矮石斛化学成分研究
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摘要
目的研究兰科石斛属植物矮石斛Dendrobium bellatulum的化学成分。方法采用各种柱色谱及制备液相色谱分离纯化,经波谱数据鉴定化合物结构。结果从矮石斛全草甲醇提取物中分离得到15个化合物,分别鉴定为2-羟基-4-甲氧基-3,6-二甲基苯甲酸(1)、4′,5-二羟基-3,3′-二甲氧基联苄(2)、3,3′-二羟基-4,5-二甲氧基联苄(3)、二氢松柏醇二氢对羟基桂皮酸酯(4)、aloifolI(5)、山药素Ⅲ(6)、dendrosinenB(7)、2,5,7-三羟基-4-甲氧基-9,10-二氢菲(8)、对羟基苯丙酸(9)、对羟基肉桂酸(10)、阿魏酸(11)、咖啡酸(12)、dendrosinen D(13)、新橄榄树脂素(14)、3-羟甲基-9-甲氧基-2-(4′-羟基-3′,5′-二甲氧基苯基)-2,3,6,7-四氢菲[4,3-b]呋喃-5,11-二醇(15)。结论化合物1～15均为首次从该植物中分离得到,其中化合物1为首次从兰科植物中分离得到。
Objective To study the chemical constituents of Dendrobium bellatulum. Methods The compounds were isolated and purified by column chromatography and preparative HPLC, and their structures were elucidated by spectral analysis. Results Fifteen compounds were isolated and identified as 2-hydroxy-4-methoxy-3,6-dimethylbenzoic acid(1), 4′,5-dihydroxy-3,3′-dimethoxybiphezyl(2), 3,3′-dihydroxy-4,5-dimethoxybiphezyl(3), dihydroconiferyl dihydro-p-coumarate(4), aloifol I(5), batatasin Ⅲ(6), dendrosinen B(7), 2,5,7-trihydroxy-4-methoxy-9,10-dihydrophenanthrene(8), p-hydroxyphenyl-propionic acid(9),p-hydroxycinnamic acid(10), ferulic acid(11), caffeic acid(12), dendrosinen D(13), neoolivil(14), and 3-hydroxymethyl-9-methoxy-2-(4′-hydroxy-3′,5′-dimethoxyphenyl)-2,3,6,7-tetrahydrophenanthro [4,3-b] furan-5,11-diol(15). Conclusion All compounds are isolated from D. bellatulum for the first time, and compound 1 is isolated from the family Orchidaceae for the first time.
Objective To study the chemical constituents of Dendrobium bellatulum. Methods The compounds were isolated and purified by column chromatography and preparative HPLC, and their structures were elucidated by spectral analysis. Results Fifteen compounds were isolated and identified as 2-hydroxy-4-methoxy-3,6-dimethylbenzoic acid(1), 4′,5-dihydroxy-3,3′-dimethoxybiphezyl(2), 3,3′-dihydroxy-4,5-dimethoxybiphezyl(3), dihydroconiferyl dihydro-p-coumarate(4), aloifol I(5), batatasin Ⅲ(6), dendrosinen B(7), 2,5,7-trihydroxy-4-methoxy-9,10-dihydrophenanthrene(8), p-hydroxyphenyl-propionic acid(9),p-hydroxycinnamic acid(10), ferulic acid(11), caffeic acid(12), dendrosinen D(13), neoolivil(14), and 3-hydroxymethyl-9-methoxy-2-(4′-hydroxy-3′,5′-dimethoxyphenyl)-2,3,6,7-tetrahydrophenanthro [4,3-b] furan-5,11-diol(15). Conclusion All compounds are isolated from D. bellatulum for the first time, and compound 1 is isolated from the family Orchidaceae for the first time.
引文
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[4]张光浓,毕志明,王峥涛,等.石斛属植物化学成分研究进展[J].中草药,2003,34(6):5-8.
[5]林萍,毕志明,徐红,等.石斛属植物药理活性研究进展[J].中草药,2003,34(2):19-22.
[6]肖世基,刘珍,张茂生,等.金钗石斛中一个新的联苄类化合物[J].药学学报,2016,51(7):1117-1120.
[7]肖世基,钱怡,张良,等.黔产金钗石斛中1个新的杜松烷型倍半萜[J].中草药,2016,47(17):2972-2974.
[8]王峰,池翠云,何翠红,等.圆盖阴石蕨的化学成分研究[J].中成药,2011,33(4):645-648.
[9]管惠娟,张雪,屠凤娟,等.铁皮石斛化学成分的研究[J].中草药,2009,40(12):1873-1876.
[10]Arriaga-Giner F J,Wollenweber E,Dorr M,et al.Bibenzyls from crowberry leaves[J].Phytochemistry,1993,33(3):725-726.
[11]汪代芳,侴桂新,赵宁毅,等.金钗石斛茎的化学成分研究[J].中草药,2012,43(8):1492-1495.
[12]Juneja R K,Sharma S C,Tandon J S.Two substituted bibenzyls and adihydrophenanthrene from Cymbidium aloifolium[J].Phytochemistry,1987,26(4):1123-1125.
[13]Chen X J,Mei W L,Cai C H,et al.Four new bibenzyl derivatives from Dendrobium sinense[J].Phytochem Lett,2014,57(9):107-112.
[14]Hu J M,Chen J J,Yu H,et al.Five new compounds from Dendrobium longicornu[J].Planta Med,2008,74(5):535-539.
[15]纪明昌,郭大乐,肖世基,等.石柑子中酚酸类化学成分研究[J].天然产物研究与开发,2015,27(4):609-612.
[16]冯美玲,王书芳,张兴贤.枸杞子的化学成分研究[J].中草药,2013,44(3):265-268.
[17]何春年,王春兰,郭顺星,等.福建金线莲的化学成分研究Ⅱ[J].中国中药杂志,2005,30(10):761-763.
[18]邹旭,梁健,丁立生,等.鸡屎藤化学成分的研究[J].中国中药杂志,2006,31(17):1436-1441.
[19]刘栋,张建,汤少男,等.臭椿皮中木脂素类成分研究[J].中国中药杂志,2016,41(24):4615-4620.
[20]Leong Y W,Harrison L J.A biphenanthrene and a phenanthro[4,3-b]furan from the Orchid Bulbophyllum vaginatum[J].J Nat Prod,2004,67(9):1601-1603.