小球藻高效净化猪场废水厌氧发酵沼液研究
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摘要
为了净化脱除养猪场废水厌氧发酵沼液中的主要污染物,本文利用臭氧氧化对沼液灭菌并且降解其中的大分子有机物(效果优于高压蒸汽灭菌法),然后高效培养小球藻转化为生物质变废为宝.研究了通入不同体积浓度CO_2以及在沼液中补充部分营养盐对微藻生长净化沼液的影响.当CO_2体积浓度从2%逐渐增加到30%时,微藻生物质产量和脱除污染物效率先升后降,并在体积浓度为15%时达到峰值.当沼液中补充添加营养盐10 mg·L~(-1)磷和1 mg·L~(-1)铁时,微藻生物质产量相对未添加条件进一步提高了29.1%,达到了5.81 g·L~(-1),相应的COD、TN、NH_3-N、TP和抗生素替米考星等污染物脱除效率分别提高到88.5%、91.2%、93.6%、98.9%和90.7%.因此,小球藻可高效净化猪场厌氧发酵沼液同时生产高值生物质,具有良好的应用前景.
In order to remove the pollutants in anaerobic digestion effluent of hoggery wastewater, ozonation method was used to sterilize the effluent and degrade macromolecular organics simultaneously. It was proved to be a better sterilization method than autoclave. The sterilized anaerobic digestion effluent was then used to cultivate Chlorella efficiently and the pollutants were transformed to biomass with high value. The effects of CO_2 volume concentrations and nutrients supplementation on microalgal growth and pollutants removal were studied. When CO_2 volumatric concentration gradually increased from 2% to 30%, microalgal biomass yield and pollutants removal efficiency first increased and then decreased. The peak value reached at the 15% of CO_2 volume concentration. When 10 mg·L~(-1) phosphorus and 1 mg·L~(-1) iron were added into the anaerobic digestion effluent, the biomass yield of microalgae increased by 29.1% to 5.81 g·L~(-1). The removal efficiencies of COD, NH_3-N, TN, TP and antibiotic tilmicosin increased to 88.5%, 93.6%, 91.2%, 98.9%, and 90.7%, respectively. Therefore, Chlorella can effectively purify the anaerobic digestion effluent of hoggery wastewater and produce high-value biomass at the same time, which exhibits good application prospect.
In order to remove the pollutants in anaerobic digestion effluent of hoggery wastewater, ozonation method was used to sterilize the effluent and degrade macromolecular organics simultaneously. It was proved to be a better sterilization method than autoclave. The sterilized anaerobic digestion effluent was then used to cultivate Chlorella efficiently and the pollutants were transformed to biomass with high value. The effects of CO_2 volume concentrations and nutrients supplementation on microalgal growth and pollutants removal were studied. When CO_2 volumatric concentration gradually increased from 2% to 30%, microalgal biomass yield and pollutants removal efficiency first increased and then decreased. The peak value reached at the 15% of CO_2 volume concentration. When 10 mg·L~(-1) phosphorus and 1 mg·L~(-1) iron were added into the anaerobic digestion effluent, the biomass yield of microalgae increased by 29.1% to 5.81 g·L~(-1). The removal efficiencies of COD, NH_3-N, TN, TP and antibiotic tilmicosin increased to 88.5%, 93.6%, 91.2%, 98.9%, and 90.7%, respectively. Therefore, Chlorella can effectively purify the anaerobic digestion effluent of hoggery wastewater and produce high-value biomass at the same time, which exhibits good application prospect.
引文
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孙建平,郑平,胡宝兰,等.2009.抗生素对猪场废水厌氧生物处理的影响[J].太阳能学报,30(6):824-828
Vaiopoulou E,Misiti T M,Pavlostathis S G.2015.Removal and toxicity reduction of naphthenic acids by ozonation and combined ozonation-aerobic biodegradation [J].Bioresource Technology,179:339-347
Wang L,Li Y,Chen P,et al.2010.Anaerobic digested dairy manure as a nutrient supplement for cultivation of oil-rich green microalgae Chlorella sp.[J].Bioresource Technology,101(8):2623-2628
王愿珠,程鹏飞,刘德富,等.2017.生物膜贴壁培养小球藻净化猪粪沼液废水的效果 [J].环境科学,38(8):3354-3361
杨熙,向文洲,黄颖华,等.2017.CO2浓度对嗜碱绿球藻MC-1光合作用和生长的影响 [J].江苏农业科学,45(18):158-162
于洪贤,孙旭,马成学.2016.Fe3+浓度对淡水蛋白核小球藻Chlorella pyrenodiosa生长的影响 [J].水产学杂志,29(1):34-37
Cheng J,Huang Y,Feng J,et al.2013.Mutate Chlorella sp by nuclear irradiation to fix high concentrations of CO2 [J].Bioresource Technology,136:496-501
Cheng J,Xu J,Huang Y,et al.2015.Growth optimisation of microalga mutant at high CO2 concentration to purify undiluted anaerobic digestion effluent of swine manure [J].Bioresource Technology,177:240-246
程军,许姣,冯佳,等.2016.菱形藻同时自养和异养提高油脂产率的研究[J].太阳能学报,37(7):1664-1669
胡桂英,高巍,王薇.2009.臭氧消毒灭菌技术的进展 [Z].中国河南郑州.20092
Ji M,Yun H,Park S,et al.2015.Effect of food wastewater on biomass production by a green microalga Scenedesmus obliquus for bioenergy generation [J].Bioresource Technology,179:624-628
Levine R B,Costanza-Robinson M S,Spatafora G A.2011.Neochloris oleoabundans grown on anaerobically digested dairy manure for concomitant nutrient removal and biodiesel feedstock production [J].Biomass & Bioenergy,35(1):40-49
李夜光,李中奎,耿亚红,等.2006.富营养化水体中N、P浓度对浮游植物生长繁殖速率和生物量的影响 [J].生态学报,26(2):317-325
Park J,Jin H,Lim B,et al.2010.Ammonia removal from anaerobic digestion effluent of livestock waste using green alga Scenedesmus sp.[J].Bioresource Technology,101(22):8649-8657
Singh M,Reynolds D L,Das K C.2011.Microalgal system for treatment of effluent from poultry litter anaerobic digestion [J].Bioresource Technology,102(23):10841-10848
孙建平,郑平,胡宝兰,等.2009.抗生素对猪场废水厌氧生物处理的影响[J].太阳能学报,30(6):824-828
Vaiopoulou E,Misiti T M,Pavlostathis S G.2015.Removal and toxicity reduction of naphthenic acids by ozonation and combined ozonation-aerobic biodegradation [J].Bioresource Technology,179:339-347
Wang L,Li Y,Chen P,et al.2010.Anaerobic digested dairy manure as a nutrient supplement for cultivation of oil-rich green microalgae Chlorella sp.[J].Bioresource Technology,101(8):2623-2628
王愿珠,程鹏飞,刘德富,等.2017.生物膜贴壁培养小球藻净化猪粪沼液废水的效果 [J].环境科学,38(8):3354-3361
杨熙,向文洲,黄颖华,等.2017.CO2浓度对嗜碱绿球藻MC-1光合作用和生长的影响 [J].江苏农业科学,45(18):158-162
于洪贤,孙旭,马成学.2016.Fe3+浓度对淡水蛋白核小球藻Chlorella pyrenodiosa生长的影响 [J].水产学杂志,29(1):34-37