毛蕊异黄酮对肺腺癌细胞增殖和迁移功能的影响
|
摘要
目的:检测毛蕊异黄酮对肺腺癌细胞生长转移的影响。方法:将不同浓度的毛蕊异黄酮分别作用于人肺癌细胞系A549细胞,通过MTT实验检测细胞增殖情况,通过transwell实验检测细胞转移情况。利用western blot及RT-PCR的方法检测毛蕊异黄酮对A549细胞相关蛋白的作用。结果:毛蕊异黄酮可以抑制A549细胞增殖与转移功能。(25、 50、100μg/mL)毛蕊异黄酮处理A549细胞24 h后,对其增殖的抑制率分别为(39.51±0.75)%,(48.91±3.64)%和(56.49±1.54)%。25μg/mL与50μg/mL毛蕊异黄酮作用A549细胞48 h后对其转移的抑制率分别为(17.76±3.82)%和(38.71±8.23)%。毛蕊异黄酮以剂量依赖的方式抑制A549细胞中CyclinD1与MMP2蛋白的表达。结论:毛蕊异黄酮可以通过抑制CyclinD1与MMP2蛋白的表达来抑制肺癌细胞A549细胞的增殖与转移。
Objective:To detect the effect of calycosin on the growth and metastasis of lung cancer cells.Methods:Different concentrations of calycosin were used to human lung cancer cell line A549 cells.The cell proliferation was detected by MTT assay.The cell metastasis was detected by Transwell assay.Using Western blot and RT-PCR were used to detect the effect of calycosin on A549 cells associated proteins.Results:Calycosin could inhibit the proliferation and metastasis of A549 cells(25,50,100 μg/mL)of calycosin were treated 24 hours on lung cells.The inhibition of proliferation was(39.51±0.75)%,(48.91±3.64)% and(56.49±1.54)%.The concentrations of 25 μg/mL and 50 μg/mL of calycosin were treated 24 hours on lung cells.The inhibition of migration was(17.76±3.82)% and(38.71±8.23)%.At the same time,calycosin can significantly inhibit the expressions of CyclinD1 and MMP2 in a dose-dependent manner.Conclusion:Calycosin can inhibit the proliferation and metastasis of lung cancer cell A549 cells by inhibiting the expression of CyclinD1 and MMP2.
Objective:To detect the effect of calycosin on the growth and metastasis of lung cancer cells.Methods:Different concentrations of calycosin were used to human lung cancer cell line A549 cells.The cell proliferation was detected by MTT assay.The cell metastasis was detected by Transwell assay.Using Western blot and RT-PCR were used to detect the effect of calycosin on A549 cells associated proteins.Results:Calycosin could inhibit the proliferation and metastasis of A549 cells(25,50,100 μg/mL)of calycosin were treated 24 hours on lung cells.The inhibition of proliferation was(39.51±0.75)%,(48.91±3.64)% and(56.49±1.54)%.The concentrations of 25 μg/mL and 50 μg/mL of calycosin were treated 24 hours on lung cells.The inhibition of migration was(17.76±3.82)% and(38.71±8.23)%.At the same time,calycosin can significantly inhibit the expressions of CyclinD1 and MMP2 in a dose-dependent manner.Conclusion:Calycosin can inhibit the proliferation and metastasis of lung cancer cell A549 cells by inhibiting the expression of CyclinD1 and MMP2.
引文
[1] Haeusler GM,Mechinaud F,Daley AJ,et al.Antibiotic-resistant gramnegative bacteremia in pediatric oncology patients-risk factors and outcomes[J].Pediatr Infect Dis J,2013,2 (7):723-726.
[2] 范越,杨云柯,马俊,等.松有饮联合化疗治疗60例胃癌患者的临床观察[J].中华中医药杂志,2007,22(7):467-469.
[3] Ohkawara S,Okuma Y,Uehara T,et al.Astraptero-carpan isolated from astragalus membr-anaceus inhibitsproliferation of vascular smooth muscle cells[J].Euro-pean Journal of Pharmacology,2005,525(1):41-47.
[4] Matsuda H,Yoshida K,Miyagawa K,et al.Rote-noids and flavonoids with anti-invasion of HT1080,an-ti-proliferation of U937 and differentiation-inducingactivity in HL-60 from erycibe expansa[J].Bioorganic& Medicinal Chemistry,2007,15(3):1539-1546.
[5] Umehara K,Nemoto K,Matsushita A,et al.Fla-vonoids from the heartwood of the Thai medicinal plantDalbergia parviflora and their effects on estrogenic-re-sponsive human brea-st cancer cell[J].Journal of Natural Products,2009,72(12):2163-2168.
[6] Zhou Y,Liu QH,Liu CL,et al.Calycosin induces apoptosis in human ovarian cancer SKOV3[J].Tumor Biol,2015,36 (7):5333-5339.
[7] 辛敏,陈建,郭艳红.毛蕊异黄酮对膀胱癌细胞凋亡和PI3K/Akt 信号通路的影响[J].广东医学,2015,36(5):690-693.
[8] CHEN J,HOU R,ZHANG X,et al.Calycosin suppresses breastcancer cell growth via ERβ-dependent regulation of IGF-1R,p38 MAPK and PI3K/Akt pathways[J].PLoS One,2014,9(3):e91245.
[9] 刘彬,刘慰华,张竞之,等.毛蕊异黄酮对人黑色素瘤细胞A375增殖、迁移及侵袭的影响[J].贵阳医学院学报,2014,39(2):162-166.
[10] Qiu R,Ma G,Zheng C,et al.Antineoplastic effect of calycosin on osteosarcoma through inducing apoptosis showing in vitro and in vivo investigations[J].Exp Mol Pathol,2014,97 (1):17-22.
[11] Zhang BY,Li YF,Lai Y,et al.[Effect of compound Chinese traditional medicine PC-SPES II in inhibiting proliferation of human prostate cancer cell LNCaP and on express-ions of AR and PSA].Zhongguo Zhong yao za zhi=Zhongguo zhongyao zazhi=China journal of Chinese materia medica,2015,40:950-956.
[12] Lin HC,Lin CL,Huang WY,et al.The use of adjunctive traditional Chinese medicine thera-py and survival outcome in patients with head and neck cancer:a nationwide population-based cohort study[J]. QJM:monthly journal of the Association of Physicians,2015,108:959-965.
[13] Bachmann K,Neumann A,Hinsch A,et al. Cyclin D1 is a strong prognostic factor for survival in pancreatic cancer:analysis of CD G870A polymorphism,FISH and immuno-histochemistry[J]. Journal of surgical oncology,2015,111:316-323.
[14] Gravina GL,Mancini A,Sanita P,et al. KPT-330,a potent and selective exportin-1(XPO-1)inhibitor,shows antitumor effects modulating the expression of cyclin D1 and surviving in prostate cancer models[J]. BMC cancer,2015,15:941.
[15] Bornschein J,Seidel T,Langner C,et al. MMP2 and MMP7 at the invasive front of gastric cancer are not associated with m TOR expression[J]. Diagnostic pathology,2015,10:212.
[16] Chen Q,Zhao X,Zhang H,et al. MiR-130b suppresses prostate cancer metastasis through down-regulation of MMP2[J]. Molecular carcinogenesis,2015,54:1292-1300.
[17] Kalhori V,Tornquist K. MMP2 and MMP9 participate in S1P-induced invasion of follicular ML-1 thyroid cancer cells[J]. Molecular and cellular endocrinology,2015,404:113-122.
[2] 范越,杨云柯,马俊,等.松有饮联合化疗治疗60例胃癌患者的临床观察[J].中华中医药杂志,2007,22(7):467-469.
[3] Ohkawara S,Okuma Y,Uehara T,et al.Astraptero-carpan isolated from astragalus membr-anaceus inhibitsproliferation of vascular smooth muscle cells[J].Euro-pean Journal of Pharmacology,2005,525(1):41-47.
[4] Matsuda H,Yoshida K,Miyagawa K,et al.Rote-noids and flavonoids with anti-invasion of HT1080,an-ti-proliferation of U937 and differentiation-inducingactivity in HL-60 from erycibe expansa[J].Bioorganic& Medicinal Chemistry,2007,15(3):1539-1546.
[5] Umehara K,Nemoto K,Matsushita A,et al.Fla-vonoids from the heartwood of the Thai medicinal plantDalbergia parviflora and their effects on estrogenic-re-sponsive human brea-st cancer cell[J].Journal of Natural Products,2009,72(12):2163-2168.
[6] Zhou Y,Liu QH,Liu CL,et al.Calycosin induces apoptosis in human ovarian cancer SKOV3[J].Tumor Biol,2015,36 (7):5333-5339.
[7] 辛敏,陈建,郭艳红.毛蕊异黄酮对膀胱癌细胞凋亡和PI3K/Akt 信号通路的影响[J].广东医学,2015,36(5):690-693.
[8] CHEN J,HOU R,ZHANG X,et al.Calycosin suppresses breastcancer cell growth via ERβ-dependent regulation of IGF-1R,p38 MAPK and PI3K/Akt pathways[J].PLoS One,2014,9(3):e91245.
[9] 刘彬,刘慰华,张竞之,等.毛蕊异黄酮对人黑色素瘤细胞A375增殖、迁移及侵袭的影响[J].贵阳医学院学报,2014,39(2):162-166.
[10] Qiu R,Ma G,Zheng C,et al.Antineoplastic effect of calycosin on osteosarcoma through inducing apoptosis showing in vitro and in vivo investigations[J].Exp Mol Pathol,2014,97 (1):17-22.
[11] Zhang BY,Li YF,Lai Y,et al.[Effect of compound Chinese traditional medicine PC-SPES II in inhibiting proliferation of human prostate cancer cell LNCaP and on express-ions of AR and PSA].Zhongguo Zhong yao za zhi=Zhongguo zhongyao zazhi=China journal of Chinese materia medica,2015,40:950-956.
[12] Lin HC,Lin CL,Huang WY,et al.The use of adjunctive traditional Chinese medicine thera-py and survival outcome in patients with head and neck cancer:a nationwide population-based cohort study[J]. QJM:monthly journal of the Association of Physicians,2015,108:959-965.
[13] Bachmann K,Neumann A,Hinsch A,et al. Cyclin D1 is a strong prognostic factor for survival in pancreatic cancer:analysis of CD G870A polymorphism,FISH and immuno-histochemistry[J]. Journal of surgical oncology,2015,111:316-323.
[14] Gravina GL,Mancini A,Sanita P,et al. KPT-330,a potent and selective exportin-1(XPO-1)inhibitor,shows antitumor effects modulating the expression of cyclin D1 and surviving in prostate cancer models[J]. BMC cancer,2015,15:941.
[15] Bornschein J,Seidel T,Langner C,et al. MMP2 and MMP7 at the invasive front of gastric cancer are not associated with m TOR expression[J]. Diagnostic pathology,2015,10:212.
[16] Chen Q,Zhao X,Zhang H,et al. MiR-130b suppresses prostate cancer metastasis through down-regulation of MMP2[J]. Molecular carcinogenesis,2015,54:1292-1300.
[17] Kalhori V,Tornquist K. MMP2 and MMP9 participate in S1P-induced invasion of follicular ML-1 thyroid cancer cells[J]. Molecular and cellular endocrinology,2015,404:113-122.