,LIU J Q.A preliminary investigation on the phylogeny of Saussurea(Asteraceae:Cardueae)based on chloroplast DNAtrnLF sequences[J].Acta Phytotaxonomica Sinica,2004,(42):136-153.
[6]YUE X K(岳学坤),CAI J H(蔡金红),LI ZH M(李志敏),et al.Karyomorphology of the three species of Saussurea(Asteraceae)from Hengduan Mountains and the adjacent areas[J].Acta Botanica Yunnanica(云南植物研究),2008,30(4):433-436(in Chinese).
[7]TAI L H(邰丽华).A study on karyotypes of Saussurea runcinata[J].Journal of Inner Mongolia Normal University(Nat.Sci.Edi.)(内蒙古师大学报·自然科学版),1997,2(2):52-54(in Chinese).
[8]ZHOU M D(周明东),YAN P(阎平),LUO Q H(罗青红),et al.The karyotypes of Saussurea glacialis[J].Journal of Shihezi University(Nat.Sci.Edi.)(石河子大学学报·自然科学版),2005,23(1):78(in Chinese).
[9]LAVRENKO A N,SERDITOV N P,ULLE Z G.Chromosome numbers in some species of vascular plants from the Pechoro-llychsky Reservation(Komi ASSR)[J].Botanitcheskyi Zhurnal(Moscow&Leningrad),1991,76:473-476.
[10]PENG C I,HSU C C.Chromosome numbers in Taiwan Compositae[J].Botanical Bulletin of Academia Sinica(Taipei),1978,19:53-66.
[11]LIU Y H(刘玉红),WANG SH M(王善敏),WANG D J(王东江).Chromosome numbers of four species from Tibet,China[J].Acta Agrestia Sinica(草地学报),1994,2(2):55-57(in Chinese).
[12]AMANO M,OHBA H.Chromosome numbers of some alpine species of Saussurea(Asteraceae)in Nepal Himalaya[J].Journal of Japanese Botany,2000,7(3):178-184.
[13]FUJIKAWA K,IKEDA H,MURATA K,et al.Chromosome numbers of fifteen species of the genus Saussurea DC.(Asteraceae)in the Himalayas and the adjacent regions[J].Journal of Japanese Botany,2004,79(5):271-280.
[14]FUJIKAWA K,OHBA K.A cytological study of Saussureasubgenus eriocoryne(Asteraceae)from the Nepal Himalaya[J].Journal of Japanese Botany,2003,78(3):135-144.
[15]GAO T P(高天鹏),WANG ZH L(王转莉),GUO H Q(郭怀清),et al.Karyomorphology of 3 Saussureaspecies from the eastern of Qinghai Tibetan Plateau[J].Acta Pratacul Turae Sinica(草业学报),2009,18(2):169-174(in Chinese).
[16]CHEN G F(陈光富),BA L J(巴罗菊),SUN W G(孙文光),et al.Karyotypes and chromosome numbers of eight species from the family Asteraceae in the Hengduan Mountains and the adjacent regions[J].Plant Diversity and Resources(植物分类与资源学报),2013,35(3):367-374(in Chinese).
[17]WANG Y F(王一峰),WANG ZH L(王转莉),GONG H D(巩红冬),et al.Study on karyotypes of 4species of Saussurea DC.in the east of Qinghai Tibetan Plateau[J].Journal of Northwest A&F University(Nat.Sci.Edi.)(西北农林科技大学学报·自然科学版),2008,36(5):165-178(in Chinese).
[18]ZHOU Q X(周其兴),YANG Y P(杨永平),ZHANG M L(张明理).Karyotypes of fourteen species in Caragana[J].Bulletin of Botanical Research(植物研究),2002,22(4):492-496(in Chinese).
[19]WU G L.The application of karyotype analysis on cytotaxonomy[J].Journal of Biology,2006,23(1):39-42.
[20]李懋学,张赞平.作物染色体及其研究技术[M].北京:中国农业出版社,1996:1-39.
[21]LI M X(李懋学),CHEN D Y(陈端阳).The problem on karyotype analysis standardization[J].Journal of Wuhan Botanical Research(武汉植物研究),1985,3(4):297-302(in Chinese).
[22]LEVAN A,FREDGA K,SANDBERG A A.Nomenclacture for centromeric position on chromosomes[J].Hereditas,1964,52(2):201-220.
[23]ARANO H.Cytological studies in subfamily Carduoideae(Compositae)of Japan IX[J].Botanical Magazine,1963,76:32-36.
[24]STEBBINS G L.Chromosome Evolution in Higher Plants[M].London:Edward Aronold,1971:87-123.
[25]LI SH ZH(李双智),HE T SH(何廷顺),ZHANG D C(张大才),et al.Karyotypes of six species of genus Saussurea DC.in the Hengduan Mountains,Southweste China[J].Acta Bot.Boreal.-Occident.Sin.(西北植物学报),2013,33(11):2 194-2 202(in Chinese).
[26]LIU H CH(刘红昌),WANG ZH(王珍),ZHANG S Q(张素琴),et al.Analysis of karyotype and evolution trend of Polygonum multiflorum Thumb.gerplasm resources[J].Acta Bot.Boreal.-Occident.Sin.(西北植物学报),2013,33(6):1 123-1 136(in Chinese).
[27]RAO L B(饶龙兵),YANG H B(杨汉波),GUO H Y(郭洪英),et al.Karyotypes of seven species of Alnus in European and American Arease[J].Acta Bot.Boreal.-Occident.Sin.(西北植物学报),2013,33(7):1 333-1 338(in Chinese).
[28]GU ZH J(顾志建).Study on the origin of B-chromosome of Paris cronguistii[J].Acta Bot.Yunnan.(云南植物研究),1988,10(1):27-32(in Chinese).