清补通络浓缩丸提取工艺优化研究
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摘要
目的优选清补通络浓缩丸提取工艺。方法采用高效液相色谱(HPLC)法测定黄芩苷含量,以黄芩苷含量及出膏率为考察指标,正交试验优选最佳提取工艺。结果最佳提取工艺为料液比1∶10,提取2次,每次1 h;在此条件下,黄芩苷含量为4.49 mg/g,出膏率为4.84%。结论优选的清补通络浓缩丸提取工艺合理、稳定、效果良好,可为清补通络浓缩丸及其他中药新药的生产提供试验依据。
Objective To optimize the extraction process of Qingbutongluo Concentrated Pills. Methods The content of baicalin was determined by HPLC,and the optimum extraction process was optimized by orthogonal test with the content of baicalin and the yield of extraction as the indexes. Results The optimum extraction process was as follows: the ratio of material to liquid was 1 ∶ 10 with 2 times of extraction,1 h each time. Under this condition,the content of baicalin was 4. 49 mg/g,and the yield of extraction was 4. 84%.Conclusion The optimized extraction process is reasonable,stable and effective,which can provide the experimental basis for the production of Qingbutongluo Concentrated Pills and other new medicines of traditional Chinese medicine.
Objective To optimize the extraction process of Qingbutongluo Concentrated Pills. Methods The content of baicalin was determined by HPLC,and the optimum extraction process was optimized by orthogonal test with the content of baicalin and the yield of extraction as the indexes. Results The optimum extraction process was as follows: the ratio of material to liquid was 1 ∶ 10 with 2 times of extraction,1 h each time. Under this condition,the content of baicalin was 4. 49 mg/g,and the yield of extraction was 4. 84%.Conclusion The optimized extraction process is reasonable,stable and effective,which can provide the experimental basis for the production of Qingbutongluo Concentrated Pills and other new medicines of traditional Chinese medicine.
引文
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[2]吴冬玲.布鲁氏菌Vir BS-PCR诊断方法的建立与应用及Ery基因的克隆与序列分析[D].乌鲁木齐:新疆农业大学,2008.
[3]何凤田.布氏杆菌新型疫苗的构建及保护效果的研究[D].重庆:中国人民解放军第三军医大学,2005.
[4]王季秋,崔京玉.布鲁氏菌病治疗研究进展[J].中国地方病防治杂志,2007,22(2):104-106.
[5]胡剑飞.布鲁菌的分离鉴定及omp31基因的克隆和序列分析[D].呼和浩特:内蒙古农业大学,2010.
[6]尚德秋.布氏菌病研究进展[J].中国地方病防治杂志,2004,19(4):204-212.
[7]肖东楼,冶森林,王大力,等.布鲁氏菌病防治手册[M].北京:人民卫生出版社,2008:9.
[8]洪铁,杨振,绳娟,等.黄芩苷抗肿瘤作用及机制的研究[J].中国药理学通报,2008,24(12):1676-1678.
[9]侯艳宁,朱秀媛,程桂芳.黄芩苷的抗炎机理[J].药学学报,2000,35(3):161-164.
[10]吴梅,闫慧,张春梅.黄芩苷作为一种抗生物制剂的过敏反应研究[J].微生物学免疫学进展,2007,35(4):48-50.
[11]陈丽平.黄芩有效化学成分及抑菌作用的研究分析[J].中国实用医药,2011,6(10):167.
[12]国家药典委员会.中华人民共和国药典(一部)[M].北京:中国医药科技出版社,2015:301-302.
[13]陈克克,王吉吉之.党参多糖的研究进展[J].现代生物医学进展,2007,7(4):635-636.